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The map label for this gene is yafC [H]

Identifier: 158422198

GI number: 158422198

Start: 636520

End: 637461

Strand: Reverse

Name: yafC [H]

Synonym: AZC_0574

Alternate gene names: 158422198

Gene position: 637461-636520 (Counterclockwise)

Preceding gene: 158422202

Following gene: 158422196

Centisome position: 11.87

GC content: 70.28

Gene sequence:

>942_bases
GTGCGTAAATCGATGAACCGGAGGTGCAGCGCAGTGACCGGAGCGGACTTCGAGTGGTCGGACCTGCGCTATCTGCTCAC
CGTGGCGCGCTCCGGATCGCTCACCGCCGCCGCCCGGCAGATGGGCGTGGAGCATTCGACGGTGAGCCGCCGCATCACCG
CGCTCGAGACCGCGCTCGGTGCCAAGCTGTTCGACCGGCGGACCGGGGGACTGCTGCTGACGCCGCAGGGCGAGCGGCTC
ATGGCGGCGGCGGAGGGCATGGAGGCGCTCGCCCTCTCCGCCCAGGGGGAGATCGCGGGGGCGGACCTCGGCGTGTCGGG
GACGGTGCGCATCGGCGCGCCGGACGGCTTCGGCACCGTCTTCCTCGCGCCCCGGCTCGGCGCCTTCTCGCGCCAGCATC
CGGCGCTGGAAATCCAGCTCCTCGCCATGCCCCGCCTCATCAGCCTGTCGAAACGCGAGGCGGATATCGCCATCTCGCTG
AGCCGGCCCAAGGAGGGGCGGCTGCATGCCCGCAAGCTCACAGACTACCGGCTCGGCCTTTATGCGGCGGTGGCGTATCT
CGAAACGCACGGCCCCATCGAGAGCCGGGCAGCGCTCGGGCGGCATCCCTTCATCGGCTACATCGACGATCTCATCTATG
CGCCGGAGCTGGACTATGTGCCCCTCGTGGCGCGCGAGCTGAAGCCACGCATCAAGAGCTCCAACCTCGTCGCGCAGATG
GAGGCCACCCGCGCGGGGGCGGGCGTGTGCGTCCTGCCCTGCTTCATGGGGGATGCCATGCCGGACCTTGTGCCGGTGCT
GCGCGGCGAGGTGTCGCTCACGCGCACCTTCTATCTCGTGACCCACACGGACATGCGCGACCTTGCCCGCGTACGGGCGG
CGGCGGATTTCATCGCCTCCGAAGTGGGGCAGGCTCGGGCGCGCTTTCTCGGCGCGCCCTGA

Upstream 100 bases:

>100_bases
CCCGCATGATGCGATCCTCCCGATGATGCTTGTGTTCTGGTTGGGCGCTACGATGCCCTAGCTTTTTGCGCACATCCATC
GCAATTTCCGCACTCGTATC

Downstream 100 bases:

>100_bases
ACGGCGGGATCAGGCGATGGCGGCGGAGGCTTCCCGCACCTTGCGGGCGGCGGCCTCCACATGGCTGGTGGCGCTGGCGA
TCACGCCCATGTCCTGCTTC

Product: transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRKSMNRRCSAVTGADFEWSDLRYLLTVARSGSLTAAARQMGVEHSTVSRRITALETALGAKLFDRRTGGLLLTPQGERL
MAAAEGMEALALSAQGEIAGADLGVSGTVRIGAPDGFGTVFLAPRLGAFSRQHPALEIQLLAMPRLISLSKREADIAISL
SRPKEGRLHARKLTDYRLGLYAAVAYLETHGPIESRAALGRHPFIGYIDDLIYAPELDYVPLVARELKPRIKSSNLVAQM
EATRAGAGVCVLPCFMGDAMPDLVPVLRGEVSLTRTFYLVTHTDMRDLARVRAAADFIASEVGQARARFLGAP

Sequences:

>Translated_313_residues
MRKSMNRRCSAVTGADFEWSDLRYLLTVARSGSLTAAARQMGVEHSTVSRRITALETALGAKLFDRRTGGLLLTPQGERL
MAAAEGMEALALSAQGEIAGADLGVSGTVRIGAPDGFGTVFLAPRLGAFSRQHPALEIQLLAMPRLISLSKREADIAISL
SRPKEGRLHARKLTDYRLGLYAAVAYLETHGPIESRAALGRHPFIGYIDDLIYAPELDYVPLVARELKPRIKSSNLVAQM
EATRAGAGVCVLPCFMGDAMPDLVPVLRGEVSLTRTFYLVTHTDMRDLARVRAAADFIASEVGQARARFLGAP
>Mature_313_residues
MRKSMNRRCSAVTGADFEWSDLRYLLTVARSGSLTAAARQMGVEHSTVSRRITALETALGAKLFDRRTGGLLLTPQGERL
MAAAEGMEALALSAQGEIAGADLGVSGTVRIGAPDGFGTVFLAPRLGAFSRQHPALEIQLLAMPRLISLSKREADIAISL
SRPKEGRLHARKLTDYRLGLYAAVAYLETHGPIESRAALGRHPFIGYIDDLIYAPELDYVPLVARELKPRIKSSNLVAQM
EATRAGAGVCVLPCFMGDAMPDLVPVLRGEVSLTRTFYLVTHTDMRDLARVRAAADFIASEVGQARARFLGAP

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=27.7966101694915, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789440, Length=183, Percent_Identity=28.9617486338798, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081978, Length=173, Percent_Identity=32.3699421965318, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=24.1496598639456, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790011, Length=201, Percent_Identity=27.363184079602, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693193, Length=194, Percent_Identity=28.3505154639175, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787128, Length=146, Percent_Identity=29.4520547945205, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789639, Length=183, Percent_Identity=27.3224043715847, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33818; Mature: 33818

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSMNRRCSAVTGADFEWSDLRYLLTVARSGSLTAAARQMGVEHSTVSRRITALETALG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
AKLFDRRTGGLLLTPQGERLMAAAEGMEALALSAQGEIAGADLGVSGTVRIGAPDGFGTV
HHHHHCCCCCEEECCCCCCHHHHHCCCCEEEEECCCCEECCCCCCCEEEEEECCCCCCEE
FLAPRLGAFSRQHPALEIQLLAMPRLISLSKREADIAISLSRPKEGRLHARKLTDYRLGL
EECCCCCCCCCCCCCEEEEEEECCHHHHCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
YAAVAYLETHGPIESRAALGRHPFIGYIDDLIYAPELDYVPLVARELKPRIKSSNLVAQM
HHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEE
EATRAGAGVCVLPCFMGDAMPDLVPVLRGEVSLTRTFYLVTHTDMRDLARVRAAADFIAS
HHHCCCCCEEEEEHHHCCCHHHHHHHHCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHH
EVGQARARFLGAP
HHHHHHHHHCCCC
>Mature Secondary Structure
MRKSMNRRCSAVTGADFEWSDLRYLLTVARSGSLTAAARQMGVEHSTVSRRITALETALG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
AKLFDRRTGGLLLTPQGERLMAAAEGMEALALSAQGEIAGADLGVSGTVRIGAPDGFGTV
HHHHHCCCCCEEECCCCCCHHHHHCCCCEEEEECCCCEECCCCCCCEEEEEECCCCCCEE
FLAPRLGAFSRQHPALEIQLLAMPRLISLSKREADIAISLSRPKEGRLHARKLTDYRLGL
EECCCCCCCCCCCCCEEEEEEECCHHHHCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
YAAVAYLETHGPIESRAALGRHPFIGYIDDLIYAPELDYVPLVARELKPRIKSSNLVAQM
HHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEE
EATRAGAGVCVLPCFMGDAMPDLVPVLRGEVSLTRTFYLVTHTDMRDLARVRAAADFIAS
HHHCCCCCEEEEEHHHCCCHHHHHHHHCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHH
EVGQARARFLGAP
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]