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The map label for this gene is lpdA

Identifier: 15836366

GI number: 15836366

Start: 939065

End: 940450

Strand: Reverse

Name: lpdA

Synonym: CPj0833

Alternate gene names: 15836366

Gene position: 940450-939065 (Counterclockwise)

Preceding gene: 15836368

Following gene: 15836365

Centisome position: 76.67

GC content: 43.94

Gene sequence:

>1386_bases
ATGACCCAAGAATTTGATTGTGTTGTTATTGGTGCGGGACCTAGTGGCTATGTTGCCGCAATCACTGCTGCGCAATCAAA
ATTACGGACCGCTCTTATTGAAGAAGACCAGGCTGGGGGGACCTGCTTAAACCGCGGATGCATCCCTTCAAAAGCCCTCA
TTGCTGGAGCCAATGTTGTATCTCACATTAAGCATGCGGAGCAGTTCGGCATCCATGTTGATGGTTATACAATCGATTAC
CCTGCGATGGCAAAAAGAAAAAATACAGTCGTCCAGGGGATCCGTCAAGGATTAGAAGGATTGATCCGCAGCAACAAGAT
TACTGTCTTAAAAGGAACCGGATCTCTAGTATCTTCTACAGAAGTTAAAGTTATTGGCCAAGACACGACTATAATCAAAG
CCAATCATATTATCCTAGCTACAGGATCCGAGCCTCGTCCTTTCCCAGGGGTTCCCTTCTCCTCTAGAATTTTGAGTTCC
ACAGGGATCTTAGAACTTGAAGTCCTCCCTAAAAAGCTCGCTATTATTGGTGGCGGCGTTATTGGCTGTGAATTTGCGTC
TCTATTTCACACTTTAGGCGTTGAGATTACCGTTATAGAAGCTTTGGATCATATTCTTGCGGTTAACAATAAAGAAGTTT
CTCAAACCGTAACGAATAAATTTACGAAACAAGGAATTCGAATTCTTACCAAAGCCTCGATCTCTGCAATCGAAGAATCC
CAAAACCAAGTTCGCATTACTGTGAACGATCAAGTGGAAGAGTTTGATTATGTCTTGGTGGCTATTGGTCGCCAATTTAA
TACAGCAAGTATAGGGCTAGATAATGCTGGAGTGATCCGGGACGATCGTGGCGTGATTCCTGTTGACGAAACCATGCGCA
CTAATGTTCCAAATATCTATGCGATTGGAGACATCACTGGAAAGTGGCTACTTGCTCATGTGGCTTCGCACCAAGGCGTT
ATTGCCGCGAAAAATATTTCGGGACATCACGAAGTTATGGATTATTCTGCCATACCTTCTGTGATCTTTACCCACCCAGA
AATTGCTATGGTAGGTCTATCTCTACAAGAAGCAGAACAACAAAATCTTCCTGCAAAGCTCACCAAATTTCCTTTTAAAG
CGATTGGAAAAGCTGTTGCTTTGGGAGCATCTGATGGTTTTGCTGCTATTGTGAGTCATGAAATTACCCAGCAAATACTC
GGAGCTTATGTCATAGGACCTCACGCCTCATCATTAATTGGAGAGATGACCTTAGCGATCCGCAATGAGCTGACCCTACC
TTGCATATATGAAACCGTGCATGCTCATCCCACACTCTCTGAAGTTTGGGCTGAAGGTGCTTTACTTGCTACAAATCACC
CTTTACACTTCCCTCCTAAGTCATGA

Upstream 100 bases:

>100_bases
TCTTTAGGTCTAAAATAAGCACAGGGTCCTCTTCTTAATGAGATGCATGCATTTATGAAGGGGGGTTCTTTTTGAAATCT
TGATTACAGTGAGTGAGAAT

Downstream 100 bases:

>100_bases
AATGTAGACCAACTTTAAATACAGACCAACCTAGAGTGCGGAAAAAGCTTCCCGAGCGTTTCCCCAAGTGGTTACAGAGG
CCCTTACCACAAGGTTCTGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTQEFDCVVIGAGPSGYVAAITAAQSKLRTALIEEDQAGGTCLNRGCIPSKALIAGANVVSHIKHAEQFGIHVDGYTIDY
PAMAKRKNTVVQGIRQGLEGLIRSNKITVLKGTGSLVSSTEVKVIGQDTTIIKANHIILATGSEPRPFPGVPFSSRILSS
TGILELEVLPKKLAIIGGGVIGCEFASLFHTLGVEITVIEALDHILAVNNKEVSQTVTNKFTKQGIRILTKASISAIEES
QNQVRITVNDQVEEFDYVLVAIGRQFNTASIGLDNAGVIRDDRGVIPVDETMRTNVPNIYAIGDITGKWLLAHVASHQGV
IAAKNISGHHEVMDYSAIPSVIFTHPEIAMVGLSLQEAEQQNLPAKLTKFPFKAIGKAVALGASDGFAAIVSHEITQQIL
GAYVIGPHASSLIGEMTLAIRNELTLPCIYETVHAHPTLSEVWAEGALLATNHPLHFPPKS

Sequences:

>Translated_461_residues
MTQEFDCVVIGAGPSGYVAAITAAQSKLRTALIEEDQAGGTCLNRGCIPSKALIAGANVVSHIKHAEQFGIHVDGYTIDY
PAMAKRKNTVVQGIRQGLEGLIRSNKITVLKGTGSLVSSTEVKVIGQDTTIIKANHIILATGSEPRPFPGVPFSSRILSS
TGILELEVLPKKLAIIGGGVIGCEFASLFHTLGVEITVIEALDHILAVNNKEVSQTVTNKFTKQGIRILTKASISAIEES
QNQVRITVNDQVEEFDYVLVAIGRQFNTASIGLDNAGVIRDDRGVIPVDETMRTNVPNIYAIGDITGKWLLAHVASHQGV
IAAKNISGHHEVMDYSAIPSVIFTHPEIAMVGLSLQEAEQQNLPAKLTKFPFKAIGKAVALGASDGFAAIVSHEITQQIL
GAYVIGPHASSLIGEMTLAIRNELTLPCIYETVHAHPTLSEVWAEGALLATNHPLHFPPKS
>Mature_460_residues
TQEFDCVVIGAGPSGYVAAITAAQSKLRTALIEEDQAGGTCLNRGCIPSKALIAGANVVSHIKHAEQFGIHVDGYTIDYP
AMAKRKNTVVQGIRQGLEGLIRSNKITVLKGTGSLVSSTEVKVIGQDTTIIKANHIILATGSEPRPFPGVPFSSRILSST
GILELEVLPKKLAIIGGGVIGCEFASLFHTLGVEITVIEALDHILAVNNKEVSQTVTNKFTKQGIRILTKASISAIEESQ
NQVRITVNDQVEEFDYVLVAIGRQFNTASIGLDNAGVIRDDRGVIPVDETMRTNVPNIYAIGDITGKWLLAHVASHQGVI
AAKNISGHHEVMDYSAIPSVIFTHPEIAMVGLSLQEAEQQNLPAKLTKFPFKAIGKAVALGASDGFAAIVSHEITQQILG
AYVIGPHASSLIGEMTLAIRNELTLPCIYETVHAHPTLSEVWAEGALLATNHPLHFPPKS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=37.0932754880694, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=28.1045751633987, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI22035672, Length=432, Percent_Identity=28.4722222222222, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI291045266, Length=428, Percent_Identity=26.4018691588785, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI148277071, Length=436, Percent_Identity=26.3761467889908, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI148277065, Length=436, Percent_Identity=26.3761467889908, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519430, Length=436, Percent_Identity=26.3761467889908, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519428, Length=436, Percent_Identity=26.3761467889908, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519426, Length=436, Percent_Identity=26.3761467889908, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI291045268, Length=422, Percent_Identity=24.1706161137441, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786307, Length=442, Percent_Identity=35.972850678733, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=29.2841648590022, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=28.6353467561521, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789915, Length=448, Percent_Identity=28.5714285714286, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1789065, Length=242, Percent_Identity=27.6859504132231, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=37.9828326180258, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17557007, Length=469, Percent_Identity=27.5053304904051, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI71983419, Length=365, Percent_Identity=26.3013698630137, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=365, Percent_Identity=26.3013698630137, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=344, Percent_Identity=24.4186046511628, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=194, Percent_Identity=27.319587628866, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=476, Percent_Identity=36.5546218487395, Blast_Score=275, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=28.5097192224622, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=27.061310782241, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=40.7643312101911, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI24640549, Length=474, Percent_Identity=28.0590717299578, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640553, Length=474, Percent_Identity=28.0590717299578, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640551, Length=474, Percent_Identity=28.4810126582279, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=27.6150627615063, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH_CHLPN (Q9Z773)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AB035943
- EMBL:   AE009440
- PIR:   C72031
- PIR:   G86594
- RefSeq:   NP_225028.1
- RefSeq:   NP_300890.1
- RefSeq:   NP_445574.1
- RefSeq:   NP_877134.1
- ProteinModelPortal:   Q9Z773
- SMR:   Q9Z773
- PHCI-2DPAGE:   Q9Z773
- GeneID:   1467541
- GeneID:   895532
- GeneID:   919599
- GeneID:   963515
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP1037
- KEGG:   cpn:CPn0833
- KEGG:   cpt:CpB0862
- TIGR:   CP_1037
- HOGENOM:   HBG515043
- OMA:   PWEVAHA
- PhylomeDB:   Q9Z773
- ProtClustDB:   PRK06327
- BioCyc:   CPNE115711:CP_1037-MONOMER
- BioCyc:   CPNE115713:CPN0833-MONOMER
- BioCyc:   CPNE138677:CPJ0833-MONOMER
- BioCyc:   CPNE182082:CPB0862-MONOMER
- BRENDA:   1.8.1.4
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 49167; Mature: 49036

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 436-436 BINDING 51-51 BINDING 114-114 BINDING 200-200 BINDING 304-304 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEFDCVVIGAGPSGYVAAITAAQSKLRTALIEEDQAGGTCLNRGCIPSKALIAGANVV
CCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHH
SHIKHAEQFGIHVDGYTIDYPAMAKRKNTVVQGIRQGLEGLIRSNKITVLKGTGSLVSST
HHHHHHHHCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
EVKVIGQDTTIIKANHIILATGSEPRPFPGVPFSSRILSSTGILELEVLPKKLAIIGGGV
EEEEEECCEEEEEECEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEECCCH
IGCEFASLFHTLGVEITVIEALDHILAVNNKEVSQTVTNKFTKQGIRILTKASISAIEES
HHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QNQVRITVNDQVEEFDYVLVAIGRQFNTASIGLDNAGVIRDDRGVIPVDETMRTNVPNIY
CCEEEEEECCCHHHHCEEEEEECCCCCEEEECCCCCCEEECCCCCCCCCHHHHCCCCCEE
AIGDITGKWLLAHVASHQGVIAAKNISGHHEVMDYSAIPSVIFTHPEIAMVGLSLQEAEQ
EEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCHHEECCCCEEEEECCHHHHHH
QNLPAKLTKFPFKAIGKAVALGASDGFAAIVSHEITQQILGAYVIGPHASSLIGEMTLAI
HCCCHHHHHCCHHHHCCEEEECCCCCHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHH
RNELTLPCIYETVHAHPTLSEVWAEGALLATNHPLHFPPKS
HCCCCCHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
TQEFDCVVIGAGPSGYVAAITAAQSKLRTALIEEDQAGGTCLNRGCIPSKALIAGANVV
CCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHH
SHIKHAEQFGIHVDGYTIDYPAMAKRKNTVVQGIRQGLEGLIRSNKITVLKGTGSLVSST
HHHHHHHHCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
EVKVIGQDTTIIKANHIILATGSEPRPFPGVPFSSRILSSTGILELEVLPKKLAIIGGGV
EEEEEECCEEEEEECEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEECCCH
IGCEFASLFHTLGVEITVIEALDHILAVNNKEVSQTVTNKFTKQGIRILTKASISAIEES
HHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QNQVRITVNDQVEEFDYVLVAIGRQFNTASIGLDNAGVIRDDRGVIPVDETMRTNVPNIY
CCEEEEEECCCHHHHCEEEEEECCCCCEEEECCCCCCEEECCCCCCCCCHHHHCCCCCEE
AIGDITGKWLLAHVASHQGVIAAKNISGHHEVMDYSAIPSVIFTHPEIAMVGLSLQEAEQ
EEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCHHEECCCCEEEEECCHHHHHH
QNLPAKLTKFPFKAIGKAVALGASDGFAAIVSHEITQQILGAYVIGPHASSLIGEMTLAI
HCCCHHHHHCCHHHHCCEEEECCCCCHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHH
RNELTLPCIYETVHAHPTLSEVWAEGALLATNHPLHFPPKS
HCCCCCHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362