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The map label for this gene is hemL

Identifier: 15835673

GI number: 15835673

Start: 172408

End: 173730

Strand: Reverse

Name: hemL

Synonym: CPj0138

Alternate gene names: 15835673

Gene position: 173730-172408 (Counterclockwise)

Preceding gene: 15835674

Following gene: 15835672

Centisome position: 14.16

GC content: 43.31

Gene sequence:

>1323_bases
ATGTTGAACTGCTCAAATCAAAAGCATACCGTTACTTTTGAAGAGGCATGCCAAGTCTTCCCAGGAGGCGTGAACTCTCC
CGTTCGGGCCTGCCGTTCTGTAGGAGTCACACCCCCTATAGTGAGCTCAGCACAAGGAGATATTTTCCTAGATACTCACG
GACGAGAGTTTATTGATTTTTGTGGAGGCTGGGGAGCTTTAATTCACGGCCACAGTCATCCCAAAATTGTCAAGGCTATC
CAAAAAACAGCTCTAAAAGGAACCTCCTATGGCTTAACCTCTGAAGAGGAAATTCTATTTGCAACAATGCTCCTCTCCTC
GCTCAAACTCAAAGAACATAAAATCCGTTTTGTATCCTCGGGAACGGAAGCAACAATGACTGCAGTACGTCTCGCTCGAG
GAATCACAAACCGCTCTATTATTATCAAATTTATAGGGGGATATCACGGTCATGCAGATACTCTTCTTGGAGGCATCTCA
ACAACTGAAGAAACTATAGACAACCTAACTTCATTGATACACACGCCCTCTCCACATTCTTTATTGATATCCCTGCCTTA
TAACAATAGCCAAATCCTACACCATGTCATGGAAGCTCTAGGACCTCAAGTAGCAGGAATTATCTTTGAACCTATATGTG
CAAATATGGGTATCGTCCTTCCCAAAGCCGAATTTCTAGACGATATCATAGAACTCTGCAAACGCTTCGGAAGTCTTTCT
ATTATGGATGAAGTCGTCACAGGATTTCGAGTGGCATTCCAAGGAGCTAAAGATATTTTCAATCTCTCCCCCGATATTAC
CATCTATGGAAAAATCTTAGGAGGAGGCCTACCTGCTGCAGCCCTTGTAGGACACCGCTCAATTCTCGATCACCTCATGC
CCGAAGGCACCATATTCCAAGCAGGTACCATGTCTGGGAACTTCCTCGCTATGGCTACAGGACACGCCGCCATTCAATTA
TGCCAATCCGAAGGATTCTATGACCATCTCAGCCAATTAGAGGCTCTCTTTTATTCTCCAATTGAAGAGGAAATCCGATC
TCAAGGATTCCCAGTATCTCTAGTACATCAGGGAACGATGTTCAGCCTCTTTTTTACAGAATCTGCACCCACAAATTTTG
ATGAAGCAAAAAATTCCGATGTAGAGAAATTTCAAACCTTCTACTCTGAAGTATTTGATAATGGAGTCTACCTTTCTCCA
TCTCCCCTCGAAGCAAACTTCATTAGCTCCGCACATACCGAAGAAAACCTAACTTACGCACAGAACATCATTATCGATAG
CCTGATTAAAATCTTTGACTCTTCGGCTCAAAGATTTTTCTAA

Upstream 100 bases:

>100_bases
ACAGTCCCTGACAATCTATTGCTAAACTAACTCACGGCTTTTTCTAGCCATTAAAGATCAGCTTCTCCAAAATATGTGGA
AACTCACTAAAAGAAATTCC

Downstream 100 bases:

>100_bases
TAGTCTCTGCTTTTCACATGCCAGGGCTCATCTTCATACTCTTTCTGAAAATTAGATAGAGATGATAAAACTTAATACGT
TGGAAAAAATCTCATCTGCG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLNCSNQKHTVTFEEACQVFPGGVNSPVRACRSVGVTPPIVSSAQGDIFLDTHGREFIDFCGGWGALIHGHSHPKIVKAI
QKTALKGTSYGLTSEEEILFATMLLSSLKLKEHKIRFVSSGTEATMTAVRLARGITNRSIIIKFIGGYHGHADTLLGGIS
TTEETIDNLTSLIHTPSPHSLLISLPYNNSQILHHVMEALGPQVAGIIFEPICANMGIVLPKAEFLDDIIELCKRFGSLS
IMDEVVTGFRVAFQGAKDIFNLSPDITIYGKILGGGLPAAALVGHRSILDHLMPEGTIFQAGTMSGNFLAMATGHAAIQL
CQSEGFYDHLSQLEALFYSPIEEEIRSQGFPVSLVHQGTMFSLFFTESAPTNFDEAKNSDVEKFQTFYSEVFDNGVYLSP
SPLEANFISSAHTEENLTYAQNIIIDSLIKIFDSSAQRFF

Sequences:

>Translated_440_residues
MLNCSNQKHTVTFEEACQVFPGGVNSPVRACRSVGVTPPIVSSAQGDIFLDTHGREFIDFCGGWGALIHGHSHPKIVKAI
QKTALKGTSYGLTSEEEILFATMLLSSLKLKEHKIRFVSSGTEATMTAVRLARGITNRSIIIKFIGGYHGHADTLLGGIS
TTEETIDNLTSLIHTPSPHSLLISLPYNNSQILHHVMEALGPQVAGIIFEPICANMGIVLPKAEFLDDIIELCKRFGSLS
IMDEVVTGFRVAFQGAKDIFNLSPDITIYGKILGGGLPAAALVGHRSILDHLMPEGTIFQAGTMSGNFLAMATGHAAIQL
CQSEGFYDHLSQLEALFYSPIEEEIRSQGFPVSLVHQGTMFSLFFTESAPTNFDEAKNSDVEKFQTFYSEVFDNGVYLSP
SPLEANFISSAHTEENLTYAQNIIIDSLIKIFDSSAQRFF
>Mature_440_residues
MLNCSNQKHTVTFEEACQVFPGGVNSPVRACRSVGVTPPIVSSAQGDIFLDTHGREFIDFCGGWGALIHGHSHPKIVKAI
QKTALKGTSYGLTSEEEILFATMLLSSLKLKEHKIRFVSSGTEATMTAVRLARGITNRSIIIKFIGGYHGHADTLLGGIS
TTEETIDNLTSLIHTPSPHSLLISLPYNNSQILHHVMEALGPQVAGIIFEPICANMGIVLPKAEFLDDIIELCKRFGSLS
IMDEVVTGFRVAFQGAKDIFNLSPDITIYGKILGGGLPAAALVGHRSILDHLMPEGTIFQAGTMSGNFLAMATGHAAIQL
CQSEGFYDHLSQLEALFYSPIEEEIRSQGFPVSLVHQGTMFSLFFTESAPTNFDEAKNSDVEKFQTFYSEVFDNGVYLSP
SPLEANFISSAHTEENLTYAQNIIIDSLIKIFDSSAQRFF

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=306, Percent_Identity=29.0849673202614, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI24119277, Length=325, Percent_Identity=24.9230769230769, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI284507298, Length=226, Percent_Identity=30.0884955752212, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786349, Length=421, Percent_Identity=40.3800475059382, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1787560, Length=314, Percent_Identity=30.5732484076433, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693181, Length=328, Percent_Identity=29.8780487804878, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789016, Length=314, Percent_Identity=27.0700636942675, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789759, Length=333, Percent_Identity=27.6276276276276, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788044, Length=310, Percent_Identity=28.7096774193548, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786991, Length=256, Percent_Identity=28.125, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144271, Length=325, Percent_Identity=25.8461538461538, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32564660, Length=361, Percent_Identity=26.8698060941828, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=161, Percent_Identity=29.8136645962733, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=347, Percent_Identity=26.5129682997118, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324432, Length=326, Percent_Identity=24.5398773006135, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_CHLPN (Q9JRW9)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86508
- PIR:   F81555
- PIR:   G72114
- RefSeq:   NP_224346.1
- RefSeq:   NP_300197.1
- RefSeq:   NP_445176.1
- RefSeq:   NP_876415.1
- ProteinModelPortal:   Q9JRW9
- GeneID:   1466822
- GeneID:   894914
- GeneID:   918926
- GeneID:   963262
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0634
- KEGG:   cpn:CPn0138
- KEGG:   cpt:CpB0139
- TIGR:   CP_0634
- HOGENOM:   HBG725944
- OMA:   HSEADIL
- ProtClustDB:   PRK00615
- BioCyc:   CPNE115711:CP_0634-MONOMER
- BioCyc:   CPNE115713:CPN0138-MONOMER
- BioCyc:   CPNE138677:CPJ0138-MONOMER
- BioCyc:   CPNE182082:CPB0139-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 47956; Mature: 47956

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNCSNQKHTVTFEEACQVFPGGVNSPVRACRSVGVTPPIVSSAQGDIFLDTHGREFIDF
CCCCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHH
CGGWGALIHGHSHPKIVKAIQKTALKGTSYGLTSEEEILFATMLLSSLKLKEHKIRFVSS
HCCCHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEC
GTEATMTAVRLARGITNRSIIIKFIGGYHGHADTLLGGISTTEETIDNLTSLIHTPSPHS
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCE
LLISLPYNNSQILHHVMEALGPQVAGIIFEPICANMGIVLPKAEFLDDIIELCKRFGSLS
EEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCHH
IMDEVVTGFRVAFQGAKDIFNLSPDITIYGKILGGGLPAAALVGHRSILDHLMPEGTIFQ
HHHHHHHHHHHHHHCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
AGTMSGNFLAMATGHAAIQLCQSEGFYDHLSQLEALFYSPIEEEIRSQGFPVSLVHQGTM
CCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCE
FSLFFTESAPTNFDEAKNSDVEKFQTFYSEVFDNGVYLSPSPLEANFISSAHTEENLTYA
EEEEEECCCCCCCHHCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCCHHHH
QNIIIDSLIKIFDSSAQRFF
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNCSNQKHTVTFEEACQVFPGGVNSPVRACRSVGVTPPIVSSAQGDIFLDTHGREFIDF
CCCCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHH
CGGWGALIHGHSHPKIVKAIQKTALKGTSYGLTSEEEILFATMLLSSLKLKEHKIRFVSS
HCCCHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEC
GTEATMTAVRLARGITNRSIIIKFIGGYHGHADTLLGGISTTEETIDNLTSLIHTPSPHS
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCE
LLISLPYNNSQILHHVMEALGPQVAGIIFEPICANMGIVLPKAEFLDDIIELCKRFGSLS
EEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCHH
IMDEVVTGFRVAFQGAKDIFNLSPDITIYGKILGGGLPAAALVGHRSILDHLMPEGTIFQ
HHHHHHHHHHHHHHCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
AGTMSGNFLAMATGHAAIQLCQSEGFYDHLSQLEALFYSPIEEEIRSQGFPVSLVHQGTM
CCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCE
FSLFFTESAPTNFDEAKNSDVEKFQTFYSEVFDNGVYLSPSPLEANFISSAHTEENLTYA
EEEEEECCCCCCCHHCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCCHHHH
QNIIIDSLIKIFDSSAQRFF
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362