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The map label for this gene is sir2 [H]

Identifier: 158422549

GI number: 158422549

Start: 1036406

End: 1038058

Strand: Reverse

Name: sir2 [H]

Synonym: AZC_0925

Alternate gene names: 158422549

Gene position: 1038058-1036406 (Counterclockwise)

Preceding gene: 158422550

Following gene: 158422548

Centisome position: 19.33

GC content: 65.94

Gene sequence:

>1653_bases
ATGTATGTGTATGACGAATTCGACCGGACCTTCCTGCAGGAGCGCGTGGATGAGTTCCGGGACCAGGTGCGCCGCCGCCT
CTCGGGCGAACTGAACGAGGAGGAGTTCAAGCCCCTGCGGCTCATGAACGGCGTCTACCTCCAGCTGCACGCCTATATGC
TGCGCATCGCCATTCCCTATGGCACGCTCTCGGCCGACCAGATGCGCGCGCTCGCCCATGTGGCCCGGCGCTATGACCGC
AACTACGGCCATTTCACCACCCGCCAGAACATCCAGTTCAACTGGATCAAGCTGGAAGAGCTGCCCGACGCCATGGCGGA
CCTCGCCCGCGCCGGCCTGCACGGGCTCCAGACCTCGGGCAACTGCATCCGCAACACCACCACCGATCCCTGGGCGGCGG
CGACGCCGGAGGAGATCGAGGATCCCCGCCTCTATGCGGAGATCATCCGGCAGTGGTCTACCCTCCACCCGGAATTCACC
TACCTGCCGCGCAAGTTCAAGATCGCCATCACGGCCGCCAAGCACGACCGTGCCGCCATCCGCGTGCACGACATCGGTCT
GCGCCTGCACCAGCGCGACGGCGAGACCGGCTTCGAGGTGATCGTCGGCGGCGGCCTCGGCCGCACGCCCTTCGTTGGCA
AGACCATCCGGGAATTCCTGCCGCGCCATCACCTGCTCTCCTATCTGGAGGCGGTGCTGCGCACCTATAATGCGGGCGGC
CGGCGCGACAACATCTACAAGGCGCGCATCAAGATCCAGGTGCACGAGATCGGCACCGAGGAATTCACCCGCCGCGTGGA
AGAAGAGTGGGCGCAGATCAAGGACGGCGCCCTCACCCTCGATGACGCCGCCATCGACGCCATCCGCGCCCATTTCAAGG
AGCCGGATTTCGAGGCGCTGACGGACGAGCCCGAGGTGCTGGTCGCTGCGAAGGCAGATAGCGCGTTCGCCCGCTGGTTC
GGCAATTCGGTGGCGCCGCACAAGGTGCCGGGCCATGCCATCGTCACCATCTCGCTGAAGCCGGTGGGCGGCCCTCCGGG
CGACGCAACGGCCGAGCAGATGGACGCCATTGCGGACCTCGCGGACCGCTTCGCCTATGGCGAGATCCGCGTGGCCCATG
AGCAGAACCTCACCCTGCCCCACGTGCGGCAGAAGGATCTGCCGGAGCTGTGGCGGGCGCTCGACAAGCTCGGCCTTGCC
ACCCCCAACGTGGGCCTGATCTCGGACATCATCGCCTGCCCGGGCCTCGACTATTGCTCGCTGGCCAATGCCCGCTCCAT
TCCGCTGGCGCAGGACCTGACCAAGCGCTTCGCGGACCACGAGCGGGCAGCGGACATCGGCCGCGTCCACCTCAACATCT
CGGGCTGCATCAATGCCTGCGGCCACCATCATGTGGGGCACATCGGCATTCTCGGCGTCGAGAAGAAGGACGAGGAATTC
TACCAGATCACGCTGGGCGGCAAGGCCAACCACAAGGCGCGCCTCGGCGAGCTGATCGGCCCGGCCGTTCCGGAATCGGA
AGTGGTGGACGTGGTGGAACGCATCCTCGATGCCTATGTCGAGCTGCGCCAGAGCCCGAGCGAGACCTTCATCGCCGCCG
TGCAGCGGCTTGGCATCGAACCTTTCAAGGAGCGCGCCTATGCCGCTCGTTAA

Upstream 100 bases:

>100_bases
GGCCGGCCGCATCCGGTCAACCTGCGCGAGCGCATCCGCGTTGGCGGCCCCACCATTGCCCTGCCGGGCGCCGCCTGAGC
GAGCGACGGAACCTGACCCC

Downstream 100 bases:

>100_bases
GCACGGAAAGCTCGTCACCGACGCTTTCGTGACCGTCGCTGACGACGAAACCCTGCCGGACGCGCCCGCCCTCGTCTCCG
CCGAGCGGCTGCTGAAGGAG

Product: nitrite/sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MYVYDEFDRTFLQERVDEFRDQVRRRLSGELNEEEFKPLRLMNGVYLQLHAYMLRIAIPYGTLSADQMRALAHVARRYDR
NYGHFTTRQNIQFNWIKLEELPDAMADLARAGLHGLQTSGNCIRNTTTDPWAAATPEEIEDPRLYAEIIRQWSTLHPEFT
YLPRKFKIAITAAKHDRAAIRVHDIGLRLHQRDGETGFEVIVGGGLGRTPFVGKTIREFLPRHHLLSYLEAVLRTYNAGG
RRDNIYKARIKIQVHEIGTEEFTRRVEEEWAQIKDGALTLDDAAIDAIRAHFKEPDFEALTDEPEVLVAAKADSAFARWF
GNSVAPHKVPGHAIVTISLKPVGGPPGDATAEQMDAIADLADRFAYGEIRVAHEQNLTLPHVRQKDLPELWRALDKLGLA
TPNVGLISDIIACPGLDYCSLANARSIPLAQDLTKRFADHERAADIGRVHLNISGCINACGHHHVGHIGILGVEKKDEEF
YQITLGGKANHKARLGELIGPAVPESEVVDVVERILDAYVELRQSPSETFIAAVQRLGIEPFKERAYAAR

Sequences:

>Translated_550_residues
MYVYDEFDRTFLQERVDEFRDQVRRRLSGELNEEEFKPLRLMNGVYLQLHAYMLRIAIPYGTLSADQMRALAHVARRYDR
NYGHFTTRQNIQFNWIKLEELPDAMADLARAGLHGLQTSGNCIRNTTTDPWAAATPEEIEDPRLYAEIIRQWSTLHPEFT
YLPRKFKIAITAAKHDRAAIRVHDIGLRLHQRDGETGFEVIVGGGLGRTPFVGKTIREFLPRHHLLSYLEAVLRTYNAGG
RRDNIYKARIKIQVHEIGTEEFTRRVEEEWAQIKDGALTLDDAAIDAIRAHFKEPDFEALTDEPEVLVAAKADSAFARWF
GNSVAPHKVPGHAIVTISLKPVGGPPGDATAEQMDAIADLADRFAYGEIRVAHEQNLTLPHVRQKDLPELWRALDKLGLA
TPNVGLISDIIACPGLDYCSLANARSIPLAQDLTKRFADHERAADIGRVHLNISGCINACGHHHVGHIGILGVEKKDEEF
YQITLGGKANHKARLGELIGPAVPESEVVDVVERILDAYVELRQSPSETFIAAVQRLGIEPFKERAYAAR
>Mature_550_residues
MYVYDEFDRTFLQERVDEFRDQVRRRLSGELNEEEFKPLRLMNGVYLQLHAYMLRIAIPYGTLSADQMRALAHVARRYDR
NYGHFTTRQNIQFNWIKLEELPDAMADLARAGLHGLQTSGNCIRNTTTDPWAAATPEEIEDPRLYAEIIRQWSTLHPEFT
YLPRKFKIAITAAKHDRAAIRVHDIGLRLHQRDGETGFEVIVGGGLGRTPFVGKTIREFLPRHHLLSYLEAVLRTYNAGG
RRDNIYKARIKIQVHEIGTEEFTRRVEEEWAQIKDGALTLDDAAIDAIRAHFKEPDFEALTDEPEVLVAAKADSAFARWF
GNSVAPHKVPGHAIVTISLKPVGGPPGDATAEQMDAIADLADRFAYGEIRVAHEQNLTLPHVRQKDLPELWRALDKLGLA
TPNVGLISDIIACPGLDYCSLANARSIPLAQDLTKRFADHERAADIGRVHLNISGCINACGHHHVGHIGILGVEKKDEEF
YQITLGGKANHKARLGELIGPAVPESEVVDVVERILDAYVELRQSPSETFIAAVQRLGIEPFKERAYAAR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=288, Percent_Identity=28.4722222222222, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 61895; Mature: 61895

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVYDEFDRTFLQERVDEFRDQVRRRLSGELNEEEFKPLRLMNGVYLQLHAYMLRIAIPY
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCC
GTLSADQMRALAHVARRYDRNYGHFTTRQNIQFNWIKLEELPDAMADLARAGLHGLQTSG
CCCCHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCC
NCIRNTTTDPWAAATPEEIEDPRLYAEIIRQWSTLHPEFTYLPRKFKIAITAAKHDRAAI
CEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCHHCCCCEEEEEEEEECCCCEEE
RVHDIGLRLHQRDGETGFEVIVGGGLGRTPFVGKTIREFLPRHHLLSYLEAVLRTYNAGG
EEEECCEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
RRDNIYKARIKIQVHEIGTEEFTRRVEEEWAQIKDGALTLDDAAIDAIRAHFKEPDFEAL
CCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCHHH
TDEPEVLVAAKADSAFARWFGNSVAPHKVPGHAIVTISLKPVGGPPGDATAEQMDAIADL
CCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHH
ADRFAYGEIRVAHEQNLTLPHVRQKDLPELWRALDKLGLATPNVGLISDIIACPGLDYCS
HHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHH
LANARSIPLAQDLTKRFADHERAADIGRVHLNISGCINACGHHHVGHIGILGVEKKDEEF
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCEEEEEECCCCCCCE
YQITLGGKANHKARLGELIGPAVPESEVVDVVERILDAYVELRQSPSETFIAAVQRLGIE
EEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
PFKERAYAAR
HHHHHHCCCC
>Mature Secondary Structure
MYVYDEFDRTFLQERVDEFRDQVRRRLSGELNEEEFKPLRLMNGVYLQLHAYMLRIAIPY
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCC
GTLSADQMRALAHVARRYDRNYGHFTTRQNIQFNWIKLEELPDAMADLARAGLHGLQTSG
CCCCHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCC
NCIRNTTTDPWAAATPEEIEDPRLYAEIIRQWSTLHPEFTYLPRKFKIAITAAKHDRAAI
CEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCHHCCCCEEEEEEEEECCCCEEE
RVHDIGLRLHQRDGETGFEVIVGGGLGRTPFVGKTIREFLPRHHLLSYLEAVLRTYNAGG
EEEECCEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
RRDNIYKARIKIQVHEIGTEEFTRRVEEEWAQIKDGALTLDDAAIDAIRAHFKEPDFEAL
CCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCHHH
TDEPEVLVAAKADSAFARWFGNSVAPHKVPGHAIVTISLKPVGGPPGDATAEQMDAIADL
CCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHH
ADRFAYGEIRVAHEQNLTLPHVRQKDLPELWRALDKLGLATPNVGLISDIIACPGLDYCS
HHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHH
LANARSIPLAQDLTKRFADHERAADIGRVHLNISGCINACGHHHVGHIGILGVEKKDEEF
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCEEEEEECCCCCCCE
YQITLGGKANHKARLGELIGPAVPESEVVDVVERILDAYVELRQSPSETFIAAVQRLGIE
EEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
PFKERAYAAR
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA