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The map label for this gene is metG

Identifier: 15835658

GI number: 15835658

Start: 151782

End: 153437

Strand: Direct

Name: metG

Synonym: CPj0122

Alternate gene names: 15835658

Gene position: 151782-153437 (Clockwise)

Preceding gene: 15835657

Following gene: 15835660

Centisome position: 12.37

GC content: 39.86

Gene sequence:

>1656_bases
ATGCCACAAAAAGTCCTGATTACTTCAGCTTTACCCTATGCTAATGGTCCGCTACATTTTGGACATATTGCAGGAGTCTA
TCTTCCTGCAGATGTGTATGCAAGATTCCGTAGATTGTTAGGAGACGATGTCCTTTATATTTGTGGTTCCGATGAATTTG
GCATAGCGATCACCTTAAATGCGGATCGTGAGGGGTTGGGGTATCAAGAGTACGTGGATATGTACCATAAGTTACATAAA
GATACTTTTGAGAAGTTAGGGTTTGCTTTGGATTTCTTTTCTAGGACGACGAACCCTTTTCATGCTGAGCTTGTCCAAGA
TTTTTATTCCCAACTTAAAGCGTCTGGATTGATTGAAAATCGCATATCTGAACAACTGTATTCAGAACAAGAACAACGTT
TTCTTGCGGATCGTTATGTAGAAGGGACGTGTCCTCGGTGCGGTTTTGATCATGCTCGAGGAGACGAGTGTCAGAGCTGT
GGTGCGGATTATGAGGCTATAGATTTAATCGACCCTAAGTCTAAGATTTCTGGGGTTGAGTTAGTAAAAAAAGAGACTGA
GCACTCATATTTTCTTTTGGACCGTATGAAAGACGCTCTACTTTCTTTTATTCAGGGATGCTATTTACCTGATCATGTCC
GTAAATTTGTTGTTGATTACATAGAACATGTCAGGTCTCGAGCCATTACTCGAGATTTATCTTGGGGGATTCCTGTTCCA
GACTTTCCTGGAAAGGTGTTTTATGTATGGTTTGACGCTCCTATAGGATATATCAGTGGAACTATGGAATGGGCAGCTTC
TCAAGGAAACCCTGACGAATGGAAGCGTTTCTGGCTTGAAGACGGTGTAGAGTATGTCCAGTTTATAGGTAAAGATAATC
TTCCTTTCCATTCTGTAGTTTTCCCAGCTATGGAATTGGGTCAGAAACTTGACTATAAAAAAGTTGATGCCCTCGTAGTT
TCAGAGTTTTATCTTTTAGAAGGACGGCAATTCAGTAAATCCGAGGGCAATTATGTGGATATGGACAAGTTTTTGAGTTC
CTATTCCTTAGACAAATTGCGCTATGTATTGGCGGCTACAGCTCCTGAAACTTCGGATAGTGAGTTTACTTTCCTTGATT
TTAAGACTCGTTGTAATTCTGAGTTGGTAGGAAAGTTTGGGAATTTTATAAACCGAGTTCTTGCTTTTGCAGAAAAGAAT
CACTATGACAAGCTTTCTTATCATTCTGTGGTTTTAGAAGATAGTGACAGGGCATTTCTTGAAGAAGTGCGTCAACTTGT
TCGAGATGCTGAGAAGTGCTACAGAGAGTATAGTTTACGTAAGGCTACGAGTGTGATTATGTCACTGGCAGCTTTAGGGA
ATGTCTATTTTAACCAACAAGCACCTTGGAAGCTATTGAAAGAAGGGACTCGTGAGCGTGTTGAGGCCATTTTATTCTGC
GCATGTTATTGTCAGAAGTTGTTAGCTTTAATTTCTTATCCTATTATTCCCGAAAGCGCTGTAGCTATTTGGGAGATGAT
CTCACCAAAATCTTTAGAAAATTGCAATTTGGATACGATGTATGCTAGGGATCTATGGAAAGAAGAAATTCTTGATGTTA
TAAACGAAGAATTTCATTTGAAGTCCCCCAGGTTATTATTTACTACTGTAGAGTAG

Upstream 100 bases:

>100_bases
TTGTAGTAACTGCTAGCCCTACTGTGGAAAGAAAGAGATCAGAACATACAAATTCTAGAAAAAAAGATCCCTCAGCATAT
ACTTGGAGTGATGTAAAGTA

Downstream 100 bases:

>100_bases
AGCTCGAGGTCTTTTCTTTTTAGAATCCTGATCTGTAGGTGTAATTACAGATCTGCATAATTTTTCTTAGTATCGAGCTC
TTTAAGAACTTCTGCTAGTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MPQKVLITSALPYANGPLHFGHIAGVYLPADVYARFRRLLGDDVLYICGSDEFGIAITLNADREGLGYQEYVDMYHKLHK
DTFEKLGFALDFFSRTTNPFHAELVQDFYSQLKASGLIENRISEQLYSEQEQRFLADRYVEGTCPRCGFDHARGDECQSC
GADYEAIDLIDPKSKISGVELVKKETEHSYFLLDRMKDALLSFIQGCYLPDHVRKFVVDYIEHVRSRAITRDLSWGIPVP
DFPGKVFYVWFDAPIGYISGTMEWAASQGNPDEWKRFWLEDGVEYVQFIGKDNLPFHSVVFPAMELGQKLDYKKVDALVV
SEFYLLEGRQFSKSEGNYVDMDKFLSSYSLDKLRYVLAATAPETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKN
HYDKLSYHSVVLEDSDRAFLEEVRQLVRDAEKCYREYSLRKATSVIMSLAALGNVYFNQQAPWKLLKEGTRERVEAILFC
ACYCQKLLALISYPIIPESAVAIWEMISPKSLENCNLDTMYARDLWKEEILDVINEEFHLKSPRLLFTTVE

Sequences:

>Translated_551_residues
MPQKVLITSALPYANGPLHFGHIAGVYLPADVYARFRRLLGDDVLYICGSDEFGIAITLNADREGLGYQEYVDMYHKLHK
DTFEKLGFALDFFSRTTNPFHAELVQDFYSQLKASGLIENRISEQLYSEQEQRFLADRYVEGTCPRCGFDHARGDECQSC
GADYEAIDLIDPKSKISGVELVKKETEHSYFLLDRMKDALLSFIQGCYLPDHVRKFVVDYIEHVRSRAITRDLSWGIPVP
DFPGKVFYVWFDAPIGYISGTMEWAASQGNPDEWKRFWLEDGVEYVQFIGKDNLPFHSVVFPAMELGQKLDYKKVDALVV
SEFYLLEGRQFSKSEGNYVDMDKFLSSYSLDKLRYVLAATAPETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKN
HYDKLSYHSVVLEDSDRAFLEEVRQLVRDAEKCYREYSLRKATSVIMSLAALGNVYFNQQAPWKLLKEGTRERVEAILFC
ACYCQKLLALISYPIIPESAVAIWEMISPKSLENCNLDTMYARDLWKEEILDVINEEFHLKSPRLLFTTVE
>Mature_550_residues
PQKVLITSALPYANGPLHFGHIAGVYLPADVYARFRRLLGDDVLYICGSDEFGIAITLNADREGLGYQEYVDMYHKLHKD
TFEKLGFALDFFSRTTNPFHAELVQDFYSQLKASGLIENRISEQLYSEQEQRFLADRYVEGTCPRCGFDHARGDECQSCG
ADYEAIDLIDPKSKISGVELVKKETEHSYFLLDRMKDALLSFIQGCYLPDHVRKFVVDYIEHVRSRAITRDLSWGIPVPD
FPGKVFYVWFDAPIGYISGTMEWAASQGNPDEWKRFWLEDGVEYVQFIGKDNLPFHSVVFPAMELGQKLDYKKVDALVVS
EFYLLEGRQFSKSEGNYVDMDKFLSSYSLDKLRYVLAATAPETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKNH
YDKLSYHSVVLEDSDRAFLEEVRQLVRDAEKCYREYSLRKATSVIMSLAALGNVYFNQQAPWKLLKEGTRERVEAILFCA
CYCQKLLALISYPIIPESAVAIWEMISPKSLENCNLDTMYARDLWKEEILDVINEEFHLKSPRLLFTTVE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=514, Percent_Identity=37.15953307393, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI24308436, Length=523, Percent_Identity=26.0038240917782, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788432, Length=503, Percent_Identity=32.20675944334, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1786861, Length=148, Percent_Identity=30.4054054054054, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540794, Length=521, Percent_Identity=35.3166986564299, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI212645096, Length=508, Percent_Identity=25.3937007874016, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71992781, Length=338, Percent_Identity=25.4437869822485, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321704, Length=527, Percent_Identity=33.7760910815939, Blast_Score=312, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24655311, Length=523, Percent_Identity=35.5640535372849, Blast_Score=311, Evalue=9e-85,
Organism=Drosophila melanogaster, GI21358483, Length=521, Percent_Identity=26.295585412668, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CHLPN (Q9Z959)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86506
- PIR:   H72117
- PIR:   H81552
- RefSeq:   NP_224330.1
- RefSeq:   NP_300182.1
- RefSeq:   NP_445193.1
- RefSeq:   NP_876399.1
- ProteinModelPortal:   Q9Z959
- PHCI-2DPAGE:   Q9Z959
- GeneID:   1466806
- GeneID:   894667
- GeneID:   918910
- GeneID:   963233
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0651
- KEGG:   cpn:CPn0122
- KEGG:   cpt:CpB0123
- TIGR:   CP_0651
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- PhylomeDB:   Q9Z959
- ProtClustDB:   PRK12268
- BioCyc:   CPNE115711:CP_0651-MONOMER
- BioCyc:   CPNE115713:CPN0122-MONOMER
- BioCyc:   CPNE138677:CPJ0122-MONOMER
- BioCyc:   CPNE182082:CPB0123-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 63454; Mature: 63323

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQKVLITSALPYANGPLHFGHIAGVYLPADVYARFRRLLGDDVLYICGSDEFGIAITLN
CCCCEEEEECCCCCCCCEECHHHCCEEECHHHHHHHHHHCCCCEEEEECCCCCCEEEEEE
ADREGLGYQEYVDMYHKLHKDTFEKLGFALDFFSRTTNPFHAELVQDFYSQLKASGLIEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RISEQLYSEQEQRFLADRYVEGTCPRCGFDHARGDECQSCGADYEAIDLIDPKSKISGVE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCEEECCCCHHHHHHHH
LVKKETEHSYFLLDRMKDALLSFIQGCYLPDHVRKFVVDYIEHVRSRAITRDLSWGIPVP
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DFPGKVFYVWFDAPIGYISGTMEWAASQGNPDEWKRFWLEDGVEYVQFIGKDNLPFHSVV
CCCCCEEEEEECCCHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FPAMELGQKLDYKKVDALVVSEFYLLEGRQFSKSEGNYVDMDKFLSSYSLDKLRYVLAAT
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCC
APETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKNHYDKLSYHSVVLEDSDRAFL
CCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCHHHH
EEVRQLVRDAEKCYREYSLRKATSVIMSLAALGNVYFNQQAPWKLLKEGTRERVEAILFC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
ACYCQKLLALISYPIIPESAVAIWEMISPKSLENCNLDTMYARDLWKEEILDVINEEFHL
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHCC
KSPRLLFTTVE
CCCCEEEEECC
>Mature Secondary Structure 
PQKVLITSALPYANGPLHFGHIAGVYLPADVYARFRRLLGDDVLYICGSDEFGIAITLN
CCCEEEEECCCCCCCCEECHHHCCEEECHHHHHHHHHHCCCCEEEEECCCCCCEEEEEE
ADREGLGYQEYVDMYHKLHKDTFEKLGFALDFFSRTTNPFHAELVQDFYSQLKASGLIEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RISEQLYSEQEQRFLADRYVEGTCPRCGFDHARGDECQSCGADYEAIDLIDPKSKISGVE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCEEECCCCHHHHHHHH
LVKKETEHSYFLLDRMKDALLSFIQGCYLPDHVRKFVVDYIEHVRSRAITRDLSWGIPVP
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DFPGKVFYVWFDAPIGYISGTMEWAASQGNPDEWKRFWLEDGVEYVQFIGKDNLPFHSVV
CCCCCEEEEEECCCHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FPAMELGQKLDYKKVDALVVSEFYLLEGRQFSKSEGNYVDMDKFLSSYSLDKLRYVLAAT
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCC
APETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKNHYDKLSYHSVVLEDSDRAFL
CCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCHHHH
EEVRQLVRDAEKCYREYSLRKATSVIMSLAALGNVYFNQQAPWKLLKEGTRERVEAILFC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
ACYCQKLLALISYPIIPESAVAIWEMISPKSLENCNLDTMYARDLWKEEILDVINEEFHL
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHCC
KSPRLLFTTVE
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362