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The map label for this gene is dapE [H]

Identifier: 158422417

GI number: 158422417

Start: 895692

End: 896921

Strand: Direct

Name: dapE [H]

Synonym: AZC_0793

Alternate gene names: 158422417

Gene position: 895692-896921 (Clockwise)

Preceding gene: 158422416

Following gene: 158422423

Centisome position: 16.68

GC content: 68.78

Gene sequence:

>1230_bases
ATGTCCAGCCTTGATCCGGCCCGCCTGCGCGCGCTCCTTGCCGAGACCGCCGACGCCCAGCAGGCCTTCCTCGCCGAGCT
GGTGAAGGTGCCCTCCGACAATCCGCCCGGCGACTGCGCGCCCCACGCCCGGCGCGCCGCCGAGCTGCTGGAGGGACTCG
GTTTCGAGGTGGAGCGCCATGTGGTGCCGCAGGACAAGGTGACCGCCGCCGGCATGGTGAGCGTGACCAATCTCATCGTG
CGCCACCGTTTCGGCGACGGCCCGGTGATCGCGCTCAACGCCCACGGCGACGTGGTGCCCCCCGGCGAGGGCTGGTCGCA
GGATCCCTATGGCGCGGCGGTGGTGGACGGCGTGATGTACGGGCGGGGCGTTGCCGTCTCCAAGTCCGATTTCGCCACCT
ACACCTTCGCCCTCGACGCGCTGAAGCGCTCCGGCGTGCCGCTCAAGGGCACGGTGGAACTGCATTTCACCTATGACGAG
GAGATCGGGGGCGAGGTCGGTCCCGGCTGGATCATCTCCGAGAAGCTGACGAAGCCAGATGCGGTGATCTCCGCCGGCTT
CGCGTACGCCGTGGTGGTGGCGCACAACGGCTGTTTGCACCTTGAGGTGGAGGTGAAGGGCAAGTCCGCCCACGCGGCGC
TGCCGCACACCGGCATCGACGCACTCGCCGCCACCACGAAGGTGCTGGAGGCCATCTACGCCTATCGCGACACGCTGAAG
GCCACCGTCTCCCCCACCAAGGGCATCGGCTCGCCGCAGATCACGGTGGGTCTCATCTCGGGCGGCATCAACACCAATGT
GGTGCCCGACCGCATCACCTTACGCCTCGACCGCCGCATCATTCCGCAGGAGCAGCCGGAGGCGGTGGAAGCGGAAGTGA
AGATGCTGATCGAGAAGGCCGTCGCGTCCTACCCCGGCGCGTCCGTCGATATCCGCCGCATCCTGCTTGCCCGGCCGCTG
GTGCCGGATGCGGAGAGCGAGCGCATCGCCGGCATCCTCTGCCGCAACGCTTCCGAGGTGGTGGGCGAGTCGGTCCTGAC
CAACGGCGTGCCGCTCTATACCGATGCGCGGCTTTATGCCGAGGCGGGCATTCCGGTGGTGCTCTATGGCGCCGGCCCCC
GCACCATCGAGGAAGCCAATGGCCACCGCGCCGACGAGAAGCTGCGCCTCTCCGACCTGCGCCTCGCCACCGAGGTGGTG
ACGCGCACGCTGGCCGAACTGCTCGCCTGA

Upstream 100 bases:

>100_bases
CCCTGAGCCGCCGCCCGAACCTTGCCGCTTGACCTCGCCGACGCGGCGGGCCACCCACCCCTGACCGAACACCCGTTCCA
GAAGAAAGAGACCTTGTCCC

Downstream 100 bases:

>100_bases
GCCCTTCCGGGCGGAGCGCGTCTCCGCCCTTTGCGCACGGTGTCAGTCCTAAAATTGCAAACGCCCCGGATCGGCCGATC
CGGGGCGTTTTTATTGGCGT

Product: acetylornithine deacetylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSSLDPARLRALLAETADAQQAFLAELVKVPSDNPPGDCAPHARRAAELLEGLGFEVERHVVPQDKVTAAGMVSVTNLIV
RHRFGDGPVIALNAHGDVVPPGEGWSQDPYGAAVVDGVMYGRGVAVSKSDFATYTFALDALKRSGVPLKGTVELHFTYDE
EIGGEVGPGWIISEKLTKPDAVISAGFAYAVVVAHNGCLHLEVEVKGKSAHAALPHTGIDALAATTKVLEAIYAYRDTLK
ATVSPTKGIGSPQITVGLISGGINTNVVPDRITLRLDRRIIPQEQPEAVEAEVKMLIEKAVASYPGASVDIRRILLARPL
VPDAESERIAGILCRNASEVVGESVLTNGVPLYTDARLYAEAGIPVVLYGAGPRTIEEANGHRADEKLRLSDLRLATEVV
TRTLAELLA

Sequences:

>Translated_409_residues
MSSLDPARLRALLAETADAQQAFLAELVKVPSDNPPGDCAPHARRAAELLEGLGFEVERHVVPQDKVTAAGMVSVTNLIV
RHRFGDGPVIALNAHGDVVPPGEGWSQDPYGAAVVDGVMYGRGVAVSKSDFATYTFALDALKRSGVPLKGTVELHFTYDE
EIGGEVGPGWIISEKLTKPDAVISAGFAYAVVVAHNGCLHLEVEVKGKSAHAALPHTGIDALAATTKVLEAIYAYRDTLK
ATVSPTKGIGSPQITVGLISGGINTNVVPDRITLRLDRRIIPQEQPEAVEAEVKMLIEKAVASYPGASVDIRRILLARPL
VPDAESERIAGILCRNASEVVGESVLTNGVPLYTDARLYAEAGIPVVLYGAGPRTIEEANGHRADEKLRLSDLRLATEVV
TRTLAELLA
>Mature_408_residues
SSLDPARLRALLAETADAQQAFLAELVKVPSDNPPGDCAPHARRAAELLEGLGFEVERHVVPQDKVTAAGMVSVTNLIVR
HRFGDGPVIALNAHGDVVPPGEGWSQDPYGAAVVDGVMYGRGVAVSKSDFATYTFALDALKRSGVPLKGTVELHFTYDEE
IGGEVGPGWIISEKLTKPDAVISAGFAYAVVVAHNGCLHLEVEVKGKSAHAALPHTGIDALAATTKVLEAIYAYRDTLKA
TVSPTKGIGSPQITVGLISGGINTNVVPDRITLRLDRRIIPQEQPEAVEAEVKMLIEKAVASYPGASVDIRRILLARPLV
PDAESERIAGILCRNASEVVGESVLTNGVPLYTDARLYAEAGIPVVLYGAGPRTIEEANGHRADEKLRLSDLRLATEVVT
RTLAELLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501901, Length=337, Percent_Identity=24.6290801186944, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1790395, Length=370, Percent_Identity=26.4864864864865, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788816, Length=401, Percent_Identity=24.1895261845387, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789236, Length=401, Percent_Identity=24.1895261845387, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24649206, Length=409, Percent_Identity=26.4058679706601, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24649202, Length=337, Percent_Identity=27.0029673590504, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24649204, Length=337, Percent_Identity=27.0029673590504, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356353, Length=300, Percent_Identity=27, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24649208, Length=338, Percent_Identity=25.7396449704142, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24649210, Length=340, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24649212, Length=339, Percent_Identity=26.2536873156342, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 43385; Mature: 43254

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLDPARLRALLAETADAQQAFLAELVKVPSDNPPGDCAPHARRAAELLEGLGFEVERH
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEC
VVPQDKVTAAGMVSVTNLIVRHRFGDGPVIALNAHGDVVPPGEGWSQDPYGAAVVDGVMY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
GRGVAVSKSDFATYTFALDALKRSGVPLKGTVELHFTYDEEIGGEVGPGWIISEKLTKPD
CCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECHHCCCCCCCCEEEECCCCCCH
AVISAGFAYAVVVAHNGCLHLEVEVKGKSAHAALPHTGIDALAATTKVLEAIYAYRDTLK
HHHHCCCEEEEEEEECCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ATVSPTKGIGSPQITVGLISGGINTNVVPDRITLRLDRRIIPQEQPEAVEAEVKMLIEKA
EECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
VASYPGASVDIRRILLARPLVPDAESERIAGILCRNASEVVGESVLTNGVPLYTDARLYA
HHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCEEECCEEEE
EAGIPVVLYGAGPRTIEEANGHRADEKLRLSDLRLATEVVTRTLAELLA
CCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SSLDPARLRALLAETADAQQAFLAELVKVPSDNPPGDCAPHARRAAELLEGLGFEVERH
CCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEC
VVPQDKVTAAGMVSVTNLIVRHRFGDGPVIALNAHGDVVPPGEGWSQDPYGAAVVDGVMY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
GRGVAVSKSDFATYTFALDALKRSGVPLKGTVELHFTYDEEIGGEVGPGWIISEKLTKPD
CCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECHHCCCCCCCCEEEECCCCCCH
AVISAGFAYAVVVAHNGCLHLEVEVKGKSAHAALPHTGIDALAATTKVLEAIYAYRDTLK
HHHHCCCEEEEEEEECCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ATVSPTKGIGSPQITVGLISGGINTNVVPDRITLRLDRRIIPQEQPEAVEAEVKMLIEKA
EECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
VASYPGASVDIRRILLARPLVPDAESERIAGILCRNASEVVGESVLTNGVPLYTDARLYA
HHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCEEECCEEEE
EAGIPVVLYGAGPRTIEEANGHRADEKLRLSDLRLATEVVTRTLAELLA
CCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA