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The map label for this gene is fixL [H]

Identifier: 158422239

GI number: 158422239

Start: 686732

End: 688432

Strand: Direct

Name: fixL [H]

Synonym: AZC_0615

Alternate gene names: 158422239

Gene position: 686732-688432 (Clockwise)

Preceding gene: 158422238

Following gene: 158422240

Centisome position: 12.79

GC content: 66.14

Gene sequence:

>1701_bases
ATGCCGGCTGCGCTGATCGTGACGACGCTCGGCCTCTTCCTGGCGACGGGGATGCTGTATGTGACGCAGACGCAGCATCG
CGCGCTGCTCTCGGCCACCGTGCGCTCCTCCGGCTGGGTGGCCTATCAGGCCCAGCTCGAGTTCGTGAAGACGCAGGGCC
AGTTCGAAATCGCCATGCTGCGCCCCAGCCGCCGCGCGCTGGACGAGCTTCAGTTCCGACTGGAGCTCCTGCGCTCGCGG
TTGCCGTTGCTCTATGATTCCGACGAAGGGCGGCTGCTTCAGGAAGTGGCCGCCCAGAAGGATGTGCTGCAGGCCTTCGA
GCGAACGCTGGATGCCACCATCGATTCCCTGCCGGACACCTCGCCCTCGGCGCCCGAGACGGTGGCGCGTCTGCGGGGGG
CCTATTACGAGCTCGAGCCACTGGGGCGGGTGCTCCAGAAAGTGCTGATGCAGTCCATCGCCTATAATGAGGAGATATTC
CGACGCGAGCGGGAGGTCGCGGCAAGCCCGGCTATCGTGCCCTTTGCCCTGCTGTTCGTCTCCGGCACCTGCCTTGTCGC
CATCCTCTTCATGGACGGTCGGCGCGACCGGCAGCGTCTTGCGGACGTCACGGCCGCCAAGGCCGAGGTGACGGCGGTGC
AGGACAATCTGCGCGCGATCATCGAGGCCATGCCCATCGGCACCATCATCGTCGATCCCGACGGCGATCAGGTGCGCTTC
ATCAATCCGAGCGCGGCCGCGCTTGTGGACCCGTCTCCCGATCATCCGGAATGGCGGCGGCTGATCCGCGCCGCGTTCGA
GGCGGCGATCGACGAGAACGGCCGCAAGACCGGGACGCTGTCCATGGCGTTCGCCAAGCCGCTCGGCGACATCGTCTCCC
TGCGCGGCGCGGTGTGCGGCGTGATCTGGGAAGGGCGCCCCCAGATCCTCATCCTGCTGACCGACACGAGCCGGGTGCGC
AATGCCGAGTTGCAACTGATGCAGGCGGCCAAGCTCGCGACCCTGGGCGAGATGGCCACCGCCATCGCCCATGAGCTCAA
CCAGCCGCTCGCGGTGATCAAGATGGCGGTCGCCAATGCCCGCCGGCTGGTGCTGAGCGAAGCGGATCCGGAGGCCATCA
CCAGCAAGCTCGATCGCATCGGTGCGCAGGTGGACCGGGCGAAACGCATCACCGATCAGGTGCGGCGCTATGCCCGCATG
CCCGGCGATCAGACCGCCTCCTTCGTATTGCGGCAGGCGGTTGAGCTGGCGGCGGGCTTCGTGGCCGAGCAATATCGCGC
CGCGGGCATCCGCATGGTGCTCGATCTCGAGGTCTCCCCGGAGCTTCTGGTGAGTGGCGACCAGACCATGTTCGAGCAGG
TGATCGTGAACCTGCTGATCAATTCGCGCGATGCCTACGACAGCCAGCGGCAGGATACGCACCAGCCACAGGTGGTGGTG
CGTGCGGTGGTGCGGGGCGGCGAGGTGGTGATCGAGGTGACGGACGATGCGGGCGGCATCCGGAATGATATTCTGGGCAA
CATCTTCGATCCCTTCTGCACCACAAAATCGGCGGAAAAGGGCACTGGTCTCGGTCTCTCCATGGCGCGCAATGTGGTGC
GTGACATGAACGGGACCATTGCTGCCGAGAACGTGGAGGGCGGAGCGCGCTTCACCATCGTTCTGCCGGCGGCCCGCACG
CAAGCCCAGGATGTTGCCTGA

Upstream 100 bases:

>100_bases
CGGGATGTCGATACGCAAATGTATCAGGCCCGCATGATGTGGCAGGTCAAATCTCTTGAATTCGTCGTTGAATAATCGAT
CCCGCTCGTGGTCCAGGCTG

Downstream 100 bases:

>100_bases
TGCCGGGTTATTCGATTCTGATCTGTGACGACGAACAGGAGCTGGCCGAGGAACTGGGGGAGTTTTTCAGCTCATTGGGG
TGGAACGTGCGTGTGTGCCT

Product: sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MPAALIVTTLGLFLATGMLYVTQTQHRALLSATVRSSGWVAYQAQLEFVKTQGQFEIAMLRPSRRALDELQFRLELLRSR
LPLLYDSDEGRLLQEVAAQKDVLQAFERTLDATIDSLPDTSPSAPETVARLRGAYYELEPLGRVLQKVLMQSIAYNEEIF
RREREVAASPAIVPFALLFVSGTCLVAILFMDGRRDRQRLADVTAAKAEVTAVQDNLRAIIEAMPIGTIIVDPDGDQVRF
INPSAAALVDPSPDHPEWRRLIRAAFEAAIDENGRKTGTLSMAFAKPLGDIVSLRGAVCGVIWEGRPQILILLTDTSRVR
NAELQLMQAAKLATLGEMATAIAHELNQPLAVIKMAVANARRLVLSEADPEAITSKLDRIGAQVDRAKRITDQVRRYARM
PGDQTASFVLRQAVELAAGFVAEQYRAAGIRMVLDLEVSPELLVSGDQTMFEQVIVNLLINSRDAYDSQRQDTHQPQVVV
RAVVRGGEVVIEVTDDAGGIRNDILGNIFDPFCTTKSAEKGTGLGLSMARNVVRDMNGTIAAENVEGGARFTIVLPAART
QAQDVA

Sequences:

>Translated_566_residues
MPAALIVTTLGLFLATGMLYVTQTQHRALLSATVRSSGWVAYQAQLEFVKTQGQFEIAMLRPSRRALDELQFRLELLRSR
LPLLYDSDEGRLLQEVAAQKDVLQAFERTLDATIDSLPDTSPSAPETVARLRGAYYELEPLGRVLQKVLMQSIAYNEEIF
RREREVAASPAIVPFALLFVSGTCLVAILFMDGRRDRQRLADVTAAKAEVTAVQDNLRAIIEAMPIGTIIVDPDGDQVRF
INPSAAALVDPSPDHPEWRRLIRAAFEAAIDENGRKTGTLSMAFAKPLGDIVSLRGAVCGVIWEGRPQILILLTDTSRVR
NAELQLMQAAKLATLGEMATAIAHELNQPLAVIKMAVANARRLVLSEADPEAITSKLDRIGAQVDRAKRITDQVRRYARM
PGDQTASFVLRQAVELAAGFVAEQYRAAGIRMVLDLEVSPELLVSGDQTMFEQVIVNLLINSRDAYDSQRQDTHQPQVVV
RAVVRGGEVVIEVTDDAGGIRNDILGNIFDPFCTTKSAEKGTGLGLSMARNVVRDMNGTIAAENVEGGARFTIVLPAART
QAQDVA
>Mature_565_residues
PAALIVTTLGLFLATGMLYVTQTQHRALLSATVRSSGWVAYQAQLEFVKTQGQFEIAMLRPSRRALDELQFRLELLRSRL
PLLYDSDEGRLLQEVAAQKDVLQAFERTLDATIDSLPDTSPSAPETVARLRGAYYELEPLGRVLQKVLMQSIAYNEEIFR
REREVAASPAIVPFALLFVSGTCLVAILFMDGRRDRQRLADVTAAKAEVTAVQDNLRAIIEAMPIGTIIVDPDGDQVRFI
NPSAAALVDPSPDHPEWRRLIRAAFEAAIDENGRKTGTLSMAFAKPLGDIVSLRGAVCGVIWEGRPQILILLTDTSRVRN
AELQLMQAAKLATLGEMATAIAHELNQPLAVIKMAVANARRLVLSEADPEAITSKLDRIGAQVDRAKRITDQVRRYARMP
GDQTASFVLRQAVELAAGFVAEQYRAAGIRMVLDLEVSPELLVSGDQTMFEQVIVNLLINSRDAYDSQRQDTHQPQVVVR
AVVRGGEVVIEVTDDAGGIRNDILGNIFDPFCTTKSAEKGTGLGLSMARNVVRDMNGTIAAENVEGGARFTIVLPAARTQ
AQDVA

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=253, Percent_Identity=28.8537549407115, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=30.0847457627119, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=30.5309734513274, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=30.7339449541284, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788393, Length=234, Percent_Identity=29.0598290598291, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790300, Length=283, Percent_Identity=26.1484098939929, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61951; Mature: 61820

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAALIVTTLGLFLATGMLYVTQTQHRALLSATVRSSGWVAYQAQLEFVKTQGQFEIAML
CCHHHHHHHHHHHHHCCHHEEECHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCEEEEEE
RPSRRALDELQFRLELLRSRLPLLYDSDEGRLLQEVAAQKDVLQAFERTLDATIDSLPDT
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SPSAPETVARLRGAYYELEPLGRVLQKVLMQSIAYNEEIFRREREVAASPAIVPFALLFV
CCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
SGTCLVAILFMDGRRDRQRLADVTAAKAEVTAVQDNLRAIIEAMPIGTIIVDPDGDQVRF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
INPSAAALVDPSPDHPEWRRLIRAAFEAAIDENGRKTGTLSMAFAKPLGDIVSLRGAVCG
ECCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHEE
VIWEGRPQILILLTDTSRVRNAELQLMQAAKLATLGEMATAIAHELNQPLAVIKMAVANA
EEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RRLVLSEADPEAITSKLDRIGAQVDRAKRITDQVRRYARMPGDQTASFVLRQAVELAAGF
HHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VAEQYRAAGIRMVLDLEVSPELLVSGDQTMFEQVIVNLLINSRDAYDSQRQDTHQPQVVV
HHHHHHHCCEEEEEEECCCCHHEECCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHH
RAVVRGGEVVIEVTDDAGGIRNDILGNIFDPFCTTKSAEKGTGLGLSMARNVVRDMNGTI
HHHHCCCEEEEEECCCCCCHHHHHHHHHHCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCE
AAENVEGGARFTIVLPAARTQAQDVA
EEECCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
PAALIVTTLGLFLATGMLYVTQTQHRALLSATVRSSGWVAYQAQLEFVKTQGQFEIAML
CHHHHHHHHHHHHHCCHHEEECHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCEEEEEE
RPSRRALDELQFRLELLRSRLPLLYDSDEGRLLQEVAAQKDVLQAFERTLDATIDSLPDT
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SPSAPETVARLRGAYYELEPLGRVLQKVLMQSIAYNEEIFRREREVAASPAIVPFALLFV
CCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
SGTCLVAILFMDGRRDRQRLADVTAAKAEVTAVQDNLRAIIEAMPIGTIIVDPDGDQVRF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
INPSAAALVDPSPDHPEWRRLIRAAFEAAIDENGRKTGTLSMAFAKPLGDIVSLRGAVCG
ECCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHEE
VIWEGRPQILILLTDTSRVRNAELQLMQAAKLATLGEMATAIAHELNQPLAVIKMAVANA
EEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RRLVLSEADPEAITSKLDRIGAQVDRAKRITDQVRRYARMPGDQTASFVLRQAVELAAGF
HHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VAEQYRAAGIRMVLDLEVSPELLVSGDQTMFEQVIVNLLINSRDAYDSQRQDTHQPQVVV
HHHHHHHCCEEEEEEECCCCHHEECCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHH
RAVVRGGEVVIEVTDDAGGIRNDILGNIFDPFCTTKSAEKGTGLGLSMARNVVRDMNGTI
HHHHCCCEEEEEECCCCCCHHHHHHHHHHCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCE
AAENVEGGARFTIVLPAARTQAQDVA
EEECCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]