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The map label for this gene is gatA [H]

Identifier: 158422345

GI number: 158422345

Start: 806102

End: 807523

Strand: Direct

Name: gatA [H]

Synonym: AZC_0721

Alternate gene names: 158422345

Gene position: 806102-807523 (Clockwise)

Preceding gene: 158422344

Following gene: 158422347

Centisome position: 15.01

GC content: 71.03

Gene sequence:

>1422_bases
GTGAGCGCGCTCCACGACCTCGGGCTCGTCGAGACGGCGGACGCCATCGCGCAGGGCGCCATCACCTCCGAAGCCGCGAC
CGCCTCTGCGCTGCACCGGCTGGAAGCCCTCGGCCCGCGCTACCACGCTGTGATGCTCATTGACCGCGACGGCGCGCTGG
AAGCCGCGCGCGCGGTGGACCTTGCCCGCGCGCGGGGCGAGGACATCGGCCCGCTCGGCGGCGTGCCGCTGGCGCACAAG
GATCTGCTCTATCGTGCGGGCCGCGTCTGCACCGGCGGCTCGCTCATCCGCAAGGACTTCGTCCCCGACGTGACCGCCTC
CGTGCTGGAGCGGCTCGACGGGGCGGGGGCGCTTGATCTCGGCACGCTGCATCTCGCCGAATTCGCCCTGTCGCCCACCG
GCTTCAATGCCCATTACGGCCATGGCCGCAGCCCGTGGAACCTCGATTACGGCTCCGGCGGTTCGTCCTCCGGCTCCGGT
GCGGCGGTGGCGGCGCGGCTGGTGAGCGGCGCGCTCGGCTCCGACACCGGCGGCTCCATCCGCCATCCCTCGGCCATGTG
CGGCGTTACGGGCCTGAAGCCCACCAACGGCCTCGTGCCGCTTTATGGCGCCATGCCCATGGCGCCGAGCCTCGACACCA
TCGGCCCGCTTACCCGCTCCGTGCGCGATGCGGCCCGGATGCTCAGCGTGATCGCCGGGCCGGATGTGCGCGATGCCACG
ACGCTGGCCGCACCCGAGCGCGATTATGAAAGTGGCCTCTCGGGTGATCTGAAGGGCCTGACCATCGCCGTGCCGCAGGG
CTATTACCGCACGACCGCCGATGGCGAGATCCTAGCCCTCATGGACGATAGCCTCAAGGTGCTGAAGGAGGCCGGGGCGA
AGGTGGTGGAGACCACCGTGCCCGACATGGCGCTCATCAATGCCCTCATGCAGGTGGTGATGACCTGCGAGGCTGCCACC
CTGCATCGCCAATGGCTGGAGGAGCGGCCGGGCGATTATGGGGCGCAGGTGCGCGCCCGCATCCTGCCCGGCTTCGCGCT
GCCCGCGACGCGCTATGTGGAAGCACTGATGATGCGGGCCAAGGTGGCGGAGGACTGGCTGCACCGGGCCATGGGCGGCG
CCGACATGGTGCATATCCCGACCCTGCCCATCGCCGTGCCGAGCATTGCGGAGACGACGCAGGGGGCGCCGGAGGACGTG
GCGGTGGCCATCGCCCGCGTCACCCATTGCACGCGGGCGATCAACTATCTCGGCCTGCCCTCGCTCTCCGTGCCCTGCGG
CTTCACCGCCAACGGCCTGCCGGCCGCCTTCCAGCTGGTCGGCCGTCCTTTCGGCGAGCCGGTGCTGCTCCGGGCGGGCG
ACGCCTATCAGCGGCGCACCGACTTCCACCTGCGCGTGCCGCCCGATTGCGGGCCGATCTGA

Upstream 100 bases:

>100_bases
CCGTGGCCGCAGGCGCGAAGGCGCGCGTCGATATCGACGGCTGCCCGTGGAGCTACGCCAACCTCCTCGCCGCCGTGGCC
GAGCGCGGGGAGGGCGAGGC

Downstream 100 bases:

>100_bases
GGCGGGGGAGCGCCCGGCTCTTCGATGATCCGAAGGCGGCGGGTGGCCCGCCGCCTTCTCGCGCCTCAGCGGAAGAGGTT
GCGCTTGGCGAGTTCGAGCA

Product: glutamyl-tRNA(Gln) amidotransferase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSALHDLGLVETADAIAQGAITSEAATASALHRLEALGPRYHAVMLIDRDGALEAARAVDLARARGEDIGPLGGVPLAHK
DLLYRAGRVCTGGSLIRKDFVPDVTASVLERLDGAGALDLGTLHLAEFALSPTGFNAHYGHGRSPWNLDYGSGGSSSGSG
AAVAARLVSGALGSDTGGSIRHPSAMCGVTGLKPTNGLVPLYGAMPMAPSLDTIGPLTRSVRDAARMLSVIAGPDVRDAT
TLAAPERDYESGLSGDLKGLTIAVPQGYYRTTADGEILALMDDSLKVLKEAGAKVVETTVPDMALINALMQVVMTCEAAT
LHRQWLEERPGDYGAQVRARILPGFALPATRYVEALMMRAKVAEDWLHRAMGGADMVHIPTLPIAVPSIAETTQGAPEDV
AVAIARVTHCTRAINYLGLPSLSVPCGFTANGLPAAFQLVGRPFGEPVLLRAGDAYQRRTDFHLRVPPDCGPI

Sequences:

>Translated_473_residues
MSALHDLGLVETADAIAQGAITSEAATASALHRLEALGPRYHAVMLIDRDGALEAARAVDLARARGEDIGPLGGVPLAHK
DLLYRAGRVCTGGSLIRKDFVPDVTASVLERLDGAGALDLGTLHLAEFALSPTGFNAHYGHGRSPWNLDYGSGGSSSGSG
AAVAARLVSGALGSDTGGSIRHPSAMCGVTGLKPTNGLVPLYGAMPMAPSLDTIGPLTRSVRDAARMLSVIAGPDVRDAT
TLAAPERDYESGLSGDLKGLTIAVPQGYYRTTADGEILALMDDSLKVLKEAGAKVVETTVPDMALINALMQVVMTCEAAT
LHRQWLEERPGDYGAQVRARILPGFALPATRYVEALMMRAKVAEDWLHRAMGGADMVHIPTLPIAVPSIAETTQGAPEDV
AVAIARVTHCTRAINYLGLPSLSVPCGFTANGLPAAFQLVGRPFGEPVLLRAGDAYQRRTDFHLRVPPDCGPI
>Mature_472_residues
SALHDLGLVETADAIAQGAITSEAATASALHRLEALGPRYHAVMLIDRDGALEAARAVDLARARGEDIGPLGGVPLAHKD
LLYRAGRVCTGGSLIRKDFVPDVTASVLERLDGAGALDLGTLHLAEFALSPTGFNAHYGHGRSPWNLDYGSGGSSSGSGA
AVAARLVSGALGSDTGGSIRHPSAMCGVTGLKPTNGLVPLYGAMPMAPSLDTIGPLTRSVRDAARMLSVIAGPDVRDATT
LAAPERDYESGLSGDLKGLTIAVPQGYYRTTADGEILALMDDSLKVLKEAGAKVVETTVPDMALINALMQVVMTCEAATL
HRQWLEERPGDYGAQVRARILPGFALPATRYVEALMMRAKVAEDWLHRAMGGADMVHIPTLPIAVPSIAETTQGAPEDVA
VAIARVTHCTRAINYLGLPSLSVPCGFTANGLPAAFQLVGRPFGEPVLLRAGDAYQRRTDFHLRVPPDCGPI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=29.1417165668663, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI195972892, Length=484, Percent_Identity=26.0330578512397, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17543272, Length=450, Percent_Identity=26.8888888888889, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17537465, Length=239, Percent_Identity=34.7280334728033, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=460, Percent_Identity=23.4782608695652, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=395, Percent_Identity=22.7848101265823, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=306, Percent_Identity=24.8366013071895, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556276, Length=311, Percent_Identity=24.1157556270096, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=260, Percent_Identity=26.5384615384615, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=28.030303030303, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=413, Percent_Identity=24.455205811138, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648113, Length=437, Percent_Identity=31.350114416476, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=23.9130434782609, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356731, Length=240, Percent_Identity=32.0833333333333, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550774, Length=245, Percent_Identity=28.1632653061224, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648435, Length=245, Percent_Identity=28.1632653061224, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648437, Length=245, Percent_Identity=28.1632653061224, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI161078093, Length=502, Percent_Identity=23.7051792828685, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24652985, Length=244, Percent_Identity=28.2786885245902, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922090, Length=244, Percent_Identity=28.2786885245902, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652981, Length=244, Percent_Identity=28.2786885245902, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652983, Length=244, Percent_Identity=28.2786885245902, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648441, Length=173, Percent_Identity=28.9017341040462, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648439, Length=173, Percent_Identity=28.9017341040462, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49440; Mature: 49309

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALHDLGLVETADAIAQGAITSEAATASALHRLEALGPRYHAVMLIDRDGALEAARAVD
CCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHH
LARARGEDIGPLGGVPLAHKDLLYRAGRVCTGGSLIRKDFVPDVTASVLERLDGAGALDL
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCH
GTLHLAEFALSPTGFNAHYGHGRSPWNLDYGSGGSSSGSGAAVAARLVSGALGSDTGGSI
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
RHPSAMCGVTGLKPTNGLVPLYGAMPMAPSLDTIGPLTRSVRDAARMLSVIAGPDVRDAT
CCCHHHHCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
TLAAPERDYESGLSGDLKGLTIAVPQGYYRTTADGEILALMDDSLKVLKEAGAKVVETTV
HHCCCCCHHHCCCCCCCCCEEEEECCCCEEECCCCCEEEEEHHHHHHHHHCCCCEEHHCC
PDMALINALMQVVMTCEAATLHRQWLEERPGDYGAQVRARILPGFALPATRYVEALMMRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
KVAEDWLHRAMGGADMVHIPTLPIAVPSIAETTQGAPEDVAVAIARVTHCTRAINYLGLP
HHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SLSVPCGFTANGLPAAFQLVGRPFGEPVLLRAGDAYQRRTDFHLRVPPDCGPI
CCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCHHHHCCCCEEECCCCCCCC
>Mature Secondary Structure 
SALHDLGLVETADAIAQGAITSEAATASALHRLEALGPRYHAVMLIDRDGALEAARAVD
CCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHH
LARARGEDIGPLGGVPLAHKDLLYRAGRVCTGGSLIRKDFVPDVTASVLERLDGAGALDL
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCH
GTLHLAEFALSPTGFNAHYGHGRSPWNLDYGSGGSSSGSGAAVAARLVSGALGSDTGGSI
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
RHPSAMCGVTGLKPTNGLVPLYGAMPMAPSLDTIGPLTRSVRDAARMLSVIAGPDVRDAT
CCCHHHHCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
TLAAPERDYESGLSGDLKGLTIAVPQGYYRTTADGEILALMDDSLKVLKEAGAKVVETTV
HHCCCCCHHHCCCCCCCCCEEEEECCCCEEECCCCCEEEEEHHHHHHHHHCCCCEEHHCC
PDMALINALMQVVMTCEAATLHRQWLEERPGDYGAQVRARILPGFALPATRYVEALMMRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
KVAEDWLHRAMGGADMVHIPTLPIAVPSIAETTQGAPEDVAVAIARVTHCTRAINYLGLP
HHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SLSVPCGFTANGLPAAFQLVGRPFGEPVLLRAGDAYQRRTDFHLRVPPDCGPI
CCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCHHHHCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA