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The map label for this gene is rpoA

Identifier: 15836158

GI number: 15836158

Start: 717813

End: 718937

Strand: Reverse

Name: rpoA

Synonym: CPj0626

Alternate gene names: 15836158

Gene position: 718937-717813 (Counterclockwise)

Preceding gene: 15836159

Following gene: 15836157

Centisome position: 58.61

GC content: 36.53

Gene sequence:

>1125_bases
ATGTCAGATAACGCACACAATTTACTTTATGATAAGTTTGAACTGCCTGAAGCAGTTAAAATGTTGCCTGTTGAAGGGCT
TCCAATAGACAAACATGCTCGCTTCATTGCTGAGCCTCTAGAAAGAGGAATGGGCCACACTTTAGGAAATGCTCTAAGAC
GTGCTTTGCTTATTGGTTTAGAAGCTCCAGCTATTATCTCGTTTGCTATGACAGGCGTACTTCATGAATATATGGCAATC
GAAGGGGTTATTGAGGATGTAACTAACATCATTCTGAATTTAAAAGGTGCCCTATTAAAAAAGTACCCCATGCAGGATAG
TTCTTTAGGAAGAACCACTCAGGTCTTAAAAGCTTCAATTTCTATAGATGCTTCCGATTTAGCCGCAGCTAATGGACAAA
AAGAAGTGACTCTACAAGATCTATTGCAAGAAGGGGACTTTGAAGCCGTTAACCCAGATCAAGTCATTTTTACTGTTACT
CAACCCATACAGTTGGAAGTCGTTCTACGAATTGCTTTTGGTAGGGGATATACACCTTCTGAAAGGATTGTTTTGGAAGA
TAAGGGTGTTTATGAAATCGTTTTGGATGCAGCCTTTTCTCCAGTCACTTTAGTCAACTACTTTGTAGAAGATACACGGG
TGGGTCAGGATACAGATTTTGACCGTTTAGTTTTAATAGTTGAAACAGATGGAAGAGTGACTCCGAAAGAAGCTCTGGCT
TTTTCAACTCAAATTTTGACTAAACATTTTTCCATTTTTGAAAATATGGATGAGAAGAAAATCGTATTTGAAGAAGCTAT
TTCTATTGAGAAAGAAAACAAAGATGATATTCTTCATAAGTTAATTTTAGGAATTAATGAAATAGAACTCTCAGTCAGAT
CAACAAATTGTTTGTCTAATGCAAATATTGAGACTATTGGCGAACTTGTTATTATGCCTGAACCTCGATTGCTACAATTC
AGAAATTTTGGAAAGAAATCACTGTGTGAGATCAAGAATAAATTGAAAGAAATGAAGCTTGAATTAGGAATGGACCTAAC
GCAATTTGGCGTAGGTTTAGATAACGTAAAAGAAAAAATGAAGTGGTATGCCGAAAAGATTCGGGCTAAAAATATAAAGG
GATAG

Upstream 100 bases:

>100_bases
GTTTGGTAGTTTCTGTAATCCGTGATGAAACTCCTGTTCCTCATAATGGTTGCCGGCCAAGAAAAAGGCGCAGAGTGTAG
TTTTAGGGAAGGAGAAAGGG

Downstream 100 bases:

>100_bases
TAAGTAATGCAACACGCTAGAAAAAAATTTAGAGTTGGTCGTACTTCCTCGCATAATCGCTGTATGTTAGCTAACATGTT
AAAGTCTCTAATTCATTATG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFAMTGVLHEYMAI
EGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVT
QPIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALA
FSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQF
RNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNIKG

Sequences:

>Translated_374_residues
MSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFAMTGVLHEYMAI
EGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVT
QPIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALA
FSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQF
RNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNIKG
>Mature_373_residues
SDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFAMTGVLHEYMAIE
GVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVTQ
PIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALAF
STQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQFR
NFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNIKG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=322, Percent_Identity=38.5093167701863, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CHLPN (Q9Z7S8)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D72053
- PIR:   E81613
- PIR:   G86568
- RefSeq:   NP_224822.1
- RefSeq:   NP_300682.1
- RefSeq:   NP_444673.1
- RefSeq:   NP_876924.1
- ProteinModelPortal:   Q9Z7S8
- PHCI-2DPAGE:   Q9Z7S8
- GeneID:   1467331
- GeneID:   895399
- GeneID:   919391
- GeneID:   963000
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0121
- KEGG:   cpn:CPn0626
- KEGG:   cpt:CpB0652
- TIGR:   CP_0121
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   CPNE115711:CP_0121-MONOMER
- BioCyc:   CPNE115713:CPN0626-MONOMER
- BioCyc:   CPNE138677:CPJ0626-MONOMER
- BioCyc:   CPNE182082:CPB0652-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 41875; Mature: 41744

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGL
CCCCCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
EAPAIISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
SIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVTQPIQLEVVLRIAFGRGYTPS
CCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCC
ERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALA
CEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHH
FSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
ANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKM
CCHHHHCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
KWYAEKIRAKNIKG
HHHHHHHHHCCCCC
>Mature Secondary Structure 
SDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGL
CCCCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
EAPAIISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
SIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVTQPIQLEVVLRIAFGRGYTPS
CCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCC
ERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLVLIVETDGRVTPKEALA
CEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHH
FSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
ANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKM
CCHHHHCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
KWYAEKIRAKNIKG
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362