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The map label for this gene is mrcA [H]

Identifier: 158422723

GI number: 158422723

Start: 1225542

End: 1227704

Strand: Reverse

Name: mrcA [H]

Synonym: AZC_1099

Alternate gene names: 158422723

Gene position: 1227704-1225542 (Counterclockwise)

Preceding gene: 158422725

Following gene: 158422722

Centisome position: 22.86

GC content: 66.9

Gene sequence:

>2163_bases
GTGAGCGAAGGCGAGCAGAACCCGTTCCGGAAACGGCTGCGCAACGCGCTGCTGGATCTCGATTCCCGCATCGACAGCGG
GATGTACCGGCTGGGCGATCTCACCCGCCGCGCGGTCACGAATTATTCCGCCTTCATGGAGCACTTCTCCCTGAGCGGCA
TCCCGCGCTTCTTCGTCGGGATGGCCTCGGAAGGCTTCACGCTGGGCGCCGCCGGTTTCGTCGTGCTGCTGGCCCTTGCG
CTGCCGGCCTTCCAGGAAACCTCCGACGACTGGCTGAAGCGCACGGAGCTCGCCGTCACCTTCCTCGACCGCTACGGCAA
TCTCGTGGGCGAGCGCGGGGTGAAGCAGAACGACACGGTGCCGCTGGAGGAAATCCCCGACACCCTCATCAAGGCGACGC
TCGCCACCGAGGACCGGCGCTTCTACGAGCATTTCGGCATCGACGTCGCCGGCACCCTGCGTGCCCTCGTCACCAATGCG
CGGGCCGGCGGCGTGCGGCAGGGCGGCTCCTCGCTCTCCCAGCAGCTCGCCAAGAACCTGTTCCTCTCCAATGAGCGGAC
CATCGAACGCAAGATCAAGGAGGCCTTCCTCGCTCTGTGGCTGGAAAGCCGCCTGACCAAGAGCCAGATCCTGAAGCTCT
ATCTGGACCGCGCCTATCTCGGCGGCGGCGCCTATGGCGTGGACGCGGCGGCCCGCTATTATTTCGGCAAATCGGTGCGC
GAGGTGAATCTGGCCGAAAGCGCCATGCTGGCGGGCCTGTTCAAGGCCCCGACCAAATTCGCGCCGCACATCAACCTGCC
CGCCGCCCGCGCCCGCGCCTCCACCGTGCTCGACAATCTGGTGGACGCCGGCTTCATGACCGAGGGCCAGGTGTTCGGCG
CCCGCCGCAATCCGGCGACCCCCATCGACCGGCGCGACGAGGGCGTGCCGGACTATTATCTCGACTATGCCTTCGCCGAA
GTGCGCAAGTTCGTGGACACGCTCCCCAAGAGCGTGACCGAGCGCAATTTCGTGGTGCGCACCGGCCTCGACCCGAACAT
CCAGAAGGTGGCGGAAGCCGCCCTCGACCAGTCCCTGCGGGAATATGGCAAGGACTACAAGGTCACGCAGGGCGCCATCG
TCATCATGGAGCCGAACGGCACCGTGCGCGCCATGGTCGGCGGGCGCGACTATGGCGAGAGCCAGTTCAACCGCGCCACC
GACGCCCTGCGCCAGCCCGGCTCTTCCTTCAAGCCGTTCGTCTATACGACCGCCTTGCTCGGCGGCATGAAGCCCACCTC
CACCGTGGTGGATGCGCCCTATTGCAACGGCAACTGGTGCCCGCAGAATTACGGCCGCAGCTACATGGGCACCATCACGC
TCCAGACCGCGCTCCAGCATTCGCTGAACACGGTGGCGGTGCGCCTCGGCGACAAGTTCGGCCGCACCAACATCGCCGCG
CTCGCCCACAAGATGGGCCTCACCACCAAGCTCGAAATCTCCCCCGCCCTGCCGCTCGGTGCCGCCGAGGTGACGCTCAT
CGACATGGCCACCGCCTATTCGGTGTTCGCCAATGGCGGGCGCAGCGTCTATCCCCACGCCATGGTGGAGATGCGCATCG
GCAATGGCGAGGTGGTCTGGCGGTTCGACCGCGACGCGCCCAAGCCCGTGCAGATCCTGCCGCCGGACATCATCGACGAG
ATCAACCCGATGCTGAACAACGTCATCGAGAACGGCACCGGGCGTCGGGCCCGCCTGCCGGGCATCAAGGTGGCGGGCAA
GACGGGCACCACGAACGCCTATCGCGATGCCTGGTTCATGGCCTTCACCGGCAATTACGTGGGCGGCGTCTGGTTCGGCA
ATGACGACTACCAGTCCATGAACAAGATGACCGGCGGCTCCCTGCCCGCCATGACGTGGCAGAAGGTCATGGCCTATGCC
CATCAGGGCATCGAGCTGAAATACATCCCCGGCATCGGCAACGCCCCGGCGCCCAAGCTCGACCAGCCGCCGGCGCCGCT
GGTGGTGCCGGGCCTTGAATCCACCGAGCGGCCCATCACCCTCTCGCCGAAATCCGCCGCCAACCTCGCGCGCCTCGAAC
GGCTGCTGAAGGAGGCGCCGCCCTTGCCGGCGCGGCCCGCCGGCCCCACCGCTTCCGCCGCGCCGCTCGGCCGGACGAAC
TGA

Upstream 100 bases:

>100_bases
AGCGTACCGCAGCGGCGGGGCGCTTTCCTCCTGCGCCGGTTCGCCTTAGGTTCAGAGCGGCCGCGACGGCGCGGCCTCCA
AGAAGATCGGTTCCCGGCCA

Downstream 100 bases:

>100_bases
AGGCCGGCCCGTGCGTCCGCTCTCCCTCGCCGTCTGCCTCACCGCCGCCGCCGCCCTCGGCCTCGGCGGCACCTGGCTCA
CCGTCACCTCCTCGCGCGGC

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MSEGEQNPFRKRLRNALLDLDSRIDSGMYRLGDLTRRAVTNYSAFMEHFSLSGIPRFFVGMASEGFTLGAAGFVVLLALA
LPAFQETSDDWLKRTELAVTFLDRYGNLVGERGVKQNDTVPLEEIPDTLIKATLATEDRRFYEHFGIDVAGTLRALVTNA
RAGGVRQGGSSLSQQLAKNLFLSNERTIERKIKEAFLALWLESRLTKSQILKLYLDRAYLGGGAYGVDAAARYYFGKSVR
EVNLAESAMLAGLFKAPTKFAPHINLPAARARASTVLDNLVDAGFMTEGQVFGARRNPATPIDRRDEGVPDYYLDYAFAE
VRKFVDTLPKSVTERNFVVRTGLDPNIQKVAEAALDQSLREYGKDYKVTQGAIVIMEPNGTVRAMVGGRDYGESQFNRAT
DALRQPGSSFKPFVYTTALLGGMKPTSTVVDAPYCNGNWCPQNYGRSYMGTITLQTALQHSLNTVAVRLGDKFGRTNIAA
LAHKMGLTTKLEISPALPLGAAEVTLIDMATAYSVFANGGRSVYPHAMVEMRIGNGEVVWRFDRDAPKPVQILPPDIIDE
INPMLNNVIENGTGRRARLPGIKVAGKTGTTNAYRDAWFMAFTGNYVGGVWFGNDDYQSMNKMTGGSLPAMTWQKVMAYA
HQGIELKYIPGIGNAPAPKLDQPPAPLVVPGLESTERPITLSPKSAANLARLERLLKEAPPLPARPAGPTASAAPLGRTN

Sequences:

>Translated_720_residues
MSEGEQNPFRKRLRNALLDLDSRIDSGMYRLGDLTRRAVTNYSAFMEHFSLSGIPRFFVGMASEGFTLGAAGFVVLLALA
LPAFQETSDDWLKRTELAVTFLDRYGNLVGERGVKQNDTVPLEEIPDTLIKATLATEDRRFYEHFGIDVAGTLRALVTNA
RAGGVRQGGSSLSQQLAKNLFLSNERTIERKIKEAFLALWLESRLTKSQILKLYLDRAYLGGGAYGVDAAARYYFGKSVR
EVNLAESAMLAGLFKAPTKFAPHINLPAARARASTVLDNLVDAGFMTEGQVFGARRNPATPIDRRDEGVPDYYLDYAFAE
VRKFVDTLPKSVTERNFVVRTGLDPNIQKVAEAALDQSLREYGKDYKVTQGAIVIMEPNGTVRAMVGGRDYGESQFNRAT
DALRQPGSSFKPFVYTTALLGGMKPTSTVVDAPYCNGNWCPQNYGRSYMGTITLQTALQHSLNTVAVRLGDKFGRTNIAA
LAHKMGLTTKLEISPALPLGAAEVTLIDMATAYSVFANGGRSVYPHAMVEMRIGNGEVVWRFDRDAPKPVQILPPDIIDE
INPMLNNVIENGTGRRARLPGIKVAGKTGTTNAYRDAWFMAFTGNYVGGVWFGNDDYQSMNKMTGGSLPAMTWQKVMAYA
HQGIELKYIPGIGNAPAPKLDQPPAPLVVPGLESTERPITLSPKSAANLARLERLLKEAPPLPARPAGPTASAAPLGRTN
>Mature_719_residues
SEGEQNPFRKRLRNALLDLDSRIDSGMYRLGDLTRRAVTNYSAFMEHFSLSGIPRFFVGMASEGFTLGAAGFVVLLALAL
PAFQETSDDWLKRTELAVTFLDRYGNLVGERGVKQNDTVPLEEIPDTLIKATLATEDRRFYEHFGIDVAGTLRALVTNAR
AGGVRQGGSSLSQQLAKNLFLSNERTIERKIKEAFLALWLESRLTKSQILKLYLDRAYLGGGAYGVDAAARYYFGKSVRE
VNLAESAMLAGLFKAPTKFAPHINLPAARARASTVLDNLVDAGFMTEGQVFGARRNPATPIDRRDEGVPDYYLDYAFAEV
RKFVDTLPKSVTERNFVVRTGLDPNIQKVAEAALDQSLREYGKDYKVTQGAIVIMEPNGTVRAMVGGRDYGESQFNRATD
ALRQPGSSFKPFVYTTALLGGMKPTSTVVDAPYCNGNWCPQNYGRSYMGTITLQTALQHSLNTVAVRLGDKFGRTNIAAL
AHKMGLTTKLEISPALPLGAAEVTLIDMATAYSVFANGGRSVYPHAMVEMRIGNGEVVWRFDRDAPKPVQILPPDIIDEI
NPMLNNVIENGTGRRARLPGIKVAGKTGTTNAYRDAWFMAFTGNYVGGVWFGNDDYQSMNKMTGGSLPAMTWQKVMAYAH
QGIELKYIPGIGNAPAPKLDQPPAPLVVPGLESTERPITLSPKSAANLARLERLLKEAPPLPARPAGPTASAAPLGRTN

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=554, Percent_Identity=32.6714801444043, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788867, Length=516, Percent_Identity=30.8139534883721, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87082258, Length=308, Percent_Identity=36.3636363636364, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI1789601, Length=190, Percent_Identity=36.8421052631579, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 78684; Mature: 78553

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGEQNPFRKRLRNALLDLDSRIDSGMYRLGDLTRRAVTNYSAFMEHFSLSGIPRFFVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MASEGFTLGAAGFVVLLALALPAFQETSDDWLKRTELAVTFLDRYGNLVGERGVKQNDTV
HCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLEEIPDTLIKATLATEDRRFYEHFGIDVAGTLRALVTNARAGGVRQGGSSLSQQLAKNL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
FLSNERTIERKIKEAFLALWLESRLTKSQILKLYLDRAYLGGGAYGVDAAARYYFGKSVR
HCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHH
EVNLAESAMLAGLFKAPTKFAPHINLPAARARASTVLDNLVDAGFMTEGQVFGARRNPAT
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCC
PIDRRDEGVPDYYLDYAFAEVRKFVDTLPKSVTERNFVVRTGLDPNIQKVAEAALDQSLR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
EYGKDYKVTQGAIVIMEPNGTVRAMVGGRDYGESQFNRATDALRQPGSSFKPFVYTTALL
HHCCCCEEECCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GGMKPTSTVVDAPYCNGNWCPQNYGRSYMGTITLQTALQHSLNTVAVRLGDKFGRTNIAA
CCCCCCCCEEECCCCCCCCCCHHHCCCCEEHHHHHHHHHHHHHHEEEECCCCCCCHHHHH
LAHKMGLTTKLEISPALPLGAAEVTLIDMATAYSVFANGGRSVYPHAMVEMRIGNGEVVW
HHHHHCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEE
RFDRDAPKPVQILPPDIIDEINPMLNNVIENGTGRRARLPGIKVAGKTGTTNAYRDAWFM
EECCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCHHCEEEE
AFTGNYVGGVWFGNDDYQSMNKMTGGSLPAMTWQKVMAYAHQGIELKYIPGIGNAPAPKL
EEECCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
DQPPAPLVVPGLESTERPITLSPKSAANLARLERLLKEAPPLPARPAGPTASAAPLGRTN
CCCCCCEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEGEQNPFRKRLRNALLDLDSRIDSGMYRLGDLTRRAVTNYSAFMEHFSLSGIPRFFVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MASEGFTLGAAGFVVLLALALPAFQETSDDWLKRTELAVTFLDRYGNLVGERGVKQNDTV
HCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLEEIPDTLIKATLATEDRRFYEHFGIDVAGTLRALVTNARAGGVRQGGSSLSQQLAKNL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
FLSNERTIERKIKEAFLALWLESRLTKSQILKLYLDRAYLGGGAYGVDAAARYYFGKSVR
HCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHH
EVNLAESAMLAGLFKAPTKFAPHINLPAARARASTVLDNLVDAGFMTEGQVFGARRNPAT
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCC
PIDRRDEGVPDYYLDYAFAEVRKFVDTLPKSVTERNFVVRTGLDPNIQKVAEAALDQSLR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
EYGKDYKVTQGAIVIMEPNGTVRAMVGGRDYGESQFNRATDALRQPGSSFKPFVYTTALL
HHCCCCEEECCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GGMKPTSTVVDAPYCNGNWCPQNYGRSYMGTITLQTALQHSLNTVAVRLGDKFGRTNIAA
CCCCCCCCEEECCCCCCCCCCHHHCCCCEEHHHHHHHHHHHHHHEEEECCCCCCCHHHHH
LAHKMGLTTKLEISPALPLGAAEVTLIDMATAYSVFANGGRSVYPHAMVEMRIGNGEVVW
HHHHHCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEE
RFDRDAPKPVQILPPDIIDEINPMLNNVIENGTGRRARLPGIKVAGKTGTTNAYRDAWFM
EECCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCHHCEEEE
AFTGNYVGGVWFGNDDYQSMNKMTGGSLPAMTWQKVMAYAHQGIELKYIPGIGNAPAPKL
EEECCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
DQPPAPLVVPGLESTERPITLSPKSAANLARLERLLKEAPPLPARPAGPTASAAPLGRTN
CCCCCCEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]