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The map label for this gene is lacI [H]

Identifier: 158422405

GI number: 158422405

Start: 882494

End: 883588

Strand: Direct

Name: lacI [H]

Synonym: AZC_0781

Alternate gene names: 158422405

Gene position: 882494-883588 (Clockwise)

Preceding gene: 158422404

Following gene: 158422406

Centisome position: 16.43

GC content: 71.96

Gene sequence:

>1095_bases
GTGCCCCAGACCGTCACCATACGCACCGTCGCCGAACATGCCGGCTGCTCCATCGCGACCGTCAGCCGCGTGCTGAACGG
GACCGCGCGGACCAGCGCGGAGGTGGAGGCCCGCGTCCGGGCGGCGGTAGCGGAACTCGGCTTCCGGCCCAGCGAGATCG
GGCGTTCCTTGAAGACGCTGCGCACGCGCACGCTCGGCGTCGTCATTCCGAGCCTCACCAATCCGGTTTTCGCGGGCTCG
GTGGCGGGCATGGAGGCCGAAGCGCGCCACCGTGCCCACACCCTGCTGCTGACGGCCACCGACTATGATGCCGAGCGCGA
ATGGGAACTGGTCGGCACGCTGGTGGCCCAGCGGGTGGCGGGCCTCGTGCTCACCGTTGCCGATCCGGACAACAACGCCA
CGCTCGATTGGCTCGATGCGGAGCGCATCCCCTATGTGCTCGTCTATAACGAGCCACAGGGCGCGAGCCGCGCGTCGGTC
ACCGTGGACAATGCGGGTGCCAGCCGCGCGCTGACGCAGGCCTTCCTCGCCGCCGGCCACCGGCGCCTGCTGTTCATCGC
CGGGCGCTTCTCCACCTCGGACCGCTCGCGCCTGCGCCATCAGGGCTATCTCGCCGCCATGGCCGCTGCGGGCCTGGAGC
CCGAGCCCGTTCTGGAACTCGACTATCTCGCCGACACCGCCGCCCACCGCGCTGCCCTGGCGGGCCTGCTCGCGGGGCCG
GACCGGCCGACGGGCATCATCTGCTCCAACGATCTGCTCGCCCTTTCGGTCATCGCCGCCGTGCGCGAGATGGGGCTCGC
CGTGCCGCAGGATGTCTCGGTCGCCGGTTTCGACGGCATCGCCATCGGTCGCCTCGTCACGCCGGTGCTGGCGACCGTGG
ACCAACCCACCCGCCGCATGGGCGAGCGGGCAGTGGCGCGGCTGTTCGACCTCATCGAGGGCGAGCGCGCGCGCACCGTG
GAGCATCTGCCGTTCGAACTGCGCGCCGCCGGCACGCTCGCCCCCGCGCCGCATCTGCCGCCGGCCGCCCCCAAGGGGCG
GCGCGCCGCTACCCCTTCACCCCGCCAAACCGCCTCAGGAAAGGCTCTGCGATGA

Upstream 100 bases:

>100_bases
GGCGGCGCCTTTTTCTTTGCCGGGCAGGCATCGGCGCGTGTCACAGGGCTGTTGCAGAGGCGCGATAGGAGAGCCGTGAA
AACGATTTCACGGATCCCTC

Downstream 100 bases:

>100_bases
AGTCCCTGCTCACGACCGTTCTGCTCGCCACCGGGCTTTCGCTCGGCGCGGCGTCGGCCGCTTCGGCCGCCGATGCCATC
TGCTACAACTGCCCGCCCGA

Product: regulatory protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MPQTVTIRTVAEHAGCSIATVSRVLNGTARTSAEVEARVRAAVAELGFRPSEIGRSLKTLRTRTLGVVIPSLTNPVFAGS
VAGMEAEARHRAHTLLLTATDYDAEREWELVGTLVAQRVAGLVLTVADPDNNATLDWLDAERIPYVLVYNEPQGASRASV
TVDNAGASRALTQAFLAAGHRRLLFIAGRFSTSDRSRLRHQGYLAAMAAAGLEPEPVLELDYLADTAAHRAALAGLLAGP
DRPTGIICSNDLLALSVIAAVREMGLAVPQDVSVAGFDGIAIGRLVTPVLATVDQPTRRMGERAVARLFDLIEGERARTV
EHLPFELRAAGTLAPAPHLPPAAPKGRRAATPSPRQTASGKALR

Sequences:

>Translated_364_residues
MPQTVTIRTVAEHAGCSIATVSRVLNGTARTSAEVEARVRAAVAELGFRPSEIGRSLKTLRTRTLGVVIPSLTNPVFAGS
VAGMEAEARHRAHTLLLTATDYDAEREWELVGTLVAQRVAGLVLTVADPDNNATLDWLDAERIPYVLVYNEPQGASRASV
TVDNAGASRALTQAFLAAGHRRLLFIAGRFSTSDRSRLRHQGYLAAMAAAGLEPEPVLELDYLADTAAHRAALAGLLAGP
DRPTGIICSNDLLALSVIAAVREMGLAVPQDVSVAGFDGIAIGRLVTPVLATVDQPTRRMGERAVARLFDLIEGERARTV
EHLPFELRAAGTLAPAPHLPPAAPKGRRAATPSPRQTASGKALR
>Mature_363_residues
PQTVTIRTVAEHAGCSIATVSRVLNGTARTSAEVEARVRAAVAELGFRPSEIGRSLKTLRTRTLGVVIPSLTNPVFAGSV
AGMEAEARHRAHTLLLTATDYDAEREWELVGTLVAQRVAGLVLTVADPDNNATLDWLDAERIPYVLVYNEPQGASRASVT
VDNAGASRALTQAFLAAGHRRLLFIAGRFSTSDRSRLRHQGYLAAMAAAGLEPEPVLELDYLADTAAHRAALAGLLAGPD
RPTGIICSNDLLALSVIAAVREMGLAVPQDVSVAGFDGIAIGRLVTPVLATVDQPTRRMGERAVARLFDLIEGERARTVE
HLPFELRAAGTLAPAPHLPPAAPKGRRAATPSPRQTASGKALR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=32.5732899022801, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=31.0897435897436, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790369, Length=314, Percent_Identity=27.7070063694268, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787948, Length=313, Percent_Identity=30.3514376996805, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=29.5681063122924, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790715, Length=338, Percent_Identity=28.698224852071, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789068, Length=321, Percent_Identity=31.7757009345794, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=29.3918918918919, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=28.8288288288288, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=27.3584905660377, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787906, Length=293, Percent_Identity=28.3276450511945, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790689, Length=314, Percent_Identity=24.8407643312102, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786268, Length=309, Percent_Identity=24.5954692556634, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38550; Mature: 38418

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTVTIRTVAEHAGCSIATVSRVLNGTARTSAEVEARVRAAVAELGFRPSEIGRSLKTL
CCCEEEEEEHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RTRTLGVVIPSLTNPVFAGSVAGMEAEARHRAHTLLLTATDYDAEREWELVGTLVAQRVA
HHHHHEEEECCCCCCHHCCCCCCCCHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHHHHC
GLVLTVADPDNNATLDWLDAERIPYVLVYNEPQGASRASVTVDNAGASRALTQAFLAAGH
CEEEEEECCCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCC
RRLLFIAGRFSTSDRSRLRHQGYLAAMAAAGLEPEPVLELDYLADTAAHRAALAGLLAGP
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCC
DRPTGIICSNDLLALSVIAAVREMGLAVPQDVSVAGFDGIAIGRLVTPVLATVDQPTRRM
CCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
GERAVARLFDLIEGERARTVEHLPFELRAAGTLAPAPHLPPAAPKGRRAATPSPRQTASG
HHHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KALR
CCCC
>Mature Secondary Structure 
PQTVTIRTVAEHAGCSIATVSRVLNGTARTSAEVEARVRAAVAELGFRPSEIGRSLKTL
CCEEEEEEHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RTRTLGVVIPSLTNPVFAGSVAGMEAEARHRAHTLLLTATDYDAEREWELVGTLVAQRVA
HHHHHEEEECCCCCCHHCCCCCCCCHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHHHHC
GLVLTVADPDNNATLDWLDAERIPYVLVYNEPQGASRASVTVDNAGASRALTQAFLAAGH
CEEEEEECCCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCC
RRLLFIAGRFSTSDRSRLRHQGYLAAMAAAGLEPEPVLELDYLADTAAHRAALAGLLAGP
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCC
DRPTGIICSNDLLALSVIAAVREMGLAVPQDVSVAGFDGIAIGRLVTPVLATVDQPTRRM
CCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
GERAVARLFDLIEGERARTVEHLPFELRAAGTLAPAPHLPPAAPKGRRAATPSPRQTASG
HHHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KALR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA