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The map label for this gene is xasA

Identifier: 15835817

GI number: 15835817

Start: 315748

End: 317154

Strand: Direct

Name: xasA

Synonym: CPj0282

Alternate gene names: 15835817

Gene position: 315748-317154 (Clockwise)

Preceding gene: 15835816

Following gene: 15835824

Centisome position: 25.74

GC content: 39.3

Gene sequence:

>1407_bases
ATGCACTCCCACTCAAAACCAACGAAACCGTTGGGAACATTCACGGTCGGCATGTTATCACTTGCTGTAGTGATTAGTTT
GCGTAATCTCCCGTTAACAGCAAAACATGGTCTTTCCACTCTGTTTTTTTATGGACTAGCAGTCATATGTTTTATGATTC
CGTATGCTCTGATTTCTGCTGAGCTTGCTTCTTTCAAGCCTCAGGGAATTTATATTTGGGCACGTGACGCTCTAGGCAAA
TGGTGGGGATTCTTTGCTATATGGATGCAATGGTTTCACAACATGACGTGGTATCCTGCCGTGTTAGCTTTTATAGCGAG
TACCATTGTTTATAAAATCAATCCAGAACTCGCTCACAACAAAGTGTACATTGCAACCGTGATCCTTGCTGGTTTTTGGA
TACTTACATTTTTTAATTTTTTAGGAATTACTTCTTCCGCATTATTCAGCTCTATTTGTGTAATCATAGGAACATTAATT
CCAGGAGTCATCTTAGTTAGTTTGGCTCTCTTTTGGATTTTTTCTGGCAATCCCATTGCTATTTCTCTTTCTTGGGGAAA
TCTTCTTCCTAATTTCAGTAACGTATCTTCACTTGTACTACTAGCTGGAATGTTACTTGCGTTATGTGGTCTAGAGGCTA
ATGCGAACCTTGCTTCTGATATGGTAAATCCTAGAAAAAATTATCCAAAGGCAGTCTTCATTGGTGCAATAGCAACACTC
ACTATTTTAGTTCTGGGTTCTTTATCCATAGCAATAGTGATTCCGAAAGAAGAAATTAGTTTAGTCTCTGGACTAGTAAA
AACGTTTACCTTGTTCTTTGATAAATATAACCTCTCCTGGATGACTGGAATCGTTGTAGTCATGACCATTGCAGGATCGC
TAGGCGAACTTAATGCTTGGATGTTTGCAGGAACAAAGGGGCTTTTTATTTCCACTCAGAATGACTGTCTTCCCCGACTC
TTTAAGAAAGTAAATAGCAAAAATGTTCCAACGAACTTAATGTTATTCCAAGGTATTGTTGTGACAATATTCACACTTTT
ATTTCTATGCCTTGATTCAGCAGACCTGGTGTATTGGATTTTAACTGCACTGAGCGTGCAGATGTATCTTGCGATGTACA
TCTGTCTGTTTCTTGCAGGACCGATCTTACGTATCAAAGAACCAAGGGCTCAACGCCTCTATTCTGTACCAGGAAAGTTT
TTGGGAATCTGTACGATGTCTATCTTAGGAATTCTCTCCTGTGCGTTTGCTCTTTGGGTGAGCTTCCTGCCTCCTAGAGA
ACTTGCTCAGATATCTGAAGGCAGCAAAATAGGATATACTACATTCCTGCTTTTAGCATTTAGCTTGAATTGCTTAATTC
CTTTCGGAATCTATTTCACGCATAAACGCTTATCTAAAAAGAGCTAA

Upstream 100 bases:

>100_bases
CAACGTCCCCTATCCGAAGGCATCCAATTATTAAACCTGGTTCAAGATATCTATTTAGATCCTAATATTACAATCGCTTA
ACTTTTCAAAGAAGGTCTTT

Downstream 100 bases:

>100_bases
TCTAAAAGCATTTTTGGGAAAAGAAAAGAAAGAAGCCTTCCTTGTTGTATGGCAGCCTGGAAAGCTTCTGGATTCAAATT
TGCTCTCCTGCAAAAAGTTT

Product: amino acid transporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MHSHSKPTKPLGTFTVGMLSLAVVISLRNLPLTAKHGLSTLFFYGLAVICFMIPYALISAELASFKPQGIYIWARDALGK
WWGFFAIWMQWFHNMTWYPAVLAFIASTIVYKINPELAHNKVYIATVILAGFWILTFFNFLGITSSALFSSICVIIGTLI
PGVILVSLALFWIFSGNPIAISLSWGNLLPNFSNVSSLVLLAGMLLALCGLEANANLASDMVNPRKNYPKAVFIGAIATL
TILVLGSLSIAIVIPKEEISLVSGLVKTFTLFFDKYNLSWMTGIVVVMTIAGSLGELNAWMFAGTKGLFISTQNDCLPRL
FKKVNSKNVPTNLMLFQGIVVTIFTLLFLCLDSADLVYWILTALSVQMYLAMYICLFLAGPILRIKEPRAQRLYSVPGKF
LGICTMSILGILSCAFALWVSFLPPRELAQISEGSKIGYTTFLLLAFSLNCLIPFGIYFTHKRLSKKS

Sequences:

>Translated_468_residues
MHSHSKPTKPLGTFTVGMLSLAVVISLRNLPLTAKHGLSTLFFYGLAVICFMIPYALISAELASFKPQGIYIWARDALGK
WWGFFAIWMQWFHNMTWYPAVLAFIASTIVYKINPELAHNKVYIATVILAGFWILTFFNFLGITSSALFSSICVIIGTLI
PGVILVSLALFWIFSGNPIAISLSWGNLLPNFSNVSSLVLLAGMLLALCGLEANANLASDMVNPRKNYPKAVFIGAIATL
TILVLGSLSIAIVIPKEEISLVSGLVKTFTLFFDKYNLSWMTGIVVVMTIAGSLGELNAWMFAGTKGLFISTQNDCLPRL
FKKVNSKNVPTNLMLFQGIVVTIFTLLFLCLDSADLVYWILTALSVQMYLAMYICLFLAGPILRIKEPRAQRLYSVPGKF
LGICTMSILGILSCAFALWVSFLPPRELAQISEGSKIGYTTFLLLAFSLNCLIPFGIYFTHKRLSKKS
>Mature_468_residues
MHSHSKPTKPLGTFTVGMLSLAVVISLRNLPLTAKHGLSTLFFYGLAVICFMIPYALISAELASFKPQGIYIWARDALGK
WWGFFAIWMQWFHNMTWYPAVLAFIASTIVYKINPELAHNKVYIATVILAGFWILTFFNFLGITSSALFSSICVIIGTLI
PGVILVSLALFWIFSGNPIAISLSWGNLLPNFSNVSSLVLLAGMLLALCGLEANANLASDMVNPRKNYPKAVFIGAIATL
TILVLGSLSIAIVIPKEEISLVSGLVKTFTLFFDKYNLSWMTGIVVVMTIAGSLGELNAWMFAGTKGLFISTQNDCLPRL
FKKVNSKNVPTNLMLFQGIVVTIFTLLFLCLDSADLVYWILTALSVQMYLAMYICLFLAGPILRIKEPRAQRLYSVPGKF
LGICTMSILGILSCAFALWVSFLPPRELAQISEGSKIGYTTFLLLAFSLNCLIPFGIYFTHKRLSKKS

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=458, Percent_Identity=29.6943231441048, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI87081798, Length=443, Percent_Identity=22.3476297968397, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 51640; Mature: 51640

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSHSKPTKPLGTFTVGMLSLAVVISLRNLPLTAKHGLSTLFFYGLAVICFMIPYALISA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELASFKPQGIYIWARDALGKWWGFFAIWMQWFHNMTWYPAVLAFIASTIVYKINPELAHN
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCHHCC
KVYIATVILAGFWILTFFNFLGITSSALFSSICVIIGTLIPGVILVSLALFWIFSGNPIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ISLSWGNLLPNFSNVSSLVLLAGMLLALCGLEANANLASDMVNPRKNYPKAVFIGAIATL
EEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
TILVLGSLSIAIVIPKEEISLVSGLVKTFTLFFDKYNLSWMTGIVVVMTIAGSLGELNAW
HHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHH
MFAGTKGLFISTQNDCLPRLFKKVNSKNVPTNLMLFQGIVVTIFTLLFLCLDSADLVYWI
EEECCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTALSVQMYLAMYICLFLAGPILRIKEPRAQRLYSVPGKFLGICTMSILGILSCAFALWV
HHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SFLPPRELAQISEGSKIGYTTFLLLAFSLNCLIPFGIYFTHKRLSKKS
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHSHSKPTKPLGTFTVGMLSLAVVISLRNLPLTAKHGLSTLFFYGLAVICFMIPYALISA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELASFKPQGIYIWARDALGKWWGFFAIWMQWFHNMTWYPAVLAFIASTIVYKINPELAHN
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCHHCC
KVYIATVILAGFWILTFFNFLGITSSALFSSICVIIGTLIPGVILVSLALFWIFSGNPIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ISLSWGNLLPNFSNVSSLVLLAGMLLALCGLEANANLASDMVNPRKNYPKAVFIGAIATL
EEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
TILVLGSLSIAIVIPKEEISLVSGLVKTFTLFFDKYNLSWMTGIVVVMTIAGSLGELNAW
HHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHH
MFAGTKGLFISTQNDCLPRLFKKVNSKNVPTNLMLFQGIVVTIFTLLFLCLDSADLVYWI
EEECCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTALSVQMYLAMYICLFLAGPILRIKEPRAQRLYSVPGKFLGICTMSILGILSCAFALWV
HHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SFLPPRELAQISEGSKIGYTTFLLLAFSLNCLIPFGIYFTHKRLSKKS
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]