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The map label for this gene is yhfO

Identifier: 15835929

GI number: 15835929

Start: 442947

End: 444062

Strand: Reverse

Name: yhfO

Synonym: CPj0396

Alternate gene names: 15835929

Gene position: 444062-442947 (Counterclockwise)

Preceding gene: 15835930

Following gene: 15835924

Centisome position: 36.2

GC content: 44.62

Gene sequence:

>1116_bases
ATGATCTATTTGGATAACAATGCGATGACACCCCCAGAGAGGGGACTTTTGGAATTTCTCCAAAAAACCTTCCTTATAGA
AGGGACGTACGCGAATCCTTCGAGCGTCCATCAATTAGGTAAAAAATCTCGTCAACTGGTTCTAGAAGCTTCACACTGGA
TGCAAAAGGTCCTTTCGTTTCAGGGCCGTGTCCTCTATACCTCAGGGGCTACTGAGAGTTTAAATTTAGCAATAGCAAGC
CTCCCTAAAGACAGTCATGTTATCACCTCAGGTAGCGAACACCCCGCCATCTTAGAGCCTTTAAAACATTCCTCGCTTTC
CGTTTCTTATTTAAATCCCGAAGAAGGGAGATGTGTTCTTACTATAGAGCAGATTGAAAGAGCTGTGACTCCTAAAACTT
CAGCAATCATCTTAGGTTGGGTCAATAGTGAGACTGGTGCCAAAGCTGATATAGCTGCTATAGCCCACTTCGCGCAAGAA
CGACAATTGCAATTTATTGTGGATGCGACTGCAAATGTAGGTAAGGAGAGGATAGTTCTTCCCTCTGGTGTCACTATGGC
AGCATTCAGTGGACATAAATTTCATGCACTCTCTGGAATCGGAGCTCTTCTGGTCTCTCCAGGAGTCAAACTACATCCTC
AGCTGTGGGGAGGAGGTCAGCAAGGAGGGCTGCGCGCAGGCACAGAAAATCTTTGGGGAATCGCCTCTCTGCTTTATATT
TTCAAATACCTAGATCTTCATCAAGAGCGTATCTCTCAGGAAATTCTTACCCATAGAAATGGTTTTGAAAAGGCAATCAA
AGCACGCATTCCTGATGTCCATATTCATTGTGCGGATCAACCACGGGCAAACAACGTCTCAGCAATTGCTTTCCCTCCGT
TGGAAGGTGAGGTATTGCAAATCGCCTTAGATATAGAAGGAGTGGCTTGTGGTTATGGATCCGCATGCTCTTCAGGTGCT
ACCGCACCCTTTAAATCTCTTGTCAGCATGGGTGTTGATGAAGAGTTGACCCTGGCAACACTCAGGTTTTCTTTTAGCCA
TCTTCTCTTGCAAGAAGATGTTGAAAGAGCCGTTGGAATTATAGAAAAAGTCGTAGAACGTTTGAAAAATTCCTAA

Upstream 100 bases:

>100_bases
GAATGCATTAATTTCTCTAGCCAATACTCGTGGAGGCGATGACAACGCTACTGTCGTATTAGTCCGAATACAATAGTTCC
TTTGCTAAGGATAGTATTCC

Downstream 100 bases:

>100_bases
GTCTTAAAAGAGAACATGTTTCTAAGCTGAAAGAACACTCCTGACTCTTATTGCAGAATCTATGAGAGTAAGTTTTTAAT
CGATACGGTTTTTATCCCAG

Product: NifS-related aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIYLDNNAMTPPERGLLEFLQKTFLIEGTYANPSSVHQLGKKSRQLVLEASHWMQKVLSFQGRVLYTSGATESLNLAIAS
LPKDSHVITSGSEHPAILEPLKHSSLSVSYLNPEEGRCVLTIEQIERAVTPKTSAIILGWVNSETGAKADIAAIAHFAQE
RQLQFIVDATANVGKERIVLPSGVTMAAFSGHKFHALSGIGALLVSPGVKLHPQLWGGGQQGGLRAGTENLWGIASLLYI
FKYLDLHQERISQEILTHRNGFEKAIKARIPDVHIHCADQPRANNVSAIAFPPLEGEVLQIALDIEGVACGYGSACSSGA
TAPFKSLVSMGVDEELTLATLRFSFSHLLLQEDVERAVGIIEKVVERLKNS

Sequences:

>Translated_371_residues
MIYLDNNAMTPPERGLLEFLQKTFLIEGTYANPSSVHQLGKKSRQLVLEASHWMQKVLSFQGRVLYTSGATESLNLAIAS
LPKDSHVITSGSEHPAILEPLKHSSLSVSYLNPEEGRCVLTIEQIERAVTPKTSAIILGWVNSETGAKADIAAIAHFAQE
RQLQFIVDATANVGKERIVLPSGVTMAAFSGHKFHALSGIGALLVSPGVKLHPQLWGGGQQGGLRAGTENLWGIASLLYI
FKYLDLHQERISQEILTHRNGFEKAIKARIPDVHIHCADQPRANNVSAIAFPPLEGEVLQIALDIEGVACGYGSACSSGA
TAPFKSLVSMGVDEELTLATLRFSFSHLLLQEDVERAVGIIEKVVERLKNS
>Mature_371_residues
MIYLDNNAMTPPERGLLEFLQKTFLIEGTYANPSSVHQLGKKSRQLVLEASHWMQKVLSFQGRVLYTSGATESLNLAIAS
LPKDSHVITSGSEHPAILEPLKHSSLSVSYLNPEEGRCVLTIEQIERAVTPKTSAIILGWVNSETGAKADIAAIAHFAQE
RQLQFIVDATANVGKERIVLPSGVTMAAFSGHKFHALSGIGALLVSPGVKLHPQLWGGGQQGGLRAGTENLWGIASLLYI
FKYLDLHQERISQEILTHRNGFEKAIKARIPDVHIHCADQPRANNVSAIAFPPLEGEVLQIALDIEGVACGYGSACSSGA
TAPFKSLVSMGVDEELTLATLRFSFSHLLLQEDVERAVGIIEKVVERLKNS

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=29.6103896103896, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=26.7942583732057, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI48994898, Length=382, Percent_Identity=31.6753926701571, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25143064, Length=391, Percent_Identity=29.4117647058824, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17533177, Length=315, Percent_Identity=27.9365079365079, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319831, Length=390, Percent_Identity=29.2307692307692, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI20129463, Length=397, Percent_Identity=29.9748110831234, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40243; Mature: 40243

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNNAMTPPERGLLEFLQKTFLIEGTYANPSSVHQLGKKSRQLVLEASHWMQKVLSF
CEEECCCCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGRVLYTSGATESLNLAIASLPKDSHVITSGSEHPAILEPLKHSSLSVSYLNPEEGRCVL
CCEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCEEE
TIEQIERAVTPKTSAIILGWVNSETGAKADIAAIAHFAQERQLQFIVDATANVGKERIVL
EHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEE
PSGVTMAAFSGHKFHALSGIGALLVSPGVKLHPQLWGGGQQGGLRAGTENLWGIASLLYI
CCCCEEEEECCCCEEHHHCCHHHHHCCCCEECHHCCCCCCCCCCCCCCHHHHHHHHHHHH
FKYLDLHQERISQEILTHRNGFEKAIKARIPDVHIHCADQPRANNVSAIAFPPLEGEVLQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCEEE
IALDIEGVACGYGSACSSGATAPFKSLVSMGVDEELTLATLRFSFSHLLLQEDVERAVGI
EEEEECEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IEKVVERLKNS
HHHHHHHHHCC
>Mature Secondary Structure
MIYLDNNAMTPPERGLLEFLQKTFLIEGTYANPSSVHQLGKKSRQLVLEASHWMQKVLSF
CEEECCCCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGRVLYTSGATESLNLAIASLPKDSHVITSGSEHPAILEPLKHSSLSVSYLNPEEGRCVL
CCEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCEEE
TIEQIERAVTPKTSAIILGWVNSETGAKADIAAIAHFAQERQLQFIVDATANVGKERIVL
EHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEE
PSGVTMAAFSGHKFHALSGIGALLVSPGVKLHPQLWGGGQQGGLRAGTENLWGIASLLYI
CCCCEEEEECCCCEEHHHCCHHHHHCCCCEECHHCCCCCCCCCCCCCCHHHHHHHHHHHH
FKYLDLHQERISQEILTHRNGFEKAIKARIPDVHIHCADQPRANNVSAIAFPPLEGEVLQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCEEE
IALDIEGVACGYGSACSSGATAPFKSLVSMGVDEELTLATLRFSFSHLLLQEDVERAVGI
EEEEECEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IEKVVERLKNS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]