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The map label for this gene is gsiB [H]

Identifier: 158422436

GI number: 158422436

Start: 916165

End: 917751

Strand: Reverse

Name: gsiB [H]

Synonym: AZC_0812

Alternate gene names: 158422436

Gene position: 917751-916165 (Counterclockwise)

Preceding gene: 158422438

Following gene: 158422435

Centisome position: 17.09

GC content: 63.52

Gene sequence:

>1587_bases
ATGCCTAGATGTTGGGCGAACAGCACCGGCATCCAATATCAAACCCAGAGAGAGAACATGGCTTTCCTTCAGTCCCGTCT
CTCACGTGCTCTCGCGGTGGCCATGTTCGCCATCGCTCCGGCGGCCGCTCACGCGGGCAAGGCCAACGACACGCTCGTCT
ATGCCTCGGATTCCGAGCCGGAGAACGTCAGCCCCTATCATAACAACGTGCGGGAAGGCGTCATCATCGCCCGCCACGTG
TTCGACAACCTGATCTTCCACGATCTGGCCACCGGCAAATACGAGCCGCAGCTCGCCACCAACTGGACCTGGGCGGATCC
GCAGACGCTGGACCTCACCATCCGCAAGGGCGTCACCTTCCACAATGGCGACCCGCTGACCGCCGACGACGTGGTATTCA
CGCTCAACTACGTGGTGTCGCCGGACTCCAAGGTGGTGACCAAGCAGAACGTCAACTGGATCAAGAGCGCCGAGAAGATT
GGCGACGACAAGGTCCGCATCCACCTCGTGGGCCCGTTCCCGGCGGCCATCGAATATCTCGCCGGCCCGGTGGTCATCTA
TCCGGAGAAGTACTTCAAGAAGGTCGGCCTCGACGGCTTCGCCAAGGCCCCCATCGGCTCCGGACCCTACAAGGTGACTT
CGGTCACGCCCGGCAAGGGCGTGAAGATGGAAAAGAACACCAGCTACTGGAAGGACAGCCCGCTCGGCCAGCCGAAGATC
GGCAAGCTGGAATTCCGCATCATCCCCGATCCGGAGACCCAGGTGGCCGAACTGATGACCGGCGGCGTGGACTGGATCTG
GCGCGTGCAGGCCGACCAGGCCAAGCAGCTCGGCGCCGTGCCCAACCTCACCGTGCTCTCGGCCGAGACCATGCGCGTCG
GCTTCCTCCAGTTCGACTCGCAGGGCCGTGCTCCCGGCTCCGAGCCGCTGAAGGATCCCCGCGTCCGCCAGGCCATCTCC
TATGCCATCGACCGCAAGGCCATGGTGGACAATCTGGTGCGCGGCGGCGCCCGCGTCATGAACTCGGCCTGCTTCATCGA
TCAGTTCGGCTGCACCGACAAGGGCGTGCCGCGCTATGACTATGATCCCGCCAAGGCCAAGAAGCTGCTGGCCGACGCCG
GCTATCCCAACGGCTTCTCCATCGACCTCTACGCCTATCGCGAGCGCGAATATGCGGAAGCGGTGGTCGGCTATCTTGGC
GCCGTCGGCATCAAGGCGAAGCTGAACTTCATGAAGTATGCGGCCCTGCGCGAGCTGTCCCGCTCCGGCAAGGTTCCGAT
CTACTTCCAGACCTGGGGCTCCTTCTCGGTGGCCGATGCCTCCGCCTTCACCGGCGTGTGGTTCAAGGGCGACGAGGACG
ACATGACCCGCGACCAGCAGGTCACGGACATGCTCAAGAAGGCGGATACGACGGTGGAGACGGAAGCGCGGCTTGCCGCC
TATTCCGAGGCGTTGAAGCTCATCGCCGAGCGCGCCTACCTGCTGCCGCTCTTCTCCTATTCGTCCAACTACGCCTTCAC
CAGCGACCTGAACTTCAACGCCCAGCCCGACGAGCTGCCGCGCTTCTTCAAGGCCCGCTGGCAGTGA

Upstream 100 bases:

>100_bases
GTAACCCCCGGGTGCAGCGCGACAGGGCTTCATTGCCGAAACGGCAACGATAATCACGAAATATTCTAATTGCGATATCC
GAAAGGCTCAATAAGCTGCC

Downstream 100 bases:

>100_bases
CCTCATGGACGGCGCGCCTTGGGCGCGCCGTCCGCCGACCGTGGCGTGCCTAAAGGCCCGCCCGGCCCGTCCGCCGCGAT
GGCGCTCATGCGCCCGGCGT

Product: peptide ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MPRCWANSTGIQYQTQRENMAFLQSRLSRALAVAMFAIAPAAAHAGKANDTLVYASDSEPENVSPYHNNVREGVIIARHV
FDNLIFHDLATGKYEPQLATNWTWADPQTLDLTIRKGVTFHNGDPLTADDVVFTLNYVVSPDSKVVTKQNVNWIKSAEKI
GDDKVRIHLVGPFPAAIEYLAGPVVIYPEKYFKKVGLDGFAKAPIGSGPYKVTSVTPGKGVKMEKNTSYWKDSPLGQPKI
GKLEFRIIPDPETQVAELMTGGVDWIWRVQADQAKQLGAVPNLTVLSAETMRVGFLQFDSQGRAPGSEPLKDPRVRQAIS
YAIDRKAMVDNLVRGGARVMNSACFIDQFGCTDKGVPRYDYDPAKAKKLLADAGYPNGFSIDLYAYREREYAEAVVGYLG
AVGIKAKLNFMKYAALRELSRSGKVPIYFQTWGSFSVADASAFTGVWFKGDEDDMTRDQQVTDMLKKADTTVETEARLAA
YSEALKLIAERAYLLPLFSYSSNYAFTSDLNFNAQPDELPRFFKARWQ

Sequences:

>Translated_528_residues
MPRCWANSTGIQYQTQRENMAFLQSRLSRALAVAMFAIAPAAAHAGKANDTLVYASDSEPENVSPYHNNVREGVIIARHV
FDNLIFHDLATGKYEPQLATNWTWADPQTLDLTIRKGVTFHNGDPLTADDVVFTLNYVVSPDSKVVTKQNVNWIKSAEKI
GDDKVRIHLVGPFPAAIEYLAGPVVIYPEKYFKKVGLDGFAKAPIGSGPYKVTSVTPGKGVKMEKNTSYWKDSPLGQPKI
GKLEFRIIPDPETQVAELMTGGVDWIWRVQADQAKQLGAVPNLTVLSAETMRVGFLQFDSQGRAPGSEPLKDPRVRQAIS
YAIDRKAMVDNLVRGGARVMNSACFIDQFGCTDKGVPRYDYDPAKAKKLLADAGYPNGFSIDLYAYREREYAEAVVGYLG
AVGIKAKLNFMKYAALRELSRSGKVPIYFQTWGSFSVADASAFTGVWFKGDEDDMTRDQQVTDMLKKADTTVETEARLAA
YSEALKLIAERAYLLPLFSYSSNYAFTSDLNFNAQPDELPRFFKARWQ
>Mature_527_residues
PRCWANSTGIQYQTQRENMAFLQSRLSRALAVAMFAIAPAAAHAGKANDTLVYASDSEPENVSPYHNNVREGVIIARHVF
DNLIFHDLATGKYEPQLATNWTWADPQTLDLTIRKGVTFHNGDPLTADDVVFTLNYVVSPDSKVVTKQNVNWIKSAEKIG
DDKVRIHLVGPFPAAIEYLAGPVVIYPEKYFKKVGLDGFAKAPIGSGPYKVTSVTPGKGVKMEKNTSYWKDSPLGQPKIG
KLEFRIIPDPETQVAELMTGGVDWIWRVQADQAKQLGAVPNLTVLSAETMRVGFLQFDSQGRAPGSEPLKDPRVRQAISY
AIDRKAMVDNLVRGGARVMNSACFIDQFGCTDKGVPRYDYDPAKAKKLLADAGYPNGFSIDLYAYREREYAEAVVGYLGA
VGIKAKLNFMKYAALRELSRSGKVPIYFQTWGSFSVADASAFTGVWFKGDEDDMTRDQQVTDMLKKADTTVETEARLAAY
SEALKLIAERAYLLPLFSYSSNYAFTSDLNFNAQPDELPRFFKARWQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=495, Percent_Identity=27.4747474747475, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789966, Length=510, Percent_Identity=24.5098039215686, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789887, Length=523, Percent_Identity=25.0478011472275, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787762, Length=490, Percent_Identity=23.0612244897959, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787551, Length=510, Percent_Identity=22.3529411764706, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082063, Length=158, Percent_Identity=31.0126582278481, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58681; Mature: 58550

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRCWANSTGIQYQTQRENMAFLQSRLSRALAVAMFAIAPAAAHAGKANDTLVYASDSEP
CCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
ENVSPYHNNVREGVIIARHVFDNLIFHDLATGKYEPQLATNWTWADPQTLDLTIRKGVTF
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCEEE
HNGDPLTADDVVFTLNYVVSPDSKVVTKQNVNWIKSAEKIGDDKVRIHLVGPFPAAIEYL
ECCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHH
AGPVVIYPEKYFKKVGLDGFAKAPIGSGPYKVTSVTPGKGVKMEKNTSYWKDSPLGQPKI
CCCEEECCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCC
GKLEFRIIPDPETQVAELMTGGVDWIWRVQADQAKQLGAVPNLTVLSAETMRVGFLQFDS
CCEEEEEECCCHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCEEEEECCCEEEEEEEECC
QGRAPGSEPLKDPRVRQAISYAIDRKAMVDNLVRGGARVMNSACFIDQFGCTDKGVPRYD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCCCCCCC
YDPAKAKKLLADAGYPNGFSIDLYAYREREYAEAVVGYLGAVGIKAKLNFMKYAALRELS
CCHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RSGKVPIYFQTWGSFSVADASAFTGVWFKGDEDDMTRDQQVTDMLKKADTTVETEARLAA
HCCCCEEEEEECCCEEECCCCHHEEEEEECCCHHCCHHHHHHHHHHHCCCCHHHHHHHHH
YSEALKLIAERAYLLPLFSYSSNYAFTSDLNFNAQPDELPRFFKARWQ
HHHHHHHHHHHHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PRCWANSTGIQYQTQRENMAFLQSRLSRALAVAMFAIAPAAAHAGKANDTLVYASDSEP
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
ENVSPYHNNVREGVIIARHVFDNLIFHDLATGKYEPQLATNWTWADPQTLDLTIRKGVTF
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCEEE
HNGDPLTADDVVFTLNYVVSPDSKVVTKQNVNWIKSAEKIGDDKVRIHLVGPFPAAIEYL
ECCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHH
AGPVVIYPEKYFKKVGLDGFAKAPIGSGPYKVTSVTPGKGVKMEKNTSYWKDSPLGQPKI
CCCEEECCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCC
GKLEFRIIPDPETQVAELMTGGVDWIWRVQADQAKQLGAVPNLTVLSAETMRVGFLQFDS
CCEEEEEECCCHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCEEEEECCCEEEEEEEECC
QGRAPGSEPLKDPRVRQAISYAIDRKAMVDNLVRGGARVMNSACFIDQFGCTDKGVPRYD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCCCCCCC
YDPAKAKKLLADAGYPNGFSIDLYAYREREYAEAVVGYLGAVGIKAKLNFMKYAALRELS
CCHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RSGKVPIYFQTWGSFSVADASAFTGVWFKGDEDDMTRDQQVTDMLKKADTTVETEARLAA
HCCCCEEEEEECCCEEECCCCHHEEEEEECCCHHCCHHHHHHHHHHHCCCCHHHHHHHHH
YSEALKLIAERAYLLPLFSYSSNYAFTSDLNFNAQPDELPRFFKARWQ
HHHHHHHHHHHHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA