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The map label for this gene is glyA [H]

Identifier: 158422885

GI number: 158422885

Start: 1411482

End: 1412795

Strand: Direct

Name: glyA [H]

Synonym: AZC_1261

Alternate gene names: 158422885

Gene position: 1411482-1412795 (Clockwise)

Preceding gene: 158422884

Following gene: 158422886

Centisome position: 26.29

GC content: 67.12

Gene sequence:

>1314_bases
ATGGCCTCGTCCGCTGCGCACAGCGCCTCCGCCTCGGCGGAGATGAACCGCTTCTTCTCCGCCTCCCTCGCGGAGGTTGA
TCCCGAGATCGCCGCCGCCGTGTCGGCCGAACTCGGTCGCCAGCGCGAGGAAATCGAGCTCATCGCCTCGGAAAACATCG
TCTCCCGCGCCGTGCTCGAGGCCCAGGGCTCGGTGCTCACCAACAAGTACGCCGAGGGCTATCCGGGCAAGCGCTATTAT
GGCGGCTGCCAGTTCGTGGACGTGGCCGAGAACCTCGCCATCGATCGCGCCAAGAAGCTGTTCGGCTGCGCCTTCGCGAA
CGTACAGCCGAATTCCGGCAGCCAGGCCAATCAGGGCGTCTTCTTCACCCTGATGCAGCCGGGCGACACCTTCCTCGGCC
TCAACCTCGCCGCCGGCGGCCATCTGACCCATGGCTCGCCGGTCAACATGTCGGGCAAGTGGTTCAACGCGGTGGCCTAT
GGCGTGCGCGAGGACGACCAGCGCATTGATTATGACGTGGTCGCCCAGCTCGCCGACCAGCACAAGCCGAAGGTCATCGT
GGCCGGCGGCTCGGCCTATCCGCGCGTCATCGATTTCGCCCGCATGCGGCAGATCGCCGACAGCGTCGGCGCCAAGCTCA
TGGTGGACATGGCGCACTTCGCGGGCCTCGTGGCCGGCGGCGCCCACCCGAGCCCGTTCCCGCACGCCCATGTGGTGACC
ACCACCACGCACAAGACCCTGCGCGGCCCGCGCGGCGGCATGATCCTCACGAATGACGAGGAGCTGGCGAAGAAGCTCAA
CTCCGCCATCTTCCCCGGCATCCAGGGTGGCCCGCTCATGCATGTCATCGCCGCCAAGGCGGTGGCCTTCGGCGAAGCGC
TGCGCCCCGAGTTCAAGGTCTATGCCAAGAACGTGGTGGAGAATGCCCGCGCGCTGGCCGAGAACCTGCGCGGCCACGGC
TTCGACATCGTGTCCGACGGCACCGACACGCATCTGATGCTGGTGGACCTGCGTCCCAAGCGCCTCACCGGCAAGATTTC
GGAGAATGCGCTCGGCCGCGCCCACATCACCTGCAACAAGAACGGCATTCCGTTCGACCCCGAGAAGCCGGCGGTCACCT
CGGGCGTGCGCCTCGGCACCCCGGCGGGCACCACCCGCGGCTTCGGCGTGGCCGAGTTCCAGCAGATCGGCGACATGATC
GCCGAAGTGCTGGACGTGCTGTCGCAGAAGGGCGTCGCCGAGGACAGCCTCGTGGAAGAAGCGGTGCGCGGCAAGGTGAA
GTCGCTGCTCGCCCGCTTCCCCATCTACAACTGA

Upstream 100 bases:

>100_bases
CAGGTAGGCTTTGGACTTGGCGGGTGCGCTGTGATAAGCGAGCCCTCCGACCGGCCGCCCCGCGAGGAGCCTGCCGCGCC
GAACGTTTCGGAGACTATCC

Downstream 100 bases:

>100_bases
TCCTGCCCGCTCCGGCGACTGCCGGGGCAGGCCCCGTTTGGCAGCGCGCGGGTCATCGGCCCGCGCGCTTTTCGTCCGAT
CCCGTGCGGCAAGGGACAGG

Product: glycine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MASSAAHSASASAEMNRFFSASLAEVDPEIAAAVSAELGRQREEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYY
GGCQFVDVAENLAIDRAKKLFGCAFANVQPNSGSQANQGVFFTLMQPGDTFLGLNLAAGGHLTHGSPVNMSGKWFNAVAY
GVREDDQRIDYDVVAQLADQHKPKVIVAGGSAYPRVIDFARMRQIADSVGAKLMVDMAHFAGLVAGGAHPSPFPHAHVVT
TTTHKTLRGPRGGMILTNDEELAKKLNSAIFPGIQGGPLMHVIAAKAVAFGEALRPEFKVYAKNVVENARALAENLRGHG
FDIVSDGTDTHLMLVDLRPKRLTGKISENALGRAHITCNKNGIPFDPEKPAVTSGVRLGTPAGTTRGFGVAEFQQIGDMI
AEVLDVLSQKGVAEDSLVEEAVRGKVKSLLARFPIYN

Sequences:

>Translated_437_residues
MASSAAHSASASAEMNRFFSASLAEVDPEIAAAVSAELGRQREEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYY
GGCQFVDVAENLAIDRAKKLFGCAFANVQPNSGSQANQGVFFTLMQPGDTFLGLNLAAGGHLTHGSPVNMSGKWFNAVAY
GVREDDQRIDYDVVAQLADQHKPKVIVAGGSAYPRVIDFARMRQIADSVGAKLMVDMAHFAGLVAGGAHPSPFPHAHVVT
TTTHKTLRGPRGGMILTNDEELAKKLNSAIFPGIQGGPLMHVIAAKAVAFGEALRPEFKVYAKNVVENARALAENLRGHG
FDIVSDGTDTHLMLVDLRPKRLTGKISENALGRAHITCNKNGIPFDPEKPAVTSGVRLGTPAGTTRGFGVAEFQQIGDMI
AEVLDVLSQKGVAEDSLVEEAVRGKVKSLLARFPIYN
>Mature_436_residues
ASSAAHSASASAEMNRFFSASLAEVDPEIAAAVSAELGRQREEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYG
GCQFVDVAENLAIDRAKKLFGCAFANVQPNSGSQANQGVFFTLMQPGDTFLGLNLAAGGHLTHGSPVNMSGKWFNAVAYG
VREDDQRIDYDVVAQLADQHKPKVIVAGGSAYPRVIDFARMRQIADSVGAKLMVDMAHFAGLVAGGAHPSPFPHAHVVTT
TTHKTLRGPRGGMILTNDEELAKKLNSAIFPGIQGGPLMHVIAAKAVAFGEALRPEFKVYAKNVVENARALAENLRGHGF
DIVSDGTDTHLMLVDLRPKRLTGKISENALGRAHITCNKNGIPFDPEKPAVTSGVRLGTPAGTTRGFGVAEFQQIGDMIA
EVLDVLSQKGVAEDSLVEEAVRGKVKSLLARFPIYN

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=435, Percent_Identity=46.2068965517241, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI261862352, Length=435, Percent_Identity=46.2068965517241, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862350, Length=435, Percent_Identity=46.2068965517241, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862348, Length=435, Percent_Identity=46.2068965517241, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862346, Length=435, Percent_Identity=45.5172413793103, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI22547186, Length=487, Percent_Identity=41.6837782340862, Blast_Score=298, Evalue=9e-81,
Organism=Homo sapiens, GI22547189, Length=473, Percent_Identity=39.7463002114165, Blast_Score=265, Evalue=7e-71,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=59.7122302158273, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25144732, Length=436, Percent_Identity=44.2660550458716, Blast_Score=346, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=432, Percent_Identity=44.6759259259259, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=458, Percent_Identity=42.3580786026201, Blast_Score=346, Evalue=4e-96,
Organism=Saccharomyces cerevisiae, GI6323087, Length=433, Percent_Identity=43.4180138568129, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24640005, Length=427, Percent_Identity=45.1990632318501, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI221329721, Length=414, Percent_Identity=46.1352657004831, Blast_Score=352, Evalue=4e-97,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46564; Mature: 46432

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSAAHSASASAEMNRFFSASLAEVDPEIAAAVSAELGRQREEIELIASENIVSRAVLE
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHH
AQGSVLTNKYAEGYPGKRYYGGCQFVDVAENLAIDRAKKLFGCAFANVQPNSGSQANQGV
HCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
FFTLMQPGDTFLGLNLAAGGHLTHGSPVNMSGKWFNAVAYGVREDDQRIDYDVVAQLADQ
EEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHC
HKPKVIVAGGSAYPRVIDFARMRQIADSVGAKLMVDMAHFAGLVAGGAHPSPFPHAHVVT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
TTTHKTLRGPRGGMILTNDEELAKKLNSAIFPGIQGGPLMHVIAAKAVAFGEALRPEFKV
ECCHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHH
YAKNVVENARALAENLRGHGFDIVSDGTDTHLMLVDLRPKRLTGKISENALGRAHITCNK
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCHHCCCCCCCCCCEEEEEECC
NGIPFDPEKPAVTSGVRLGTPAGTTRGFGVAEFQQIGDMIAEVLDVLSQKGVAEDSLVEE
CCCCCCCCCCCHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AVRGKVKSLLARFPIYN
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASSAAHSASASAEMNRFFSASLAEVDPEIAAAVSAELGRQREEIELIASENIVSRAVLE
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHH
AQGSVLTNKYAEGYPGKRYYGGCQFVDVAENLAIDRAKKLFGCAFANVQPNSGSQANQGV
HCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
FFTLMQPGDTFLGLNLAAGGHLTHGSPVNMSGKWFNAVAYGVREDDQRIDYDVVAQLADQ
EEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHC
HKPKVIVAGGSAYPRVIDFARMRQIADSVGAKLMVDMAHFAGLVAGGAHPSPFPHAHVVT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
TTTHKTLRGPRGGMILTNDEELAKKLNSAIFPGIQGGPLMHVIAAKAVAFGEALRPEFKV
ECCHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHH
YAKNVVENARALAENLRGHGFDIVSDGTDTHLMLVDLRPKRLTGKISENALGRAHITCNK
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCHHCCCCCCCCCCEEEEEECC
NGIPFDPEKPAVTSGVRLGTPAGTTRGFGVAEFQQIGDMIAEVLDVLSQKGVAEDSLVEE
CCCCCCCCCCCHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AVRGKVKSLLARFPIYN
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA