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The map label for this gene is ispB [H]

Identifier: 158422531

GI number: 158422531

Start: 1014498

End: 1015505

Strand: Direct

Name: ispB [H]

Synonym: AZC_0907

Alternate gene names: 158422531

Gene position: 1014498-1015505 (Clockwise)

Preceding gene: 158422524

Following gene: 158422532

Centisome position: 18.89

GC content: 66.87

Gene sequence:

>1008_bases
TTGGCCGTCGTTCTTCCGTTTGAGCCCAAGGAGCCCTCCATCGAGACGCTGGTGCGGCTGGTGAAGGGCGACATGGAGCG
CGTGAATGCGCTGATCCTGTCGCGCACCGGCTCCGACGTGGCGATGATTCCCGAGGTTGCGAACCACCTGATCTCCTCGG
GTGGCAAGCGCCTGCGCCCCATGCTGACCATCGCCTGCGCGGCGCTGTCGAGCTATGAGGGCGACGGCCATATCAAGCTC
GCCGCCTCGGTGGAGTTCATGCACACGGCGACCCTCCTCCACGACGACGTGGTGGACGAGAGCGACATGCGCCGGGGCAA
GCTCGCCGCCCGCAAGCTGTGGGGCAATGAGGCGAGCGTGCTGGTGGGCGACTTCCTGCTCGGCCAGGCCTTCAAGATGA
TGGTGGAAGTGGGCATTCTCGAATGCCTCGACATCCTCTCCACCGCCGCCTGCACCATCGCCGAAGGCGAGGTGATGCAG
CTTGCCGCCGCCAAGAATACCGAGACCTCGGAAGACGATTATCTCGCCGTGATCCGCGGCAAGACGGCCGAGCTCTTCGC
CGCCGCCTGCGAGGTTGGCGCGGTGCTGAATGCCCGGCCCAAGGCGGAGCAGGCGGCCTGCCGCTCCTTCGGCATGAATC
TCGGCATCGCCTTCCAGCTGGTGGACGACGCGCTGGACTATGGCGGCAGCCAGGCCAAGCTCGGCAAGAATGTCGGCGAC
GATTTTCGCGAGGGCAAGATCACCCTTCCCGTGCTCCTCGCCTTCCGGCGTGGCGATGCGGAGGAGCGCGCCTTCTGGAA
GCGCTGCCTCGAGACGGGCGAGACCACGGACGAGAATCTCGCCCATGCGGTCGGCCTGCTGACCAAGCATCGCGCGCTGG
CCGACACCATCGAGCGGGCGCGGCATTATGGCGCCATGGCCAAGGATGCCCTCGCCCTGTTCCCCGCGAGCCACGCCAAG
GACGCGCTGGCCGAGGCGGTGGACTTCTGCATCTCCCGCGCCCACTGA

Upstream 100 bases:

>100_bases
TTTATCGTGCTTTCACGGCCCCAGCGGCACGTGGGCGCTTGCACGGACCCTAGGAGCATCCCTATGGTGCGCCCCGCTGA
ATGACAAGCGGGAGCAATCA

Downstream 100 bases:

>100_bases
GGTCGCCCGCGTCCGGCAGCGACCGGGCGTAAATCGAATGATGGGCCGCGCCGGCCGCCTTCCGGCCGGCCCTTCTTTTG
CCGGCGAGAACAACCTGAGC

Product: farnesyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAVVLPFEPKEPSIETLVRLVKGDMERVNALILSRTGSDVAMIPEVANHLISSGGKRLRPMLTIACAALSSYEGDGHIKL
AASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASVLVGDFLLGQAFKMMVEVGILECLDILSTAACTIAEGEVMQ
LAAAKNTETSEDDYLAVIRGKTAELFAAACEVGAVLNARPKAEQAACRSFGMNLGIAFQLVDDALDYGGSQAKLGKNVGD
DFREGKITLPVLLAFRRGDAEERAFWKRCLETGETTDENLAHAVGLLTKHRALADTIERARHYGAMAKDALALFPASHAK
DALAEAVDFCISRAH

Sequences:

>Translated_335_residues
MAVVLPFEPKEPSIETLVRLVKGDMERVNALILSRTGSDVAMIPEVANHLISSGGKRLRPMLTIACAALSSYEGDGHIKL
AASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASVLVGDFLLGQAFKMMVEVGILECLDILSTAACTIAEGEVMQ
LAAAKNTETSEDDYLAVIRGKTAELFAAACEVGAVLNARPKAEQAACRSFGMNLGIAFQLVDDALDYGGSQAKLGKNVGD
DFREGKITLPVLLAFRRGDAEERAFWKRCLETGETTDENLAHAVGLLTKHRALADTIERARHYGAMAKDALALFPASHAK
DALAEAVDFCISRAH
>Mature_334_residues
AVVLPFEPKEPSIETLVRLVKGDMERVNALILSRTGSDVAMIPEVANHLISSGGKRLRPMLTIACAALSSYEGDGHIKLA
ASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASVLVGDFLLGQAFKMMVEVGILECLDILSTAACTIAEGEVMQL
AAAKNTETSEDDYLAVIRGKTAELFAAACEVGAVLNARPKAEQAACRSFGMNLGIAFQLVDDALDYGGSQAKLGKNVGDD
FREGKITLPVLLAFRRGDAEERAFWKRCLETGETTDENLAHAVGLLTKHRALADTIERARHYGAMAKDALALFPASHAKD
ALAEAVDFCISRAH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=243, Percent_Identity=31.6872427983539, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=42.0560747663551, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1786623, Length=224, Percent_Identity=32.1428571428571, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17505681, Length=240, Percent_Identity=33.75, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319475, Length=303, Percent_Identity=31.6831683168317, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=251, Percent_Identity=22.7091633466135, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24651612, Length=250, Percent_Identity=31.2, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36071; Mature: 35940

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVLPFEPKEPSIETLVRLVKGDMERVNALILSRTGSDVAMIPEVANHLISSGGKRLRP
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHHH
MLTIACAALSSYEGDGHIKLAASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASV
HHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
LVGDFLLGQAFKMMVEVGILECLDILSTAACTIAEGEVMQLAAAKNTETSEDDYLAVIRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEC
KTAELFAAACEVGAVLNARPKAEQAACRSFGMNLGIAFQLVDDALDYGGSQAKLGKNVGD
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
DFREGKITLPVLLAFRRGDAEERAFWKRCLETGETTDENLAHAVGLLTKHRALADTIERA
HHHCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHYGAMAKDALALFPASHAKDALAEAVDFCISRAH
HHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVVLPFEPKEPSIETLVRLVKGDMERVNALILSRTGSDVAMIPEVANHLISSGGKRLRP
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHHH
MLTIACAALSSYEGDGHIKLAASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASV
HHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
LVGDFLLGQAFKMMVEVGILECLDILSTAACTIAEGEVMQLAAAKNTETSEDDYLAVIRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEC
KTAELFAAACEVGAVLNARPKAEQAACRSFGMNLGIAFQLVDDALDYGGSQAKLGKNVGD
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
DFREGKITLPVLLAFRRGDAEERAFWKRCLETGETTDENLAHAVGLLTKHRALADTIERA
HHHCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHYGAMAKDALALFPASHAKDALAEAVDFCISRAH
HHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]