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The map label for this gene is trmD

Identifier: 15835653

GI number: 15835653

Start: 148700

End: 149785

Strand: Direct

Name: trmD

Synonym: CPj0117

Alternate gene names: 15835653

Gene position: 148700-149785 (Clockwise)

Preceding gene: 15835652

Following gene: 15835654

Centisome position: 12.12

GC content: 41.25

Gene sequence:

>1086_bases
ATGAAGATCGATATACTTTCTTTATTCCCAGGTTATTTTGATGGTCCATTGCAAACGAGTATTCTTGGTAGGGCCATAAA
GCAGAGACTCTTAGATGTCCAGCTTACAAATCTTCGTGACTTTGGACTCGGAAAGTGGAAACAAGTTGATGATACTCCGT
TTAGTGGTGGTGGGATGCTTTTAATGGCAGAGCCTGTCACTTCAGCTATTAGGAGTGTAAGAAAGGAGAATTCTAAGGTA
ATTTACCTCTCTCCTCAAGGAGCTTTGTTGACAGCTGAAAAGAGTCGAGAATTGGCTGCTGCTTCGCATTTGATATTACT
TTGCGGTCACTACGAAGGTATTGATGAGCGTGCTATAGAGAGCGAAGTGGATGAAGAGATTAGTATAGGGGACTATGTCC
TGACTAATGGTGGAATTGCTGCTCTGGTCCTTATCGATGCAGTTTCTCGTTTTATTCCCGGTGTATTGGGGAATCAAGAG
AGTGCTGAGAGAGATTCTTTAGAAAATGGTTTGCTAGAAGGACCTCAGTATACACGCCCTAGAGAGTTTGAAGGGAAAGA
AGTTCCAGAAGTATTGTTGCAAGGGGATCACAAAGCCATTTCTCAGTGGAGATTGGAGCAAAGTGAGCGTAGAACTTATG
AGAGACGTCCTGATTTGTATCTGAACTATCTCTATAAACGCTCGATTGATCACAAATTTGATGAGGAGACTACAACAAAT
AGGGATCATTTCAAGTGTGACAAGATTTCTGTAGTACTAGAGGTAAATAAGTTAAAGCGCGCAAAAAATTTTTACTGTAA
GGTATTCGGTCTGGATGCCATGAGCTGCGAGAATAAATTTTGTCTTCCTCATGAAGGCAAAACCATATTCTGGTTACGAG
AAGTTCAAGCTGAGAAAAAAAACATAGTGACTCTCTCCCTTTCCTTAGATTGTGCATGCGAAGAGGACTTTTGTTATCTT
CTTAGAAGATGGGAGTTATTTGGTGGAAAGTTGTTAGAAAAGCAAGCTGATGAGCATGCTGTATGGGCCCTAGCACAAGA
TTTAGATGGGCATGCATGGATATTCTCGTGGCATAGGATGAAATAG

Upstream 100 bases:

>100_bases
AAGTTAGTTGTTCGTAAGAAGCGACGTGCTTATAGACAGCGTCGGTCTACACAAAGAGAAGAGGCTGCAAAAGATGCAAC
TAAGTAGGTAGTGAACTGGG

Downstream 100 bases:

>100_bases
AAGAAAGAGAATTTTAGGTGGTATATTATGGTGAATTTACTCAAAGAATTAGAACAAGAACAGTGTAGGAATGATCTTCC
CGAGTTTCATGTTGGCGATA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKIDILSLFPGYFDGPLQTSILGRAIKQRLLDVQLTNLRDFGLGKWKQVDDTPFSGGGMLLMAEPVTSAIRSVRKENSKV
IYLSPQGALLTAEKSRELAAASHLILLCGHYEGIDERAIESEVDEEISIGDYVLTNGGIAALVLIDAVSRFIPGVLGNQE
SAERDSLENGLLEGPQYTRPREFEGKEVPEVLLQGDHKAISQWRLEQSERRTYERRPDLYLNYLYKRSIDHKFDEETTTN
RDHFKCDKISVVLEVNKLKRAKNFYCKVFGLDAMSCENKFCLPHEGKTIFWLREVQAEKKNIVTLSLSLDCACEEDFCYL
LRRWELFGGKLLEKQADEHAVWALAQDLDGHAWIFSWHRMK

Sequences:

>Translated_361_residues
MKIDILSLFPGYFDGPLQTSILGRAIKQRLLDVQLTNLRDFGLGKWKQVDDTPFSGGGMLLMAEPVTSAIRSVRKENSKV
IYLSPQGALLTAEKSRELAAASHLILLCGHYEGIDERAIESEVDEEISIGDYVLTNGGIAALVLIDAVSRFIPGVLGNQE
SAERDSLENGLLEGPQYTRPREFEGKEVPEVLLQGDHKAISQWRLEQSERRTYERRPDLYLNYLYKRSIDHKFDEETTTN
RDHFKCDKISVVLEVNKLKRAKNFYCKVFGLDAMSCENKFCLPHEGKTIFWLREVQAEKKNIVTLSLSLDCACEEDFCYL
LRRWELFGGKLLEKQADEHAVWALAQDLDGHAWIFSWHRMK
>Mature_361_residues
MKIDILSLFPGYFDGPLQTSILGRAIKQRLLDVQLTNLRDFGLGKWKQVDDTPFSGGGMLLMAEPVTSAIRSVRKENSKV
IYLSPQGALLTAEKSRELAAASHLILLCGHYEGIDERAIESEVDEEISIGDYVLTNGGIAALVLIDAVSRFIPGVLGNQE
SAERDSLENGLLEGPQYTRPREFEGKEVPEVLLQGDHKAISQWRLEQSERRTYERRPDLYLNYLYKRSIDHKFDEETTTN
RDHFKCDKISVVLEVNKLKRAKNFYCKVFGLDAMSCENKFCLPHEGKTIFWLREVQAEKKNIVTLSLSLDCACEEDFCYL
LRRWELFGGKLLEKQADEHAVWALAQDLDGHAWIFSWHRMK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=226, Percent_Identity=52.212389380531, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CHLPN (Q9Z964)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C72117
- PIR:   F86505
- RefSeq:   NP_224325.1
- RefSeq:   NP_300177.1
- RefSeq:   NP_445198.1
- RefSeq:   NP_876394.1
- ProteinModelPortal:   Q9Z964
- SMR:   Q9Z964
- GeneID:   1466801
- GeneID:   894638
- GeneID:   918905
- GeneID:   963709
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0656
- KEGG:   cpn:CPn0117
- KEGG:   cpt:CpB0118
- TIGR:   CP_0656
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- PhylomeDB:   Q9Z964
- ProtClustDB:   PRK01037
- BioCyc:   CPNE115711:CP_0656-MONOMER
- BioCyc:   CPNE115713:CPN0117-MONOMER
- BioCyc:   CPNE138677:CPJ0117-MONOMER
- BioCyc:   CPNE182082:CPB0118-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 41249; Mature: 41249

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDILSLFPGYFDGPLQTSILGRAIKQRLLDVQLTNLRDFGLGKWKQVDDTPFSGGGML
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCEE
LMAEPVTSAIRSVRKENSKVIYLSPQGALLTAEKSRELAAASHLILLCGHYEGIDERAIE
EEECHHHHHHHHHHHCCCEEEEECCCCCEEECHHHHHHHHHHEEEEEEECCCCCHHHHHH
SEVDEEISIGDYVLTNGGIAALVLIDAVSRFIPGVLGNQESAERDSLENGLLEGPQYTRP
HHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
REFEGKEVPEVLLQGDHKAISQWRLEQSERRTYERRPDLYLNYLYKRSIDHKFDEETTTN
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC
RDHFKCDKISVVLEVNKLKRAKNFYCKVFGLDAMSCENKFCLPHEGKTIFWLREVQAEKK
CCCEEECEEEEEEEEHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHCCC
NIVTLSLSLDCACEEDFCYLLRRWELFGGKLLEKQADEHAVWALAQDLDGHAWIFSWHRM
CEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCCEEEEEEECC
K
C
>Mature Secondary Structure
MKIDILSLFPGYFDGPLQTSILGRAIKQRLLDVQLTNLRDFGLGKWKQVDDTPFSGGGML
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCEE
LMAEPVTSAIRSVRKENSKVIYLSPQGALLTAEKSRELAAASHLILLCGHYEGIDERAIE
EEECHHHHHHHHHHHCCCEEEEECCCCCEEECHHHHHHHHHHEEEEEEECCCCCHHHHHH
SEVDEEISIGDYVLTNGGIAALVLIDAVSRFIPGVLGNQESAERDSLENGLLEGPQYTRP
HHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
REFEGKEVPEVLLQGDHKAISQWRLEQSERRTYERRPDLYLNYLYKRSIDHKFDEETTTN
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC
RDHFKCDKISVVLEVNKLKRAKNFYCKVFGLDAMSCENKFCLPHEGKTIFWLREVQAEKK
CCCEEECEEEEEEEEHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHCCC
NIVTLSLSLDCACEEDFCYLLRRWELFGGKLLEKQADEHAVWALAQDLDGHAWIFSWHRM
CEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCCEEEEEEECC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362