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The map label for this gene is ccrM [H]

Identifier: 158422077

GI number: 158422077

Start: 505815

End: 507002

Strand: Direct

Name: ccrM [H]

Synonym: AZC_0453

Alternate gene names: 158422077

Gene position: 505815-507002 (Clockwise)

Preceding gene: 158422076

Following gene: 158422081

Centisome position: 9.42

GC content: 68.6

Gene sequence:

>1188_bases
TTGCGTCTCATGAGTGTGGTGCGTAAAGGGGCGCCCCGCCGGGCGCCCCGCCTCTCCGGCGGGGTCGACTTCGCGGAACC
GTCTCGCGCGATCCCGACGGACCGCATTCTGATCGGCGATTGCGTCGAGCAACTGGCGAGCCTGCCGCCGCACAGCGTCG
ACATGGTCTTCGCCGATCCGCCCTACAATCTCCAGCTCCAGGGCGAACTGAAGCGTCCCGACGACAGCCGCGTCGATGCG
GTTGACGATGCCTGGGACCAGTTCGAGAGCTTCGAGGCCTACGATGCCTTCACCCGCGCCTGGCTGCTGGCGGTGAAGCG
GGTCATGAAGCCGAACGCGACCATCTGGGTCATCGGCTCCTATCACAACATCTTCCGCGTCGGCGCGCTGATGCAGGATC
TGGGCTTCTGGATCCTGAACGACGTGGTGTGGCGCAAGTCGAACCCGATGCCGAACTTCCGCGGCCGGCGGTTCACCAAC
GCCCACGAGACCATGATCTGGGCCGCGCGCGATCCCGGCGCCAAGGGCTACCAGTTCAATTACGAGGCGCTGAAGGGCGG
CAATGAGGATGTGCAGGTGCGCTCCGACTGGCTGCTACCCATCTGCACCGGCGGCGAGCGCCTCAAGGATGCGCAGGGCC
GCAAGCTGCACCCGACGCAGAAGCCGGAAGCGCTGCTCGCCCGCGTGATGCTCTCGGCCTCCAAGCCCGGCGACGTGGTG
CTCGATCCCTTCCTCGGCTCCGGCACCAGCGCGGCGGTGGCCAAGCGCCTGCGTCGCCACTTCGTCGGCGTCGAGCGCGA
CGAGACCTATGCGGCGGCCGCCGCCGCCCGCATCGATGCCATCGAGCCGCTGCCGGAAGCCGCCCTTGTGGCCCCGCCCA
GCGCCCGCGAGGCGCCCCGCGTCGCCTTCTCGGCGCTGGTGGACAGTGGGCTTGTGACGCCCGGCCTCGAACTCACCGAC
GCCAAGGGACATGTCCGGGCCGTGGTGCGCGCCGATGGCACCATCGCGCTCGTGAATGCCGCCACGCTGGCGGTGGGCTC
CATTCATCGCATGGGCGCGCTGGCGCAAGGCGCCGAAGCCTGCAACGGCTGGACATTCTGGCATGTGGAGCAGGAAGGCC
GCCGCCATCCCATCGATGTGCTGCGTGCCCGCCTGCGAGCCGAAATGGGCATCCGCTCGGAAGAGTGA

Upstream 100 bases:

>100_bases
GCCGCGTAAACGCGCAAATATTTGCGAAATGCCCTGACGCTAACTTCGCCTTTACCGGCGGCGCGCAACACTCTGCCCCA
CAGTAAGCGTTGCGTATGGG

Downstream 100 bases:

>100_bases
GCCGAAACGATTTTCTCAAAACGGCAATGGGGCGGGCGGATCATCTCCGCTCGCCCCATTTGCGTTGAGGGCTTCAGGTC
AGCCGTTCGGCGGTCTTTGC

Product: adenine DNA methyltransferase

Products: NA

Alternate protein names: M.BabI; Adenine-specific methyltransferase BabI; DNA methyltransferase CcrM; M.CcrMI ortholog [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRLMSVVRKGAPRRAPRLSGGVDFAEPSRAIPTDRILIGDCVEQLASLPPHSVDMVFADPPYNLQLQGELKRPDDSRVDA
VDDAWDQFESFEAYDAFTRAWLLAVKRVMKPNATIWVIGSYHNIFRVGALMQDLGFWILNDVVWRKSNPMPNFRGRRFTN
AHETMIWAARDPGAKGYQFNYEALKGGNEDVQVRSDWLLPICTGGERLKDAQGRKLHPTQKPEALLARVMLSASKPGDVV
LDPFLGSGTSAAVAKRLRRHFVGVERDETYAAAAAARIDAIEPLPEAALVAPPSAREAPRVAFSALVDSGLVTPGLELTD
AKGHVRAVVRADGTIALVNAATLAVGSIHRMGALAQGAEACNGWTFWHVEQEGRRHPIDVLRARLRAEMGIRSEE

Sequences:

>Translated_395_residues
MRLMSVVRKGAPRRAPRLSGGVDFAEPSRAIPTDRILIGDCVEQLASLPPHSVDMVFADPPYNLQLQGELKRPDDSRVDA
VDDAWDQFESFEAYDAFTRAWLLAVKRVMKPNATIWVIGSYHNIFRVGALMQDLGFWILNDVVWRKSNPMPNFRGRRFTN
AHETMIWAARDPGAKGYQFNYEALKGGNEDVQVRSDWLLPICTGGERLKDAQGRKLHPTQKPEALLARVMLSASKPGDVV
LDPFLGSGTSAAVAKRLRRHFVGVERDETYAAAAAARIDAIEPLPEAALVAPPSAREAPRVAFSALVDSGLVTPGLELTD
AKGHVRAVVRADGTIALVNAATLAVGSIHRMGALAQGAEACNGWTFWHVEQEGRRHPIDVLRARLRAEMGIRSEE
>Mature_395_residues
MRLMSVVRKGAPRRAPRLSGGVDFAEPSRAIPTDRILIGDCVEQLASLPPHSVDMVFADPPYNLQLQGELKRPDDSRVDA
VDDAWDQFESFEAYDAFTRAWLLAVKRVMKPNATIWVIGSYHNIFRVGALMQDLGFWILNDVVWRKSNPMPNFRGRRFTN
AHETMIWAARDPGAKGYQFNYEALKGGNEDVQVRSDWLLPICTGGERLKDAQGRKLHPTQKPEALLARVMLSASKPGDVV
LDPFLGSGTSAAVAKRLRRHFVGVERDETYAAAAAARIDAIEPLPEAALVAPPSAREAPRVAFSALVDSGLVTPGLELTD
AKGHVRAVVRADGTIALVNAATLAVGSIHRMGALAQGAEACNGWTFWHVEQEGRRHPIDVLRARLRAEMGIRSEE

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=280, Percent_Identity=30.3571428571429, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 43438; Mature: 43438

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLMSVVRKGAPRRAPRLSGGVDFAEPSRAIPTDRILIGDCVEQLASLPPHSVDMVFADP
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
PYNLQLQGELKRPDDSRVDAVDDAWDQFESFEAYDAFTRAWLLAVKRVMKPNATIWVIGS
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
YHNIFRVGALMQDLGFWILNDVVWRKSNPMPNFRGRRFTNAHETMIWAARDPGAKGYQFN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCEEEC
YEALKGGNEDVQVRSDWLLPICTGGERLKDAQGRKLHPTQKPEALLARVMLSASKPGDVV
HHHHCCCCCCEEEECCEEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
LDPFLGSGTSAAVAKRLRRHFVGVERDETYAAAAAARIDAIEPLPEAALVAPPSAREAPR
ECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHH
VAFSALVDSGLVTPGLELTDAKGHVRAVVRADGTIALVNAATLAVGSIHRMGALAQGAEA
HHHHHHHHCCCCCCCCEEECCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
CNGWTFWHVEQEGRRHPIDVLRARLRAEMGIRSEE
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLMSVVRKGAPRRAPRLSGGVDFAEPSRAIPTDRILIGDCVEQLASLPPHSVDMVFADP
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
PYNLQLQGELKRPDDSRVDAVDDAWDQFESFEAYDAFTRAWLLAVKRVMKPNATIWVIGS
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
YHNIFRVGALMQDLGFWILNDVVWRKSNPMPNFRGRRFTNAHETMIWAARDPGAKGYQFN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCEEEC
YEALKGGNEDVQVRSDWLLPICTGGERLKDAQGRKLHPTQKPEALLARVMLSASKPGDVV
HHHHCCCCCCEEEECCEEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
LDPFLGSGTSAAVAKRLRRHFVGVERDETYAAAAAARIDAIEPLPEAALVAPPSAREAPR
ECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHH
VAFSALVDSGLVTPGLELTDAKGHVRAVVRADGTIALVNAATLAVGSIHRMGALAQGAEA
HHHHHHHHCCCCCCCCEEECCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
CNGWTFWHVEQEGRRHPIDVLRARLRAEMGIRSEE
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]