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The map label for this gene is mesJ [H]

Identifier: 158422151

GI number: 158422151

Start: 582279

End: 583277

Strand: Direct

Name: mesJ [H]

Synonym: AZC_0527

Alternate gene names: 158422151

Gene position: 582279-583277 (Clockwise)

Preceding gene: 158422150

Following gene: 158422152

Centisome position: 10.84

GC content: 72.27

Gene sequence:

>999_bases
GTGTCGGGCGGCCCCGATTCCACGGCCATGCTCGTGCTCGCCCGCATCTGGCGCTACGGGCTGCTGGACGGCCCGAGCTT
CTTCGTGGCGACGGTGGACCACGGCCTGCGCCCCGAGGCGACGGACGAGGCCCTCGCCGTCGGTGCCCTGTGCGAAGAAC
TCGGCCTGCCCCATGTGATCCTGCCGTGGCGCGGGCCGAAGCCGCGCTCCGGCATTCAGGAGGCGGCCCGCGACGAGCGC
TTCGCGCTGCTGCGCTCCTTCGCCCGGCAGAACGGGCTGGATGGCCTCGCGCTTGCCCATACCCAGGACGATCAGGCCGA
GACCGTGCTGTTCCGCCTCTGCCGCGGCTCCGGCATCGGCGGGCTGGCCGCCATGCGGCCGGTGAGCCGGCGCAACGGCC
TCGCGGTGCTGCGGCCCTTCCTGTCCATCGCCAAGGCGCGGCTTGTTGCCACGCTGGCGGTGTCCGGCACCCCCTTCGTG
CGTGATCCAAGTAACGAGGACACGCGCTATGCCCGCCCGCGCCTGCGGGCGATCTCGCCCCTGCTGGGCGCGGAAGGGCT
GGACGCCGCGCGTCTCGCCGTGCTGGCCCAGCGGGCGGCGCGGGCGGACGATGCGCTGGAGGCCGCGACCGATGCCGCGC
AGAAGCTGCTGGTGCATGGCGGCTGGGACGACGAGGCCGGGACGCGCCTTGATGCGGAGGGCTTTTTTCGCCTGCCGCAG
GAAATCGCGCTCCGCCTGCTGATGAGGGCGCTGACCCTGCGCGCCACCGAGGGGCCCGTCGAACTCGCCAAGGCAGAGCG
GCTGCTGGAAGCCCTTCGCGCGGCGGTGATCCAGCCGGGCCGTCTGCGGCGTACTCTGGCAGGAGCCCTGGTCACCCTGA
AGCAAGGCAGTCTGTCTATCGTCCAAGCACCGGCGCGACAGGCTGGGGCAGCCCACCTCGCCGCAAATTCAGGAACGAGC
GGGGCACTCGTCCTTGGCAAGGAGGGGGGCGACCCCTAA

Upstream 100 bases:

>100_bases
CGTGCAGGCTGCTGAGCAGGACTACGCGCCTCCCATCGAGGAGGAAGAACTCGAGGGGCTGTTCGGGATCTTCCGCGGCC
ACCGGCGCATCCTTCTCGGC

Downstream 100 bases:

>100_bases
ATTACGGGTGGACGCCGCGGGACGTGGAGGGGAGCAGGCTTCGTCTGTCTGTGTAAGCCGCTCCGGAACGGATTGCTGCG
CCCCATGAGGGACCAATGAA

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSGGPDSTAMLVLARIWRYGLLDGPSFFVATVDHGLRPEATDEALAVGALCEELGLPHVILPWRGPKPRSGIQEAARDER
FALLRSFARQNGLDGLALAHTQDDQAETVLFRLCRGSGIGGLAAMRPVSRRNGLAVLRPFLSIAKARLVATLAVSGTPFV
RDPSNEDTRYARPRLRAISPLLGAEGLDAARLAVLAQRAARADDALEAATDAAQKLLVHGGWDDEAGTRLDAEGFFRLPQ
EIALRLLMRALTLRATEGPVELAKAERLLEALRAAVIQPGRLRRTLAGALVTLKQGSLSIVQAPARQAGAAHLAANSGTS
GALVLGKEGGDP

Sequences:

>Translated_332_residues
MSGGPDSTAMLVLARIWRYGLLDGPSFFVATVDHGLRPEATDEALAVGALCEELGLPHVILPWRGPKPRSGIQEAARDER
FALLRSFARQNGLDGLALAHTQDDQAETVLFRLCRGSGIGGLAAMRPVSRRNGLAVLRPFLSIAKARLVATLAVSGTPFV
RDPSNEDTRYARPRLRAISPLLGAEGLDAARLAVLAQRAARADDALEAATDAAQKLLVHGGWDDEAGTRLDAEGFFRLPQ
EIALRLLMRALTLRATEGPVELAKAERLLEALRAAVIQPGRLRRTLAGALVTLKQGSLSIVQAPARQAGAAHLAANSGTS
GALVLGKEGGDP
>Mature_331_residues
SGGPDSTAMLVLARIWRYGLLDGPSFFVATVDHGLRPEATDEALAVGALCEELGLPHVILPWRGPKPRSGIQEAARDERF
ALLRSFARQNGLDGLALAHTQDDQAETVLFRLCRGSGIGGLAAMRPVSRRNGLAVLRPFLSIAKARLVATLAVSGTPFVR
DPSNEDTRYARPRLRAISPLLGAEGLDAARLAVLAQRAARADDALEAATDAAQKLLVHGGWDDEAGTRLDAEGFFRLPQE
IALRLLMRALTLRATEGPVELAKAERLLEALRAAVIQPGRLRRTLAGALVTLKQGSLSIVQAPARQAGAAHLAANSGTSG
ALVLGKEGGDP

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=185, Percent_Identity=34.5945945945946, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35150; Mature: 35019

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGPDSTAMLVLARIWRYGLLDGPSFFVATVDHGLRPEATDEALAVGALCEELGLPHVI
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
LPWRGPKPRSGIQEAARDERFALLRSFARQNGLDGLALAHTQDDQAETVLFRLCRGSGIG
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCC
GLAAMRPVSRRNGLAVLRPFLSIAKARLVATLAVSGTPFVRDPSNEDTRYARPRLRAISP
CHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHH
LLGAEGLDAARLAVLAQRAARADDALEAATDAAQKLLVHGGWDDEAGTRLDAEGFFRLPQ
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHCCCH
EIALRLLMRALTLRATEGPVELAKAERLLEALRAAVIQPGRLRRTLAGALVTLKQGSLSI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCE
VQAPARQAGAAHLAANSGTSGALVLGKEGGDP
EECCHHHCCCCEEECCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
SGGPDSTAMLVLARIWRYGLLDGPSFFVATVDHGLRPEATDEALAVGALCEELGLPHVI
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
LPWRGPKPRSGIQEAARDERFALLRSFARQNGLDGLALAHTQDDQAETVLFRLCRGSGIG
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCC
GLAAMRPVSRRNGLAVLRPFLSIAKARLVATLAVSGTPFVRDPSNEDTRYARPRLRAISP
CHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHH
LLGAEGLDAARLAVLAQRAARADDALEAATDAAQKLLVHGGWDDEAGTRLDAEGFFRLPQ
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHCCCH
EIALRLLMRALTLRATEGPVELAKAERLLEALRAAVIQPGRLRRTLAGALVTLKQGSLSI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCE
VQAPARQAGAAHLAANSGTSGALVLGKEGGDP
EECCHHHCCCCEEECCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA