Click here to switch to the map view.

The map label for this gene is nifA

Identifier: 158422673

GI number: 158422673

Start: 1178139

End: 1179986

Strand: Reverse

Name: nifA

Synonym: AZC_1049

Alternate gene names: 158422673

Gene position: 1179986-1178139 (Counterclockwise)

Preceding gene: 158422674

Following gene: 158422672

Centisome position: 21.97

GC content: 67.48

Gene sequence:

>1848_bases
ATGCCAATGACCGACGCCTTCCAGGTCCGCGTACCTCGGGTTTCGTCGAGCACCGCCGGAGACATCGCCGCGTCATCCAT
CACCACGCGGGGCGCGCTGCCGCGCCCGGGAGGGATGCCTGTGTCCATGTCGCGGGGGACCTCGCCCGAGGTGGCACTCA
TCGGGGTCTATGAGATATCGAAGATCCTGACGGCGCCCCGGCGCCTCGAAGTCACGCTCGCCAATGTGGTGAACGTGCTC
TCCTCCATGCTGCAGATGCGGCATGGCATGATCTGCATCCTCGACAGCGAGGGCGATCCCGACATGGTGGCCACCACCGG
CTGGACGCCTGAGATGGCGGGCCAGATCCGCGCGCATGTGCCCCAGAAGGCCATCGACCAGATCGTCGCCACGCAGATGC
CGCTGGTGGTGCAGGACGTGACGGCCGATCCGCTCTTCGCCGGTCACGAGGATCTGTTCGGCCCGCCTGAGGAGGCCACC
GTCTCCTTCATCGGCGTGCCGATCAAGGCCGACCACCATGTGATGGGCACCCTCTCCATCGACCGCATCTGGGACGGCAC
CGCCCGTTTCCGCTTCGACGAGGACGTGCGCTTCCTCACCATGGTGGCCAATCTCGTCGGCCAGACCGTGCGCCTGCACA
AGCTGGTGGCGAGCGACCGCGACCGGCTGATCGCCCAGACGCACCGCCTCGAAAAGGCGCTGCGGGAAGAAAAATCCGGG
GCCGAGCCGGAGGTGGCCGAGGCCGCCAACGGATCCGCCATGGGCATCGTGGGCGATAGCCCGCTGGTGAAACGCCTGAT
CGCGACCGCGCAAGTGGTCGCCCGCTCAAACTCCACCGTGCTGCTGCGCGGGGAGAGCGGCACCGGCAAGGAGTTGTTCG
CCCGTGCCATCCACGAACTGTCGCCCCGCAAGGGCAAGCCCTTCGTGAAGGTGAACTGCGCCGCCCTCCCGGAATCGGTG
CTGGAATCGGAACTGTTCGGCCATGAGAAGGGCGCCTTCACCGGTGCGCTGAACATGCGCCAGGGCCGCTTCGAGCTGGC
GCACGGCGGCACGCTCTTCCTTGACGAGATCGGCGAGATCACCCCCGCTTTCCAGGCCAAGCTGCTGCGCGTGCTGCAGG
AAGGCGAGTTCGAGCGGGTCGGCGGCAATCGCACGCTGAAGGTGGATGTGCGGCTCGTGTGCGCCACCAACAAGAATCTG
GAAGAGGCGGTCTCCAAGGGCGAGTTCCGGGCCGATCTCTACTACCGCATCCATGTGGTGCCGCTGATCCTGCCGCCGCT
GCGCGAACGGCCGGGCGACATTCCCAAGCTCGCGAAGAACTTCCTCGACCGCTTCAACAAGGAAAACAAGCTCCACATGA
TGCTCTCGGCGCCGGCCATCGACGTGCTGCGGCGCTGCTATTTCCCGGGCAACGTGCGCGAGCTGGAGAACTGTATCCGG
CGGACGGCAACGCTCGCCCACGATGCCGTCATCACCCCCCATGACTTCGCCTGCGACAGCGGCCAGTGCCTCTCGGCCAT
GCTCTGGAAGGGCTCGGCCCCGAAGCCTGTGATGCCGCACGTGCCGCCGGCGCCCACGCCGCTGACTCCGCTCTCCCCTG
CTCCGCTCGCGACCGCAGCGCCCGCTGCGGCGAGCCCGGCGCCGGCGGCCGACAGCCTGCCGGTCACTTGCCCCGGCACC
GAGGCCTGTCCCGCGGTGCCCCCCCGCCAGAGCGAAAAGGAGCAGTTGCTCCAGGCCATGGAGCGCTCCGGCTGGGTGCA
GGCGAAGGCCGCGCGCCTCCTCAACCTCACGCCGCGCCAGGTGGGTTATGCGCTGCGCAAATATGACATCGACATCAAGC
GCTTCTGA

Upstream 100 bases:

>100_bases
TTATAACAATTCCATTAAATCGACGGCCCGGCACACGAGGCCGCTTCAGGGAACACCGACAGGAGGCTGATCCCTCGCAG
CCGCTTTTGCGGCAGGGAGC

Downstream 100 bases:

>100_bases
ACCACGGGGCGGAGCTTGCTCCGCCCTGTCCCGTCCGGCCGCCGATCGCGGCGCCCGCGGGAAAGCGCCGGGCCGTTTTA
TGGGAGCCCCCCTCCCATGA

Product: Nif-specific regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MPMTDAFQVRVPRVSSSTAGDIAASSITTRGALPRPGGMPVSMSRGTSPEVALIGVYEISKILTAPRRLEVTLANVVNVL
SSMLQMRHGMICILDSEGDPDMVATTGWTPEMAGQIRAHVPQKAIDQIVATQMPLVVQDVTADPLFAGHEDLFGPPEEAT
VSFIGVPIKADHHVMGTLSIDRIWDGTARFRFDEDVRFLTMVANLVGQTVRLHKLVASDRDRLIAQTHRLEKALREEKSG
AEPEVAEAANGSAMGIVGDSPLVKRLIATAQVVARSNSTVLLRGESGTGKELFARAIHELSPRKGKPFVKVNCAALPESV
LESELFGHEKGAFTGALNMRQGRFELAHGGTLFLDEIGEITPAFQAKLLRVLQEGEFERVGGNRTLKVDVRLVCATNKNL
EEAVSKGEFRADLYYRIHVVPLILPPLRERPGDIPKLAKNFLDRFNKENKLHMMLSAPAIDVLRRCYFPGNVRELENCIR
RTATLAHDAVITPHDFACDSGQCLSAMLWKGSAPKPVMPHVPPAPTPLTPLSPAPLATAAPAAASPAPAADSLPVTCPGT
EACPAVPPRQSEKEQLLQAMERSGWVQAKAARLLNLTPRQVGYALRKYDIDIKRF

Sequences:

>Translated_615_residues
MPMTDAFQVRVPRVSSSTAGDIAASSITTRGALPRPGGMPVSMSRGTSPEVALIGVYEISKILTAPRRLEVTLANVVNVL
SSMLQMRHGMICILDSEGDPDMVATTGWTPEMAGQIRAHVPQKAIDQIVATQMPLVVQDVTADPLFAGHEDLFGPPEEAT
VSFIGVPIKADHHVMGTLSIDRIWDGTARFRFDEDVRFLTMVANLVGQTVRLHKLVASDRDRLIAQTHRLEKALREEKSG
AEPEVAEAANGSAMGIVGDSPLVKRLIATAQVVARSNSTVLLRGESGTGKELFARAIHELSPRKGKPFVKVNCAALPESV
LESELFGHEKGAFTGALNMRQGRFELAHGGTLFLDEIGEITPAFQAKLLRVLQEGEFERVGGNRTLKVDVRLVCATNKNL
EEAVSKGEFRADLYYRIHVVPLILPPLRERPGDIPKLAKNFLDRFNKENKLHMMLSAPAIDVLRRCYFPGNVRELENCIR
RTATLAHDAVITPHDFACDSGQCLSAMLWKGSAPKPVMPHVPPAPTPLTPLSPAPLATAAPAAASPAPAADSLPVTCPGT
EACPAVPPRQSEKEQLLQAMERSGWVQAKAARLLNLTPRQVGYALRKYDIDIKRF
>Mature_614_residues
PMTDAFQVRVPRVSSSTAGDIAASSITTRGALPRPGGMPVSMSRGTSPEVALIGVYEISKILTAPRRLEVTLANVVNVLS
SMLQMRHGMICILDSEGDPDMVATTGWTPEMAGQIRAHVPQKAIDQIVATQMPLVVQDVTADPLFAGHEDLFGPPEEATV
SFIGVPIKADHHVMGTLSIDRIWDGTARFRFDEDVRFLTMVANLVGQTVRLHKLVASDRDRLIAQTHRLEKALREEKSGA
EPEVAEAANGSAMGIVGDSPLVKRLIATAQVVARSNSTVLLRGESGTGKELFARAIHELSPRKGKPFVKVNCAALPESVL
ESELFGHEKGAFTGALNMRQGRFELAHGGTLFLDEIGEITPAFQAKLLRVLQEGEFERVGGNRTLKVDVRLVCATNKNLE
EAVSKGEFRADLYYRIHVVPLILPPLRERPGDIPKLAKNFLDRFNKENKLHMMLSAPAIDVLRRCYFPGNVRELENCIRR
TATLAHDAVITPHDFACDSGQCLSAMLWKGSAPKPVMPHVPPAPTPLTPLSPAPLATAAPAAASPAPAADSLPVTCPGTE
ACPAVPPRQSEKEQLLQAMERSGWVQAKAARLLNLTPRQVGYALRKYDIDIKRF

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI87082117, Length=377, Percent_Identity=41.3793103448276, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1788550, Length=259, Percent_Identity=51.3513513513513, Blast_Score=263, Evalue=4e-71,
Organism=Escherichia coli, GI1790437, Length=353, Percent_Identity=42.7762039660057, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=56.0344827586207, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1789087, Length=299, Percent_Identity=46.4882943143813, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1790299, Length=258, Percent_Identity=48.062015503876, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788905, Length=239, Percent_Identity=50.2092050209205, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI1787583, Length=242, Percent_Identity=47.9338842975207, Blast_Score=216, Evalue=5e-57,
Organism=Escherichia coli, GI1786524, Length=296, Percent_Identity=45.945945945946, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1789233, Length=226, Percent_Identity=46.0176991150442, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87081872, Length=358, Percent_Identity=37.4301675977654, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87081858, Length=238, Percent_Identity=35.7142857142857, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789828, Length=252, Percent_Identity=38.8888888888889, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFA_AZOC5 (P09133)

Other databases:

- EMBL:   X14716
- EMBL:   X14716
- EMBL:   X08014
- EMBL:   AP009384
- PIR:   S06977
- RefSeq:   YP_001523965.1
- ProteinModelPortal:   P09133
- SMR:   P09133
- GeneID:   5687926
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1049
- HOGENOM:   HBG740884
- OMA:   IGIYEIS
- ProtClustDB:   CLSK2328952
- BioCyc:   ACAU438753:AZC_1049-MONOMER
- GO:   GO:0005622
- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00065
- TIGRFAMs:   TIGR01817

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 66795; Mature: 66664

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMTDAFQVRVPRVSSSTAGDIAASSITTRGALPRPGGMPVSMSRGTSPEVALIGVYEIS
CCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEHHHHH
KILTAPRRLEVTLANVVNVLSSMLQMRHGMICILDSEGDPDMVATTGWTPEMAGQIRAHV
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCHHHHHHHHHCC
PQKAIDQIVATQMPLVVQDVTADPLFAGHEDLFGPPEEATVSFIGVPIKADHHVMGTLSI
CHHHHHHHHHHCCCEEEECCCCCCCCCCCHHCCCCCCCCEEEEEEEEECCCCEEEEEEEH
DRIWDGTARFRFDEDVRFLTMVANLVGQTVRLHKLVASDRDRLIAQTHRLEKALREEKSG
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
AEPEVAEAANGSAMGIVGDSPLVKRLIATAQVVARSNSTVLLRGESGTGKELFARAIHEL
CCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHC
SPRKGKPFVKVNCAALPESVLESELFGHEKGAFTGALNMRQGRFELAHGGTLFLDEIGEI
CCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCEEEEHHHHCC
TPAFQAKLLRVLQEGEFERVGGNRTLKVDVRLVCATNKNLEEAVSKGEFRADLYYRIHVV
CHHHHHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCCHHHHHHCCCCEEEEEEEEEEE
PLILPPLRERPGDIPKLAKNFLDRFNKENKLHMMLSAPAIDVLRRCYFPGNVRELENCIR
EEECCHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RTATLAHDAVITPHDFACDSGQCLSAMLWKGSAPKPVMPHVPPAPTPLTPLSPAPLATAA
HHHHHHHCCEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAAASPAPAADSLPVTCPGTEACPAVPPRQSEKEQLLQAMERSGWVQAKAARLLNLTPRQ
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHH
VGYALRKYDIDIKRF
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
PMTDAFQVRVPRVSSSTAGDIAASSITTRGALPRPGGMPVSMSRGTSPEVALIGVYEIS
CCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEHHHHH
KILTAPRRLEVTLANVVNVLSSMLQMRHGMICILDSEGDPDMVATTGWTPEMAGQIRAHV
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCHHHHHHHHHCC
PQKAIDQIVATQMPLVVQDVTADPLFAGHEDLFGPPEEATVSFIGVPIKADHHVMGTLSI
CHHHHHHHHHHCCCEEEECCCCCCCCCCCHHCCCCCCCCEEEEEEEEECCCCEEEEEEEH
DRIWDGTARFRFDEDVRFLTMVANLVGQTVRLHKLVASDRDRLIAQTHRLEKALREEKSG
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
AEPEVAEAANGSAMGIVGDSPLVKRLIATAQVVARSNSTVLLRGESGTGKELFARAIHEL
CCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHC
SPRKGKPFVKVNCAALPESVLESELFGHEKGAFTGALNMRQGRFELAHGGTLFLDEIGEI
CCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCEEEEHHHHCC
TPAFQAKLLRVLQEGEFERVGGNRTLKVDVRLVCATNKNLEEAVSKGEFRADLYYRIHVV
CHHHHHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCCHHHHHHCCCCEEEEEEEEEEE
PLILPPLRERPGDIPKLAKNFLDRFNKENKLHMMLSAPAIDVLRRCYFPGNVRELENCIR
EEECCHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RTATLAHDAVITPHDFACDSGQCLSAMLWKGSAPKPVMPHVPPAPTPLTPLSPAPLATAA
HHHHHHHCCEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAAASPAPAADSLPVTCPGTEACPAVPPRQSEKEQLLQAMERSGWVQAKAARLLNLTPRQ
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHH
VGYALRKYDIDIKRF
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2664425; 3186446