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The map label for this gene is hisS

Identifier: 15836195

GI number: 15836195

Start: 742219

End: 743511

Strand: Reverse

Name: hisS

Synonym: CPj0663

Alternate gene names: 15836195

Gene position: 743511-742219 (Counterclockwise)

Preceding gene: 15836196

Following gene: 15836194

Centisome position: 60.62

GC content: 41.14

Gene sequence:

>1293_bases
GTGACTGTAACTCTCCCCAAAGGGGTCTTTGATATATTTCCTTATCTTGCAGATGCTAAACAATTGTGGCGTCACACTTC
ACTTTGGCATAGTGTTGAGAAGGCAATTCATACCGTTTGCATGCTTTATGGATTTTGTGAAATTCGTACTCCTATTTTTG
AAAAATCAGAAGTGTTTTTACATGTAGGGGAAGAAAGTGATGTTGTTAAAAAAGAAGTCTATTCGGTTTTAGATAGAAAA
GGTCGTTCCATGACTTTGCGTCCCGAAAGGACTGCCGCTGTTGTCAGTGCTTTTCTTGAACACGGGGCCTCTCATCGAAG
TGATATTAAGTTCTATTATATTCTTCCCATGTTTCGCTACGAACGTCAGCAAGCGGGTAGATATCGTCAACACCATCAGT
TCGGTGTTGAGGCTATTGGTGTGCGTCACCCTCTAAGAGATGCAGAAGTTCTCGCTTTGCTTTGGGATTTCTACTCTCGT
GTCGGTTTGCAACATATGCAAATTCAACTCAATTTCTTAGGAGGAAGTGAGACAAGATTTCGATACGATAAGGTTCTACG
CGCTTATTTGAAAGAGTCCATGGGAGAATTATCGGCATTAAGCCAGCAGAGATTTTCAACGAATGTTTTGCGTATTTTGG
ATTCAAAGGAGCCTGAAGATCAAGAAATTATCCGTCAAGCGCCCCCGATTCTAGACTATGTTTCTGATGAAGATCTTAAG
TATTTTAATGAAATTTTAGACGCTTTGAGAGTTTTAGAAATCCCATATGCTATCAATCCTCGTTTAGTGCGTGGTTTGGA
TTATTATTCAGACTTAGTCTTTGAAGCGACTACCACATTCCAAGAGGTCTCTTATGCCTTAGGGGGAGGCGGGCGCTATG
ACGGCTTGATTTCAGCTTTTGGAGGAGCTTCTCTTCCTGCCTGTGGTTTCGGTGTTGGCCTTGAAAGAGCGATTCAAACG
TTATTAGCTCAAAAGCGTATTGAGCCACAGTTCCCTCATAAACTGCGTTTGATTCCAATGGAACCAGATGCGGATCAGTT
TTGTTTGGAATGGTCGCAACATTTGCGACGGTTGGGAATCCCTACGGAAGTCGATTGGTCTCATAAAAAAGTAAAAGGAG
CTCTAAAAGCAGCGAGTACAGAACAGGTTTCTTTCGTTTGCTTAATCGGTGAGCGAGAGTTAATTTCTCAACAGTTAGTT
ATTAAAAATATGTCTCTGCGTAAAGAATTCTTTGGAACAAAAGAAGAAGTAGAGCAAAGGTTGTTATATGAAATACAGAA
CACACCGTTGTAA

Upstream 100 bases:

>100_bases
GTAGATTCGAGTGTGAATATGACTTAGGCTTAGGCAGGAGTCACTAAGCTCCAGATATTCCATTATCTAAAAAAGTAATC
ATTTTGAGCGAAGACATCAC

Downstream 100 bases:

>100_bases
TGAACTCACAAGTAATCATATAGGTGAGAATGTTCAATTGGCAGGATGGGTGCACCGTTATCGTAATCATGGGGGTGTTG
TCTTCATAGATTTGCGAGAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFLHVGEESDVVKKEVYSVLDRK
GRSMTLRPERTAAVVSAFLEHGASHRSDIKFYYILPMFRYERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSR
VGLQHMQIQLNFLGGSETRFRYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLK
YFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAFGGASLPACGFGVGLERAIQT
LLAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGIPTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLV
IKNMSLRKEFFGTKEEVEQRLLYEIQNTPL

Sequences:

>Translated_430_residues
MTVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFLHVGEESDVVKKEVYSVLDRK
GRSMTLRPERTAAVVSAFLEHGASHRSDIKFYYILPMFRYERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSR
VGLQHMQIQLNFLGGSETRFRYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLK
YFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAFGGASLPACGFGVGLERAIQT
LLAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGIPTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLV
IKNMSLRKEFFGTKEEVEQRLLYEIQNTPL
>Mature_429_residues
TVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFLHVGEESDVVKKEVYSVLDRKG
RSMTLRPERTAAVVSAFLEHGASHRSDIKFYYILPMFRYERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSRV
GLQHMQIQLNFLGGSETRFRYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLKY
FNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAFGGASLPACGFGVGLERAIQTL
LAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGIPTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLVI
KNMSLRKEFFGTKEEVEQRLLYEIQNTPL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=387, Percent_Identity=36.6925064599483, Blast_Score=250, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 49517; Mature: 49385

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFL
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
HVGEESDVVKKEVYSVLDRKGRSMTLRPERTAAVVSAFLEHGASHRSDIKFYYILPMFRY
EECCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHH
ERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSRVGLQHMQIQLNFLGGSETRF
HHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHH
RYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHCCCHHHH
YFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAF
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
GGASLPACGFGVGLERAIQTLLAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
PTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLVIKNMSLRKEFFGTKEEVEQR
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLYEIQNTPL
HHHHHHCCCC
>Mature Secondary Structure 
TVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
HVGEESDVVKKEVYSVLDRKGRSMTLRPERTAAVVSAFLEHGASHRSDIKFYYILPMFRY
EECCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHH
ERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSRVGLQHMQIQLNFLGGSETRF
HHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHH
RYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHCCCHHHH
YFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAF
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
GGASLPACGFGVGLERAIQTLLAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
PTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLVIKNMSLRKEFFGTKEEVEQR
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLYEIQNTPL
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]