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The map label for this gene is cysU [H]

Identifier: 158422407

GI number: 158422407

Start: 884905

End: 885732

Strand: Direct

Name: cysU [H]

Synonym: AZC_0783

Alternate gene names: 158422407

Gene position: 884905-885732 (Clockwise)

Preceding gene: 158422406

Following gene: 158422408

Centisome position: 16.48

GC content: 68.36

Gene sequence:

>828_bases
GTGAAAGCCATGCCCAGCGCAGATCGTCTGGCCCGTCTGTTCCTCTTGCCGCTCGGCCTGTTCGTGCTGGCCTTCTTCCT
GCTGCCCATGGCGCGGCTCGTGGCCGTCGCGGGCTCGGGTCCCAACGGGTTGGGCGAATATCTGGTGCTTGTCACCAGCC
CGCGCCATTTCTCCACTCTCATCGCGACGCTGGTCCTTTCCGCCACCGCGACGCTGGCGACGCTCGCCATCGGCGTCGTG
GTGGCCCTGTTCCTCGGTCGCACGCGCTTTCCGGGGCAGAACATCCTGATCGCAATGCTCACCTTGCCGCTCGCCTTTCC
CGGCGTGGTGGTGGGCTTCATGATCATCCTGCTCGCCGGCCGGCAGGGCCTCATCGGCGGCTGGACGCAGGCGCTCGGCA
TGGGGCGGCTGGTGTTCGCCTATTCCATGGCCGGCCTGTTCGCGGGCTATGTCTATTTCTCGCTGCCGCGGGTGATGGTG
ACGCTGCTCGCCGCGGTGGAGAAGCTCGATCCGGCGCTGGAGGAGGCCGCCCGCTCGCTCGGCGCGAGTCGGGTGCAGGT
GCTGCGGGATGTGATCCTGCCCGGCATCGCGCCCTCGCTCATGGCCTCCGGCGCCATCGCCTTCGCCACCGCCATGGGCG
CCTTCGGCACCGCATTTACGCTCGCCACCCGCATCGATGTGCTGGCCATGACCATCTATACGGAGTTTACGCTCAACGCG
AACATCGCCAGCGCGGCGGCGCTCTCCATCGCGCTCGGCCTCATCACCTGGCTGGTGCTGGCGGCGACACGCAATCTCGC
GGGTGACCGCGTGGCGGCGGCGGGGTGA

Upstream 100 bases:

>100_bases
TGTGGGCTGCCCAGCGTATCCGCGACCGCCTGCCGCCGCCTTCCCTTGAGCAAGCCTTCAACCCGTCCGCCGGCGTGCCG
CGCGCCGGCACCTTCGCAAA

Downstream 100 bases:

>100_bases
GGCCGATGAAACGCTCCCCTCTCGTTTTCGCCCTGCAACTCGTTATCACGCTGCTCGCCACGGCCTTCCTGGTGGTGCCG
GCGGGCACCTCCATCCTTGC

Product: permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKAMPSADRLARLFLLPLGLFVLAFFLLPMARLVAVAGSGPNGLGEYLVLVTSPRHFSTLIATLVLSATATLATLAIGVV
VALFLGRTRFPGQNILIAMLTLPLAFPGVVVGFMIILLAGRQGLIGGWTQALGMGRLVFAYSMAGLFAGYVYFSLPRVMV
TLLAAVEKLDPALEEAARSLGASRVQVLRDVILPGIAPSLMASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNA
NIASAAALSIALGLITWLVLAATRNLAGDRVAAAG

Sequences:

>Translated_275_residues
MKAMPSADRLARLFLLPLGLFVLAFFLLPMARLVAVAGSGPNGLGEYLVLVTSPRHFSTLIATLVLSATATLATLAIGVV
VALFLGRTRFPGQNILIAMLTLPLAFPGVVVGFMIILLAGRQGLIGGWTQALGMGRLVFAYSMAGLFAGYVYFSLPRVMV
TLLAAVEKLDPALEEAARSLGASRVQVLRDVILPGIAPSLMASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNA
NIASAAALSIALGLITWLVLAATRNLAGDRVAAAG
>Mature_275_residues
MKAMPSADRLARLFLLPLGLFVLAFFLLPMARLVAVAGSGPNGLGEYLVLVTSPRHFSTLIATLVLSATATLATLAIGVV
VALFLGRTRFPGQNILIAMLTLPLAFPGVVVGFMIILLAGRQGLIGGWTQALGMGRLVFAYSMAGLFAGYVYFSLPRVMV
TLLAAVEKLDPALEEAARSLGASRVQVLRDVILPGIAPSLMASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNA
NIASAAALSIALGLITWLVLAATRNLAGDRVAAAG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=192, Percent_Identity=31.25, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082099, Length=206, Percent_Identity=31.0679611650485, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786980, Length=229, Percent_Identity=31.8777292576419, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787369, Length=167, Percent_Identity=29.940119760479, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786254, Length=190, Percent_Identity=27.3684210526316, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787570, Length=242, Percent_Identity=27.6859504132231, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787368, Length=203, Percent_Identity=27.5862068965517, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28568; Mature: 28568

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAMPSADRLARLFLLPLGLFVLAFFLLPMARLVAVAGSGPNGLGEYLVLVTSPRHFSTL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHH
IATLVLSATATLATLAIGVVVALFLGRTRFPGQNILIAMLTLPLAFPGVVVGFMIILLAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RQGLIGGWTQALGMGRLVFAYSMAGLFAGYVYFSLPRVMVTLLAAVEKLDPALEEAARSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
GASRVQVLRDVILPGIAPSLMASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
NIASAAALSIALGLITWLVLAATRNLAGDRVAAAG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAMPSADRLARLFLLPLGLFVLAFFLLPMARLVAVAGSGPNGLGEYLVLVTSPRHFSTL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHH
IATLVLSATATLATLAIGVVVALFLGRTRFPGQNILIAMLTLPLAFPGVVVGFMIILLAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RQGLIGGWTQALGMGRLVFAYSMAGLFAGYVYFSLPRVMVTLLAAVEKLDPALEEAARSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
GASRVQVLRDVILPGIAPSLMASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
NIASAAALSIALGLITWLVLAATRNLAGDRVAAAG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]