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The map label for this gene is ribH

Identifier: 158422889

GI number: 158422889

Start: 1415468

End: 1415956

Strand: Direct

Name: ribH

Synonym: AZC_1265

Alternate gene names: 158422889

Gene position: 1415468-1415956 (Clockwise)

Preceding gene: 158422888

Following gene: 158422890

Centisome position: 26.36

GC content: 69.73

Gene sequence:

>489_bases
ATGGTAAGCACGCGCAAGGAAGGCGCGAGCGCCGGGGAGCCCGTCACCGGGGCCCGAGTGCTGATTGTGGAAGGGCGCTA
TTACGAGGCGCTGGCGGACGAGCTTCTGGCCGGCGCTCGCGCCGCGCTGGAAGCGGCCGGTGCCACCGTGGACGTGATCA
CTGTTCCGGGTGCGCTGGAAATCCCGATCGCCGCCGAGATCGCCCTCGAGGCGGCCGAGGAAGAGGACGAGCCTTACGAG
GCAGTGGTGGCGCTCGGCTGCGTCATCCGCGGCGAGACCTACCATTTCGAGATCGTGGCGGGAGAATCCTCGCGCGGCCT
CATGGATCTGGCGCTCGCCCATGCCCTGCCGCTCGGCAATGGCATCCTGACCGTGGAAACCGAGGCGCAGGCCTGGGAGC
GGGCGCGGGTGGGCGAGGGCAACAAGGGCGGCGGTGCCGCCGAAGCCGCTTTGAGCCTGGTGCGCCTGAAGCGCCGGGGC
CTCAAATGA

Upstream 100 bases:

>100_bases
CTCTCCATTTGAAATCGGAGCCGGATTTCCCGGGCAGAAGGATTTCCTCTGACCCCTTCCGGCTCTAGTACCTGACGCGC
CCGAATCGGAGAACATCAAG

Downstream 100 bases:

>100_bases
GCGATCAGGTCTCCAAGCCCATGCGCAAGAGCGCGGCCCGTCTCGCGGCGTGCAGGCGCTCTACCAGATGGACATCGCCG
GCACGCCGGTGAACGAGATC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MVSTRKEGASAGEPVTGARVLIVEGRYYEALADELLAGARAALEAAGATVDVITVPGALEIPIAAEIALEAAEEEDEPYE
AVVALGCVIRGETYHFEIVAGESSRGLMDLALAHALPLGNGILTVETEAQAWERARVGEGNKGGGAAEAALSLVRLKRRG
LK

Sequences:

>Translated_162_residues
MVSTRKEGASAGEPVTGARVLIVEGRYYEALADELLAGARAALEAAGATVDVITVPGALEIPIAAEIALEAAEEEDEPYE
AVVALGCVIRGETYHFEIVAGESSRGLMDLALAHALPLGNGILTVETEAQAWERARVGEGNKGGGAAEAALSLVRLKRRG
LK
>Mature_162_residues
MVSTRKEGASAGEPVTGARVLIVEGRYYEALADELLAGARAALEAAGATVDVITVPGALEIPIAAEIALEAAEEEDEPYE
AVVALGCVIRGETYHFEIVAGESSRGLMDLALAHALPLGNGILTVETEAQAWERARVGEGNKGGGAAEAALSLVRLKRRG
LK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=145, Percent_Identity=43.448275862069, Blast_Score=94, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324429, Length=126, Percent_Identity=30.1587301587302, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_AZOC5 (A8I0J5)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001524181.1
- ProteinModelPortal:   A8I0J5
- SMR:   A8I0J5
- GeneID:   5690893
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1265
- HOGENOM:   HBG311126
- OMA:   SSRALMD
- ProtClustDB:   PRK00061
- BioCyc:   ACAU438753:AZC_1265-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16778; Mature: 16778

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTRKEGASAGEPVTGARVLIVEGRYYEALADELLAGARAALEAAGATVDVITVPGALE
CCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
IPIAAEIALEAAEEEDEPYEAVVALGCVIRGETYHFEIVAGESSRGLMDLALAHALPLGN
CCCHHHHHHHHHCCCCCHHHHHHHHHHEEECCEEEEEEEECCCCCCHHHHHHHHHCCCCC
GILTVETEAQAWERARVGEGNKGGGAAEAALSLVRLKRRGLK
CEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSTRKEGASAGEPVTGARVLIVEGRYYEALADELLAGARAALEAAGATVDVITVPGALE
CCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
IPIAAEIALEAAEEEDEPYEAVVALGCVIRGETYHFEIVAGESSRGLMDLALAHALPLGN
CCCHHHHHHHHHCCCCCHHHHHHHHHHEEECCEEEEEEEECCCCCCHHHHHHHHHCCCCC
GILTVETEAQAWERARVGEGNKGGGAAEAALSLVRLKRRGLK
CEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA