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The map label for this gene is hupL [H]

Identifier: 158422223

GI number: 158422223

Start: 666589

End: 668403

Strand: Direct

Name: hupL [H]

Synonym: AZC_0599

Alternate gene names: 158422223

Gene position: 666589-668403 (Clockwise)

Preceding gene: 158422222

Following gene: 158422224

Centisome position: 12.41

GC content: 64.02

Gene sequence:

>1815_bases
GTGACCATCCAGACCCCGAACGGCTTCAGCCTGGATAATGCCGGGCGCCGTATTGTCGTCGATCCCGTCACCCGCATCGA
AGGCCATATGCGCTGCGAGGTGAATGTCGACGCCAACAACGTCATCCGCAACGCGGTCTCCACCGGCACCATGTGGCGCG
GGCTGGAGGTCATCCTGAAGGGCCGCGATCCCCGCGATGCCTGGGCTTTCGTGGAACGCATCTGCGGCGTGTGCACCGGC
TGCCACGCGCTCACCTCCGTGCGCGCGGTGGAGGATGCCCTCCAGATCAAGATCCCGAACAACGCCTTCCTGATCCGCGA
GATCATGGCCAAGGTGCTGCAGTGGCACGACCATGTGGTCCACTTCTATCATCTGCATGCGCTCGACTGGGTGAACCCGG
TCAATGCGCTGAAGGCCGATCCCAAGGCGACCTCCGCCCTCCAGCAGTCCATCTCCAACCATTCCAAGTCCTCGCCGGGC
TATTTCCGCGATGTGCAGAACCGGCTGAAGACCTTCGTGGAGAGCGGGCAGCTCGGCATCTTCAAGAACGGCTACTGGGA
CAATCCCGCTTACAAGCTGCCGCCCGAGGCGGACCTGCTGGCGGTGACGCACTATCTCGAAGCGCTCGATTTTCAGCGGG
AGATCGTGAAGGTCCACACCATCTTCGGCGGCAAGAACCCGCATCCGAACTACATGGTGGGCGGCGTGCCTTGCGCCATC
AATATGGACGGGGACATGTCGGCGGGCGCTCCGCTCAATATGGAGCGCCTCAACTTCGTGAAGCTGAAGCTTCAGGAGGC
GCTGGAATTCTCGGCCAATGTCTATGTGCCGGACGTGATCGCCATCGCCAGCTTCTATAAGGACTGGCTCTATGGCGGCG
GCCTCTCGGCGCTCAACGTGATGGATTATGGCGACTACGAAGCCATCCAGGGCCAGAAGTCCACGGACCGTCTGCCGGGC
GGCGTCATCCTCGGCGGCAAGTGGGACGAGATCCATCCCATCGATCCGCGCGATCCGGCGCAGGTGCAGGAGTTCGTCAC
CCATTCCTGGTACACCTACGGCAGCGGCAACAATGACAAGGGCCTGCACCCCTGGGATGGCGTGACCGAGCCGAAATATG
AGCTCGGCCCCGCCGCCAAGGGCACCAAGACCAATATCGAGGCCGTGGACGAGGCCGCCAAATACAGCTGGATCAAGGCG
CCGCGCTGGCGCGGCAATGCGGTAGAGGTGGGCCCGCTCGCCCGCTACATCCTCGCCTATTCCAAGGGCGTCGATTACGT
GAAGGGGCAGGTGGACGAAGCGCTCGGGCGCTTCAACACCCTCGCCGGCACGAATTTCACGCCCAAGCAGGCGCTGCCCA
CCACCATCGGCCGCACGCTGGCGCGGGCGCTGGAAGCGCATTATTGCGCGGTGATGATGATGGACGACTTCAACCATCTC
ATCGCCAACATCAAGGCGGGCGATACATCCACGGCGAATGTGGAGAAGTGGGACCCCTCCACCTGGCCGAAGGAGGCCAA
GGGCGTCGGCACGGTGGCGGCGCCGCGCGGAGGCCTCGGTCACTGGATCAAGATCAAGGACGGCCGGATCGAGAATTATC
AGTGCGTGGTGCCCACCACCTGGAACGGCGGCCCTCGGGACCCGAAGGGCAACATCGGTGCGTTCGAGGCTTCGCTCCTC
AACACGCCCATGCAGCGCCCCGACGAGCCGGTGGAGATCCTGCGCACGCTGCACTCCTTCGACCCGTGCCTCGCCTGCTC
CACCCACGTGCTTGCGCCGGGCGGGGAGGAACTCGCCCGCGTCACCGTGCGCTGA

Upstream 100 bases:

>100_bases
ACAAGATCGGCTTTGCCGCCGCAGGCGTCGTGGGCGGCGCCGTGGCCGTGCACGCGGCCGTCAGCGCGCTGAAGCGCGCC
CAGCACAAGGGAGACGCCCA

Downstream 100 bases:

>100_bases
GGGGAGGGCGCCATGAGCGACATTCCCCTCTCCCAACCCAAGGCGGCGGAGGCTCCCAAGGAGCGGCGCCTGCCGACGGT
CTACGTCTATGAGGCGCCGG

Product: hydrogenase large subunit protein

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MTIQTPNGFSLDNAGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
CHALTSVRAVEDALQIKIPNNAFLIREIMAKVLQWHDHVVHFYHLHALDWVNPVNALKADPKATSALQQSISNHSKSSPG
YFRDVQNRLKTFVESGQLGIFKNGYWDNPAYKLPPEADLLAVTHYLEALDFQREIVKVHTIFGGKNPHPNYMVGGVPCAI
NMDGDMSAGAPLNMERLNFVKLKLQEALEFSANVYVPDVIAIASFYKDWLYGGGLSALNVMDYGDYEAIQGQKSTDRLPG
GVILGGKWDEIHPIDPRDPAQVQEFVTHSWYTYGSGNNDKGLHPWDGVTEPKYELGPAAKGTKTNIEAVDEAAKYSWIKA
PRWRGNAVEVGPLARYILAYSKGVDYVKGQVDEALGRFNTLAGTNFTPKQALPTTIGRTLARALEAHYCAVMMMDDFNHL
IANIKAGDTSTANVEKWDPSTWPKEAKGVGTVAAPRGGLGHWIKIKDGRIENYQCVVPTTWNGGPRDPKGNIGAFEASLL
NTPMQRPDEPVEILRTLHSFDPCLACSTHVLAPGGEELARVTVR

Sequences:

>Translated_604_residues
MTIQTPNGFSLDNAGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
CHALTSVRAVEDALQIKIPNNAFLIREIMAKVLQWHDHVVHFYHLHALDWVNPVNALKADPKATSALQQSISNHSKSSPG
YFRDVQNRLKTFVESGQLGIFKNGYWDNPAYKLPPEADLLAVTHYLEALDFQREIVKVHTIFGGKNPHPNYMVGGVPCAI
NMDGDMSAGAPLNMERLNFVKLKLQEALEFSANVYVPDVIAIASFYKDWLYGGGLSALNVMDYGDYEAIQGQKSTDRLPG
GVILGGKWDEIHPIDPRDPAQVQEFVTHSWYTYGSGNNDKGLHPWDGVTEPKYELGPAAKGTKTNIEAVDEAAKYSWIKA
PRWRGNAVEVGPLARYILAYSKGVDYVKGQVDEALGRFNTLAGTNFTPKQALPTTIGRTLARALEAHYCAVMMMDDFNHL
IANIKAGDTSTANVEKWDPSTWPKEAKGVGTVAAPRGGLGHWIKIKDGRIENYQCVVPTTWNGGPRDPKGNIGAFEASLL
NTPMQRPDEPVEILRTLHSFDPCLACSTHVLAPGGEELARVTVR
>Mature_603_residues
TIQTPNGFSLDNAGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTGC
HALTSVRAVEDALQIKIPNNAFLIREIMAKVLQWHDHVVHFYHLHALDWVNPVNALKADPKATSALQQSISNHSKSSPGY
FRDVQNRLKTFVESGQLGIFKNGYWDNPAYKLPPEADLLAVTHYLEALDFQREIVKVHTIFGGKNPHPNYMVGGVPCAIN
MDGDMSAGAPLNMERLNFVKLKLQEALEFSANVYVPDVIAIASFYKDWLYGGGLSALNVMDYGDYEAIQGQKSTDRLPGG
VILGGKWDEIHPIDPRDPAQVQEFVTHSWYTYGSGNNDKGLHPWDGVTEPKYELGPAAKGTKTNIEAVDEAAKYSWIKAP
RWRGNAVEVGPLARYILAYSKGVDYVKGQVDEALGRFNTLAGTNFTPKQALPTTIGRTLARALEAHYCAVMMMDDFNHLI
ANIKAGDTSTANVEKWDPSTWPKEAKGVGTVAAPRGGLGHWIKIKDGRIENYQCVVPTTWNGGPRDPKGNIGAFEASLLN
TPMQRPDEPVEILRTLHSFDPCLACSTHVLAPGGEELARVTVR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=601, Percent_Identity=58.5690515806988, Blast_Score=723, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=593, Percent_Identity=42.6644182124789, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66662; Mature: 66531

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQTPNGFSLDNAGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILK
CEECCCCCCCCCCCCCEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHCHHHHCCHHEEE
GRDPRDAWAFVERICGVCTGCHALTSVRAVEDALQIKIPNNAFLIREIMAKVLQWHDHVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHH
HFYHLHALDWVNPVNALKADPKATSALQQSISNHSKSSPGYFRDVQNRLKTFVESGQLGI
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEE
FKNGYWDNPAYKLPPEADLLAVTHYLEALDFQREIVKVHTIFGGKNPHPNYMVGGVPCAI
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCCCEEECCEEEEE
NMDGDMSAGAPLNMERLNFVKLKLQEALEFSANVYVPDVIAIASFYKDWLYGGGLSALNV
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
MDYGDYEAIQGQKSTDRLPGGVILGGKWDEIHPIDPRDPAQVQEFVTHSWYTYGSGNNDK
ECCCCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCC
GLHPWDGVTEPKYELGPAAKGTKTNIEAVDEAAKYSWIKAPRWRGNAVEVGPLARYILAY
CCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHH
SKGVDYVKGQVDEALGRFNTLAGTNFTPKQALPTTIGRTLARALEAHYCAVMMMDDFNHL
HCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHH
IANIKAGDTSTANVEKWDPSTWPKEAKGVGTVAAPRGGLGHWIKIKDGRIENYQCVVPTT
HHCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEECCCCCCCCEEEEECCCCCCEEEEEEEC
WNGGPRDPKGNIGAFEASLLNTPMQRPDEPVEILRTLHSFDPCLACSTHVLAPGGEELAR
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCCEEECCCCHHHHE
VTVR
EECC
>Mature Secondary Structure 
TIQTPNGFSLDNAGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILK
EECCCCCCCCCCCCCEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHCHHHHCCHHEEE
GRDPRDAWAFVERICGVCTGCHALTSVRAVEDALQIKIPNNAFLIREIMAKVLQWHDHVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHH
HFYHLHALDWVNPVNALKADPKATSALQQSISNHSKSSPGYFRDVQNRLKTFVESGQLGI
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEE
FKNGYWDNPAYKLPPEADLLAVTHYLEALDFQREIVKVHTIFGGKNPHPNYMVGGVPCAI
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCCCEEECCEEEEE
NMDGDMSAGAPLNMERLNFVKLKLQEALEFSANVYVPDVIAIASFYKDWLYGGGLSALNV
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
MDYGDYEAIQGQKSTDRLPGGVILGGKWDEIHPIDPRDPAQVQEFVTHSWYTYGSGNNDK
ECCCCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCC
GLHPWDGVTEPKYELGPAAKGTKTNIEAVDEAAKYSWIKAPRWRGNAVEVGPLARYILAY
CCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHH
SKGVDYVKGQVDEALGRFNTLAGTNFTPKQALPTTIGRTLARALEAHYCAVMMMDDFNHL
HCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHH
IANIKAGDTSTANVEKWDPSTWPKEAKGVGTVAAPRGGLGHWIKIKDGRIENYQCVVPTT
HHCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEECCCCCCCCEEEEECCCCCCEEEEEEEC
WNGGPRDPKGNIGAFEASLLNTPMQRPDEPVEILRTLHSFDPCLACSTHVLAPGGEELAR
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCCEEECCCCHHHHE
VTVR
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9324252 [H]