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The map label for this gene is prfB

Identifier: 15836107

GI number: 15836107

Start: 665812

End: 666864

Strand: Reverse

Name: prfB

Synonym: CPj0576A

Alternate gene names: 15836107

Gene position: 666864-665812 (Counterclockwise)

Preceding gene: 15836113

Following gene: 15836106

Centisome position: 54.37

GC content: 42.17

Gene sequence:

>1053_bases
CTGCGAGGTCTCTTTGACCTCGATAAAAAACAAAAAGAACTTCAAGTTTTAGAAGAAGAAAGTTCCGAAGAAAATTTTTG
GCAAGACAGTGTTCATGCTGGAAAAATTTCTGAACAGATTGTAAGTCTGAGACGACAAATCCAAGAGTATCAGGAATTAA
AAAGCAAAATAGATGCTATAGAGTTTTTCCTCGAGGATGCCGATGCTCTTGAAGACCCTGCGATTTGTGAAGACTTAGAG
AAAGAATTTCTCTTTTGTGAGAAGAAACTTGCTGTTTGGGAGACGCAACGGTTGCTTTCCGGAGAGGCAGACAAGAACTC
TTGTTTCCTTACGATCAATGCTGGGGCAGGTGGGACGGAGTCGTGTGATTGGGTAGAGATGCTGTTTCGTATGTATTCCC
GATGGGCGACGAAACATCAATGGGCCTTAGAGGTTGTCGATCGCTTAGATGGTGAAGTTGTTGGAATTAAGCATGTTACT
GTAAAGTTTTCAGGAATGTATGCTTATGGGTATGCCAAGGCAGAGCGAGGAGTACATCGTTTGGTGCGTATCTCACCTTT
CGATAGTAATGGGAAACGTCACACTAGCTTTGCTTCTGTAGACGTCTTCCCTGAGATTGATGATCAGATTAAGATTGAGA
TACGACCTAATGATTTACGTATAGATACGTTTCGTTCTTCGGGAGCAGGAGGACAACACGTCAACGTTACGGAATCCGCA
GTCAGGATCACGCACCTACCTTCTGGAGTCGTTGTTTCATGTCAAAATGAACGTAGTCAGATACAGAATCGTGAGAGCTG
TATGAAAATGCTACAAGCAAAGTTGTATCAGCAGGTTTTACAAGAACGTTTAGAGAAGCAATCTCTTGATCGCAAAGATA
AAAAGGAAATTGCTTGGGGATCTCAAATTCGCAACTACGTATTTCAGCCCTATACTCTTGTTAAAGATGTACGTACGGGA
CATGAAACAGGAAATGTCCAAGCGATGCTCGACGGCGAGCTATTGGATGAATTTATTAAAGCATATTTGGCAGAGTTTGG
AGAAGTTTCATGA

Upstream 100 bases:

>100_bases
TTTGTTTTCTAGAATCTTGATTGGAATCGTAGGTTGGTAAGAGTAATGCAGGAAAATTTAGACAAGCGTTTGGAAGCACT
TCGCACGGAAATATCTTTAG

Downstream 100 bases:

>100_bases
CAGCAGAAAAGCAAAATACAGGAATTCTAGGATTAGAAATACGGTACACTCTTCCTAGCGATGCAACGTATATGCTAAAA
TGGCTGAATGATCCTAAAAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRGLFDLDKKQKELQVLEEESSEENFWQDSVHAGKISEQIVSLRRQIQEYQELKSKIDAIEFFLEDADALEDPAICEDLE
KEFLFCEKKLAVWETQRLLSGEADKNSCFLTINAGAGGTESCDWVEMLFRMYSRWATKHQWALEVVDRLDGEVVGIKHVT
VKFSGMYAYGYAKAERGVHRLVRISPFDSNGKRHTSFASVDVFPEIDDQIKIEIRPNDLRIDTFRSSGAGGQHVNVTESA
VRITHLPSGVVVSCQNERSQIQNRESCMKMLQAKLYQQVLQERLEKQSLDRKDKKEIAWGSQIRNYVFQPYTLVKDVRTG
HETGNVQAMLDGELLDEFIKAYLAEFGEVS

Sequences:

>Translated_350_residues
MRGLFDLDKKQKELQVLEEESSEENFWQDSVHAGKISEQIVSLRRQIQEYQELKSKIDAIEFFLEDADALEDPAICEDLE
KEFLFCEKKLAVWETQRLLSGEADKNSCFLTINAGAGGTESCDWVEMLFRMYSRWATKHQWALEVVDRLDGEVVGIKHVT
VKFSGMYAYGYAKAERGVHRLVRISPFDSNGKRHTSFASVDVFPEIDDQIKIEIRPNDLRIDTFRSSGAGGQHVNVTESA
VRITHLPSGVVVSCQNERSQIQNRESCMKMLQAKLYQQVLQERLEKQSLDRKDKKEIAWGSQIRNYVFQPYTLVKDVRTG
HETGNVQAMLDGELLDEFIKAYLAEFGEVS
>Mature_350_residues
MRGLFDLDKKQKELQVLEEESSEENFWQDSVHAGKISEQIVSLRRQIQEYQELKSKIDAIEFFLEDADALEDPAICEDLE
KEFLFCEKKLAVWETQRLLSGEADKNSCFLTINAGAGGTESCDWVEMLFRMYSRWATKHQWALEVVDRLDGEVVGIKHVT
VKFSGMYAYGYAKAERGVHRLVRISPFDSNGKRHTSFASVDVFPEIDDQIKIEIRPNDLRIDTFRSSGAGGQHVNVTESA
VRITHLPSGVVVSCQNERSQIQNRESCMKMLQAKLYQQVLQERLEKQSLDRKDKKEIAWGSQIRNYVFQPYTLVKDVRTG
HETGNVQAMLDGELLDEFIKAYLAEFGEVS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=279, Percent_Identity=38.7096774193548, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI34577120, Length=307, Percent_Identity=34.2019543973941, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI166795305, Length=173, Percent_Identity=39.8843930635838, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI2367172, Length=344, Percent_Identity=47.9651162790698, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI1787462, Length=245, Percent_Identity=40.4081632653061, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=272, Percent_Identity=30.1470588235294, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=335, Percent_Identity=31.6417910447761, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19921226, Length=223, Percent_Identity=39.0134529147982, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40161; Mature: 40161

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLFDLDKKQKELQVLEEESSEENFWQDSVHAGKISEQIVSLRRQIQEYQELKSKIDAI
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFFLEDADALEDPAICEDLEKEFLFCEKKLAVWETQRLLSGEADKNSCFLTINAGAGGTE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
SCDWVEMLFRMYSRWATKHQWALEVVDRLDGEVVGIKHVTVKFSGMYAYGYAKAERGVHR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECEEEEHHHHHHHHHHH
LVRISPFDSNGKRHTSFASVDVFPEIDDQIKIEIRPNDLRIDTFRSSGAGGQHVNVTESA
HEEECCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEHHHH
VRITHLPSGVVVSCQNERSQIQNRESCMKMLQAKLYQQVLQERLEKQSLDRKDKKEIAWG
EEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SQIRNYVFQPYTLVKDVRTGHETGNVQAMLDGELLDEFIKAYLAEFGEVS
HHHHHHHCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGLFDLDKKQKELQVLEEESSEENFWQDSVHAGKISEQIVSLRRQIQEYQELKSKIDAI
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFFLEDADALEDPAICEDLEKEFLFCEKKLAVWETQRLLSGEADKNSCFLTINAGAGGTE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
SCDWVEMLFRMYSRWATKHQWALEVVDRLDGEVVGIKHVTVKFSGMYAYGYAKAERGVHR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECEEEEHHHHHHHHHHH
LVRISPFDSNGKRHTSFASVDVFPEIDDQIKIEIRPNDLRIDTFRSSGAGGQHVNVTESA
HEEECCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEHHHH
VRITHLPSGVVVSCQNERSQIQNRESCMKMLQAKLYQQVLQERLEKQSLDRKDKKEIAWG
EEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SQIRNYVFQPYTLVKDVRTGHETGNVQAMLDGELLDEFIKAYLAEFGEVS
HHHHHHHCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]