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The map label for this gene is dnaB

Identifier: 15836148

GI number: 15836148

Start: 708112

End: 709461

Strand: Direct

Name: dnaB

Synonym: CPj0616

Alternate gene names: 15836148

Gene position: 708112-709461 (Clockwise)

Preceding gene: 15836141

Following gene: 15836149

Centisome position: 57.73

GC content: 39.63

Gene sequence:

>1350_bases
ATGATAGTTTTAGGTTGCATGCTGACAGGGGTACATTATCTAAATCTTGCAGCCAACCAACTCTACGAAGAAGATTTTTA
TTACCTTGAACATAAAATTATTTTTCGAGTCCTCCAAGATGCCTTCAAGCAAGATAAACCTATCGATGTTCACTTAGCTG
GAGAAGAACTCAAACGGCACAACCAGATTACTGTAATTGGGGGCCCTTCGTATCTAATTACTTTAGCCGAATTTGCAGGT
ACCGCAGCCTATCTTGAAGAATACGTAGACATCATCCGATCGAAGTCGATTCTAAGGAAGATGATTTCTACAGCAAAAGA
AATCGAAAAAAGAGCTTTAGAGCAGCCGAAAAATGTTGCCGAAGCCTTAGATGAAGCTCAGAATTCTTTTTTTAAAATCA
GCCAATCAACATCGGTAAGTCAGTACACTTTAGTTGCTGACAAATTACGCGGGTTAACAACAACTACAGATAAGCCTTAC
CTTGTACAATTACAAGAGAGACAAGAATTATTTTTACAGAATGCTCAAGGAGACAATAAGTCTTTCTTCACTGGCATTCC
CACACACTTTATTGATTTAGACCAGCTGATTCACGGATTTTCTCCTTCAAATTTGATGATCTTAGCTGCCCGCCCTGCCA
TGGGGAAAACAGCACTCGCTTTGAATATTGCAGAGAATCTTTGTTTTCAAAACCGCCTCCCCATTGGAATTTTTTCTTTA
GAGATGACAGTGGATCAGCTGATTCATCGTATGATTTGCTCTCGATCCGAAGTTGACTCTAAAAAAATCTCTATAGGCGA
CCTTTCCGGTCATGACTTTCAAAGAATTGTTTCGGTAATCAATGAAATGCAGGAACACACTTTGCTCATTGATGATCAGC
CAGGGTTAAAAGTTTCTGATCTACGAGCTCGGGCTCGTAGAATGAAGGAAAGCTATGATATTCAATTTCTCATTATTGAT
TATTTACAATTACTTTCCGGCTCAGGGACTTTGCGTGCTACAGAAAGTCGTCAAACAGAAATTTCAGAGATTTCCCGAAT
GTTGAAGACTCTTGCCCGTGAGTTAAACATTCCGATTCTTTGTCTTTCACAGCTTTCTCGAAAAGTTGAGGATCGGGCAA
ATCATCGTCCGATGATGAGTGATCTTCGGGAAAGTGGAAGTATTGAGCAAGATTCGGATTTAGTGATGTTCTTACTTCGT
AGAGAATATTATGATCCCAATGATAAGCCCGGCACTGCAGAACTTATTATAGCAAAAAACCGTCATGGTTCTATAGGTTC
TGTCCCTCTAGTTTTTGAAAAAGAACTCGCACGTTTTCGCAATTATTCGGCTTTTGAATGTATCAGCTAG

Upstream 100 bases:

>100_bases
AGAAGTATCCTAAACCCTCACGAATTACGAAAGTTCGCTTCTAATGGACAAATCTACTGGTGTACCTCTCCCCTCTCCTC
CTCATTCGAAAGAATCGGAA

Downstream 100 bases:

>100_bases
TTGATTCGCAATGCGAATCAGAGTCAAGTAGATTGGACAATCCGATTTGTCTTAAAACCCAAGAATCAGTATAGTTGTTT
GGTCGGCTGAGGATAGCACC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MIVLGCMLTGVHYLNLAANQLYEEDFYYLEHKIIFRVLQDAFKQDKPIDVHLAGEELKRHNQITVIGGPSYLITLAEFAG
TAAYLEEYVDIIRSKSILRKMISTAKEIEKRALEQPKNVAEALDEAQNSFFKISQSTSVSQYTLVADKLRGLTTTTDKPY
LVQLQERQELFLQNAQGDNKSFFTGIPTHFIDLDQLIHGFSPSNLMILAARPAMGKTALALNIAENLCFQNRLPIGIFSL
EMTVDQLIHRMICSRSEVDSKKISIGDLSGHDFQRIVSVINEMQEHTLLIDDQPGLKVSDLRARARRMKESYDIQFLIID
YLQLLSGSGTLRATESRQTEISEISRMLKTLARELNIPILCLSQLSRKVEDRANHRPMMSDLRESGSIEQDSDLVMFLLR
REYYDPNDKPGTAELIIAKNRHGSIGSVPLVFEKELARFRNYSAFECIS

Sequences:

>Translated_449_residues
MIVLGCMLTGVHYLNLAANQLYEEDFYYLEHKIIFRVLQDAFKQDKPIDVHLAGEELKRHNQITVIGGPSYLITLAEFAG
TAAYLEEYVDIIRSKSILRKMISTAKEIEKRALEQPKNVAEALDEAQNSFFKISQSTSVSQYTLVADKLRGLTTTTDKPY
LVQLQERQELFLQNAQGDNKSFFTGIPTHFIDLDQLIHGFSPSNLMILAARPAMGKTALALNIAENLCFQNRLPIGIFSL
EMTVDQLIHRMICSRSEVDSKKISIGDLSGHDFQRIVSVINEMQEHTLLIDDQPGLKVSDLRARARRMKESYDIQFLIID
YLQLLSGSGTLRATESRQTEISEISRMLKTLARELNIPILCLSQLSRKVEDRANHRPMMSDLRESGSIEQDSDLVMFLLR
REYYDPNDKPGTAELIIAKNRHGSIGSVPLVFEKELARFRNYSAFECIS
>Mature_449_residues
MIVLGCMLTGVHYLNLAANQLYEEDFYYLEHKIIFRVLQDAFKQDKPIDVHLAGEELKRHNQITVIGGPSYLITLAEFAG
TAAYLEEYVDIIRSKSILRKMISTAKEIEKRALEQPKNVAEALDEAQNSFFKISQSTSVSQYTLVADKLRGLTTTTDKPY
LVQLQERQELFLQNAQGDNKSFFTGIPTHFIDLDQLIHGFSPSNLMILAARPAMGKTALALNIAENLCFQNRLPIGIFSL
EMTVDQLIHRMICSRSEVDSKKISIGDLSGHDFQRIVSVINEMQEHTLLIDDQPGLKVSDLRARARRMKESYDIQFLIID
YLQLLSGSGTLRATESRQTEISEISRMLKTLARELNIPILCLSQLSRKVEDRANHRPMMSDLRESGSIEQDSDLVMFLLR
REYYDPNDKPGTAELIIAKNRHGSIGSVPLVFEKELARFRNYSAFECIS

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=446, Percent_Identity=39.0134529147982, Blast_Score=295, Evalue=6e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51043; Mature: 51043

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLGCMLTGVHYLNLAANQLYEEDFYYLEHKIIFRVLQDAFKQDKPIDVHLAGEELKRH
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHC
NQITVIGGPSYLITLAEFAGTAAYLEEYVDIIRSKSILRKMISTAKEIEKRALEQPKNVA
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
EALDEAQNSFFKISQSTSVSQYTLVADKLRGLTTTTDKPYLVQLQERQELFLQNAQGDNK
HHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCC
SFFTGIPTHFIDLDQLIHGFSPSNLMILAARPAMGKTALALNIAENLCFQNRLPIGIFSL
CEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEHH
EMTVDQLIHRMICSRSEVDSKKISIGDLSGHDFQRIVSVINEMQEHTLLIDDQPGLKVSD
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCHHH
LRARARRMKESYDIQFLIIDYLQLLSGSGTLRATESRQTEISEISRMLKTLARELNIPIL
HHHHHHHHHHHCCEEEHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEE
CLSQLSRKVEDRANHRPMMSDLRESGSIEQDSDLVMFLLRREYYDPNDKPGTAELIIAKN
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEC
RHGSIGSVPLVFEKELARFRNYSAFECIS
CCCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIVLGCMLTGVHYLNLAANQLYEEDFYYLEHKIIFRVLQDAFKQDKPIDVHLAGEELKRH
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHC
NQITVIGGPSYLITLAEFAGTAAYLEEYVDIIRSKSILRKMISTAKEIEKRALEQPKNVA
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
EALDEAQNSFFKISQSTSVSQYTLVADKLRGLTTTTDKPYLVQLQERQELFLQNAQGDNK
HHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCC
SFFTGIPTHFIDLDQLIHGFSPSNLMILAARPAMGKTALALNIAENLCFQNRLPIGIFSL
CEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEHH
EMTVDQLIHRMICSRSEVDSKKISIGDLSGHDFQRIVSVINEMQEHTLLIDDQPGLKVSD
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCHHH
LRARARRMKESYDIQFLIIDYLQLLSGSGTLRATESRQTEISEISRMLKTLARELNIPIL
HHHHHHHHHHHCCEEEHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEE
CLSQLSRKVEDRANHRPMMSDLRESGSIEQDSDLVMFLLRREYYDPNDKPGTAELIIAKN
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEC
RHGSIGSVPLVFEKELARFRNYSAFECIS
CCCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]