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The map label for this gene is gltD [H]

Identifier: 158422085

GI number: 158422085

Start: 516489

End: 517964

Strand: Direct

Name: gltD [H]

Synonym: AZC_0461

Alternate gene names: 158422085

Gene position: 516489-517964 (Clockwise)

Preceding gene: 158422084

Following gene: 158422087

Centisome position: 9.62

GC content: 69.24

Gene sequence:

>1476_bases
ATGGGAGCTGCGGCGCGGTGCGCGCGACGAGGCTCCGCGGAGGGGTGTCTGATGGGTAAGGTCACGGGGTTCCTGGAGAT
CGATCGGCACGATCAGAAGTATCAGCCGGCGTCCGATCGCATTCGCCATTTCCGCGAGTTCACCCTGCCGCTGCCGGAGA
CGGAAGTCAGCAACCAGGCGGCGCGCTGCATGGATTGCGGCATCCCGTTCTGCCATGGCGACACCGGCTGCCCGGTGCAC
AACCAGATCCCGGACTGGAACGACCTCGTCTATAATGGCGAGTGGGAGGAAGCGGCCCGCAACCTCCACTCCACGAACAA
TTTCCCCGAGTTCACCGGCCGCGTCTGCCCCGCCCCGTGCGAGGAAGCCTGCACGCTGAACCTCGAGGACATGCCGGTCA
CCATCAAGACCGTGGAGCAGTCCATCGCCGACAAGGCGTGGAAGATGGGCTGGATCAAGCCCGAGCCCCCGGCCCACCGC
ACCGGACGTTCCGTCGCCGTGGTCGGCTCCGGCCCGGCAGGCCTTGCCGCCGCCCAGCAGCTCGCCCGTGCCGGCCATGA
CGTGCACGTCTTCGAGCGCGAGCCCAAGGCCGGCGGCCTGCTGCGCTACGGCATCCCGGACTTCAAGATGGAGAAGCACC
ACATCGACCGCCGCGTGGCGCAGATGGAAGCGGAGGGCGTGACCTTCCACTACGGCGTCAATGTGGGCGTGACGAAGCCG
GTGCAGGAACTGCTCGACAGCTATGACGCGGTGCTGCTCGCCGGCGGCGCCGAGGCCCCGCGCGATCCCAAGCTGCCGGG
CCAGGAGCTGGAAGGCGTGCATTTCGCCATGCCCTATCTGGTGCAGCAGAACCGCCGCGTGGGCCATGAGGACACCGGCC
GCGAGCATCCGCTGCTGGCTTCCGGCCGCCATGTGGTGGTGGTGGGCGGCGGCGACACCGCCTCCGACTGCGTGGGCACC
GCTTTCCGCCAGGGCGCGCTCTCCGTCACCCAGCTCGACATCCGCCCGGTGCCGCCGGTCAAGGAGAACAAGCTGGCGGT
GTGGCCCTTCTGGCCGACCAAGTTCCGCACCTCGTCCTCGCAGGCCGAGGGCGCCGAGCGCGAGTTCGCCTGCGCCACGC
TCGGCATCGAGGGGCGCAACGGCCGCGTGACCGGCGTGAAGTGCGCCCGCGTCGACCACAAGCGCCAGCCGATCCCGGGC
ACGGAATTCGTGCTGCGCGCCGATCTCGTCTTCCTCGCCATCGGCTTCGCGAGCCCCATCCATGAGGGCCTGCTGGAGCA
GTCGGGCGCGGATCTCGACGGGCGCGGCAATGTGAAGGCGAACGTCACGGACTATCAGACCTCCGTGCCGAAGCTCTATG
CGGCGGGCGACATGCGGCGCGGCCAGTCGCTGGTGGTCTGGGCCATCCGCGAGGGCCGTCAGGCGGCCCACGCCATTGAC
CTCGCCCTCATGGGCACCACCACCCTGCCGCGCTGA

Upstream 100 bases:

>100_bases
CCTCCGACCCGAACCTTCATTTGCGCCCCGCGCCGGCGAAAGGGCTGGCGTGCGCAAAAAGTGACGGGTCGAGAGTCCTC
AAGCTGGAACAATTCTTGTA

Downstream 100 bases:

>100_bases
CCTGCGGCACCTTCTGAAACGAAACGGCCGGGCGTTTGCCCGGCCGTTTGCATTTCCGATGGTGCGTTCCGGGCCCGGCG
CGGCGCTTCCTCAGACTAGA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MGAAARCARRGSAEGCLMGKVTGFLEIDRHDQKYQPASDRIRHFREFTLPLPETEVSNQAARCMDCGIPFCHGDTGCPVH
NQIPDWNDLVYNGEWEEAARNLHSTNNFPEFTGRVCPAPCEEACTLNLEDMPVTIKTVEQSIADKAWKMGWIKPEPPAHR
TGRSVAVVGSGPAGLAAAQQLARAGHDVHVFEREPKAGGLLRYGIPDFKMEKHHIDRRVAQMEAEGVTFHYGVNVGVTKP
VQELLDSYDAVLLAGGAEAPRDPKLPGQELEGVHFAMPYLVQQNRRVGHEDTGREHPLLASGRHVVVVGGGDTASDCVGT
AFRQGALSVTQLDIRPVPPVKENKLAVWPFWPTKFRTSSSQAEGAEREFACATLGIEGRNGRVTGVKCARVDHKRQPIPG
TEFVLRADLVFLAIGFASPIHEGLLEQSGADLDGRGNVKANVTDYQTSVPKLYAAGDMRRGQSLVVWAIREGRQAAHAID
LALMGTTTLPR

Sequences:

>Translated_491_residues
MGAAARCARRGSAEGCLMGKVTGFLEIDRHDQKYQPASDRIRHFREFTLPLPETEVSNQAARCMDCGIPFCHGDTGCPVH
NQIPDWNDLVYNGEWEEAARNLHSTNNFPEFTGRVCPAPCEEACTLNLEDMPVTIKTVEQSIADKAWKMGWIKPEPPAHR
TGRSVAVVGSGPAGLAAAQQLARAGHDVHVFEREPKAGGLLRYGIPDFKMEKHHIDRRVAQMEAEGVTFHYGVNVGVTKP
VQELLDSYDAVLLAGGAEAPRDPKLPGQELEGVHFAMPYLVQQNRRVGHEDTGREHPLLASGRHVVVVGGGDTASDCVGT
AFRQGALSVTQLDIRPVPPVKENKLAVWPFWPTKFRTSSSQAEGAEREFACATLGIEGRNGRVTGVKCARVDHKRQPIPG
TEFVLRADLVFLAIGFASPIHEGLLEQSGADLDGRGNVKANVTDYQTSVPKLYAAGDMRRGQSLVVWAIREGRQAAHAID
LALMGTTTLPR
>Mature_490_residues
GAAARCARRGSAEGCLMGKVTGFLEIDRHDQKYQPASDRIRHFREFTLPLPETEVSNQAARCMDCGIPFCHGDTGCPVHN
QIPDWNDLVYNGEWEEAARNLHSTNNFPEFTGRVCPAPCEEACTLNLEDMPVTIKTVEQSIADKAWKMGWIKPEPPAHRT
GRSVAVVGSGPAGLAAAQQLARAGHDVHVFEREPKAGGLLRYGIPDFKMEKHHIDRRVAQMEAEGVTFHYGVNVGVTKPV
QELLDSYDAVLLAGGAEAPRDPKLPGQELEGVHFAMPYLVQQNRRVGHEDTGREHPLLASGRHVVVVGGGDTASDCVGTA
FRQGALSVTQLDIRPVPPVKENKLAVWPFWPTKFRTSSSQAEGAEREFACATLGIEGRNGRVTGVKCARVDHKRQPIPGT
EFVLRADLVFLAIGFASPIHEGLLEQSGADLDGRGNVKANVTDYQTSVPKLYAAGDMRRGQSLVVWAIREGRQAAHAIDL
ALMGTTTLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=483, Percent_Identity=25.0517598343685, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI111118983, Length=154, Percent_Identity=35.0649350649351, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI111118981, Length=154, Percent_Identity=35.0649350649351, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1789606, Length=476, Percent_Identity=38.655462184874, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1788811, Length=453, Percent_Identity=38.1898454746137, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI87082180, Length=456, Percent_Identity=35.9649122807018, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788468, Length=450, Percent_Identity=27.7777777777778, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789243, Length=428, Percent_Identity=29.9065420560748, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17570289, Length=492, Percent_Identity=41.6666666666667, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984108, Length=453, Percent_Identity=25.3863134657837, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=174, Percent_Identity=35.0574712643678, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=493, Percent_Identity=43.0020283975659, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=46.9135802469136, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=46.9135802469136, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=46.9135802469136, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=46.9135802469136, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24640763, Length=457, Percent_Identity=25.601750547046, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18858217, Length=457, Percent_Identity=25.601750547046, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137178, Length=173, Percent_Identity=30.635838150289, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53520; Mature: 53389

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAAARCARRGSAEGCLMGKVTGFLEIDRHDQKYQPASDRIRHFREFTLPLPETEVSNQA
CCHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
ARCMDCGIPFCHGDTGCPVHNQIPDWNDLVYNGEWEEAARNLHSTNNFPEFTGRVCPAPC
HHHHHCCCCEECCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCHHCCCCCCCCC
EEACTLNLEDMPVTIKTVEQSIADKAWKMGWIKPEPPAHRTGRSVAVVGSGPAGLAAAQQ
CHHHEECCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
LARAGHDVHVFEREPKAGGLLRYGIPDFKMEKHHIDRRVAQMEAEGVTFHYGVNVGVTKP
HHHCCCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHH
VQELLDSYDAVLLAGGAEAPRDPKLPGQELEGVHFAMPYLVQQNRRVGHEDTGREHPLLA
HHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCEEEHHHHHHHHCCCCCCCCCCCCCCEEE
SGRHVVVVGGGDTASDCVGTAFRQGALSVTQLDIRPVPPVKENKLAVWPFWPTKFRTSSS
CCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCC
QAEGAEREFACATLGIEGRNGRVTGVKCARVDHKRQPIPGTEFVLRADLVFLAIGFASPI
CCCCCCCEEEEEEEEEECCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HEGLLEQSGADLDGRGNVKANVTDYQTSVPKLYAAGDMRRGQSLVVWAIREGRQAAHAID
HHHHHHHCCCCCCCCCCEEEECCHHHCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHEE
LALMGTTTLPR
EEEECCCCCCC
>Mature Secondary Structure 
GAAARCARRGSAEGCLMGKVTGFLEIDRHDQKYQPASDRIRHFREFTLPLPETEVSNQA
CHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
ARCMDCGIPFCHGDTGCPVHNQIPDWNDLVYNGEWEEAARNLHSTNNFPEFTGRVCPAPC
HHHHHCCCCEECCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCHHCCCCCCCCC
EEACTLNLEDMPVTIKTVEQSIADKAWKMGWIKPEPPAHRTGRSVAVVGSGPAGLAAAQQ
CHHHEECCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
LARAGHDVHVFEREPKAGGLLRYGIPDFKMEKHHIDRRVAQMEAEGVTFHYGVNVGVTKP
HHHCCCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHH
VQELLDSYDAVLLAGGAEAPRDPKLPGQELEGVHFAMPYLVQQNRRVGHEDTGREHPLLA
HHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCEEEHHHHHHHHCCCCCCCCCCCCCCEEE
SGRHVVVVGGGDTASDCVGTAFRQGALSVTQLDIRPVPPVKENKLAVWPFWPTKFRTSSS
CCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCC
QAEGAEREFACATLGIEGRNGRVTGVKCARVDHKRQPIPGTEFVLRADLVFLAIGFASPI
CCCCCCCEEEEEEEEEECCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HEGLLEQSGADLDGRGNVKANVTDYQTSVPKLYAAGDMRRGQSLVVWAIREGRQAAHAID
HHHHHHHCCCCCCCCCCEEEECCHHHCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHEE
LALMGTTTLPR
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]