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The map label for this gene is lysR [H]

Identifier: 158422437

GI number: 158422437

Start: 917853

End: 918782

Strand: Direct

Name: lysR [H]

Synonym: AZC_0813

Alternate gene names: 158422437

Gene position: 917853-918782 (Clockwise)

Preceding gene: 158422428

Following gene: 158422439

Centisome position: 17.09

GC content: 68.82

Gene sequence:

>930_bases
TTGGGCGACGCCGCACTGCGGTACTTCCTTGAAGTTGTTCGCTCCGGCTCCATTGCCGAGGCGTCCGTGCGACTGAACGT
GGCCGCCTCCGCCATCAGCAGGCAGATCGCAAAACTGGAAGCCGAACTGGGGTCGCCTCTGTTCGAGCGGCGCTCGCGCG
GCATGGTGCCGAGCGCGGCGGGCGAACTTCTGGCCCAGCATGCCCGGCGCACGCTGCTGGGCGAGGAGCAGGTGGTGAGC
GAAATCCGCCGCCTGCGTGGCCTCGAGCGCGGCCTCGTGCGGGTGGGCTGCACGGAAGGGTTTGCCACCGATTTCATGCC
GGAGGCCATCGGCATGTTCCGGCATACCTATCCCGGCATCGCCTTCGACATGCGGGTGGCCGCGCCGCAGGCGGTGACAC
GCATGGTCCGCGAGGGCGAGATCGATATCGGCCTCACCTTCGGCTTCATGCCGGACGCGAGCGTCCAGGTGGAGTTCGGC
GGCAGCGCGCCGCTGATGGCGCTGGTGGGGCTCGACCATCCGCTGGCCAACCGGCCGTGGGTGACGATCGCGGACATCGC
CGCCTTTCCCCTCGGCCTGCCGGCCAAGGACACGACCGCACGCCAGCTGTTCGATCTGGTCTGCGGTGCGGAGGGGATCG
CCGTTGAGCCCATGCTCACGACCAACTACATGTCCGGCCTGTGGCGGTTCGCGGAGACGGGCGGCGGCGTGACGGTGACG
GGGCGCATCACCGTGCTCTCGCGCATCTGGCGCTTCCACGTGGTGGCCTTGCCGCTCAGGAGCAAGACGTCAAACGAGCG
GCGCTACGAGATCCAGTCCATGCTGGGACGCACCTTGCCGGACGCGGCGCGGGCCTTCGTCTTTCACCTGCGGCGCCATC
TGGAACAACTGGACCGCCCCGGCGACCAGCCTCTGGGCCTGCGGTCCTAG

Upstream 100 bases:

>100_bases
GCAGCTTATTGAGCCTTTCGGATATCGCAATTAGAATATTTCGTGATTATCGTTGCCGTTTCGGCAATGAAGCCCTGTCG
CGCTGCACCCGGGGGTTACG

Downstream 100 bases:

>100_bases
GGCCGTTTCGTCCGAAAAATCAGTTCTGCGCGGCGGCTTCTGTGCCAGCCCCATGGCTCATGGCATCGAAAAGCGCGGCG
ACGCTTTCGATCCGCGACAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MGDAALRYFLEVVRSGSIAEASVRLNVAASAISRQIAKLEAELGSPLFERRSRGMVPSAAGELLAQHARRTLLGEEQVVS
EIRRLRGLERGLVRVGCTEGFATDFMPEAIGMFRHTYPGIAFDMRVAAPQAVTRMVREGEIDIGLTFGFMPDASVQVEFG
GSAPLMALVGLDHPLANRPWVTIADIAAFPLGLPAKDTTARQLFDLVCGAEGIAVEPMLTTNYMSGLWRFAETGGGVTVT
GRITVLSRIWRFHVVALPLRSKTSNERRYEIQSMLGRTLPDAARAFVFHLRRHLEQLDRPGDQPLGLRS

Sequences:

>Translated_309_residues
MGDAALRYFLEVVRSGSIAEASVRLNVAASAISRQIAKLEAELGSPLFERRSRGMVPSAAGELLAQHARRTLLGEEQVVS
EIRRLRGLERGLVRVGCTEGFATDFMPEAIGMFRHTYPGIAFDMRVAAPQAVTRMVREGEIDIGLTFGFMPDASVQVEFG
GSAPLMALVGLDHPLANRPWVTIADIAAFPLGLPAKDTTARQLFDLVCGAEGIAVEPMLTTNYMSGLWRFAETGGGVTVT
GRITVLSRIWRFHVVALPLRSKTSNERRYEIQSMLGRTLPDAARAFVFHLRRHLEQLDRPGDQPLGLRS
>Mature_308_residues
GDAALRYFLEVVRSGSIAEASVRLNVAASAISRQIAKLEAELGSPLFERRSRGMVPSAAGELLAQHARRTLLGEEQVVSE
IRRLRGLERGLVRVGCTEGFATDFMPEAIGMFRHTYPGIAFDMRVAAPQAVTRMVREGEIDIGLTFGFMPDASVQVEFGG
SAPLMALVGLDHPLANRPWVTIADIAAFPLGLPAKDTTARQLFDLVCGAEGIAVEPMLTTNYMSGLWRFAETGGGVTVTG
RITVLSRIWRFHVVALPLRSKTSNERRYEIQSMLGRTLPDAARAFVFHLRRHLEQLDRPGDQPLGLRS

Specific function: Positive regulator of glutamate biosynthesis (gltAB genes). Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=222, Percent_Identity=27.4774774774775, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787879, Length=187, Percent_Identity=30.4812834224599, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI145693105, Length=188, Percent_Identity=26.063829787234, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788481, Length=200, Percent_Identity=31.5, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788748, Length=234, Percent_Identity=25.2136752136752, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789204, Length=249, Percent_Identity=23.2931726907631, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=33.5664335664336, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081904, Length=214, Percent_Identity=27.1028037383178, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788887, Length=183, Percent_Identity=27.8688524590164, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=26.2295081967213, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788297, Length=152, Percent_Identity=29.6052631578947, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=151, Percent_Identity=29.1390728476821, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789639, Length=252, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787530, Length=245, Percent_Identity=23.6734693877551, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789440, Length=115, Percent_Identity=26.9565217391304, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=180, Percent_Identity=24.4444444444444, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367136, Length=140, Percent_Identity=29.2857142857143, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33857; Mature: 33725

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDAALRYFLEVVRSGSIAEASVRLNVAASAISRQIAKLEAELGSPLFERRSRGMVPSAA
CCHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHH
GELLAQHARRTLLGEEQVVSEIRRLRGLERGLVRVGCTEGFATDFMPEAIGMFRHTYPGI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCC
AFDMRVAAPQAVTRMVREGEIDIGLTFGFMPDASVQVEFGGSAPLMALVGLDHPLANRPW
EEEEHHCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCHHHEECCCCCCCCCCC
VTIADIAAFPLGLPAKDTTARQLFDLVCGAEGIAVEPMLTTNYMSGLWRFAETGGGVTVT
EEEHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEE
GRITVLSRIWRFHVVALPLRSKTSNERRYEIQSMLGRTLPDAARAFVFHLRRHLEQLDRP
HHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
GDQPLGLRS
CCCCCCCCC
>Mature Secondary Structure 
GDAALRYFLEVVRSGSIAEASVRLNVAASAISRQIAKLEAELGSPLFERRSRGMVPSAA
CHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHH
GELLAQHARRTLLGEEQVVSEIRRLRGLERGLVRVGCTEGFATDFMPEAIGMFRHTYPGI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCC
AFDMRVAAPQAVTRMVREGEIDIGLTFGFMPDASVQVEFGGSAPLMALVGLDHPLANRPW
EEEEHHCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCHHHEECCCCCCCCCCC
VTIADIAAFPLGLPAKDTTARQLFDLVCGAEGIAVEPMLTTNYMSGLWRFAETGGGVTVT
EEEHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEE
GRITVLSRIWRFHVVALPLRSKTSNERRYEIQSMLGRTLPDAARAFVFHLRRHLEQLDRP
HHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
GDQPLGLRS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2548995; 9384377 [H]