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The map label for this gene is fumC

Identifier: 15836544

GI number: 15836544

Start: 1158651

End: 1160033

Strand: Direct

Name: fumC

Synonym: CPj1013

Alternate gene names: 15836544

Gene position: 1158651-1160033 (Clockwise)

Preceding gene: 15836539

Following gene: 15836546

Centisome position: 94.46

GC content: 40.27

Gene sequence:

>1383_bases
ATGCGACAAGAAAAGGATAGTTTAGGAATCGTAGAAGTTCCTGAGGATAAGTTATATGGAGCTCAAACTATGCGTTCTAG
GAATTTTTTTTCTTGGGGACCTGAGTTGATGCCTTATGAGGTAATACGAGCTCTCGTATGGATTAAAAAATGTGCTGCTC
AGGCGAATCAAGATTTAGGATTTTTGGATTCCAAGCATTGCGATATGATTGTTGCTGCTGCCGATGAGATTTTAGAGGGA
GGTTTTGAAGAGCATTTCCCTTTAAAAGTTTGGCAGACAGGGAGCGGCACACAATCTAATATGAATGTGAATGAGGTGAT
TGCGAATCTTGCCATTCGTCATCACGGAGGGGTGTTAGGCAGTAAGGATCCTATCCATCCTAATGATCATGTGAATAAGT
CCCAATCGTCCAATGATGTTTTCCCTACAGCAATGCATATCGCTGCTGTGATTAGTTTAAAAAATAAGTTAATTCCAGCT
TTAGATCATATGATTCGGGTGTTAGATGCTAAAGTGGAAGAATTTCGTCATGATGTAAAGATAGGACGGACCCATCTTAT
GGATGCGGTGCCTATGACGTTGGGTCAGGAATTTTCTGGTTATAGCAGTCAATTGCGTCACTGCTTAGAGAGTATAGCAT
TTTCTTTAGCTCATTTATATGAACTTGCGATTGGAGCTACTGCTGTAGGGACTGGGTTGAATGTTCCTGAAGGGTTCGTG
GAAAAGATCATCCATTATTTAAGGAAGGAAACAGATGAACCGTTTATTCCAGCTTCCAATTATTTTTCAGCACTGTCTTG
TCACGATGCTTTAGTAGATGCCCATGGGTCTTTAGCAACTTTAGCATGTGCTTTAACTAAGATAGCTACGGATTTGAGCT
TTTTAGGTTCAGGACCCAGGTGTGGGTTGGGTGAGTTATTTTTCCCTGAAAATGAACCAGGATCTTCTATCATGCCTGGT
AAAGTCAATCCTACGCAGTGTGAAGCTCTCCAAATGGTTTGTGCTCAAGTTCTTGGGAATAATCAAACAGTGATTATTGG
AGGAAGTCGAGGAAATTTTGAGCTTAATGTGATGAAGCCTGTGATCATCTATAATTTCCTGCAGTCTGTGGATCTCCTTT
CTGAGGGGATGAGGGCTTTCTCTGAATTCTTTGTGAAAGGATTAAAAGTAAATAAAGCTCGTTTACAAGATAATATCAAT
AATTCTTTGATGTTGGTTACAGCTTTAGCTCCTGTATTAGGTTACGACAAGTGTTCGAAAGCAGCACTGAAAGCATTTCA
TGAATCTATATCTTTGAAGGAGGCGTGTCTAGCTTTGGGATATCTTTCTGAGAAGGAATTTGATCGTTTAGTGGTTCCTG
AGAATATGGTGGGAAACCATTAG

Upstream 100 bases:

>100_bases
ATAGCAGGGAAAAAACGATAACAAAGTAGAAAATAAATTTTTTTTTCATTTTTGAAGTAATTAACGAGAAAATAGCCTAA
ATCATAGGGGAAATATAGAT

Downstream 100 bases:

>100_bases
AATTTTTTAAAGAGAGCGAACGCTGAGTGTATTGTAAAGTACCGTAGCGTACGTTTTCTCTTCCCTTGTTATGAAAATCT
TTAAGGTTGCTTTTCTAGGA

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MRQEKDSLGIVEVPEDKLYGAQTMRSRNFFSWGPELMPYEVIRALVWIKKCAAQANQDLGFLDSKHCDMIVAAADEILEG
GFEEHFPLKVWQTGSGTQSNMNVNEVIANLAIRHHGGVLGSKDPIHPNDHVNKSQSSNDVFPTAMHIAAVISLKNKLIPA
LDHMIRVLDAKVEEFRHDVKIGRTHLMDAVPMTLGQEFSGYSSQLRHCLESIAFSLAHLYELAIGATAVGTGLNVPEGFV
EKIIHYLRKETDEPFIPASNYFSALSCHDALVDAHGSLATLACALTKIATDLSFLGSGPRCGLGELFFPENEPGSSIMPG
KVNPTQCEALQMVCAQVLGNNQTVIIGGSRGNFELNVMKPVIIYNFLQSVDLLSEGMRAFSEFFVKGLKVNKARLQDNIN
NSLMLVTALAPVLGYDKCSKAALKAFHESISLKEACLALGYLSEKEFDRLVVPENMVGNH

Sequences:

>Translated_460_residues
MRQEKDSLGIVEVPEDKLYGAQTMRSRNFFSWGPELMPYEVIRALVWIKKCAAQANQDLGFLDSKHCDMIVAAADEILEG
GFEEHFPLKVWQTGSGTQSNMNVNEVIANLAIRHHGGVLGSKDPIHPNDHVNKSQSSNDVFPTAMHIAAVISLKNKLIPA
LDHMIRVLDAKVEEFRHDVKIGRTHLMDAVPMTLGQEFSGYSSQLRHCLESIAFSLAHLYELAIGATAVGTGLNVPEGFV
EKIIHYLRKETDEPFIPASNYFSALSCHDALVDAHGSLATLACALTKIATDLSFLGSGPRCGLGELFFPENEPGSSIMPG
KVNPTQCEALQMVCAQVLGNNQTVIIGGSRGNFELNVMKPVIIYNFLQSVDLLSEGMRAFSEFFVKGLKVNKARLQDNIN
NSLMLVTALAPVLGYDKCSKAALKAFHESISLKEACLALGYLSEKEFDRLVVPENMVGNH
>Mature_460_residues
MRQEKDSLGIVEVPEDKLYGAQTMRSRNFFSWGPELMPYEVIRALVWIKKCAAQANQDLGFLDSKHCDMIVAAADEILEG
GFEEHFPLKVWQTGSGTQSNMNVNEVIANLAIRHHGGVLGSKDPIHPNDHVNKSQSSNDVFPTAMHIAAVISLKNKLIPA
LDHMIRVLDAKVEEFRHDVKIGRTHLMDAVPMTLGQEFSGYSSQLRHCLESIAFSLAHLYELAIGATAVGTGLNVPEGFV
EKIIHYLRKETDEPFIPASNYFSALSCHDALVDAHGSLATLACALTKIATDLSFLGSGPRCGLGELFFPENEPGSSIMPG
KVNPTQCEALQMVCAQVLGNNQTVIIGGSRGNFELNVMKPVIIYNFLQSVDLLSEGMRAFSEFFVKGLKVNKARLQDNIN
NSLMLVTALAPVLGYDKCSKAALKAFHESISLKEACLALGYLSEKEFDRLVVPENMVGNH

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily

Homologues:

Organism=Homo sapiens, GI19743875, Length=459, Percent_Identity=56.2091503267974, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI266458395, Length=63, Percent_Identity=52.3809523809524, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=55.3376906318083, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=39.2624728850325, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=55.4347826086957, Blast_Score=506, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI32565146, Length=325, Percent_Identity=53.5384615384615, Blast_Score=353, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=55, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24640177, Length=460, Percent_Identity=56.5217391304348, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24640179, Length=460, Percent_Identity=56.5217391304348, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=55.3145336225597, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24662684, Length=461, Percent_Identity=54.880694143167, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24583245, Length=461, Percent_Identity=46.2039045553145, Blast_Score=420, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FUMC_CHLPN (Q9Z6P6)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A72009
- PIR:   B86617
- RefSeq:   NP_225207.1
- RefSeq:   NP_301068.1
- RefSeq:   NP_445379.1
- RefSeq:   NP_877323.1
- ProteinModelPortal:   Q9Z6P6
- SMR:   Q9Z6P6
- PHCI-2DPAGE:   Q9Z6P6
- GeneID:   1467730
- GeneID:   894645
- GeneID:   919783
- GeneID:   963045
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0840
- KEGG:   cpn:CPn1013
- KEGG:   cpt:CpB1051
- TIGR:   CP_0840
- HOGENOM:   HBG284369
- OMA:   RIEKDTM
- PhylomeDB:   Q9Z6P6
- ProtClustDB:   PRK00485
- BioCyc:   CPNE115711:CP_0840-MONOMER
- BioCyc:   CPNE115713:CPN1013-MONOMER
- BioCyc:   CPNE138677:CPJ1013-MONOMER
- BioCyc:   CPNE182082:CPB1051-MONOMER
- BRENDA:   4.2.1.2
- HAMAP:   MF_00743
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00979

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.2.1.2

Molecular weight: Translated: 50442; Mature: 50442

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCHHCCCCEECCCHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCHHCCCCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362