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The map label for this gene is mraW

Identifier: 15835954

GI number: 15835954

Start: 466815

End: 467708

Strand: Reverse

Name: mraW

Synonym: CPj0421

Alternate gene names: 15835954

Gene position: 467708-466815 (Counterclockwise)

Preceding gene: 15835959

Following gene: 15835953

Centisome position: 38.13

GC content: 42.73

Gene sequence:

>894_bases
ATGTCCGAACGTGCGCATATTCCCGTATTAGTTGAAGAATGTTTAGCTTTATTTGCTCAACGTCCTCCACAGACTTTTCG
AGATGTCACCTTAGGAGCTGGAGGACATGCGTATGCTTTTCTTGAGGCGTATCCCTCTCTAACTTGTTATGATGGCTCCG
ATCGAGATCTTCAGGCTTTGGCAATTGCAGAAAAACGTTTGGAGACCTTTCAAGATAGAGTCTCCTTTTCCCACGCCTCT
TTTGAAGATCTTGCGAACCAACCCACTCCACGTCTTTATGACGGAGTTCTTGCAGATTTAGGAGTCTCTTCTATGCAGCT
GGATACTCTATCCCGAGGGTTTAGCTTTCAAGGGGAAAAAGAAGAGTTGGATATGCGTATGGATCAAACGCAAGAGCTTT
CCGCTAGCGATGTCCTGAACTCCCTAAAAGAAGAAGAACTAGGGAGAATTTTTCGTGAATATGGAGAGGAACCACAATGG
AAATCTGCAGCTAAAGCTGTTGTCCATTTTCGTAAGCATAAAAAAATTCTTTCGATCCAGGATGTAAAAGAAGCTCTTCT
TGGCGTTTTCCCTCACTATCGTTTTCATAGAAAAATACATCCACTCACCTTGATTTTTCAAGCTCTACGTGTTTATGTGA
ATGGAGAGGATAGACAATTGAAAAGTTTACTAACATCTGCTATATCTTGGCTGGCTCCTCAGGGACGGCTTGTCATTATT
TCTTTTTGTAGCTCTGAGGATCGTCCTGTGAAGTGGTTTTTTAAAGAGGCGGAAGCTTCTGGCCTGGGGAAGGTAATCAC
AAAGAAAGTGATCCAACCTACCTACCAAGAAGTACGAAGAAATCCTAGATCGAGATCAGCAAAACTACGGTGTTTTGAAA
AAGCTTCCCAATGA

Upstream 100 bases:

>100_bases
TGGCGAACTAGCGAGAATTTAAGATAAGGGAAGGCTTTCTTCTTTCTATGGAATCCTGTATCTTTGTCCTTATTTGAAGC
CAAGAGCTTAGGAAATTCTT

Downstream 100 bases:

>100_bases
ACAAAAGTCGTTTTTTACGTTTATGCTGCTGTCTATGCTTTTGTGGAAGTCTCTTTTATTTCTATATTAATAAGCAGAAC
TCGCTGACGAAATTACGCCT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSERAHIPVLVEECLALFAQRPPQTFRDVTLGAGGHAYAFLEAYPSLTCYDGSDRDLQALAIAEKRLETFQDRVSFSHAS
FEDLANQPTPRLYDGVLADLGVSSMQLDTLSRGFSFQGEKEELDMRMDQTQELSASDVLNSLKEEELGRIFREYGEEPQW
KSAAKAVVHFRKHKKILSIQDVKEALLGVFPHYRFHRKIHPLTLIFQALRVYVNGEDRQLKSLLTSAISWLAPQGRLVII
SFCSSEDRPVKWFFKEAEASGLGKVITKKVIQPTYQEVRRNPRSRSAKLRCFEKASQ

Sequences:

>Translated_297_residues
MSERAHIPVLVEECLALFAQRPPQTFRDVTLGAGGHAYAFLEAYPSLTCYDGSDRDLQALAIAEKRLETFQDRVSFSHAS
FEDLANQPTPRLYDGVLADLGVSSMQLDTLSRGFSFQGEKEELDMRMDQTQELSASDVLNSLKEEELGRIFREYGEEPQW
KSAAKAVVHFRKHKKILSIQDVKEALLGVFPHYRFHRKIHPLTLIFQALRVYVNGEDRQLKSLLTSAISWLAPQGRLVII
SFCSSEDRPVKWFFKEAEASGLGKVITKKVIQPTYQEVRRNPRSRSAKLRCFEKASQ
>Mature_296_residues
SERAHIPVLVEECLALFAQRPPQTFRDVTLGAGGHAYAFLEAYPSLTCYDGSDRDLQALAIAEKRLETFQDRVSFSHASF
EDLANQPTPRLYDGVLADLGVSSMQLDTLSRGFSFQGEKEELDMRMDQTQELSASDVLNSLKEEELGRIFREYGEEPQWK
SAAKAVVHFRKHKKILSIQDVKEALLGVFPHYRFHRKIHPLTLIFQALRVYVNGEDRQLKSLLTSAISWLAPQGRLVIIS
FCSSEDRPVKWFFKEAEASGLGKVITKKVIQPTYQEVRRNPRSRSAKLRCFEKASQ

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=36.6071428571429, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=38.5026737967914, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI1786270, Length=325, Percent_Identity=34.4615384615385, Blast_Score=151, Evalue=5e-38,
Organism=Drosophila melanogaster, GI62472493, Length=337, Percent_Identity=33.5311572700297, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI28571637, Length=337, Percent_Identity=33.5311572700297, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CHLPN (Q9Z8C2)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86543
- PIR:   G72080
- RefSeq:   NP_224621.1
- RefSeq:   NP_300478.1
- RefSeq:   NP_444882.1
- RefSeq:   NP_876711.1
- ProteinModelPortal:   Q9Z8C2
- GeneID:   1467118
- GeneID:   895082
- GeneID:   919158
- GeneID:   962872
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0333
- KEGG:   cpn:CPn0421
- KEGG:   cpt:CpB0437
- TIGR:   CP_0333
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   CPNE115711:CP_0333-MONOMER
- BioCyc:   CPNE115713:CPN0421-MONOMER
- BioCyc:   CPNE138677:CPJ0421-MONOMER
- BioCyc:   CPNE182082:CPB0437-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33839; Mature: 33708

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 81-81 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERAHIPVLVEECLALFAQRPPQTFRDVTLGAGGHAYAFLEAYPSLTCYDGSDRDLQAL
CCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCHHHH
AIAEKRLETFQDRVSFSHASFEDLANQPTPRLYDGVLADLGVSSMQLDTLSRGFSFQGEK
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCH
EELDMRMDQTQELSASDVLNSLKEEELGRIFREYGEEPQWKSAAKAVVHFRKHKKILSIQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DVKEALLGVFPHYRFHRKIHPLTLIFQALRVYVNGEDRQLKSLLTSAISWLAPQGRLVII
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEE
SFCSSEDRPVKWFFKEAEASGLGKVITKKVIQPTYQEVRRNPRSRSAKLRCFEKASQ
EECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SERAHIPVLVEECLALFAQRPPQTFRDVTLGAGGHAYAFLEAYPSLTCYDGSDRDLQAL
CCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCHHHH
AIAEKRLETFQDRVSFSHASFEDLANQPTPRLYDGVLADLGVSSMQLDTLSRGFSFQGEK
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCH
EELDMRMDQTQELSASDVLNSLKEEELGRIFREYGEEPQWKSAAKAVVHFRKHKKILSIQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DVKEALLGVFPHYRFHRKIHPLTLIFQALRVYVNGEDRQLKSLLTSAISWLAPQGRLVII
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEE
SFCSSEDRPVKWFFKEAEASGLGKVITKKVIQPTYQEVRRNPRSRSAKLRCFEKASQ
EECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362