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The map label for this gene is nfo

Identifier: 15836264

GI number: 15836264

Start: 821419

End: 822300

Strand: Direct

Name: nfo

Synonym: CPj0732

Alternate gene names: 15836264

Gene position: 821419-822300 (Clockwise)

Preceding gene: 15836263

Following gene: 15836266

Centisome position: 66.97

GC content: 40.59

Gene sequence:

>882_bases
ATGAAAGTACTTCCTCCTCCCTCCATTCCCTTACTAGGGGCTCACACTTCAACTGCTGGTGGACTCAAAAATGCGATTTA
TGAAGGCCGGGATATAGGGGCTTCTACAGTTCAGATTTTTACAGCAAACCAAAGGCAGTGGCAAAGACGGGCTCTAAAAG
AAGAAGTGATTGAAGATTTCAAAGCAGCGCTCAAAGAAACTGACCTTTCTTATATTATGAGTCATGCAGGATATCTGATT
AATCCAGGAGCCCCTGATCCGGTAATTTTAGAAAAAAGTCGGATTGGCATTTATCAAGAAATTCTGGACTGCATCACTTT
AGGCATTTCTTTTGTTAATTTTCACCCTGGAGCAGCTCTCAAAAGCTCTAAAGAAGACTGCATGAATAAAATTGTCAGCA
GTTTTAGCCAATCGGCCCCTTTATTTGATAGTTCTCCTCCTCTTGTTGTTTTACTGGAAACCACAGCGGGTCAGGGAACG
TTAATTGGGAGTAACTTTGAAGAATTGGGTTACCTCGTTCAGAATTTGAAAAATCAAATTCCCATTGGCGTGTGTGTAGA
TACTTGTCATATTTTTGCTGCGGGGTACGACATTACCTCTCCACAGGGGTGGGAAGATGTTCTTAATGAATTTGACGAGT
ATGTCGGTTTATCTTATCTACGAGCCTTTCATCTCAATGATTCTATGTTTCCATTAGGAGCGAACAAAGACCGCCATGCG
CCCCTTGGAGAGGGCTATATAGGTAAGGAATCTTTTAAATTTTTAATGACAGATGAACGAACTAGAAAAATTCCTAAGTA
TTTAGAAACCCCTGGTGGGCCTGAAAATTGGCAAAAAGAAATTGGGGAACTTTTGAAGTTTTCAAAAAACAGAGATAGTT
AG

Upstream 100 bases:

>100_bases
ATTTTTGAAAAATTGAAAAAGCCTCAAGAAATGAGCAGCTCAATCAAAGGACCTAGGTTTCCTCTGAAACTGGGTAGTTA
AGAAAAGACCTTGAAATTTT

Downstream 100 bases:

>100_bases
GAAGTTTTTTAAGTGCTTTTAGATCCCGAAGCAATCCAGTAGATCTTCTGAAATCAAAAAAAACGCCATACTGATATACA
GTTGGCGTTTTCTAGAAAAG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKVLPPPSIPLLGAHTSTAGGLKNAIYEGRDIGASTVQIFTANQRQWQRRALKEEVIEDFKAALKETDLSYIMSHAGYLI
NPGAPDPVILEKSRIGIYQEILDCITLGISFVNFHPGAALKSSKEDCMNKIVSSFSQSAPLFDSSPPLVVLLETTAGQGT
LIGSNFEELGYLVQNLKNQIPIGVCVDTCHIFAAGYDITSPQGWEDVLNEFDEYVGLSYLRAFHLNDSMFPLGANKDRHA
PLGEGYIGKESFKFLMTDERTRKIPKYLETPGGPENWQKEIGELLKFSKNRDS

Sequences:

>Translated_293_residues
MKVLPPPSIPLLGAHTSTAGGLKNAIYEGRDIGASTVQIFTANQRQWQRRALKEEVIEDFKAALKETDLSYIMSHAGYLI
NPGAPDPVILEKSRIGIYQEILDCITLGISFVNFHPGAALKSSKEDCMNKIVSSFSQSAPLFDSSPPLVVLLETTAGQGT
LIGSNFEELGYLVQNLKNQIPIGVCVDTCHIFAAGYDITSPQGWEDVLNEFDEYVGLSYLRAFHLNDSMFPLGANKDRHA
PLGEGYIGKESFKFLMTDERTRKIPKYLETPGGPENWQKEIGELLKFSKNRDS
>Mature_293_residues
MKVLPPPSIPLLGAHTSTAGGLKNAIYEGRDIGASTVQIFTANQRQWQRRALKEEVIEDFKAALKETDLSYIMSHAGYLI
NPGAPDPVILEKSRIGIYQEILDCITLGISFVNFHPGAALKSSKEDCMNKIVSSFSQSAPLFDSSPPLVVLLETTAGQGT
LIGSNFEELGYLVQNLKNQIPIGVCVDTCHIFAAGYDITSPQGWEDVLNEFDEYVGLSYLRAFHLNDSMFPLGANKDRHA
PLGEGYIGKESFKFLMTDERTRKIPKYLETPGGPENWQKEIGELLKFSKNRDS

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=279, Percent_Identity=41.2186379928315, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17531193, Length=269, Percent_Identity=43.1226765799257, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322735, Length=263, Percent_Identity=35.361216730038, Blast_Score=180, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_CHLPN (Q9Z7H3)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A86582
- PIR:   H72042
- RefSeq:   NP_224928.1
- RefSeq:   NP_300788.1
- RefSeq:   NP_444566.1
- RefSeq:   NP_877032.1
- ProteinModelPortal:   Q9Z7H3
- SMR:   Q9Z7H3
- GeneID:   1467439
- GeneID:   895543
- GeneID:   919495
- GeneID:   963636
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0014
- KEGG:   cpn:CPn0732
- KEGG:   cpt:CpB0760
- TIGR:   CP_0014
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   Q9Z7H3
- ProtClustDB:   PRK01060
- BioCyc:   CPNE115711:CP_0014-MONOMER
- BioCyc:   CPNE115713:CPN0732-MONOMER
- BioCyc:   CPNE138677:CPJ0732-MONOMER
- BioCyc:   CPNE182082:CPB0760-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 32389; Mature: 32389

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLPPPSIPLLGAHTSTAGGLKNAIYEGRDIGASTVQIFTANQRQWQRRALKEEVIEDF
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
KAALKETDLSYIMSHAGYLINPGAPDPVILEKSRIGIYQEILDCITLGISFVNFHPGAAL
HHHHHHHHHHHHHHHCCEEECCCCCCCEEEECHHCCHHHHHHHHHHHHHHHHCCCCCCHH
KSSKEDCMNKIVSSFSQSAPLFDSSPPLVVLLETTAGQGTLIGSNFEELGYLVQNLKNQI
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHCC
PIGVCVDTCHIFAAGYDITSPQGWEDVLNEFDEYVGLSYLRAFHLNDSMFPLGANKDRHA
CEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PLGEGYIGKESFKFLMTDERTRKIPKYLETPGGPENWQKEIGELLKFSKNRDS
CCCCCCCCHHHHHEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVLPPPSIPLLGAHTSTAGGLKNAIYEGRDIGASTVQIFTANQRQWQRRALKEEVIEDF
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
KAALKETDLSYIMSHAGYLINPGAPDPVILEKSRIGIYQEILDCITLGISFVNFHPGAAL
HHHHHHHHHHHHHHHCCEEECCCCCCCEEEECHHCCHHHHHHHHHHHHHHHHCCCCCCHH
KSSKEDCMNKIVSSFSQSAPLFDSSPPLVVLLETTAGQGTLIGSNFEELGYLVQNLKNQI
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHCC
PIGVCVDTCHIFAAGYDITSPQGWEDVLNEFDEYVGLSYLRAFHLNDSMFPLGANKDRHA
CEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PLGEGYIGKESFKFLMTDERTRKIPKYLETPGGPENWQKEIGELLKFSKNRDS
CCCCCCCCHHHHHEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362