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The map label for this gene is bioH [H]

Identifier: 158423008

GI number: 158423008

Start: 1566127

End: 1566954

Strand: Direct

Name: bioH [H]

Synonym: AZC_1384

Alternate gene names: 158423008

Gene position: 1566127-1566954 (Clockwise)

Preceding gene: 158423007

Following gene: 158423009

Centisome position: 29.17

GC content: 66.3

Gene sequence:

>828_bases
ATGTCCAAAAGCCTTGAACTCGCGCCGACCCTGTATGTGGAAGCGGCCGGAATCCGCTTTGCCTATCGGCGGCTGGGACC
GGACAGCGGGGTGCCCCTCGTGCTGCTCCAGCATTTCACCGGCACCATGGATGCGTGGGACCCGGCGGTGGTCAATGCCC
TGGCGCAGGACCGGCCGGTGATCGCGTTCGATAATACGGGCGTCGGGTCTTCCTCCGGCGAGACGCCGGATACGGTGGAG
CAGCAGGCGGCGGACGCGGAAGCGTTCATCGTGGCTCTCGGCCTGAGGGAGGTGGATCTCCTTGGCTTCTCGCTCGGCGG
GTTTCTGGTGCAGGTCATGGCTGGGCGTAACGCCGTGAAGGTTCGCCGCATCATCCTTGCCGGCACCGCCCCGCGGGGAG
GCGAGGAGCATCTGCTCCAGGTGGTCGACGAGGCCTTCGCCCGCAACGCCCGCGATGTGCGGCTGCCGCTGTTCTTCACG
CCGTCCGACGCCAGCCAGGCCGCCGGTGCTGCCTTCGTCGACCGCGCGATGCTGCGGCAGGCGGATCGCGATCCCGACCG
CGGCGAGGCGGTCAGCACGCCTCAGGCCCAGGCGATCATCCGCTGGTGCGCGGAAGCGGACGGGCACGCGACGCTCCGGG
CAATCCGGCAGCCGACGCTCATCGTTCATGGCAGCGACGACACCATGTTTCCGTCCATCAATGCCTACGAGATGTTCAAG
AACATGAGCAGCGCGACGCTCATTCTCTACCCCGATTCCGCCCACGGCGCCCTGTTTCAGTATCCCGAAACATTCGTATC
CCACGTCAAGACGTTCCTTGACGCCTGA

Upstream 100 bases:

>100_bases
CGTGAAGGGCTGATCAACGGACGGCGGCGCCATGCCGGAGTGCCGCCGTCTCCGAACGCGACTGGCAATTGACCTCATGT
GGAATGGGGAAGGATGACGC

Downstream 100 bases:

>100_bases
TGCCAGACAAGAACTGAGAGGATAGGCTTATGGATCCGCTGGCTCAAAAGATCATCGCCGCCCATGGCGGGCTCGACCGG
TGGCGTACCTTCACCCAGCT

Product: hydrolase

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSKSLELAPTLYVEAAGIRFAYRRLGPDSGVPLVLLQHFTGTMDAWDPAVVNALAQDRPVIAFDNTGVGSSSGETPDTVE
QQAADAEAFIVALGLREVDLLGFSLGGFLVQVMAGRNAVKVRRIILAGTAPRGGEEHLLQVVDEAFARNARDVRLPLFFT
PSDASQAAGAAFVDRAMLRQADRDPDRGEAVSTPQAQAIIRWCAEADGHATLRAIRQPTLIVHGSDDTMFPSINAYEMFK
NMSSATLILYPDSAHGALFQYPETFVSHVKTFLDA

Sequences:

>Translated_275_residues
MSKSLELAPTLYVEAAGIRFAYRRLGPDSGVPLVLLQHFTGTMDAWDPAVVNALAQDRPVIAFDNTGVGSSSGETPDTVE
QQAADAEAFIVALGLREVDLLGFSLGGFLVQVMAGRNAVKVRRIILAGTAPRGGEEHLLQVVDEAFARNARDVRLPLFFT
PSDASQAAGAAFVDRAMLRQADRDPDRGEAVSTPQAQAIIRWCAEADGHATLRAIRQPTLIVHGSDDTMFPSINAYEMFK
NMSSATLILYPDSAHGALFQYPETFVSHVKTFLDA
>Mature_274_residues
SKSLELAPTLYVEAAGIRFAYRRLGPDSGVPLVLLQHFTGTMDAWDPAVVNALAQDRPVIAFDNTGVGSSSGETPDTVEQ
QAADAEAFIVALGLREVDLLGFSLGGFLVQVMAGRNAVKVRRIILAGTAPRGGEEHLLQVVDEAFARNARDVRLPLFFTP
SDASQAAGAAFVDRAMLRQADRDPDRGEAVSTPQAQAIIRWCAEADGHATLRAIRQPTLIVHGSDDTMFPSINAYEMFKN
MSSATLILYPDSAHGALFQYPETFVSHVKTFLDA

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 29660; Mature: 29529

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLELAPTLYVEAAGIRFAYRRLGPDSGVPLVLLQHFTGTMDAWDPAVVNALAQDRPV
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCE
IAFDNTGVGSSSGETPDTVEQQAADAEAFIVALGLREVDLLGFSLGGFLVQVMAGRNAVK
EEECCCCCCCCCCCCCHHHHHHHCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHE
VRRIILAGTAPRGGEEHLLQVVDEAFARNARDVRLPLFFTPSDASQAAGAAFVDRAMLRQ
EEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
ADRDPDRGEAVSTPQAQAIIRWCAEADGHATLRAIRQPTLIVHGSDDTMFPSINAYEMFK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHH
NMSSATLILYPDSAHGALFQYPETFVSHVKTFLDA
CCCCCEEEEECCCCCCCHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSLELAPTLYVEAAGIRFAYRRLGPDSGVPLVLLQHFTGTMDAWDPAVVNALAQDRPV
CCCCCCCCEEEEEECCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCE
IAFDNTGVGSSSGETPDTVEQQAADAEAFIVALGLREVDLLGFSLGGFLVQVMAGRNAVK
EEECCCCCCCCCCCCCHHHHHHHCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHE
VRRIILAGTAPRGGEEHLLQVVDEAFARNARDVRLPLFFTPSDASQAAGAAFVDRAMLRQ
EEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
ADRDPDRGEAVSTPQAQAIIRWCAEADGHATLRAIRQPTLIVHGSDDTMFPSINAYEMFK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHH
NMSSATLILYPDSAHGALFQYPETFVSHVKTFLDA
CCCCCEEEEECCCCCCCHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]