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The map label for this gene is ligA

Identifier: 15835684

GI number: 15835684

Start: 185426

End: 187414

Strand: Direct

Name: ligA

Synonym: CPj0149

Alternate gene names: 15835684

Gene position: 185426-187414 (Clockwise)

Preceding gene: 15835683

Following gene: 15835685

Centisome position: 15.12

GC content: 41.43

Gene sequence:

>1989_bases
ATGAAGGAAGAGAATTCACAAGCACACTACTTAGCTTTATGTCGTGAATTAGAAGACCATGATTATTCTTATTATGTGTT
GCATCGTCCTAGAATCTCTGATTATGAATATGACATGAAATTACGGAAGCTTCTTGAAATAGAGAGAAGTCATCCCGAAT
GGAAAGTCTTATGGTCTCCCTCAACACGTCTCGGAGATCGTCCCTCTGGAACTTTTTCTGTGGTTTCCCATAAGGAACCG
ATGCTTTCCATTGCCAATAGCTATTCTAAAGAAGAACTAAGTGAGTTTTTTTCTAGGGTAGAAAAATCCCTAGGTACAAG
TCCACGTTATACAGTAGAACTTAAAATCGATGGGATTGCAGTAGCAATACGTTATGAAGATCGTGTGTTGGTTCAAGCAC
TCAGCCGAGGAAATGGAAAGCAGGGAGAGGATATCACATCGAATATTCGAACAATACGCTCCTTGCCTTTAAGACTTCCA
GAAGATGCTCCAGAGTTTATTGAAGTACGTGGCGAGGTCTTCTTCTCTTATTCTACGTTTCAAATTATCAATGAGAAGCA
GCAACAATTAGAGAAAACTATTTTTGCCAACCCGAGAAATGCTGCAGGAGGTACCTTAAAGTTACTTTCTCCTCAAGAAG
TCGCAAAACGTAAATTAGAAATTTCTATCTATAATCTCATTGCTCCAGGAGATAACGATTCTCATTATGAAAATCTTCAG
CGCTGCCTTGAATGGGGATTTCCTGTATCTGGTAAACCAAGATTGTGCTCTACCCCAGAGGAAGTGATCTCAGTTTTAAA
GACTATAGAAACTGAGAGAGCTTCCTTGCCTATGGAAATCGATGGTGCTGTCATCAAGGTAGACAGTTTGGCAAGTCAGA
GAGTTCTTGGAGCCACAGGGAAACACTATAGATGGGCCTTAGCTTATAAATATGCCCCAGAGGAAGCAGAGACCCTTCTT
GAGGATATTCTAGTTCAAGTAGGAAGAACGGGAGTTCTGACTCCTGTAGCTAAACTCACTCCTGTACTGTTGTCAGGGTC
TTTAGTATCTAGAGCGTCTCTATACAATGAAGATGAGATTCATAGAAAAGACATCCGTATTGGTGATACCGTTTGTGTTG
CTAAAGGTGGAGAGGTGATTCCAAAAGTAGTTCGGGTATGCAGAGAAAAACGTCCTGAAGGTTCTGAAGTTTGGAATATG
CCTGAATTCTGCCCTGTCTGCCATAGTCACGTAGTTCGGGAAGAAGATAGAGTTTCTGTGCGTTGTGTCAATCCTGAGTG
TGTTGCAGGAGCTATTGAAAAAATTCGTTTTTTTGTTGGTCGGGGAGCTTTAAATATCGATCATTTAGGGGTGAAGGTAA
TCACAAAGCTGTTTGAATTAGGGTTAGTGCACACGTGTGCGGACCTATTTCAGCTGACTACTGAAGATTTAATGCAAATT
CCCGGGATACGGGAACGCTCTGCAAGAAATATTCTAGAGAGTATCGAGCAAGCTAAACATGTGGATCTAGATCGTTTTCT
TGTTGCTCTGGGGATTCCTCTCATTGGAATTGGTGTTGCTACTGTACTAGCTGGCCACTTCGAGACTTTAGATCGGGTAA
TTTCCGCGACTTTTGAAGAACTTCTTTCACTAGAGGGTATTGGAGAGAAGGTGGCTCATGCTATTGCTGAGTATTTTTCA
GACTCTACGCATCTTAACGAAATCAAGAAAATGCAGGATTTAGGAGTGTGTATATCTCCTTATCATAAATCAGGATCTAC
GTGTTTTGGCAAGGCTTTTGTGATCACAGGGACGTTAGAGGGAATGTCTCGGTTAGATGCAGAAACTGCTATCCGGAATT
GTGGGGGTAAGGTAGGCTCCTCTGTCTCGAAACAGACCGATTACGTAGTTATGGGGAATAACCCAGGATCTAAATTAGAG
AAGGCTAGGAAATTGGGAGTCTCTATCTTAGATCAAGAAGCCTTTACAAATCTAATTCATTTAGAATAA

Upstream 100 bases:

>100_bases
AGATGATCTTCGCTGTGCTCATCGCCATCGTAATAATCCTGGGGCTGTAAATAGTACGTATGGTTTTAGGTGCGCTAAAA
ATATCAATTAAGAGAGGTTC

Downstream 100 bases:

>100_bases
TTTATTTTAAAATTTTCTTAATACATTAATTCTTATTTGTAAAAGTTTTATTTAAATTATTTATTATAAATTCTTTTACA
GCTATAATTGTCCGTATTTT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MKEENSQAHYLALCRELEDHDYSYYVLHRPRISDYEYDMKLRKLLEIERSHPEWKVLWSPSTRLGDRPSGTFSVVSHKEP
MLSIANSYSKEELSEFFSRVEKSLGTSPRYTVELKIDGIAVAIRYEDRVLVQALSRGNGKQGEDITSNIRTIRSLPLRLP
EDAPEFIEVRGEVFFSYSTFQIINEKQQQLEKTIFANPRNAAGGTLKLLSPQEVAKRKLEISIYNLIAPGDNDSHYENLQ
RCLEWGFPVSGKPRLCSTPEEVISVLKTIETERASLPMEIDGAVIKVDSLASQRVLGATGKHYRWALAYKYAPEEAETLL
EDILVQVGRTGVLTPVAKLTPVLLSGSLVSRASLYNEDEIHRKDIRIGDTVCVAKGGEVIPKVVRVCREKRPEGSEVWNM
PEFCPVCHSHVVREEDRVSVRCVNPECVAGAIEKIRFFVGRGALNIDHLGVKVITKLFELGLVHTCADLFQLTTEDLMQI
PGIRERSARNILESIEQAKHVDLDRFLVALGIPLIGIGVATVLAGHFETLDRVISATFEELLSLEGIGEKVAHAIAEYFS
DSTHLNEIKKMQDLGVCISPYHKSGSTCFGKAFVITGTLEGMSRLDAETAIRNCGGKVGSSVSKQTDYVVMGNNPGSKLE
KARKLGVSILDQEAFTNLIHLE

Sequences:

>Translated_662_residues
MKEENSQAHYLALCRELEDHDYSYYVLHRPRISDYEYDMKLRKLLEIERSHPEWKVLWSPSTRLGDRPSGTFSVVSHKEP
MLSIANSYSKEELSEFFSRVEKSLGTSPRYTVELKIDGIAVAIRYEDRVLVQALSRGNGKQGEDITSNIRTIRSLPLRLP
EDAPEFIEVRGEVFFSYSTFQIINEKQQQLEKTIFANPRNAAGGTLKLLSPQEVAKRKLEISIYNLIAPGDNDSHYENLQ
RCLEWGFPVSGKPRLCSTPEEVISVLKTIETERASLPMEIDGAVIKVDSLASQRVLGATGKHYRWALAYKYAPEEAETLL
EDILVQVGRTGVLTPVAKLTPVLLSGSLVSRASLYNEDEIHRKDIRIGDTVCVAKGGEVIPKVVRVCREKRPEGSEVWNM
PEFCPVCHSHVVREEDRVSVRCVNPECVAGAIEKIRFFVGRGALNIDHLGVKVITKLFELGLVHTCADLFQLTTEDLMQI
PGIRERSARNILESIEQAKHVDLDRFLVALGIPLIGIGVATVLAGHFETLDRVISATFEELLSLEGIGEKVAHAIAEYFS
DSTHLNEIKKMQDLGVCISPYHKSGSTCFGKAFVITGTLEGMSRLDAETAIRNCGGKVGSSVSKQTDYVVMGNNPGSKLE
KARKLGVSILDQEAFTNLIHLE
>Mature_662_residues
MKEENSQAHYLALCRELEDHDYSYYVLHRPRISDYEYDMKLRKLLEIERSHPEWKVLWSPSTRLGDRPSGTFSVVSHKEP
MLSIANSYSKEELSEFFSRVEKSLGTSPRYTVELKIDGIAVAIRYEDRVLVQALSRGNGKQGEDITSNIRTIRSLPLRLP
EDAPEFIEVRGEVFFSYSTFQIINEKQQQLEKTIFANPRNAAGGTLKLLSPQEVAKRKLEISIYNLIAPGDNDSHYENLQ
RCLEWGFPVSGKPRLCSTPEEVISVLKTIETERASLPMEIDGAVIKVDSLASQRVLGATGKHYRWALAYKYAPEEAETLL
EDILVQVGRTGVLTPVAKLTPVLLSGSLVSRASLYNEDEIHRKDIRIGDTVCVAKGGEVIPKVVRVCREKRPEGSEVWNM
PEFCPVCHSHVVREEDRVSVRCVNPECVAGAIEKIRFFVGRGALNIDHLGVKVITKLFELGLVHTCADLFQLTTEDLMQI
PGIRERSARNILESIEQAKHVDLDRFLVALGIPLIGIGVATVLAGHFETLDRVISATFEELLSLEGIGEKVAHAIAEYFS
DSTHLNEIKKMQDLGVCISPYHKSGSTCFGKAFVITGTLEGMSRLDAETAIRNCGGKVGSSVSKQTDYVVMGNNPGSKLE
KARKLGVSILDQEAFTNLIHLE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=79, Percent_Identity=46.8354430379747, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788750, Length=655, Percent_Identity=41.8320610687023, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI87082305, Length=573, Percent_Identity=22.6876090750436, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CHLPN (Q9Z934)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72114
- PIR:   E86509
- RefSeq:   NP_224357.1
- RefSeq:   NP_300208.1
- RefSeq:   NP_445166.1
- RefSeq:   NP_876426.1
- ProteinModelPortal:   Q9Z934
- SMR:   Q9Z934
- GeneID:   1466833
- GeneID:   894944
- GeneID:   918940
- GeneID:   963280
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0624
- KEGG:   cpn:CPn0149
- KEGG:   cpt:CpB0150
- TIGR:   CP_0624
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q9Z934
- ProtClustDB:   PRK07956
- BioCyc:   CPNE115711:CP_0624-MONOMER
- BioCyc:   CPNE115713:CPN0149-MONOMER
- BioCyc:   CPNE138677:CPJ0149-MONOMER
- BioCyc:   CPNE182082:CPB0150-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73911; Mature: 73911

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 172-172 BINDING 286-286 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEENSQAHYLALCRELEDHDYSYYVLHRPRISDYEYDMKLRKLLEIERSHPEWKVLWSP
CCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
STRLGDRPSGTFSVVSHKEPMLSIANSYSKEELSEFFSRVEKSLGTSPRYTVELKIDGIA
CCCCCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCEE
VAIRYEDRVLVQALSRGNGKQGEDITSNIRTIRSLPLRLPEDAPEFIEVRGEVFFSYSTF
EEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEEHHH
QIINEKQQQLEKTIFANPRNAAGGTLKLLSPQEVAKRKLEISIYNLIAPGDNDSHYENLQ
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHEEEEEEEECCCCCCHHHHHHH
RCLEWGFPVSGKPRLCSTPEEVISVLKTIETERASLPMEIDGAVIKVDSLASQRVLGATG
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEEHHHHHHHHHCCCC
KHYRWALAYKYAPEEAETLLEDILVQVGRTGVLTPVAKLTPVLLSGSLVSRASLYNEDEI
CCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCHHH
HRKDIRIGDTVCVAKGGEVIPKVVRVCREKRPEGSEVWNMPEFCPVCHSHVVREEDRVSV
HHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
RCVNPECVAGAIEKIRFFVGRGALNIDHLGVKVITKLFELGLVHTCADLFQLTTEDLMQI
EECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGIRERSARNILESIEQAKHVDLDRFLVALGIPLIGIGVATVLAGHFETLDRVISATFEE
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSLEGIGEKVAHAIAEYFSDSTHLNEIKKMQDLGVCISPYHKSGSTCFGKAFVITGTLE
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCCCEECEEEEEEECHH
GMSRLDAETAIRNCGGKVGSSVSKQTDYVVMGNNPGSKLEKARKLGVSILDQEAFTNLIH
HHHHHHHHHHHHHCCCHHCCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHC
LE
CC
>Mature Secondary Structure
MKEENSQAHYLALCRELEDHDYSYYVLHRPRISDYEYDMKLRKLLEIERSHPEWKVLWSP
CCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
STRLGDRPSGTFSVVSHKEPMLSIANSYSKEELSEFFSRVEKSLGTSPRYTVELKIDGIA
CCCCCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCEE
VAIRYEDRVLVQALSRGNGKQGEDITSNIRTIRSLPLRLPEDAPEFIEVRGEVFFSYSTF
EEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEEHHH
QIINEKQQQLEKTIFANPRNAAGGTLKLLSPQEVAKRKLEISIYNLIAPGDNDSHYENLQ
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHEEEEEEEECCCCCCHHHHHHH
RCLEWGFPVSGKPRLCSTPEEVISVLKTIETERASLPMEIDGAVIKVDSLASQRVLGATG
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEEHHHHHHHHHCCCC
KHYRWALAYKYAPEEAETLLEDILVQVGRTGVLTPVAKLTPVLLSGSLVSRASLYNEDEI
CCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCHHH
HRKDIRIGDTVCVAKGGEVIPKVVRVCREKRPEGSEVWNMPEFCPVCHSHVVREEDRVSV
HHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
RCVNPECVAGAIEKIRFFVGRGALNIDHLGVKVITKLFELGLVHTCADLFQLTTEDLMQI
EECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGIRERSARNILESIEQAKHVDLDRFLVALGIPLIGIGVATVLAGHFETLDRVISATFEE
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSLEGIGEKVAHAIAEYFSDSTHLNEIKKMQDLGVCISPYHKSGSTCFGKAFVITGTLE
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCCCEECEEEEEEECHH
GMSRLDAETAIRNCGGKVGSSVSKQTDYVVMGNNPGSKLEKARKLGVSILDQEAFTNLIH
HHHHHHHHHHHHHCCCHHCCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHC
LE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362