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The map label for this gene is ychF

Identifier: 15835856

GI number: 15835856

Start: 364386

End: 365480

Strand: Reverse

Name: ychF

Synonym: CPj0321

Alternate gene names: 15835856

Gene position: 365480-364386 (Counterclockwise)

Preceding gene: 15835865

Following gene: 15835849

Centisome position: 29.8

GC content: 40.0

Gene sequence:

>1095_bases
ATGAGTCATACTGAATGTGGAATTGTAGGGCTTCCTAATGTAGGAAAGTCTGGCTTATTCAATGCTTTAACAGGAGCTCA
AGTTGCCTCCTGTAACTATCCGTTTTGTACTATCGATCCTAATGTGGGTATTGTTCCTGTTATCGATGAAAGACTGGAAG
CCTTAGCTAAAATTAGCAATAGTCAGAAGATCATCTATGCGGATATGAAATTTGTAGATATTGCAGGTTTAGTTAAGGGA
GCTTCCGATGGCGCGGGTCTGGGAAATCGGTTTCTCTCTCATATTCGAGAAACTCATGCTATTGCTCATGTAGTGCGTTG
TTTTGATGATCCAGACGTTACACACGTTTCAGGAAAAGTCAACCCTGTTGAGGATATTGAAGTTATCAACTTAGAGCTCA
TTTTTTCTGACTTCTCCTCAGCAAAAAATATCCATAGCAAATTAGAAAAGCTAGCCAAAGGAAAGCGTGAAGTAGGAGCT
CTCTTGCCTCTATTTGATACAATTATTGCTCACTTAGAAAAGGGGCTGCCGCTACGTACTTTAGAATTAACTCCAGAACA
AATTGTGGCATTAAAGCCCTATCCGTTTTTGACCATGAAGCCTATGTTTTATATAGCTAATGTTGACGAGAGTTCTCTAC
CAGATATGGATAATGATTATGTTGCCGCTGTTCGGGAAGTTGCTGCAAAAGAAAATTCTAAAGTGGTTCCTATCTGTGTT
CGTATAGAAGAAGAAATCGTTTCCTTACCTATTGAAGAGCGCTTAGAATTTCTTATGAGCTTAGGTCTTGAAAAATCAGG
ACTTCATAGATTAGTGCGTGCTGCGTATGACACTTTAGGACTGATTTCTTATTTTACTACAGGTCCTCAAGAATCTCGTG
CATGGACAGTGGTTCGAGGGTCTTCTGCTTGGGAAGCTGCTGGAGAAATCCATACGGATATTCAAAAGGGCTTTATTCGT
GCTGAAGTGATTACTTTTGAAGATATGATAGAGTGTCAAGGTCGTGCAGCTGCTCGAGAATTAGGGAAATTACATATAGA
AGGACGTGATTATATCGTCCAGGACGGTGATACTATGCTGTTCCTTCATAATTAA

Upstream 100 bases:

>100_bases
GAAGGTACTATTTTTCTAGCTATTGTGCTTTTTTGAAACAGAAGAGTTGTCTTTCTAATATTCGAAAAAGCATGTCATTA
TTTTTATTTTTAGATGTCTT

Downstream 100 bases:

>100_bases
AGGAACCCTTTGCAAACCAATCTTGAGCATCCAAAATGTCTTTTTCAATTGCTCGTATTAGAGTTTCTTTTGATTGAAAC
TTTTTTTCTTCTCTAAGAAA

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNVGIVPVIDERLEALAKISNSQKIIYADMKFVDIAGLVKG
ASDGAGLGNRFLSHIRETHAIAHVVRCFDDPDVTHVSGKVNPVEDIEVINLELIFSDFSSAKNIHSKLEKLAKGKREVGA
LLPLFDTIIAHLEKGLPLRTLELTPEQIVALKPYPFLTMKPMFYIANVDESSLPDMDNDYVAAVREVAAKENSKVVPICV
RIEEEIVSLPIEERLEFLMSLGLEKSGLHRLVRAAYDTLGLISYFTTGPQESRAWTVVRGSSAWEAAGEIHTDIQKGFIR
AEVITFEDMIECQGRAAARELGKLHIEGRDYIVQDGDTMLFLHN

Sequences:

>Translated_364_residues
MSHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNVGIVPVIDERLEALAKISNSQKIIYADMKFVDIAGLVKG
ASDGAGLGNRFLSHIRETHAIAHVVRCFDDPDVTHVSGKVNPVEDIEVINLELIFSDFSSAKNIHSKLEKLAKGKREVGA
LLPLFDTIIAHLEKGLPLRTLELTPEQIVALKPYPFLTMKPMFYIANVDESSLPDMDNDYVAAVREVAAKENSKVVPICV
RIEEEIVSLPIEERLEFLMSLGLEKSGLHRLVRAAYDTLGLISYFTTGPQESRAWTVVRGSSAWEAAGEIHTDIQKGFIR
AEVITFEDMIECQGRAAARELGKLHIEGRDYIVQDGDTMLFLHN
>Mature_363_residues
SHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNVGIVPVIDERLEALAKISNSQKIIYADMKFVDIAGLVKGA
SDGAGLGNRFLSHIRETHAIAHVVRCFDDPDVTHVSGKVNPVEDIEVINLELIFSDFSSAKNIHSKLEKLAKGKREVGAL
LPLFDTIIAHLEKGLPLRTLELTPEQIVALKPYPFLTMKPMFYIANVDESSLPDMDNDYVAAVREVAAKENSKVVPICVR
IEEEIVSLPIEERLEFLMSLGLEKSGLHRLVRAAYDTLGLISYFTTGPQESRAWTVVRGSSAWEAAGEIHTDIQKGFIRA
EVITFEDMIECQGRAAARELGKLHIEGRDYIVQDGDTMLFLHN

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=368, Percent_Identity=41.5760869565217, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI58761502, Length=196, Percent_Identity=35.7142857142857, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1787454, Length=361, Percent_Identity=53.185595567867, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1789574, Length=129, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=368, Percent_Identity=38.8586956521739, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=38.1081081081081, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321773, Length=378, Percent_Identity=32.2751322751323, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6321649, Length=334, Percent_Identity=29.0419161676647, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640873, Length=371, Percent_Identity=40.4312668463612, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24640877, Length=371, Percent_Identity=40.4312668463612, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24640875, Length=371, Percent_Identity=40.4312668463612, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=39.0243902439024, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40059; Mature: 39927

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNVGIVPVIDERLEALAKISN
CCCCCCCEEECCCCCCCHHHHHHCCCEEEECCCCEEEECCCCCEEECHHHHHHHHHHHCC
SQKIIYADMKFVDIAGLVKGASDGAGLGNRFLSHIRETHAIAHVVRCFDDPDVTHVSGKV
CCEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
NPVEDIEVINLELIFSDFSSAKNIHSKLEKLAKGKREVGALLPLFDTIIAHLEKGLPLRT
CCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LELTPEQIVALKPYPFLTMKPMFYIANVDESSLPDMDNDYVAAVREVAAKENSKVVPICV
EECCHHHEEEECCCCCEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEE
RIEEEIVSLPIEERLEFLMSLGLEKSGLHRLVRAAYDTLGLISYFTTGPQESRAWTVVRG
EEHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
SSAWEAAGEIHTDIQKGFIRAEVITFEDMIECQGRAAARELGKLHIEGRDYIVQDGDTML
CCCHHHHHHHHHHHHHCHHEEEEEEHHHHHHHCCHHHHHHHCEEEECCCEEEEECCCEEE
FLHN
EEEC
>Mature Secondary Structure 
SHTECGIVGLPNVGKSGLFNALTGAQVASCNYPFCTIDPNVGIVPVIDERLEALAKISN
CCCCCCEEECCCCCCCHHHHHHCCCEEEECCCCEEEECCCCCEEECHHHHHHHHHHHCC
SQKIIYADMKFVDIAGLVKGASDGAGLGNRFLSHIRETHAIAHVVRCFDDPDVTHVSGKV
CCEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
NPVEDIEVINLELIFSDFSSAKNIHSKLEKLAKGKREVGALLPLFDTIIAHLEKGLPLRT
CCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LELTPEQIVALKPYPFLTMKPMFYIANVDESSLPDMDNDYVAAVREVAAKENSKVVPICV
EECCHHHEEEECCCCCEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEE
RIEEEIVSLPIEERLEFLMSLGLEKSGLHRLVRAAYDTLGLISYFTTGPQESRAWTVVRG
EEHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
SSAWEAAGEIHTDIQKGFIRAEVITFEDMIECQGRAAARELGKLHIEGRDYIVQDGDTML
CCCHHHHHHHHHHHHHCHHEEEEEEHHHHHHHCCHHHHHHHCEEEECCCEEEEECCCEEE
FLHN
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]