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The map label for this gene is dnaA

Identifier: 15835957

GI number: 15835957

Start: 469322

End: 470674

Strand: Direct

Name: dnaA

Synonym: CPj0424

Alternate gene names: 15835957

Gene position: 469322-470674 (Clockwise)

Preceding gene: 15835956

Following gene: 15835958

Centisome position: 38.26

GC content: 37.77

Gene sequence:

>1353_bases
ATGTTAACCTGTAACGAGTGCACTACTTGGGAACAGTTTTTAAATTATGTTAAGACACGTTGCTCGAAAACGGCTTTTGA
AAATTGGATTTCTCCTATTCAAGTTCTTGAAGAAACTCAAGAGAAAATTCGCTTAGAAGTCCCCAACATTTTTGTACAAA
ATTATCTTCTTGATAACTACAAAAGAGACCTCTGTTCTTTTGTCCCCTTAGATGTTCATGGAGAGCCTGCTTTAGAATTT
GTAGTTGCAGAACACAAGAAACCTTCAGCCCCCGTGGCTTCTCAAAAAGAATCAAACGAAGGAATTTCTGAGGTCTTTGA
AGAAACTAAAGATTTTGAATTAAAGCTGAATCTCTCCTATCGCTTTGATAATTTCATTGAAGGTCCCTCAAATCAATTTG
TGAAGTCTGCAGCTGTAGGTATTGCTGGGAAACCTGGCCGCTCCTACAACCCTTTATTCATCCATGGGGGTGTGGGATTA
GGCAAAACGCATTTACTTCATGCCGTAGGTCACTACGTAAGAGAACATCATAAAAATCTACGCATCCATTGCATCACTAC
AGAAGCGTTTATCAACGATCTTGTCTACCATCTCAAATCCAAGTCTGTTGATAAAATGAAAAATTTTTATCGTTCCCTAG
ATTTACTTCTTGTTGATGATATTCAATTTTTACAGAATCGCCAAAATTTTGAAGAAGAGTTTTGCAATACCTTTGAGACT
TTGATCAACCTGAGTAAGCAAATTGTAATTACCAGTGATAAACCTCCAAGTCAGCTCAAACTTTCCGAGCGTATCATTGC
TAGAATGGAATGGGGACTGGTTGCTCACGTCGGCATCCCTGATTTAGAAACTCGGGTTGCGATTTTACAGCACAAGGCGG
AGCAAAAAGGATTGCTCATTCCTAATGAAATGGCATTTTATATTGCGGATCACATCTATGGCAATGTCCGTCAATTGGAA
GGAGCTATCAACAAGCTGACTGCCTATTGTCGTCTTTTCGGCAAGTCTCTTACAGAAACTACAGTCCGAGAAACTCTAAA
AGAGCTCTTCCGTTCTCCAACAAAACAAAAAATTTCTGTAGAAACGATCTTAAAAAGTGTTGCTACAGTATTCCAAGTAA
AGCTGAATGATCTTAAGGGAAACTCACGCTCTAAAGATCTTGTGTTAGCTCGGCAAATTGCTATGTATTTAGCAAAAACT
CTTATTACAGATTCTTTAGTTGCAATAGGAGCTGCTTTTGGTAAAACTCATTCGACAGTACTTTATGCCTGTAAAACTAT
AGAACATAAATTACAAAATGACGAAACTCTTAAGCGTCAAGTAAATCTCTGTAAAAATCATATTGTTGGTTAA

Upstream 100 bases:

>100_bases
TCAGTGCAATGAATAGTCAAGATGCAATGAGATTTTCTCGCCATCACTCATGGGATGGGTCGATTGTATTTGGGAAAGCT
TTATAAATAAAGAGAGCGGC

Downstream 100 bases:

>100_bases
TTTAGGGGGTGTCCCATGTTCCGTAGAACAGGAAAAGGTCCTTTTGAAGATGTGCAAACACTTTACGAAGAAGAAACTTC
TTCACCTTCCAGCTACTCGC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLTCNECTTWEQFLNYVKTRCSKTAFENWISPIQVLEETQEKIRLEVPNIFVQNYLLDNYKRDLCSFVPLDVHGEPALEF
VVAEHKKPSAPVASQKESNEGISEVFEETKDFELKLNLSYRFDNFIEGPSNQFVKSAAVGIAGKPGRSYNPLFIHGGVGL
GKTHLLHAVGHYVREHHKNLRIHCITTEAFINDLVYHLKSKSVDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
LINLSKQIVITSDKPPSQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLLIPNEMAFYIADHIYGNVRQLE
GAINKLTAYCRLFGKSLTETTVRETLKELFRSPTKQKISVETILKSVATVFQVKLNDLKGNSRSKDLVLARQIAMYLAKT
LITDSLVAIGAAFGKTHSTVLYACKTIEHKLQNDETLKRQVNLCKNHIVG

Sequences:

>Translated_450_residues
MLTCNECTTWEQFLNYVKTRCSKTAFENWISPIQVLEETQEKIRLEVPNIFVQNYLLDNYKRDLCSFVPLDVHGEPALEF
VVAEHKKPSAPVASQKESNEGISEVFEETKDFELKLNLSYRFDNFIEGPSNQFVKSAAVGIAGKPGRSYNPLFIHGGVGL
GKTHLLHAVGHYVREHHKNLRIHCITTEAFINDLVYHLKSKSVDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
LINLSKQIVITSDKPPSQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLLIPNEMAFYIADHIYGNVRQLE
GAINKLTAYCRLFGKSLTETTVRETLKELFRSPTKQKISVETILKSVATVFQVKLNDLKGNSRSKDLVLARQIAMYLAKT
LITDSLVAIGAAFGKTHSTVLYACKTIEHKLQNDETLKRQVNLCKNHIVG
>Mature_450_residues
MLTCNECTTWEQFLNYVKTRCSKTAFENWISPIQVLEETQEKIRLEVPNIFVQNYLLDNYKRDLCSFVPLDVHGEPALEF
VVAEHKKPSAPVASQKESNEGISEVFEETKDFELKLNLSYRFDNFIEGPSNQFVKSAAVGIAGKPGRSYNPLFIHGGVGL
GKTHLLHAVGHYVREHHKNLRIHCITTEAFINDLVYHLKSKSVDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
LINLSKQIVITSDKPPSQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLLIPNEMAFYIADHIYGNVRQLE
GAINKLTAYCRLFGKSLTETTVRETLKELFRSPTKQKISVETILKSVATVFQVKLNDLKGNSRSKDLVLARQIAMYLAKT
LITDSLVAIGAAFGKTHSTVLYACKTIEHKLQNDETLKRQVNLCKNHIVG

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=448, Percent_Identity=36.6071428571429, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA2_CHLPN (Q9Z8B9)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A81590
- PIR:   B72081
- PIR:   F86543
- RefSeq:   NP_224624.1
- RefSeq:   NP_300481.1
- RefSeq:   NP_444878.1
- RefSeq:   NP_876714.1
- ProteinModelPortal:   Q9Z8B9
- GeneID:   1467121
- GeneID:   895183
- GeneID:   919867
- GeneID:   963801
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0329
- KEGG:   cpn:CPn0424
- KEGG:   cpt:CpB0440
- TIGR:   CP_0329
- HOGENOM:   HBG695666
- OMA:   IQRVVGQ
- PhylomeDB:   Q9Z8B9
- ProtClustDB:   PRK00149
- BioCyc:   CPNE115711:CP_0329-MONOMER
- BioCyc:   CPNE115713:CPN0424-MONOMER
- BioCyc:   CPNE138677:CPJ0424-MONOMER
- BioCyc:   CPNE182082:CPB0440-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 51240; Mature: 51240

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTCNECTTWEQFLNYVKTRCSKTAFENWISPIQVLEETQEKIRLEVPNIFVQNYLLDNY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KRDLCSFVPLDVHGEPALEFVVAEHKKPSAPVASQKESNEGISEVFEETKDFELKLNLSY
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEEE
RFDNFIEGPSNQFVKSAAVGIAGKPGRSYNPLFIHGGVGLGKTHLLHAVGHYVREHHKNL
CCHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
RIHCITTEAFINDLVYHLKSKSVDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
EEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LINLSKQIVITSDKPPSQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLLI
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
PNEMAFYIADHIYGNVRQLEGAINKLTAYCRLFGKSLTETTVRETLKELFRSPTKQKISV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ETILKSVATVFQVKLNDLKGNSRSKDLVLARQIAMYLAKTLITDSLVAIGAAFGKTHSTV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LYACKTIEHKLQNDETLKRQVNLCKNHIVG
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTCNECTTWEQFLNYVKTRCSKTAFENWISPIQVLEETQEKIRLEVPNIFVQNYLLDNY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KRDLCSFVPLDVHGEPALEFVVAEHKKPSAPVASQKESNEGISEVFEETKDFELKLNLSY
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEEE
RFDNFIEGPSNQFVKSAAVGIAGKPGRSYNPLFIHGGVGLGKTHLLHAVGHYVREHHKNL
CCHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
RIHCITTEAFINDLVYHLKSKSVDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
EEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LINLSKQIVITSDKPPSQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLLI
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
PNEMAFYIADHIYGNVRQLEGAINKLTAYCRLFGKSLTETTVRETLKELFRSPTKQKISV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ETILKSVATVFQVKLNDLKGNSRSKDLVLARQIAMYLAKTLITDSLVAIGAAFGKTHSTV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LYACKTIEHKLQNDETLKRQVNLCKNHIVG
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362