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The map label for this gene is truB

Identifier: 15835854

GI number: 15835854

Start: 362797

End: 363504

Strand: Direct

Name: truB

Synonym: CPj0319

Alternate gene names: 15835854

Gene position: 362797-363504 (Clockwise)

Preceding gene: 15835853

Following gene: 15835855

Centisome position: 29.58

GC content: 41.1

Gene sequence:

>708_bases
ATGGATCTTGCAGTAGAATTAAAAGAGGGCATTCTTCTTGTAGACAAGCCTCAAGGGAGAACTTCGTTTAGCCTTATCCG
CGCTCTAACCAAGTTAATAGGCGTTAAAAAGATTGGTCATGCAGGAACTTTAGATCCCTTCGCTACTGGCGTTATGGTCA
TGTTGATTGGCCGTAAATTTACTAGACTTTCTGATATTTTACTTTTTGAAGACAAGGAATACGAAGCAATTGCCCATTTA
GGGACAACTACCGATTCTTATGATTGCGACGGCAAAGTTGTAGGAAGATCTAAGAAGATTCCTAGTCTCGAAGAAGTATT
ATCAGCTGCCGAGTATTTCCAAGGAGAGATCCAGCAACTTCCTCCCATGTTTTCCGCTAAAAAAGTCCAAGGGAAAAAGC
TGTATGAATATGCTAGAAAAGGTTTATCTATAGAACGTCACCATTCTACAGTTCAAGTTCACTTGCAGATTACGAAATAT
GAGTACCCTTTATTGCATTTTGTAGTCTCTTGTAGCAAAGGAACTTATATTCGCAGCATTGCTCATGAGCTTGGCACGAT
GTTAGGCTGTGGAGCTTATCTTGAGCAGCTACGCCGTTTACGCAGTGGCCGTTTTTCTATAGATGAATGTATTGATGGGA
ATCTATTAGACCACCCCGATTTCGATATTTCTCCCTACCTACGAGATGCCCATGGAAATAGCCTATAG

Upstream 100 bases:

>100_bases
TCAAGATTATATAGAAAACCTGCTTTGGCAGATTCAAGAGAAAGAAAAGAGTTAATAAACTATATTTTTTGGGAACTTGA
ATACTATTAAAGACATGACT

Downstream 100 bases:

>100_bases
TTTAACGTCTTCGTTTTCTGTAGATTCTGTAACTGTAGGTTTTTTCGACGGATGTCATCTAGGGCATAGCAATCTTTTAT
CTATTCTTACTTCCTATTCT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MDLAVELKEGILLVDKPQGRTSFSLIRALTKLIGVKKIGHAGTLDPFATGVMVMLIGRKFTRLSDILLFEDKEYEAIAHL
GTTTDSYDCDGKVVGRSKKIPSLEEVLSAAEYFQGEIQQLPPMFSAKKVQGKKLYEYARKGLSIERHHSTVQVHLQITKY
EYPLLHFVVSCSKGTYIRSIAHELGTMLGCGAYLEQLRRLRSGRFSIDECIDGNLLDHPDFDISPYLRDAHGNSL

Sequences:

>Translated_235_residues
MDLAVELKEGILLVDKPQGRTSFSLIRALTKLIGVKKIGHAGTLDPFATGVMVMLIGRKFTRLSDILLFEDKEYEAIAHL
GTTTDSYDCDGKVVGRSKKIPSLEEVLSAAEYFQGEIQQLPPMFSAKKVQGKKLYEYARKGLSIERHHSTVQVHLQITKY
EYPLLHFVVSCSKGTYIRSIAHELGTMLGCGAYLEQLRRLRSGRFSIDECIDGNLLDHPDFDISPYLRDAHGNSL
>Mature_235_residues
MDLAVELKEGILLVDKPQGRTSFSLIRALTKLIGVKKIGHAGTLDPFATGVMVMLIGRKFTRLSDILLFEDKEYEAIAHL
GTTTDSYDCDGKVVGRSKKIPSLEEVLSAAEYFQGEIQQLPPMFSAKKVQGKKLYEYARKGLSIERHHSTVQVHLQITKY
EYPLLHFVVSCSKGTYIRSIAHELGTMLGCGAYLEQLRRLRSGRFSIDECIDGNLLDHPDFDISPYLRDAHGNSL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=33.1818181818182, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4503337, Length=200, Percent_Identity=31, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI215599015, Length=200, Percent_Identity=31, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI2367200, Length=203, Percent_Identity=41.3793103448276, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553978, Length=230, Percent_Identity=27.8260869565217, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=205, Percent_Identity=30.2439024390244, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=149, Percent_Identity=36.9127516778523, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364189, Length=202, Percent_Identity=31.1881188118812, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364187, Length=202, Percent_Identity=31.1881188118812, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364185, Length=202, Percent_Identity=31.1881188118812, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364183, Length=202, Percent_Identity=31.1881188118812, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62471759, Length=202, Percent_Identity=31.1881188118812, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17975520, Length=202, Percent_Identity=31.1881188118812, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CHLPN (Q9Z8L9)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   G86530
- PIR:   H72093
- RefSeq:   NP_224524.1
- RefSeq:   NP_300378.1
- RefSeq:   NP_444986.1
- RefSeq:   NP_876605.1
- ProteinModelPortal:   Q9Z8L9
- SMR:   Q9Z8L9
- GeneID:   1467012
- GeneID:   895580
- GeneID:   919095
- GeneID:   962805
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0438
- KEGG:   cpn:CPn0319
- KEGG:   cpt:CpB0329
- TIGR:   CP_0438
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- PhylomeDB:   Q9Z8L9
- ProtClustDB:   PRK00989
- BioCyc:   CPNE115711:CP_0438-MONOMER
- BioCyc:   CPNE115713:CPN0319-MONOMER
- BioCyc:   CPNE138677:CPJ0319-MONOMER
- BioCyc:   CPNE182082:CPB0329-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 26318; Mature: 26318

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAVELKEGILLVDKPQGRTSFSLIRALTKLIGVKKIGHAGTLDPFATGVMVMLIGRKF
CCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TRLSDILLFEDKEYEAIAHLGTTTDSYDCDGKVVGRSKKIPSLEEVLSAAEYFQGEIQQL
HHHHHHHEECCCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHC
PPMFSAKKVQGKKLYEYARKGLSIERHHSTVQVHLQITKYEYPLLHFVVSCSKGTYIRSI
CCCCCHHHCCHHHHHHHHHHCCCEEECCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHH
AHELGTMLGCGAYLEQLRRLRSGRFSIDECIDGNLLDHPDFDISPYLRDAHGNSL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MDLAVELKEGILLVDKPQGRTSFSLIRALTKLIGVKKIGHAGTLDPFATGVMVMLIGRKF
CCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TRLSDILLFEDKEYEAIAHLGTTTDSYDCDGKVVGRSKKIPSLEEVLSAAEYFQGEIQQL
HHHHHHHEECCCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHC
PPMFSAKKVQGKKLYEYARKGLSIERHHSTVQVHLQITKYEYPLLHFVVSCSKGTYIRSI
CCCCCHHHCCHHHHHHHHHHCCCEEECCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHH
AHELGTMLGCGAYLEQLRRLRSGRFSIDECIDGNLLDHPDFDISPYLRDAHGNSL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362