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The map label for this gene is nemA [H]

Identifier: 158422690

GI number: 158422690

Start: 1194535

End: 1195623

Strand: Direct

Name: nemA [H]

Synonym: AZC_1066

Alternate gene names: 158422690

Gene position: 1194535-1195623 (Clockwise)

Preceding gene: 158422689

Following gene: 158422691

Centisome position: 22.25

GC content: 66.48

Gene sequence:

>1089_bases
ATGACAGCGTCTCTCTTCGAGCCCTACCGCCTTGGCGACATCACGCTCTCCAACCGCATTGTCATGGCGCCGCTGACGCG
CAACCGCGCCGCGCCCGGCAACATCGCCACGGATCTCACCGCCCAATATTACGCCCAGCGCGCCAGCGCCGGCCTCATCA
TCTCCGAGGCGAGCCAGATTTCGCCGGAGGGCCAAGGCTATCAGGACACCCCCGGCATCCATTCCCCCGAGCAGATCGCC
GGCTGGAAGAAGGTGACGAAGGCCGTGCATGACGCCGGCGGCCGCATCTTCCTGCAGGCCTGGCACGTGGGCCGCATCTC
CCACACCTCGCTCCAGCCCGGCGGCAAGGCTCCGGTGGCGCCCTCCGCCATCCGTGCCAACGGCAAGACCTTCGTGAACA
ACGCCTTCACCGACGTCTCCGAGCCGCGCGCGCTTGAGTTGTCCGAGCTTCCCGGCATCGTCGAGGACTATCGCAAGGCT
GCCGCCAACGCCATCGAGGCCGGCTTTGACGGCATCGAGATCCACGCCGCGAACGGCTATCTCCTCGACCAGTTCCTCCG
CGACGGCGCCAACAAGCGCACGGACGCCTATGGCGGCTCCATCGAGAACCGCGCCCGGCTCCTGCTGGAAGTGATGGAGG
CGGTCATCAAGGAGATCGGCGCCAACCGCGTCGGCATCCGTCTCTCGCCCGTAACCCCCGCCAACGACCTGACGGACAGC
AATCCGCAGGCCCTGTTCAATTACGTGGTCGAGAAGCTGAACGGGCTGAAGCCGGTCTATATCCACGTGGTCGAGGGCGC
CACGGGCGGCCCGCGCGATGTGGCGCCCGACTTTGATTTCGAGGCCCTGCACAAGCGCTTCGATGGCACCTGGATGACCA
ACAACGGCTATGACAAGGCTCTGGCCGAGACGGTGCTGGCGGAAGGCAAGGCGGACCTCATCGCCTTCGGCCGTCCCTTC
ATCTCCAATCCCGACCTTGTGGAGCGCCTCAAGCGCGGCGCGCCGCTGAACGAGCTGAACCGCGACACGCTCTATGGCGG
CGGGGCGACCGGCTACACCGACTATCCGACCCTCGGCACGGCTGCGTGA

Upstream 100 bases:

>100_bases
GCACTCTATGTGTTGTGCATTGCAACCCACCTGTCCGGCCTGTTGCGCGACGCCCACTCTGCTGCCTGCCGCCGACACGC
CGATCCCAGAAAGGTCTCCG

Downstream 100 bases:

>100_bases
GCGCAGACGGCGATACGGGGACGGTCAGCGTCCATCCGGTCTCCCGGCTCGACCTTCGGGTCGAGCCGGGGATCTGGACC
GAGGCGGAGCGCCATCGCTC

Product: morphinone reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTASLFEPYRLGDITLSNRIVMAPLTRNRAAPGNIATDLTAQYYAQRASAGLIISEASQISPEGQGYQDTPGIHSPEQIA
GWKKVTKAVHDAGGRIFLQAWHVGRISHTSLQPGGKAPVAPSAIRANGKTFVNNAFTDVSEPRALELSELPGIVEDYRKA
AANAIEAGFDGIEIHAANGYLLDQFLRDGANKRTDAYGGSIENRARLLLEVMEAVIKEIGANRVGIRLSPVTPANDLTDS
NPQALFNYVVEKLNGLKPVYIHVVEGATGGPRDVAPDFDFEALHKRFDGTWMTNNGYDKALAETVLAEGKADLIAFGRPF
ISNPDLVERLKRGAPLNELNRDTLYGGGATGYTDYPTLGTAA

Sequences:

>Translated_362_residues
MTASLFEPYRLGDITLSNRIVMAPLTRNRAAPGNIATDLTAQYYAQRASAGLIISEASQISPEGQGYQDTPGIHSPEQIA
GWKKVTKAVHDAGGRIFLQAWHVGRISHTSLQPGGKAPVAPSAIRANGKTFVNNAFTDVSEPRALELSELPGIVEDYRKA
AANAIEAGFDGIEIHAANGYLLDQFLRDGANKRTDAYGGSIENRARLLLEVMEAVIKEIGANRVGIRLSPVTPANDLTDS
NPQALFNYVVEKLNGLKPVYIHVVEGATGGPRDVAPDFDFEALHKRFDGTWMTNNGYDKALAETVLAEGKADLIAFGRPF
ISNPDLVERLKRGAPLNELNRDTLYGGGATGYTDYPTLGTAA
>Mature_361_residues
TASLFEPYRLGDITLSNRIVMAPLTRNRAAPGNIATDLTAQYYAQRASAGLIISEASQISPEGQGYQDTPGIHSPEQIAG
WKKVTKAVHDAGGRIFLQAWHVGRISHTSLQPGGKAPVAPSAIRANGKTFVNNAFTDVSEPRALELSELPGIVEDYRKAA
ANAIEAGFDGIEIHAANGYLLDQFLRDGANKRTDAYGGSIENRARLLLEVMEAVIKEIGANRVGIRLSPVTPANDLTDSN
PQALFNYVVEKLNGLKPVYIHVVEGATGGPRDVAPDFDFEALHKRFDGTWMTNNGYDKALAETVLAEGKADLIAFGRPFI
SNPDLVERLKRGAPLNELNRDTLYGGGATGYTDYPTLGTAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=364, Percent_Identity=52.7472527472528, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=29.8550724637681, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17559802, Length=299, Percent_Identity=28.7625418060201, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=277, Percent_Identity=28.8808664259928, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17565138, Length=312, Percent_Identity=27.2435897435897, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=277, Percent_Identity=27.0758122743682, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=251, Percent_Identity=33.0677290836653, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=80, Percent_Identity=45, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17566914, Length=248, Percent_Identity=28.6290322580645, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=380, Percent_Identity=40.2631578947368, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=380, Percent_Identity=39.4736842105263, Blast_Score=227, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38945; Mature: 38814

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLFEPYRLGDITLSNRIVMAPLTRNRAAPGNIATDLTAQYYAQRASAGLIISEASQI
CCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECHHHC
SPEGQGYQDTPGIHSPEQIAGWKKVTKAVHDAGGRIFLQAWHVGRISHTSLQPGGKAPVA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCCCCCCCC
PSAIRANGKTFVNNAFTDVSEPRALELSELPGIVEDYRKAAANAIEAGFDGIEIHAANGY
CHHHHCCCCHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCH
LLDQFLRDGANKRTDAYGGSIENRARLLLEVMEAVIKEIGANRVGIRLSPVTPANDLTDS
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
NPQALFNYVVEKLNGLKPVYIHVVEGATGGPRDVAPDFDFEALHKRFDGTWMTNNGYDKA
CHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEECCCCHHHH
LAETVLAEGKADLIAFGRPFISNPDLVERLKRGAPLNELNRDTLYGGGATGYTDYPTLGT
HHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
AA
CC
>Mature Secondary Structure 
TASLFEPYRLGDITLSNRIVMAPLTRNRAAPGNIATDLTAQYYAQRASAGLIISEASQI
CCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECHHHC
SPEGQGYQDTPGIHSPEQIAGWKKVTKAVHDAGGRIFLQAWHVGRISHTSLQPGGKAPVA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCCCCCCCC
PSAIRANGKTFVNNAFTDVSEPRALELSELPGIVEDYRKAAANAIEAGFDGIEIHAANGY
CHHHHCCCCHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCH
LLDQFLRDGANKRTDAYGGSIENRARLLLEVMEAVIKEIGANRVGIRLSPVTPANDLTDS
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
NPQALFNYVVEKLNGLKPVYIHVVEGATGGPRDVAPDFDFEALHKRFDGTWMTNNGYDKA
CHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEECCCCHHHH
LAETVLAEGKADLIAFGRPFISNPDLVERLKRGAPLNELNRDTLYGGGATGYTDYPTLGT
HHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]