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The map label for this gene is lysS

Identifier: 15836464

GI number: 15836464

Start: 1063487

End: 1065070

Strand: Direct

Name: lysS

Synonym: CPj0931

Alternate gene names: 15836464

Gene position: 1063487-1065070 (Clockwise)

Preceding gene: 15836463

Following gene: 15836466

Centisome position: 86.7

GC content: 41.1

Gene sequence:

>1584_bases
ATGACGGCGAGAGCAGAATATTTAGACCATGAGGATTTTTTATATAGGAGTCACAAACTTCAGGAACTTTCTGAGTTGGG
TGTAGTCCTTTATCCTTACGAGTTTCCTGGAGTTTTTTCATGCGAAGATATTAAAAAAACGTTTGCTAGTCAAGAGTTAG
GGAATAGTGAAGCTGCTATGAGTAGGTCCACTCCTAGAGTGCGTTTTGCTGGGCGTTTGGTTCTTTTCCGTGCGATGGGG
AAAAATGCTTTTGGGCAAATTTTAGACCATAATCAAACCATCCAAGTGATGTTCAACCGTGAGTTTACCTCCGTACACGG
ACTTTCTGAAGATGCTGAGATTACTCCGATTAAGTTTATAGAAAAAAAGTTAGATTTAGGAGATATCCTTGGGATCGATG
GCTATTTATTTTTCACCCATTCCGGAGAGCTTACCGTTCTTGTAGAGACTGTAACCCTACTTTGTAAATCCTTACTTTCG
TTGCCTGATAAGCACGCAGGTTTGAGTGACAAAGAAGTCCGCTACCGCAAGCGTTGGTTAGATCTAATTTCCTCAAGAGA
GGTTAGCGATACCTTTGTGAAAAGAAGCTATATTATTAAGCTTATCCGTAATTATATGGATGCTCATGGATTTTTAGAGG
TAGAGACTCCTATATTACAAAATATCTACGGGGGAGCAGAAGCTAAGCCTTTCACAACAACAATGGAAGCTTTACATTCG
GAGACGTTTTTAAGAATCTCCCTGGAAATCGCTTTGAAGAAAATCCTAGTAGGAGGAGCTCCTCGTATTTATGAACTCGG
TAAAGTCTTCAGAAACGAAGGAATCGATAGAACACATAATCCTGAATTTACCATGATAGAAGCGTATGCTGCTTACATGG
ACTATAAAGAGGTCATGGTCTTTGTAGAAAATCTTGTTGAGCATCTTGTCCGTGCTGTAAATCACGACAATACCTCTCTA
GTCTATTCTTATTGGAAACATGGCCCCCAAGAGGTAGATTTTAAAGCTCCTTGGATTCGTATGACAATGAAAGAAAGTAT
CGCGACCTATGCAGGTATTGATGTTGACGTTCACAGTGATCAGAAGCTTAAGGAAATCTTAAAGAAGAAAACTACCTTTC
CTGAGACTGCATTTGCGACAGCTTCCCGGGGGATGTTGATAGCAGCCTTATTTGATGAACTTGTCTCTGATAATTTAATA
GCTCCTCACCACATTACAGATCACCCTGTAGAGACAACCCCGTTATGTAAAACTCTGCGTTCCGGAGATACCGCATTTGT
AGAGCGCTTCGAAAGTTTTTGCTTAGGGAAAGAGTTGTGTAATGCCTATTCGGAACTCAATGATCCTATCCGTCAAAGAG
AACTCTTGGAGCAACAGCATACTAAAAAAGAACTCCTTCCGGATAGTGAGTGTCATCCTATCGATGAAGAGTTTTTAGAA
GCTCTGTGTCAGGGAATGCCCCCTGCAGGAGGATTTGGTATAGGTGTCGATAGACTTGTTATGATTCTTACAAATGCTGC
ATCTATACGCGATGTGCTGTATTTCCCTGTAATGCGGCGCTTCGATGCAGAGAAGACGAATTAA

Upstream 100 bases:

>100_bases
TGTCTGGTGGGATCGGCCTGCTTTTTACAACTTTAGATAGATAGATTTTAGAGTCTTGGGTTGGAAAAGCGATATCTATA
CAAACATACTAGAGGAAAGA

Downstream 100 bases:

>100_bases
AGGGGCTTAACTTTAGGCCCTGACTTAGAGTCTTCGACAAGGAAGCCTGCGGCAAGTATCTCATCACGTAAGGTGTCTGC
CATCGCCCAATTTTTAGTTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTARAEYLDHEDFLYRSHKLQELSELGVVLYPYEFPGVFSCEDIKKTFASQELGNSEAAMSRSTPRVRFAGRLVLFRAMG
KNAFGQILDHNQTIQVMFNREFTSVHGLSEDAEITPIKFIEKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLLS
LPDKHAGLSDKEVRYRKRWLDLISSREVSDTFVKRSYIIKLIRNYMDAHGFLEVETPILQNIYGGAEAKPFTTTMEALHS
ETFLRISLEIALKKILVGGAPRIYELGKVFRNEGIDRTHNPEFTMIEAYAAYMDYKEVMVFVENLVEHLVRAVNHDNTSL
VYSYWKHGPQEVDFKAPWIRMTMKESIATYAGIDVDVHSDQKLKEILKKKTTFPETAFATASRGMLIAALFDELVSDNLI
APHHITDHPVETTPLCKTLRSGDTAFVERFESFCLGKELCNAYSELNDPIRQRELLEQQHTKKELLPDSECHPIDEEFLE
ALCQGMPPAGGFGIGVDRLVMILTNAASIRDVLYFPVMRRFDAEKTN

Sequences:

>Translated_527_residues
MTARAEYLDHEDFLYRSHKLQELSELGVVLYPYEFPGVFSCEDIKKTFASQELGNSEAAMSRSTPRVRFAGRLVLFRAMG
KNAFGQILDHNQTIQVMFNREFTSVHGLSEDAEITPIKFIEKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLLS
LPDKHAGLSDKEVRYRKRWLDLISSREVSDTFVKRSYIIKLIRNYMDAHGFLEVETPILQNIYGGAEAKPFTTTMEALHS
ETFLRISLEIALKKILVGGAPRIYELGKVFRNEGIDRTHNPEFTMIEAYAAYMDYKEVMVFVENLVEHLVRAVNHDNTSL
VYSYWKHGPQEVDFKAPWIRMTMKESIATYAGIDVDVHSDQKLKEILKKKTTFPETAFATASRGMLIAALFDELVSDNLI
APHHITDHPVETTPLCKTLRSGDTAFVERFESFCLGKELCNAYSELNDPIRQRELLEQQHTKKELLPDSECHPIDEEFLE
ALCQGMPPAGGFGIGVDRLVMILTNAASIRDVLYFPVMRRFDAEKTN
>Mature_526_residues
TARAEYLDHEDFLYRSHKLQELSELGVVLYPYEFPGVFSCEDIKKTFASQELGNSEAAMSRSTPRVRFAGRLVLFRAMGK
NAFGQILDHNQTIQVMFNREFTSVHGLSEDAEITPIKFIEKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLLSL
PDKHAGLSDKEVRYRKRWLDLISSREVSDTFVKRSYIIKLIRNYMDAHGFLEVETPILQNIYGGAEAKPFTTTMEALHSE
TFLRISLEIALKKILVGGAPRIYELGKVFRNEGIDRTHNPEFTMIEAYAAYMDYKEVMVFVENLVEHLVRAVNHDNTSLV
YSYWKHGPQEVDFKAPWIRMTMKESIATYAGIDVDVHSDQKLKEILKKKTTFPETAFATASRGMLIAALFDELVSDNLIA
PHHITDHPVETTPLCKTLRSGDTAFVERFESFCLGKELCNAYSELNDPIRQRELLEQQHTKKELLPDSECHPIDEEFLEA
LCQGMPPAGGFGIGVDRLVMILTNAASIRDVLYFPVMRRFDAEKTN

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=523, Percent_Identity=38.623326959847, Blast_Score=312, Evalue=6e-85,
Organism=Homo sapiens, GI194272210, Length=523, Percent_Identity=38.623326959847, Blast_Score=311, Evalue=7e-85,
Organism=Escherichia coli, GI1790571, Length=515, Percent_Identity=38.0582524271845, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789256, Length=506, Percent_Identity=38.3399209486166, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI87082379, Length=338, Percent_Identity=29.8816568047337, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788173, Length=206, Percent_Identity=28.1553398058252, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=469, Percent_Identity=40.2985074626866, Blast_Score=305, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI17535927, Length=469, Percent_Identity=40.2985074626866, Blast_Score=304, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI71994340, Length=469, Percent_Identity=40.2985074626866, Blast_Score=304, Evalue=8e-83,
Organism=Saccharomyces cerevisiae, GI6320242, Length=532, Percent_Identity=35.9022556390977, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6324256, Length=485, Percent_Identity=31.5463917525773, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24640849, Length=527, Percent_Identity=37.5711574952562, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24640851, Length=527, Percent_Identity=37.5711574952562, Blast_Score=313, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 60020; Mature: 59888

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARAEYLDHEDFLYRSHKLQELSELGVVLYPYEFPGVFSCEDIKKTFASQELGNSEAAM
CCCCCHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHH
SRSTPRVRFAGRLVLFRAMGKNAFGQILDHNQTIQVMFNREFTSVHGLSEDAEITPIKFI
CCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCCHHHHH
EKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLLSLPDKHAGLSDKEVRYRKRWL
HHHCCHHHEECCCCEEEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
DLISSREVSDTFVKRSYIIKLIRNYMDAHGFLEVETPILQNIYGGAEAKPFTTTMEALHS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCCCCCCHHHHHHHHHC
ETFLRISLEIALKKILVGGAPRIYELGKVFRNEGIDRTHNPEFTMIEAYAAYMDYKEVMV
CHHEEHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FVENLVEHLVRAVNHDNTSLVYSYWKHGPQEVDFKAPWIRMTMKESIATYAGIDVDVHSD
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCC
QKLKEILKKKTTFPETAFATASRGMLIAALFDELVSDNLIAPHHITDHPVETTPLCKTLR
HHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
SGDTAFVERFESFCLGKELCNAYSELNDPIRQRELLEQQHTKKELLPDSECHPIDEEFLE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ALCQGMPPAGGFGIGVDRLVMILTNAASIRDVLYFPVMRRFDAEKTN
HHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TARAEYLDHEDFLYRSHKLQELSELGVVLYPYEFPGVFSCEDIKKTFASQELGNSEAAM
CCCCHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHH
SRSTPRVRFAGRLVLFRAMGKNAFGQILDHNQTIQVMFNREFTSVHGLSEDAEITPIKFI
CCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCCHHHHH
EKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLLSLPDKHAGLSDKEVRYRKRWL
HHHCCHHHEECCCCEEEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
DLISSREVSDTFVKRSYIIKLIRNYMDAHGFLEVETPILQNIYGGAEAKPFTTTMEALHS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCCCCCCHHHHHHHHHC
ETFLRISLEIALKKILVGGAPRIYELGKVFRNEGIDRTHNPEFTMIEAYAAYMDYKEVMV
CHHEEHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FVENLVEHLVRAVNHDNTSLVYSYWKHGPQEVDFKAPWIRMTMKESIATYAGIDVDVHSD
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCC
QKLKEILKKKTTFPETAFATASRGMLIAALFDELVSDNLIAPHHITDHPVETTPLCKTLR
HHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
SGDTAFVERFESFCLGKELCNAYSELNDPIRQRELLEQQHTKKELLPDSECHPIDEEFLE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ALCQGMPPAGGFGIGVDRLVMILTNAASIRDVLYFPVMRRFDAEKTN
HHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]