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The map label for this gene is mcl1 [H]

Identifier: 158422851

GI number: 158422851

Start: 1376731

End: 1377771

Strand: Reverse

Name: mcl1 [H]

Synonym: AZC_1227

Alternate gene names: 158422851

Gene position: 1377771-1376731 (Counterclockwise)

Preceding gene: 158422854

Following gene: 158422850

Centisome position: 25.66

GC content: 65.51

Gene sequence:

>1041_bases
ATGAAACTGCCGCGCAACTTCTTCAAGCCGCTCGCCATCGGAGCCCCTGCCCCCTATCGGGAACTGCCCGTGCGGCTGGA
GCGCATGATCCATTTCGTGCCGCCGCATCTGGAGAAGATCCGCGCCAAGGTACCGGACCTCGCCCGTCAGGTGGACGTGG
TGCTCGGCAATCTGGAAGACGCCATCCCGATCGACGCCAAGGAGGCCGCGCGCACCGGCTTCATCGAGATGGCGAAGGCG
GTGGACTTCGGCACCACGGGACTCTGGACCCGCATCAACGCGCTGAACAGTCCCTGGGCGCTCGACGACATCACCGAAAT
CGTGGGTGCGGTGGGCGACAAGCTCGACGTCATCATGCTGCCGAAGGTGGATGGCCCGTGGGACATCCACTATCTCGACC
AGTTGCTGGCGCAGCTTGAAGCCCGGCACAAGGTGGAGAAGCCCATCCTCATCCACGCCATCCTGGAGACGGCGGAGGGG
GTAAAGAATGTGGAGGCGATCGCTGCGGCGTCTCCGCGCATGCATGGCATGAGCCTCGGCCCTGCCGACCTTGCGGCGTC
GCGCGCCATGAAGACCACCCGCGTCGGCGGCGGCCATCCGGAATACAAGGTGCTGGCAGACCCGGACGGGGATGCGCCCC
GCACCGGCTATCAGCAGGATCTGTGGCACTACACCGTGGGCAAGATGGTGGATGCCTGCGCCTCGGCCGGCATCAAGCCC
TTCTATGGCCCGTTCGGCGACTTCGCCGACGGCCCCGCGTGCGAGAGCCAGTTCCGCAATGCCTTCCTTCAGGGCTGCGT
GGGCGCATGGTCGCTGCATCCGACGCAGATCGACATCGCCAAGCGCGTCTTCAGCCCCGATCCCGGCGAGGTCGCCTTCG
CCCTCAAGATCCTCGAAGCCATGCCGGACGGCGCCGGCGCGGTGATGATCGACGGCAAGATGCAGGATGATGCCACCTGG
AAACAGGCCAAGGTGATCGTGGACCTGGCCCGCATCGTCGCCGCCAAGGATGCGGAGATGGCGAAAATCTACGGCCTGTG
A

Upstream 100 bases:

>100_bases
CTGCGCTGCACCGCCACATGGACATGCTGCACGGCAAAGTTGCTCTTGCCCCTGCCGGAGCGCCTGTCCATTCTGCGGAA
ACAACGATTGGGGGAAACCA

Downstream 100 bases:

>100_bases
GGCCGGCGCCGGCTCATCCCCAGGGCGTGGGCCGGCGTATCAGGGTTAAGGAGTGGTGAAGATCGCGGCGTTGCGTTATG
TGACGGCGCAGCGACACACG

Product: citrate lyase subunit beta

Products: NA

Alternate protein names: (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; L-malyl-CoA/beta-methylmalyl-CoA lyase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKLPRNFFKPLAIGAPAPYRELPVRLERMIHFVPPHLEKIRAKVPDLARQVDVVLGNLEDAIPIDAKEAARTGFIEMAKA
VDFGTTGLWTRINALNSPWALDDITEIVGAVGDKLDVIMLPKVDGPWDIHYLDQLLAQLEARHKVEKPILIHAILETAEG
VKNVEAIAAASPRMHGMSLGPADLAASRAMKTTRVGGGHPEYKVLADPDGDAPRTGYQQDLWHYTVGKMVDACASAGIKP
FYGPFGDFADGPACESQFRNAFLQGCVGAWSLHPTQIDIAKRVFSPDPGEVAFALKILEAMPDGAGAVMIDGKMQDDATW
KQAKVIVDLARIVAAKDAEMAKIYGL

Sequences:

>Translated_346_residues
MKLPRNFFKPLAIGAPAPYRELPVRLERMIHFVPPHLEKIRAKVPDLARQVDVVLGNLEDAIPIDAKEAARTGFIEMAKA
VDFGTTGLWTRINALNSPWALDDITEIVGAVGDKLDVIMLPKVDGPWDIHYLDQLLAQLEARHKVEKPILIHAILETAEG
VKNVEAIAAASPRMHGMSLGPADLAASRAMKTTRVGGGHPEYKVLADPDGDAPRTGYQQDLWHYTVGKMVDACASAGIKP
FYGPFGDFADGPACESQFRNAFLQGCVGAWSLHPTQIDIAKRVFSPDPGEVAFALKILEAMPDGAGAVMIDGKMQDDATW
KQAKVIVDLARIVAAKDAEMAKIYGL
>Mature_346_residues
MKLPRNFFKPLAIGAPAPYRELPVRLERMIHFVPPHLEKIRAKVPDLARQVDVVLGNLEDAIPIDAKEAARTGFIEMAKA
VDFGTTGLWTRINALNSPWALDDITEIVGAVGDKLDVIMLPKVDGPWDIHYLDQLLAQLEARHKVEKPILIHAILETAEG
VKNVEAIAAASPRMHGMSLGPADLAASRAMKTTRVGGGHPEYKVLADPDGDAPRTGYQQDLWHYTVGKMVDACASAGIKP
FYGPFGDFADGPACESQFRNAFLQGCVGAWSLHPTQIDIAKRVFSPDPGEVAFALKILEAMPDGAGAVMIDGKMQDDATW
KQAKVIVDLARIVAAKDAEMAKIYGL

Specific function: Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to beta-methylmalyl-CoA [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI87081764, Length=295, Percent_Identity=23.728813559322, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.24 [H]

Molecular weight: Translated: 37574; Mature: 37574

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPRNFFKPLAIGAPAPYRELPVRLERMIHFVPPHLEKIRAKVPDLARQVDVVLGNLED
CCCCHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AIPIDAKEAARTGFIEMAKAVDFGTTGLWTRINALNSPWALDDITEIVGAVGDKLDVIML
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEE
PKVDGPWDIHYLDQLLAQLEARHKVEKPILIHAILETAEGVKNVEAIAAASPRMHGMSLG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PADLAASRAMKTTRVGGGHPEYKVLADPDGDAPRTGYQQDLWHYTVGKMVDACASAGIKP
HHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
FYGPFGDFADGPACESQFRNAFLQGCVGAWSLHPTQIDIAKRVFSPDPGEVAFALKILEA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
MPDGAGAVMIDGKMQDDATWKQAKVIVDLARIVAAKDAEMAKIYGL
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MKLPRNFFKPLAIGAPAPYRELPVRLERMIHFVPPHLEKIRAKVPDLARQVDVVLGNLED
CCCCHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AIPIDAKEAARTGFIEMAKAVDFGTTGLWTRINALNSPWALDDITEIVGAVGDKLDVIML
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEE
PKVDGPWDIHYLDQLLAQLEARHKVEKPILIHAILETAEGVKNVEAIAAASPRMHGMSLG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PADLAASRAMKTTRVGGGHPEYKVLADPDGDAPRTGYQQDLWHYTVGKMVDACASAGIKP
HHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
FYGPFGDFADGPACESQFRNAFLQGCVGAWSLHPTQIDIAKRVFSPDPGEVAFALKILEA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
MPDGAGAVMIDGKMQDDATWKQAKVIVDLARIVAAKDAEMAKIYGL
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA