BacMap

Definition	Azorhizobium caulinodans ORS 571, complete genome.
Accession	NC_009937
Length	5,369,772

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The map label for this gene is fadB1 [H]

Identifier: 158422426

GI number: 158422426

Start: 905244

End: 906014

Strand: Direct

Name: fadB1 [H]

Synonym: AZC_0802

Alternate gene names: 158422426

Gene position: 905244-906014 (Clockwise)

Preceding gene: 158422425

Following gene: 158422428

Centisome position: 16.86

GC content: 65.63

Gene sequence:

>771_bases
ATGGCTTACGAGACGATCCTGGTCGAAACCCATGGCAAGGTTGGCCTCATCCGCCTGAACCGGCCGCAGGCGCTGAACGC
GCTCAATCGCCAGATCATCACCGAGCTGAATGCCGCCCTCGATGCCTTCGAGGCCGACACGGGCATCGGCTGCATCATCC
TCACCGGCTCGGAAAAGGCCTTCGCCGCCGGCGCCGACATCAAGGAGATGCAGGACTTCACCTATCCCCAGACCTATCTG
GACGACTTCATCGCCTCCTGGGAGCGCCTCTCGCGCACCCGCAAGCCGGTGATCGCGGCGGTGGCGGGCTTCGCGCTCGG
CGGCGGCTGCGAACTCGCCATGATGTGCGACTTCATCCTCGCCGCGGACACGGCAAAGTTCGGCCAGCCGGAGATCAAGC
TCGGCGTGATGCCTGGCGCCGGGGGCACCCAGCGCCTGACGCGCTTCGTCGGCAAGTCCAAGGCCATGGAGATGTGCCTC
ACCGGCCGCTTCATGGATGCGGCGGAGGCCGAACGGGCCGGGCTCGTCTCCCGCGTCATTCCCGCTGCCGATCTGGTGGA
CGAGGCGCTGAAGGTGGCGGCGGTCATTGCCGACATGTCGCGCCCCGTCGCCATGATGACCAAGGAGAGCGTGAACCGCT
CGTACGAGACCACGCTCACCGAAGGCATCCGCTTCGAGCGGCGGGTCTTCCATTCCATGTTCGCACTGGCGGATCAGAAG
GAAGGCATGAGCGCCTTCGTCGAGAAGCGGAAGCCGAATTTCGGCGGCTGA

Upstream 100 bases:

>100_bases
CGCGCTCTAGACAGGCGAGACGCCGCTCAGCGGTGACATCCGGTTCATAGGGCCGATGAACCGGACGGCGAACAGCGGCC
CTGGCTTCATGGAGACGCGC

Downstream 100 bases:

>100_bases
TGGCCCGATAATCCTTGCGGCGCCTCGCTTCCGGTGGGGCGCCGTGAGGGGGCTGGCCGGTGTTACCCACAGGCTCTCCA
TCTTTCCTTGGCCGGCTCGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAYETILVETHGKVGLIRLNRPQALNALNRQIITELNAALDAFEADTGIGCIILTGSEKAFAAGADIKEMQDFTYPQTYL
DDFIASWERLSRTRKPVIAAVAGFALGGGCELAMMCDFILAADTAKFGQPEIKLGVMPGAGGTQRLTRFVGKSKAMEMCL
TGRFMDAAEAERAGLVSRVIPAADLVDEALKVAAVIADMSRPVAMMTKESVNRSYETTLTEGIRFERRVFHSMFALADQK
EGMSAFVEKRKPNFGG

Sequences:

>Translated_256_residues
MAYETILVETHGKVGLIRLNRPQALNALNRQIITELNAALDAFEADTGIGCIILTGSEKAFAAGADIKEMQDFTYPQTYL
DDFIASWERLSRTRKPVIAAVAGFALGGGCELAMMCDFILAADTAKFGQPEIKLGVMPGAGGTQRLTRFVGKSKAMEMCL
TGRFMDAAEAERAGLVSRVIPAADLVDEALKVAAVIADMSRPVAMMTKESVNRSYETTLTEGIRFERRVFHSMFALADQK
EGMSAFVEKRKPNFGG
>Mature_255_residues
AYETILVETHGKVGLIRLNRPQALNALNRQIITELNAALDAFEADTGIGCIILTGSEKAFAAGADIKEMQDFTYPQTYLD
DFIASWERLSRTRKPVIAAVAGFALGGGCELAMMCDFILAADTAKFGQPEIKLGVMPGAGGTQRLTRFVGKSKAMEMCLT
GRFMDAAEAERAGLVSRVIPAADLVDEALKVAAVIADMSRPVAMMTKESVNRSYETTLTEGIRFERRVFHSMFALADQKE
GMSAFVEKRKPNFGG

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=58.0392156862745, Blast_Score=299, Evalue=2e-81,
Organism=Homo sapiens, GI68989263, Length=188, Percent_Identity=36.1702127659575, Blast_Score=121, Evalue=5e-28,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=35, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI31542718, Length=240, Percent_Identity=30.8333333333333, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI20127408, Length=191, Percent_Identity=35.0785340314136, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=30.4511278195489, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI150378541, Length=245, Percent_Identity=30.2040816326531, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI37594471, Length=196, Percent_Identity=30.6122448979592, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594469, Length=191, Percent_Identity=30.3664921465969, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=26.8181818181818, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=26.8181818181818, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=26.8181818181818, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI62530384, Length=222, Percent_Identity=27.9279279279279, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI261878539, Length=94, Percent_Identity=40.4255319148936, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI25453481, Length=262, Percent_Identity=24.4274809160305, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=262, Percent_Identity=24.4274809160305, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757968, Length=267, Percent_Identity=25.8426966292135, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=267, Percent_Identity=25.8426966292135, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157694520, Length=190, Percent_Identity=26.8421052631579, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136756, Length=262, Percent_Identity=24.4274809160305, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=262, Percent_Identity=24.4274809160305, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757966, Length=198, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=198, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=24.7899159663866, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI295842266, Length=220, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=45.2380952380952, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=36.5019011406844, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=35.6, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=34.3629343629344, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=33.2075471698113, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=35.195530726257, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=31.7307692307692, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=55.46875, Blast_Score=290, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=52.0661157024793, Blast_Score=255, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=30.8016877637131, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=244, Percent_Identity=34.0163934426229, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17558304, Length=193, Percent_Identity=33.160621761658, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=205, Percent_Identity=34.1463414634146, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17508953, Length=205, Percent_Identity=34.1463414634146, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17508951, Length=205, Percent_Identity=34.1463414634146, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17540306, Length=235, Percent_Identity=32.7659574468085, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144160, Length=201, Percent_Identity=32.3383084577114, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI25144157, Length=201, Percent_Identity=32.3383084577114, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=257, Percent_Identity=28.7937743190661, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=258, Percent_Identity=29.8449612403101, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=265, Percent_Identity=27.1698113207547, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=23.6220472440945, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=26.2886597938144, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=59.2156862745098, Blast_Score=307, Evalue=5e-84,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=59.2156862745098, Blast_Score=307, Evalue=5e-84,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=35.5731225296443, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=29.8039215686275, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=30.859375, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650670, Length=212, Percent_Identity=34.9056603773585, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19920382, Length=224, Percent_Identity=36.1607142857143, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571730, Length=238, Percent_Identity=26.0504201680672, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571729, Length=238, Percent_Identity=26.0504201680672, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583077, Length=175, Percent_Identity=32, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583079, Length=175, Percent_Identity=32, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921000, Length=175, Percent_Identity=32, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921018, Length=204, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583165, Length=173, Percent_Identity=27.1676300578035, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27803; Mature: 27671

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYETILVETHGKVGLIRLNRPQALNALNRQIITELNAALDAFEADTGIGCIILTGSEKA
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
FAAGADIKEMQDFTYPQTYLDDFIASWERLSRTRKPVIAAVAGFALGGGCELAMMCDFIL
EECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEIKLGVMPGAGGTQRLTRFVGKSKAMEMCLTGRFMDAAEAERAGLVSRVI
HHCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
PAADLVDEALKVAAVIADMSRPVAMMTKESVNRSYETTLTEGIRFERRVFHSMFALADQK
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGMSAFVEKRKPNFGG
CHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AYETILVETHGKVGLIRLNRPQALNALNRQIITELNAALDAFEADTGIGCIILTGSEKA
CCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
FAAGADIKEMQDFTYPQTYLDDFIASWERLSRTRKPVIAAVAGFALGGGCELAMMCDFIL
EECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEIKLGVMPGAGGTQRLTRFVGKSKAMEMCLTGRFMDAAEAERAGLVSRVI
HHCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
PAADLVDEALKVAAVIADMSRPVAMMTKESVNRSYETTLTEGIRFERRVFHSMFALADQK
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGMSAFVEKRKPNFGG
CHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]