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The map label for this gene is pfkA_2

Identifier: 15835743

GI number: 15835743

Start: 248568

End: 250220

Strand: Direct

Name: pfkA_2

Synonym: CPj0208

Alternate gene names: 15835743

Gene position: 248568-250220 (Clockwise)

Preceding gene: 15835742

Following gene: 15835744

Centisome position: 20.27

GC content: 42.04

Gene sequence:

>1653_bases
ATGCATCCTTTATACGTTGATCTTGATACTATTATCAGCTCCTACTCTCCTCCCTTACCTAAAGAATTTCAAGAAGCAGC
CTCTTTAATTGCTGTTCCAGATACTTCACATTCTAAGCCTGTCGTTCCAGGAGTGAAAACCCTCTTTCCACAAACCTACC
ACCTTCCCTATCTAAAGTTTGTCCAAGGAGAAAATGTCGTTCACACTCCTCTAAAAGTAGGCGTAATGTTCTCAGGAGGA
CCTGCTCCAGGAGGACATAATGTCATCCAAGGACTCTTCAATAGTCTAAAAGATTTCCATCCCGATTCCTCCCTCGTGGG
GTTCGTAAATAATGGAGACGGTCTTACAAACAATAAAAGCATAGACATTACTGAAGAGTTTCTCTCCAAATTCCGAAATT
CCGGAGGCTTCAACTGTATAGGAACAGGAAGGAAAAAAATTGTAACTCCAGAAGCTAAAGAGGCTTGTCTAAAGACCGCA
GAGGCTCTGGATCTCGACGGACTAGTCATTATTGGCGGTGATGGCTCCAATACAGCAACCGCTATTCTTGCAGAGTATTT
TGCAAAACGACGCCCAAAAACCTCTATTGTCGGAGTTCCTAAAACTATAGATGGGGATCTACAACACACCTTCTTGGATC
TGACCTTCGGATTTGATACTGCAACAAAATTCTACTCTTCAATCATTAGCAATATTTCAAGAGATGCTCTTTCCTGTAAA
GCTCATTACCACTTCATTAAACTTATGGGACGCTCAGCATCCCATATTGCTTTGGAATGTGCTCTCCAAACTCATCCAAA
TATTGCCCTTATCGGCGAAGAAATTGCCGAAAAAAATCTACCACTAAAAACCATCATCCATAAAATCTGCTCCGTAATTG
CAGATAGAGCCGCTATGGAAAAATACTATGGCGTCATCCTCATCCCAGAAGGCATTATCGAGTTCATCCCAGAAATCATC
AACTTAATTACAGAAATCGAAAGCCTATCAGAATACGAAGATAAAATCTCCAGGCTCTCTCCAGAATCCCAACGCCTACT
GAAAAGCTTCCCAGCACCTATCATCGAGCAAATCCTCAATGACCGCGATGCTCACGGTAATGTCTATGTTTCTAAAATTA
GTGTCGATAAACTACTCATCCACCTGGTCAGCAATCATCTCCAACAATATTTCCCTAACGTCCCTTTCAATGCGATCTCA
CATTTTCTAGGATATGAAGGACGCTCGGGATTGCCTACAAAATTCGATAATACCTACGGCTATAGCCTCGGATACGGCGC
CGGTATTCTCGTCCGCAATCACTGCAACGGCTATCTCTCTACTATAGAATCCCTAGCATGCCCTTTCATGAAATGGAAAT
TACGGGCAATTCCCGTAGTGAAAATGTTCACAGTAAAACAACAGGCAGATGGAACTCTACAACCTAAAATTAAAAAATAC
CTCGTAGATATAGGAAGCACGGCATTTCGTAAATTTAAGCTCTATAGGAAAATTTGGGCCCTCGAAGACTCCTACCGATT
CCTAGGGCCTCTACAAATAGAAACTCCTCCAGAAATGCACTCTGATAATTTCCCTCCTCTTACCCTTTTGCTTAATCATA
ACTTTTGGCAACGTCACCAGGGTTGCATAGAAATCCCTGATACTACGTATTAA

Upstream 100 bases:

>100_bases
TTTTTAAAAAAACCTGATGTTCTTATGCACAAAAAGAATCCCATTGACTTTAGCCTTGGGATGATGCAAAAAGCAAAATT
AGAGTGTAGCCGTTATCCTT

Downstream 100 bases:

>100_bases
TTACGTTCTAATACGTTCTTAATTCCTGAAAATCTAAGATGCTTCCACGCAAGCTTATCCGCATACTAAACTTAGCAGAA
GTAATGGATCTGATTCGGAT

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MHPLYVDLDTIISSYSPPLPKEFQEAASLIAVPDTSHSKPVVPGVKTLFPQTYHLPYLKFVQGENVVHTPLKVGVMFSGG
PAPGGHNVIQGLFNSLKDFHPDSSLVGFVNNGDGLTNNKSIDITEEFLSKFRNSGGFNCIGTGRKKIVTPEAKEACLKTA
EALDLDGLVIIGGDGSNTATAILAEYFAKRRPKTSIVGVPKTIDGDLQHTFLDLTFGFDTATKFYSSIISNISRDALSCK
AHYHFIKLMGRSASHIALECALQTHPNIALIGEEIAEKNLPLKTIIHKICSVIADRAAMEKYYGVILIPEGIIEFIPEII
NLITEIESLSEYEDKISRLSPESQRLLKSFPAPIIEQILNDRDAHGNVYVSKISVDKLLIHLVSNHLQQYFPNVPFNAIS
HFLGYEGRSGLPTKFDNTYGYSLGYGAGILVRNHCNGYLSTIESLACPFMKWKLRAIPVVKMFTVKQQADGTLQPKIKKY
LVDIGSTAFRKFKLYRKIWALEDSYRFLGPLQIETPPEMHSDNFPPLTLLLNHNFWQRHQGCIEIPDTTY

Sequences:

>Translated_550_residues
MHPLYVDLDTIISSYSPPLPKEFQEAASLIAVPDTSHSKPVVPGVKTLFPQTYHLPYLKFVQGENVVHTPLKVGVMFSGG
PAPGGHNVIQGLFNSLKDFHPDSSLVGFVNNGDGLTNNKSIDITEEFLSKFRNSGGFNCIGTGRKKIVTPEAKEACLKTA
EALDLDGLVIIGGDGSNTATAILAEYFAKRRPKTSIVGVPKTIDGDLQHTFLDLTFGFDTATKFYSSIISNISRDALSCK
AHYHFIKLMGRSASHIALECALQTHPNIALIGEEIAEKNLPLKTIIHKICSVIADRAAMEKYYGVILIPEGIIEFIPEII
NLITEIESLSEYEDKISRLSPESQRLLKSFPAPIIEQILNDRDAHGNVYVSKISVDKLLIHLVSNHLQQYFPNVPFNAIS
HFLGYEGRSGLPTKFDNTYGYSLGYGAGILVRNHCNGYLSTIESLACPFMKWKLRAIPVVKMFTVKQQADGTLQPKIKKY
LVDIGSTAFRKFKLYRKIWALEDSYRFLGPLQIETPPEMHSDNFPPLTLLLNHNFWQRHQGCIEIPDTTY
>Mature_550_residues
MHPLYVDLDTIISSYSPPLPKEFQEAASLIAVPDTSHSKPVVPGVKTLFPQTYHLPYLKFVQGENVVHTPLKVGVMFSGG
PAPGGHNVIQGLFNSLKDFHPDSSLVGFVNNGDGLTNNKSIDITEEFLSKFRNSGGFNCIGTGRKKIVTPEAKEACLKTA
EALDLDGLVIIGGDGSNTATAILAEYFAKRRPKTSIVGVPKTIDGDLQHTFLDLTFGFDTATKFYSSIISNISRDALSCK
AHYHFIKLMGRSASHIALECALQTHPNIALIGEEIAEKNLPLKTIIHKICSVIADRAAMEKYYGVILIPEGIIEFIPEII
NLITEIESLSEYEDKISRLSPESQRLLKSFPAPIIEQILNDRDAHGNVYVSKISVDKLLIHLVSNHLQQYFPNVPFNAIS
HFLGYEGRSGLPTKFDNTYGYSLGYGAGILVRNHCNGYLSTIESLACPFMKWKLRAIPVVKMFTVKQQADGTLQPKIKKY
LVDIGSTAFRKFKLYRKIWALEDSYRFLGPLQIETPPEMHSDNFPPLTLLLNHNFWQRHQGCIEIPDTTY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=196, Percent_Identity=29.0816326530612, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 61181; Mature: 61181

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPLYVDLDTIISSYSPPLPKEFQEAASLIAVPDTSHSKPVVPGVKTLFPQTYHLPYLKF
CCCEEEEHHHHHHCCCCCCCHHHHHHHCEEECCCCCCCCCCCCCHHHHCCCCCCCCHHEE
VQGENVVHTPLKVGVMFSGGPAPGGHNVIQGLFNSLKDFHPDSSLVGFVNNGDGLTNNKS
ECCCCEEECCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCC
IDITEEFLSKFRNSGGFNCIGTGRKKIVTPEAKEACLKTAEALDLDGLVIIGGDGSNTAT
CCHHHHHHHHHHCCCCCEEEECCCCEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
AILAEYFAKRRPKTSIVGVPKTIDGDLQHTFLDLTFGFDTATKFYSSIISNISRDALSCK
HHHHHHHHHCCCCCCEEECCCCCCCCHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AHYHFIKLMGRSASHIALECALQTHPNIALIGEEIAEKNLPLKTIIHKICSVIADRAAME
HHHHHHHHHCCCCCEEEEEEEECCCCCEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KYYGVILIPEGIIEFIPEIINLITEIESLSEYEDKISRLSPESQRLLKSFPAPIIEQILN
HHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHC
DRDAHGNVYVSKISVDKLLIHLVSNHLQQYFPNVPFNAISHFLGYEGRSGLPTKFDNTYG
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
YSLGYGAGILVRNHCNGYLSTIESLACPFMKWKLRAIPVVKMFTVKQQADGTLQPKIKKY
CCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHHHHCCEEHEEEEHHHCCCCCCHHHHHH
LVDIGSTAFRKFKLYRKIWALEDSYRFLGPLQIETPPEMHSDNFPPLTLLLNHNFWQRHQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECCCCCCCCCCCCCEEEEECCCHHHHCC
GCIEIPDTTY
CCCCCCCCCC
>Mature Secondary Structure
MHPLYVDLDTIISSYSPPLPKEFQEAASLIAVPDTSHSKPVVPGVKTLFPQTYHLPYLKF
CCCEEEEHHHHHHCCCCCCCHHHHHHHCEEECCCCCCCCCCCCCHHHHCCCCCCCCHHEE
VQGENVVHTPLKVGVMFSGGPAPGGHNVIQGLFNSLKDFHPDSSLVGFVNNGDGLTNNKS
ECCCCEEECCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCC
IDITEEFLSKFRNSGGFNCIGTGRKKIVTPEAKEACLKTAEALDLDGLVIIGGDGSNTAT
CCHHHHHHHHHHCCCCCEEEECCCCEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
AILAEYFAKRRPKTSIVGVPKTIDGDLQHTFLDLTFGFDTATKFYSSIISNISRDALSCK
HHHHHHHHHCCCCCCEEECCCCCCCCHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AHYHFIKLMGRSASHIALECALQTHPNIALIGEEIAEKNLPLKTIIHKICSVIADRAAME
HHHHHHHHHCCCCCEEEEEEEECCCCCEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KYYGVILIPEGIIEFIPEIINLITEIESLSEYEDKISRLSPESQRLLKSFPAPIIEQILN
HHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHC
DRDAHGNVYVSKISVDKLLIHLVSNHLQQYFPNVPFNAISHFLGYEGRSGLPTKFDNTYG
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
YSLGYGAGILVRNHCNGYLSTIESLACPFMKWKLRAIPVVKMFTVKQQADGTLQPKIKKY
CCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHHHHCCEEHEEEEHHHCCCCCCHHHHHH
LVDIGSTAFRKFKLYRKIWALEDSYRFLGPLQIETPPEMHSDNFPPLTLLLNHNFWQRHQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECCCCCCCCCCCCCEEEEECCCHHHHCC
GCIEIPDTTY
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]