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The map label for this gene is miaA

Identifier: 15836443

GI number: 15836443

Start: 1041300

End: 1042328

Strand: Direct

Name: miaA

Synonym: CPj0910

Alternate gene names: 15836443

Gene position: 1041300-1042328 (Clockwise)

Preceding gene: 15836442

Following gene: 15836445

Centisome position: 84.9

GC content: 38.78

Gene sequence:

>1029_bases
ATGCTTCCTTTTGAATTCGAGTTTAATACAACTTCTTCTCCTGAATGCGACGTATGCCTTGATCCTCAGAAGTTATTTGT
AAAGCTATTTAAGCGTACGATCGTCTTGCTTTCAGGACCTACAGGATCTGGGAAAACTGATGTTTCTTTAGCATTAGCCC
CCATGATTGATGGAGAGATCGTCTCAGTGGATTCTATGCAAGTATACCAAGGCATGGATATCGGAACTGCAAAAGTATCT
CTAAAAGCTAGACAAGAGATTCCTCACCATTTAATTGACATCCGACATGTTCAAGAGCCGTTTAATGTTGTGGATTTTTA
TTATGAAGCAATTCAAGCATGCCAAAACATTTTATCAAGAAATAAAGTTCCTATTTTAGTTGGTGGCTCAGGGTTTTATT
TTCATGCCTTTCTTTCAGGTCCTCCTAAAGGTCCCGCGGCAGATCCTCAGATACGAGAACAGCTTGAAGCGATAGCAGAA
GAACACGGGGTTTCTGCTCTTTATGAGGACCTACTTCTTAAAGATCCAGAATATGCTCAAACAATCACTAAGAATGATAA
AAATAAAATCATTCGAGGATTGGAAATTATCCAACTTACAGGAAAAAAAGTTAGCGATCATGAATGGGATATCGTTCCCA
AAGCTTCAAGAGAGTATTGTTGTCGTGCCTGGTTTCTTTCCCCTGAAACAGAATTTTTGAAAAATAATATTCAAATGCGT
TGCGAAGCTATGCTGCAAGAAGGGCTGCTAGAGGAAGTCAGGGGATTATTGAACCAGGGAATAAGGGAAAACCCTTCAGC
ATTCAAGGCCATTGGATATCGAGAATGGATAGAATTCCTTGATAACGGAGAGAAATTAGAAGAGTACGAGGAAACAAAAA
GAAAATTTGTATCCAATAGTTGGCATTATACTAAAAAACAAAAAACATGGTTTAAACGTTATTCGATATTTCGAGAACTT
CCGACATTAGGCCTCTCTTCGGACGCAATTGCTCAGAAGATAGCTAAAGACTACTTACTGTACAGCTGA

Upstream 100 bases:

>100_bases
AGAGAGTGTAACCGAAGTCATGCGGATTGCAGGTTTAGACCAATTGATCTTGCTCTGCCAGTCTGAACAGGAATGTTTAA
GTAAGTTATAGTTTCTCTAT

Downstream 100 bases:

>100_bases
TGTGTTGATAAAAGGTATTTCTCTCCACAGGAATGAAACCTTCAGATCGAATGATATTACAGATTTCTTCTTCAGAGCTC
TGTATCGACCATCCTGTAGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLPFEFEFNTTSSPECDVCLDPQKLFVKLFKRTIVLLSGPTGSGKTDVSLALAPMIDGEIVSVDSMQVYQGMDIGTAKVS
LKARQEIPHHLIDIRHVQEPFNVVDFYYEAIQACQNILSRNKVPILVGGSGFYFHAFLSGPPKGPAADPQIREQLEAIAE
EHGVSALYEDLLLKDPEYAQTITKNDKNKIIRGLEIIQLTGKKVSDHEWDIVPKASREYCCRAWFLSPETEFLKNNIQMR
CEAMLQEGLLEEVRGLLNQGIRENPSAFKAIGYREWIEFLDNGEKLEEYEETKRKFVSNSWHYTKKQKTWFKRYSIFREL
PTLGLSSDAIAQKIAKDYLLYS

Sequences:

>Translated_342_residues
MLPFEFEFNTTSSPECDVCLDPQKLFVKLFKRTIVLLSGPTGSGKTDVSLALAPMIDGEIVSVDSMQVYQGMDIGTAKVS
LKARQEIPHHLIDIRHVQEPFNVVDFYYEAIQACQNILSRNKVPILVGGSGFYFHAFLSGPPKGPAADPQIREQLEAIAE
EHGVSALYEDLLLKDPEYAQTITKNDKNKIIRGLEIIQLTGKKVSDHEWDIVPKASREYCCRAWFLSPETEFLKNNIQMR
CEAMLQEGLLEEVRGLLNQGIRENPSAFKAIGYREWIEFLDNGEKLEEYEETKRKFVSNSWHYTKKQKTWFKRYSIFREL
PTLGLSSDAIAQKIAKDYLLYS
>Mature_342_residues
MLPFEFEFNTTSSPECDVCLDPQKLFVKLFKRTIVLLSGPTGSGKTDVSLALAPMIDGEIVSVDSMQVYQGMDIGTAKVS
LKARQEIPHHLIDIRHVQEPFNVVDFYYEAIQACQNILSRNKVPILVGGSGFYFHAFLSGPPKGPAADPQIREQLEAIAE
EHGVSALYEDLLLKDPEYAQTITKNDKNKIIRGLEIIQLTGKKVSDHEWDIVPKASREYCCRAWFLSPETEFLKNNIQMR
CEAMLQEGLLEEVRGLLNQGIRENPSAFKAIGYREWIEFLDNGEKLEEYEETKRKFVSNSWHYTKKQKTWFKRYSIFREL
PTLGLSSDAIAQKIAKDYLLYS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=320, Percent_Identity=30, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1790613, Length=285, Percent_Identity=32.9824561403509, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25144712, Length=296, Percent_Identity=27.027027027027, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324848, Length=301, Percent_Identity=30.5647840531561, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24649830, Length=288, Percent_Identity=31.5972222222222, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHLPN (Q9Z6Z6)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D86604
- PIR:   E72019
- RefSeq:   NP_225105.1
- RefSeq:   NP_300967.1
- RefSeq:   NP_445493.1
- RefSeq:   NP_877214.1
- ProteinModelPortal:   Q9Z6Z6
- SMR:   Q9Z6Z6
- GeneID:   1467621
- GeneID:   895662
- GeneID:   919675
- GeneID:   963421
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0956
- KEGG:   cpn:CPn0910
- KEGG:   cpt:CpB0943
- TIGR:   CP_0956
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q9Z6Z6
- ProtClustDB:   PRK00091
- BioCyc:   CPNE115711:CP_0956-MONOMER
- BioCyc:   CPNE115713:CPN0910-MONOMER
- BioCyc:   CPNE138677:CPJ0910-MONOMER
- BioCyc:   CPNE182082:CPB0943-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 39069; Mature: 39069

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPFEFEFNTTSSPECDVCLDPQKLFVKLFKRTIVLLSGPTGSGKTDVSLALAPMIDGEI
CCCEEEECCCCCCCCCCEEECHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCCCE
VSVDSMQVYQGMDIGTAKVSLKARQEIPHHLIDIRHVQEPFNVVDFYYEAIQACQNILSR
EEECCHHHHCCCCCCHHHEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NKVPILVGGSGFYFHAFLSGPPKGPAADPQIREQLEAIAEEHGVSALYEDLLLKDPEYAQ
CCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHH
TITKNDKNKIIRGLEIIQLTGKKVSDHEWDIVPKASREYCCRAWFLSPETEFLKNNIQMR
HHHCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
CEAMLQEGLLEEVRGLLNQGIRENPSAFKAIGYREWIEFLDNGEKLEEYEETKRKFVSNS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
WHYTKKQKTWFKRYSIFRELPTLGLSSDAIAQKIAKDYLLYS
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPFEFEFNTTSSPECDVCLDPQKLFVKLFKRTIVLLSGPTGSGKTDVSLALAPMIDGEI
CCCEEEECCCCCCCCCCEEECHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCCCE
VSVDSMQVYQGMDIGTAKVSLKARQEIPHHLIDIRHVQEPFNVVDFYYEAIQACQNILSR
EEECCHHHHCCCCCCHHHEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NKVPILVGGSGFYFHAFLSGPPKGPAADPQIREQLEAIAEEHGVSALYEDLLLKDPEYAQ
CCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHH
TITKNDKNKIIRGLEIIQLTGKKVSDHEWDIVPKASREYCCRAWFLSPETEFLKNNIQMR
HHHCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
CEAMLQEGLLEEVRGLLNQGIRENPSAFKAIGYREWIEFLDNGEKLEEYEETKRKFVSNS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
WHYTKKQKTWFKRYSIFRELPTLGLSSDAIAQKIAKDYLLYS
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362