BacMap

Definition	Azorhizobium caulinodans ORS 571, complete genome.
Accession	NC_009937
Length	5,369,772

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The map label for this gene is ydfG [H]

Identifier: 158422560

GI number: 158422560

Start: 1047800

End: 1048564

Strand: Direct

Name: ydfG [H]

Synonym: AZC_0936

Alternate gene names: 158422560

Gene position: 1047800-1048564 (Clockwise)

Preceding gene: 158422558

Following gene: 158422561

Centisome position: 19.51

GC content: 67.45

Gene sequence:

>765_bases
ATGTCCGCCCTTGATCCGAAGACGCTGACCGTTGTCGTGACCGGAGCCACCTCGGGCATCGGGGCCGCGACCGTGCGGCG
CTTTGCCTCCGCGGGGTCCAAGGTCGTGGCCATGGGCCGCCGAGAGGACCGCCTCCAGGCGCTCGCCCAGGAGTTCCCGA
ATCTCGTCCTGCCGGTGGTCCTCGACGTGCGCGACTACGCTGCCACGGAGGCCGCCTTCGCGGCGCTCCCCGACGCGTTC
AAGGGCTACAACGTGCTGTGCGCCAATGCCGGCCTCGCCCTCGGCCTCGAGCCCGCCCAGGAGGCGAAGATCGAGGACTG
GCAGACCATGATCGACACCAACATCACCGGCCTTGCCTATACGGTGCGCGCCGGCCTGCCGGGCATGGTGGCCCGCAAGG
AAGGCCATGTGGTGTTCACCGGTTCGGTGGCCGGCGACTATCCCTATCCGGGCGGCAACGTCTATGGCGGCACCAAGGCC
TTCGTGAAGCAGTTCGCGCTGAACGTCTGGGCCGATGTGGTGGGCACCGGCGTGCGCGTCACCAATATCGAACCCGGCCT
CACCGAAACGGAATTCTCCGTTGTGCGTTTCCGCGGGGACGAGGCGAAGGCGGACAAGGTCTATGAGGGCACCGCCCCCA
TGACCGGCGAGGACATTGCCGAGCAGGTCTTCTTCGCCGCCACCCTGCCGCGCCATGTGGTGGTGCTCCGCATCCAGGCC
ATGGCCAACCAGCAGAGCTTCAGCCCCTTCTCCATCAAGCGCTGA

Upstream 100 bases:

>100_bases
CCCGCTTCTCGCGCCAGCGCGCGGTGGCGGCATGGACGGATGTTGCACGGCGCGGTATGTCCCCCGCACGCCATCCGCCT
TTCCCGTCAGGAGAAAATCC

Downstream 100 bases:

>100_bases
CGGCAGCCGCCGGGCGCAGGGAGCGCGCCATGAACCTGCCCAACCTCATCACGATCGGCCGGCTCATCATCGTGCCCGTG
GTGATCTGGGCCATCTCCGC

Product: short-chain dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSALDPKTLTVVVTGATSGIGAATVRRFASAGSKVVAMGRREDRLQALAQEFPNLVLPVVLDVRDYAATEAAFAALPDAF
KGYNVLCANAGLALGLEPAQEAKIEDWQTMIDTNITGLAYTVRAGLPGMVARKEGHVVFTGSVAGDYPYPGGNVYGGTKA
FVKQFALNVWADVVGTGVRVTNIEPGLTETEFSVVRFRGDEAKADKVYEGTAPMTGEDIAEQVFFAATLPRHVVVLRIQA
MANQQSFSPFSIKR

Sequences:

>Translated_254_residues
MSALDPKTLTVVVTGATSGIGAATVRRFASAGSKVVAMGRREDRLQALAQEFPNLVLPVVLDVRDYAATEAAFAALPDAF
KGYNVLCANAGLALGLEPAQEAKIEDWQTMIDTNITGLAYTVRAGLPGMVARKEGHVVFTGSVAGDYPYPGGNVYGGTKA
FVKQFALNVWADVVGTGVRVTNIEPGLTETEFSVVRFRGDEAKADKVYEGTAPMTGEDIAEQVFFAATLPRHVVVLRIQA
MANQQSFSPFSIKR
>Mature_253_residues
SALDPKTLTVVVTGATSGIGAATVRRFASAGSKVVAMGRREDRLQALAQEFPNLVLPVVLDVRDYAATEAAFAALPDAFK
GYNVLCANAGLALGLEPAQEAKIEDWQTMIDTNITGLAYTVRAGLPGMVARKEGHVVFTGSVAGDYPYPGGNVYGGTKAF
VKQFALNVWADVVGTGVRVTNIEPGLTETEFSVVRFRGDEAKADKVYEGTAPMTGEDIAEQVFFAATLPRHVVVLRIQAM
ANQQSFSPFSIKR

Specific function: Catalyzes the NADP-dependent oxidation of L-allo- threonine and L-serine [H]

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI109715829, Length=245, Percent_Identity=28.1632653061224, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI20149619, Length=254, Percent_Identity=27.9527559055118, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI157739938, Length=249, Percent_Identity=24.0963855421687, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7705855, Length=194, Percent_Identity=27.8350515463918, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI19923817, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI226371731, Length=189, Percent_Identity=26.984126984127, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI7657478, Length=191, Percent_Identity=30.8900523560209, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI32483357, Length=213, Percent_Identity=28.169014084507, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI40548397, Length=185, Percent_Identity=30.8108108108108, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI214010158, Length=185, Percent_Identity=30.8108108108108, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI214010156, Length=185, Percent_Identity=30.8108108108108, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI22758144, Length=185, Percent_Identity=30.8108108108108, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI223718074, Length=192, Percent_Identity=32.2916666666667, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787820, Length=247, Percent_Identity=51.8218623481781, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI87082100, Length=188, Percent_Identity=31.9148936170213, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787335, Length=219, Percent_Identity=27.3972602739726, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786812, Length=247, Percent_Identity=27.9352226720648, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=244, Percent_Identity=28.2786885245902, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=214, Percent_Identity=28.5046728971963, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=231, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=232, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=192, Percent_Identity=28.125, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=195, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538480, Length=193, Percent_Identity=25.9067357512953, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=190, Percent_Identity=26.8421052631579, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=192, Percent_Identity=26.5625, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=210, Percent_Identity=27.6190476190476, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=205, Percent_Identity=24.8780487804878, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=253, Percent_Identity=35.9683794466403, Blast_Score=155, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6322227, Length=193, Percent_Identity=28.4974093264249, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322067, Length=189, Percent_Identity=32.8042328042328, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=189, Percent_Identity=26.984126984127, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI116007236, Length=238, Percent_Identity=32.7731092436975, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI116007238, Length=160, Percent_Identity=36.875, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571387, Length=245, Percent_Identity=31.0204081632653, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21358495, Length=224, Percent_Identity=27.6785714285714, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24641232, Length=240, Percent_Identity=30.8333333333333, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364494, Length=248, Percent_Identity=29.0322580645161, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648944, Length=228, Percent_Identity=29.8245614035088, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI116007234, Length=239, Percent_Identity=30.9623430962343, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640442, Length=236, Percent_Identity=28.3898305084746, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24641388, Length=195, Percent_Identity=31.7948717948718, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24663911, Length=255, Percent_Identity=29.8039215686275, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24643142, Length=183, Percent_Identity=30.0546448087432, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI23397609, Length=230, Percent_Identity=31.304347826087, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24644339, Length=186, Percent_Identity=30.1075268817204, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648693, Length=233, Percent_Identity=28.755364806867, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355889, Length=233, Percent_Identity=28.755364806867, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357041, Length=192, Percent_Identity=30.2083333333333, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24584748, Length=214, Percent_Identity=27.1028037383178, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI161076933, Length=214, Percent_Identity=27.1028037383178, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571526, Length=199, Percent_Identity=29.6482412060301, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI116007232, Length=141, Percent_Identity=34.0425531914894, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21355319, Length=221, Percent_Identity=25.3393665158371, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27048; Mature: 26917

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALDPKTLTVVVTGATSGIGAATVRRFASAGSKVVAMGRREDRLQALAQEFPNLVLPVV
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCEEEHHH
LDVRDYAATEAAFAALPDAFKGYNVLCANAGLALGLEPAQEAKIEDWQTMIDTNITGLAY
EEHHHHHHHHHHHHHCCHHHCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCEEE
TVRAGLPGMVARKEGHVVFTGSVAGDYPYPGGNVYGGTKAFVKQFALNVWADVVGTGVRV
EEECCCCCCEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
TNIEPGLTETEFSVVRFRGDEAKADKVYEGTAPMTGEDIAEQVFFAATLPRHVVVLRIQA
EECCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCHHEEEEEEEH
MANQQSFSPFSIKR
HHCCCCCCCCCCCC
>Mature Secondary Structure 
SALDPKTLTVVVTGATSGIGAATVRRFASAGSKVVAMGRREDRLQALAQEFPNLVLPVV
CCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCEEEHHH
LDVRDYAATEAAFAALPDAFKGYNVLCANAGLALGLEPAQEAKIEDWQTMIDTNITGLAY
EEHHHHHHHHHHHHHCCHHHCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCEEE
TVRAGLPGMVARKEGHVVFTGSVAGDYPYPGGNVYGGTKAFVKQFALNVWADVVGTGVRV
EEECCCCCCEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
TNIEPGLTETEFSVVRFRGDEAKADKVYEGTAPMTGEDIAEQVFFAATLPRHVVVLRIQA
EECCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCHHEEEEEEEH
MANQQSFSPFSIKR
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]