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The map label for this gene is hmp [H]

Identifier: 158422798

GI number: 158422798

Start: 1310813

End: 1311937

Strand: Direct

Name: hmp [H]

Synonym: AZC_1174

Alternate gene names: 158422798

Gene position: 1310813-1311937 (Clockwise)

Preceding gene: 158422797

Following gene: 158422799

Centisome position: 24.41

GC content: 69.51

Gene sequence:

>1125_bases
ATGGACGCGCGCACCATCCGCCCGGACCCTCGCCTCGCATCCCCCGCCGAGGTGCCGCCCACCTGGGATCCGGAGCGTGA
CGACGTCCTCGTTGTGCGTCAGGTGCGGCAGGAAACGGCCGACGTAAAGACCTTCGTCCTCGCGCCCGCCACGCCGGCCC
GGTTCCGCTATCTGCCGGGCCAGTTCATCACGCTCGATCTCGACATCGACGGGGTGCGGCTCAACCGCTGCTACACGCTC
TCCTCCAGCCCCACGCGGCCGGACACTGTGGCCATCACCGTCAAGCGCGTGCCCGGCGGCCCGGTTTCGAACTTCCTCCA
CGATCACCTCCGGGAGGGGAGTGTGCTGCGCGCGGTGGGCCCCATGGGGGATTTCACCTGCGCGCAGCACGAGGCGCCCA
AATATCTCTTCCTCTCTGGCGGCAGCGGCATCACGCCGGTCATGTCCATGGCCCGTGCCCATGCGGACCTTGCCACCGGC
GCGGACATCGCCTTCCTGCACAGCGCCCGCACGCCCGCCGACATCATCTTCCGCGCCGAGCTGGAGCTGATGGCGCGGAC
CACACCCACGTTCCGCTTCGCCGCCATCTGCGAGGCGGATGCGCCGTTTGAGCCCTGGCACGGGCTGCGCGGGCGCCTCT
CGCTCGCCCATCTGGAAGCCGCGGTGCCGGATTTCCGGGCCCGCGAGGTTTTCGTCTGCGGGCCCGCACCTTACATGGCT
GCGGTGCGCGAAATGCTGCGCGCGGCTGGCTTCGACATGGCCCGCCACCATGAGGAAAGCTTCGATTTCGCGACGCTCGC
GACCGCCGAACCGGAGGTCGCCGCCGAGGTGATCGCCGCAGAGGGGGAGACCTCCGCCGCGCGTGTGCATCGCGTGGAGT
TCCGCAAGTCCGGCCGGGTCATCGAGTGCCCGGAAGACATGAACGTACTGGAGGCCGCCCGCCGGGCGGGCATGCGCCTG
CCGTCCTCCTGCACTAAGGGCCTGTGCGGCACCTGCAAGTGCAAGCTGGCCGAGGGCACGGTGGAGATGAAGCACGGCGG
CGGCATCCGCCAGCGGGAGATCGATGCGGGCATGGTGCTGATCTGCTGTGCCCGTCCCACCAGCGATCTCGTGATCGAGC
GCTGA

Upstream 100 bases:

>100_bases
TATGAGCCCGGCCCCTATTCTCCGCACACCGAGATGCTCGTGGAGAAGTTCTGCAACTGGTATGTGGGCCGAATGCGCGC
CCAGGGTGCCCGCTGACGCC

Downstream 100 bases:

>100_bases
CGCCATGGCCCGCCCCCCGACCCCCCACCTTGCCGCCGTGTCCGCGCCCGGCACTTCACCCCTCGGCGGGGAGATGGCGC
AGGTGCGGCGCATCGGCTTC

Product: iron-sulfur cluster-binding protein 2

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MDARTIRPDPRLASPAEVPPTWDPERDDVLVVRQVRQETADVKTFVLAPATPARFRYLPGQFITLDLDIDGVRLNRCYTL
SSSPTRPDTVAITVKRVPGGPVSNFLHDHLREGSVLRAVGPMGDFTCAQHEAPKYLFLSGGSGITPVMSMARAHADLATG
ADIAFLHSARTPADIIFRAELELMARTTPTFRFAAICEADAPFEPWHGLRGRLSLAHLEAAVPDFRAREVFVCGPAPYMA
AVREMLRAAGFDMARHHEESFDFATLATAEPEVAAEVIAAEGETSAARVHRVEFRKSGRVIECPEDMNVLEAARRAGMRL
PSSCTKGLCGTCKCKLAEGTVEMKHGGGIRQREIDAGMVLICCARPTSDLVIER

Sequences:

>Translated_374_residues
MDARTIRPDPRLASPAEVPPTWDPERDDVLVVRQVRQETADVKTFVLAPATPARFRYLPGQFITLDLDIDGVRLNRCYTL
SSSPTRPDTVAITVKRVPGGPVSNFLHDHLREGSVLRAVGPMGDFTCAQHEAPKYLFLSGGSGITPVMSMARAHADLATG
ADIAFLHSARTPADIIFRAELELMARTTPTFRFAAICEADAPFEPWHGLRGRLSLAHLEAAVPDFRAREVFVCGPAPYMA
AVREMLRAAGFDMARHHEESFDFATLATAEPEVAAEVIAAEGETSAARVHRVEFRKSGRVIECPEDMNVLEAARRAGMRL
PSSCTKGLCGTCKCKLAEGTVEMKHGGGIRQREIDAGMVLICCARPTSDLVIER
>Mature_374_residues
MDARTIRPDPRLASPAEVPPTWDPERDDVLVVRQVRQETADVKTFVLAPATPARFRYLPGQFITLDLDIDGVRLNRCYTL
SSSPTRPDTVAITVKRVPGGPVSNFLHDHLREGSVLRAVGPMGDFTCAQHEAPKYLFLSGGSGITPVMSMARAHADLATG
ADIAFLHSARTPADIIFRAELELMARTTPTFRFAAICEADAPFEPWHGLRGRLSLAHLEAAVPDFRAREVFVCGPAPYMA
AVREMLRAAGFDMARHHEESFDFATLATAEPEVAAEVIAAEGETSAARVHRVEFRKSGRVIECPEDMNVLEAARRAGMRL
PSSCTKGLCGTCKCKLAEGTVEMKHGGGIRQREIDAGMVLICCARPTSDLVIER

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta- cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=357, Percent_Identity=31.6526610644258, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787098, Length=346, Percent_Identity=31.2138728323699, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788903, Length=250, Percent_Identity=29.2, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788104, Length=345, Percent_Identity=27.2463768115942, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323552, Length=202, Percent_Identity=25.7425742574257, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322146, Length=252, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40832; Mature: 40832

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARTIRPDPRLASPAEVPPTWDPERDDVLVVRQVRQETADVKTFVLAPATPARFRYLPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHEEECCC
QFITLDLDIDGVRLNRCYTLSSSPTRPDTVAITVKRVPGGPVSNFLHDHLREGSVLRAVG
EEEEEEEEECCEEEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEC
PMGDFTCAQHEAPKYLFLSGGSGITPVMSMARAHADLATGADIAFLHSARTPADIIFRAE
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHEEEEEE
LELMARTTPTFRFAAICEADAPFEPWHGLRGRLSLAHLEAAVPDFRAREVFVCGPAPYMA
EHEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCEEEEECCCHHHH
AVREMLRAAGFDMARHHEESFDFATLATAEPEVAAEVIAAEGETSAARVHRVEFRKSGRV
HHHHHHHHCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCE
IECPEDMNVLEAARRAGMRLPSSCTKGLCGTCKCKLAEGTVEMKHGGGIRQREIDAGMVL
EECCCCCHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECCEEEEECCCCCCEEECCCCEEE
ICCARPTSDLVIER
EEECCCCCCCEEEC
>Mature Secondary Structure
MDARTIRPDPRLASPAEVPPTWDPERDDVLVVRQVRQETADVKTFVLAPATPARFRYLPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHEEECCC
QFITLDLDIDGVRLNRCYTLSSSPTRPDTVAITVKRVPGGPVSNFLHDHLREGSVLRAVG
EEEEEEEEECCEEEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEC
PMGDFTCAQHEAPKYLFLSGGSGITPVMSMARAHADLATGADIAFLHSARTPADIIFRAE
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHEEEEEE
LELMARTTPTFRFAAICEADAPFEPWHGLRGRLSLAHLEAAVPDFRAREVFVCGPAPYMA
EHEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCEEEEECCCHHHH
AVREMLRAAGFDMARHHEESFDFATLATAEPEVAAEVIAAEGETSAARVHRVEFRKSGRV
HHHHHHHHCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCE
IECPEDMNVLEAARRAGMRLPSSCTKGLCGTCKCKLAEGTVEMKHGGGIRQREIDAGMVL
EECCCCCHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECCEEEEECCCCCCEEECCCCEEE
ICCARPTSDLVIER
EEECCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]