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The map label for this gene is lysR [H]

Identifier: 158421915

GI number: 158421915

Start: 339413

End: 340300

Strand: Direct

Name: lysR [H]

Synonym: AZC_0291

Alternate gene names: 158421915

Gene position: 339413-340300 (Clockwise)

Preceding gene: 158421913

Following gene: 158421917

Centisome position: 6.32

GC content: 71.4

Gene sequence:

>888_bases
GTGCGCTTTGACAGCGTCGATCTCAAGCTTTTCCTCGCCATATTGGAAACCGGCAGCATCACGGCCGGAGCCGAGCGGGC
GAACCTCGCCCTGCCCTCGGCGAGCGCGCGGGTGCGCGGCATGGAGGAGGTGGCGGGCACGCCGCTCCTCGTGCGCGGGC
GCAGTGGCGCGACGCCGACGCCGGCGGGGGAGGCACTGGCCCATCACGCCCGCCTCATCCTCCAGCAATCCGAGCGGATG
CGCGGCGAGCTGTCCCGCTACGGGCGCGGGCTGAAGGGGCATGTGCGGCTCTGGACCAGCACGGCGGCCCTCTCGGCCCA
GCTTCCCGTCGATCTCGCCCGCTTCCTTGCGCGGCATCCCGACGTGGACATCGATCTTGAGGAGCGTCCGAGCCGGGAGA
CGGCGCCGGCCTTGCGGGCCGGGCTCATCCACATCGGGCTCCTCGCCGATGCGGCCGATGTCACCGGCTTCGAGACACGG
CCCTATTGCGAGGATGTGCTGATGGCCGTGCTGCCCCTTGCCCACCCGCTGGCCGGCCGCGCGGGGCTGACCTTCGCCGA
GGTCGCAGCCCAGCCCTATGTGGGGCTCACCCCCGGCAATGCCATCCAGCATCTCATCGAGCACGCGGCCATCGGCCTCG
GCCTGCCGCTGAAATACCGGGTGCGGGTGAACCGTATCGATGCGGTGGGCGAATTGGTGCAGGCGGGCGTCGGCGTCTCC
GTCCTGCCAACCGCCGATGCCCGGCGGGTGGCGGAGCGGATGGCGGTGTCGCTGTTTCCGCTGCCCGATGCCTGGGCGCG
CCGCAAGGTGGTGGTGGCCGTGCGCAATCGCGAGGCGCTGCCGGAATTTGCCCGTCAGGCCTTCGACATGCTCGCGGGCG
CGGGCTGA

Upstream 100 bases:

>100_bases
GGTCAGTGCGGTCATGCGCCGCAGGCTGGCCTACGCCCCATCTTGCGGCAATGTGGATTATCCGGCCATATCCTTCGCAT
AATCCGAAGGATCAGATGCC

Downstream 100 bases:

>100_bases
TTTCAGCGCAGCGCGTTCCCCAGCACGCGCAGCACCTCGCCATCCATCTGCGCCACGTTGAAGCGCATCAGGTCGCCAGC
CGACTGGGTGACGCTGAAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRFDSVDLKLFLAILETGSITAGAERANLALPSASARVRGMEEVAGTPLLVRGRSGATPTPAGEALAHHARLILQQSERM
RGELSRYGRGLKGHVRLWTSTAALSAQLPVDLARFLARHPDVDIDLEERPSRETAPALRAGLIHIGLLADAADVTGFETR
PYCEDVLMAVLPLAHPLAGRAGLTFAEVAAQPYVGLTPGNAIQHLIEHAAIGLGLPLKYRVRVNRIDAVGELVQAGVGVS
VLPTADARRVAERMAVSLFPLPDAWARRKVVVAVRNREALPEFARQAFDMLAGAG

Sequences:

>Translated_295_residues
MRFDSVDLKLFLAILETGSITAGAERANLALPSASARVRGMEEVAGTPLLVRGRSGATPTPAGEALAHHARLILQQSERM
RGELSRYGRGLKGHVRLWTSTAALSAQLPVDLARFLARHPDVDIDLEERPSRETAPALRAGLIHIGLLADAADVTGFETR
PYCEDVLMAVLPLAHPLAGRAGLTFAEVAAQPYVGLTPGNAIQHLIEHAAIGLGLPLKYRVRVNRIDAVGELVQAGVGVS
VLPTADARRVAERMAVSLFPLPDAWARRKVVVAVRNREALPEFARQAFDMLAGAG
>Mature_295_residues
MRFDSVDLKLFLAILETGSITAGAERANLALPSASARVRGMEEVAGTPLLVRGRSGATPTPAGEALAHHARLILQQSERM
RGELSRYGRGLKGHVRLWTSTAALSAQLPVDLARFLARHPDVDIDLEERPSRETAPALRAGLIHIGLLADAADVTGFETR
PYCEDVLMAVLPLAHPLAGRAGLTFAEVAAQPYVGLTPGNAIQHLIEHAAIGLGLPLKYRVRVNRIDAVGELVQAGVGVS
VLPTADARRVAERMAVSLFPLPDAWARRKVVVAVRNREALPEFARQAFDMLAGAG

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=260, Percent_Identity=29.6153846153846, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788887, Length=194, Percent_Identity=28.8659793814433, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI157672245, Length=256, Percent_Identity=25.78125, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=24.5136186770428, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788297, Length=256, Percent_Identity=27.734375, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=20.9876543209877, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=255, Percent_Identity=24.7058823529412, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786713, Length=282, Percent_Identity=26.241134751773, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1788481, Length=246, Percent_Identity=23.5772357723577, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31549; Mature: 31549

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDSVDLKLFLAILETGSITAGAERANLALPSASARVRGMEEVAGTPLLVRGRSGATPT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHCCHHHHCCCCEEEECCCCCCCC
PAGEALAHHARLILQQSERMRGELSRYGRGLKGHVRLWTSTAALSAQLPVDLARFLARHP
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHCC
DVDIDLEERPSRETAPALRAGLIHIGLLADAADVTGFETRPYCEDVLMAVLPLAHPLAGR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCC
AGLTFAEVAAQPYVGLTPGNAIQHLIEHAAIGLGLPLKYRVRVNRIDAVGELVQAGVGVS
CCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCEE
VLPTADARRVAERMAVSLFPLPDAWARRKVVVAVRNREALPEFARQAFDMLAGAG
EECCCHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRFDSVDLKLFLAILETGSITAGAERANLALPSASARVRGMEEVAGTPLLVRGRSGATPT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHCCHHHHCCCCEEEECCCCCCCC
PAGEALAHHARLILQQSERMRGELSRYGRGLKGHVRLWTSTAALSAQLPVDLARFLARHP
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHCC
DVDIDLEERPSRETAPALRAGLIHIGLLADAADVTGFETRPYCEDVLMAVLPLAHPLAGR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCC
AGLTFAEVAAQPYVGLTPGNAIQHLIEHAAIGLGLPLKYRVRVNRIDAVGELVQAGVGVS
CCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCEE
VLPTADARRVAERMAVSLFPLPDAWARRKVVVAVRNREALPEFARQAFDMLAGAG
EECCCHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]