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The map label for this gene is prfA

Identifier: 15835649

GI number: 15835649

Start: 145046

End: 146119

Strand: Direct

Name: prfA

Synonym: CPj0113

Alternate gene names: 15835649

Gene position: 145046-146119 (Clockwise)

Preceding gene: 15835648

Following gene: 15835650

Centisome position: 11.83

GC content: 40.69

Gene sequence:

>1074_bases
ATGAAGAAAAAAGTTGCCGAGTATTTAAACCGTTTAGCAGAAGTCGAAATAAAAATTTCAAATCCTGAAATTTTTTCTAA
TTCTAAAGAATATAGCGCTCTTAGCAAGGAACATTCTTATCTTCTAGAATTGAAAAACGCCTACGATAAAATCTTAAATT
TAGAAAAAGTCCTTGCTGATGATAAGCAAGCTTTAGCTATTGAGAAAGATCCAGAGATGGTCGTTATGCTTGAAGAGGGG
ATTAACGAAAATAAAGTAGAGCTAGAGAAATTAAATAAAATATTAGAAAGCTTATTAGTCCCCCCAGATCCTGATGATGA
TCTAAATGTCATTATGGAACTACGAGCCGGTACAGGAGGCGAGGAAGCCGCTCTCTTTGTTGGAGATTGTGTCCGCATGT
ATCACCTGTACGCCTCCTCTAAGGGATGGAAATACGAGGTACTCTCTGCGTCAGAATCCGATCTTAAGGGATATAAGGAA
TACGTCATGGGGATCTCAGGAACTGGGGTGAAGCGTTTACTTCAGTATGAGGCTGGTACACATCGAGTTCAGAGAGTTCC
TGAAACAGAAACTCAAGGACGTGTACATACATCTGCAATTACAATCGCTGTCCTTCCAGAACCTTCAGAAGAAGATACAG
AGCTTCTTATTAATGAGAAGGATTTAAAAATTGATACATTCAGAGCCTCTGGTGCTGGAGGACAGCACGTAAACGTTACT
GATTCTGCGGTGAGAATCACACACCTGCCTACAGGTGTTGTAGTTACATGCCAGGATGAGCGCAGTCAACATAAAAATAA
AGATAAGGCCATGCGGATTCTTAAAGCCCGGATTCGTGATGCAGAAATGCAAAAACGCCATAACGAGGCGTCTGCTATGC
GTTCTGCTCAGGTAGGAAGTGGGGATCGTTCCGAGAGAATTCGCACCTATAATTTTTCTCAAAATCGCGTGACTGATCAT
AGAATCGGATTAACTTTATATAACTTAGATAAAGTTATGGAAGGAGACCTAGATCCAATTACGACTGCAATGGTGAGTCA
TGCCTACCACCAGTTACTCGAACATGGAAATTAA

Upstream 100 bases:

>100_bases
TAGATAACGTTAGATGCCTGTATCAGTATACCAAGGTGTTTTAGAGAATTTTTTATCTGATTCCATTTGATACTGATTTT
TCACCACTTTTTAGGGATTC

Downstream 100 bases:

>100_bases
AAAGGCGATTCAAGAGGGAACCGCTTACCTAGATTATTATGGGGTGCCTCTTTCTGATTGCGAAGCCCTGTATATTCTCA
TGGATTTATTAGAAGTCAGT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKKKVAEYLNRLAEVEIKISNPEIFSNSKEYSALSKEHSYLLELKNAYDKILNLEKVLADDKQALAIEKDPEMVVMLEEG
INENKVELEKLNKILESLLVPPDPDDDLNVIMELRAGTGGEEAALFVGDCVRMYHLYASSKGWKYEVLSASESDLKGYKE
YVMGISGTGVKRLLQYEAGTHRVQRVPETETQGRVHTSAITIAVLPEPSEEDTELLINEKDLKIDTFRASGAGGQHVNVT
DSAVRITHLPTGVVVTCQDERSQHKNKDKAMRILKARIRDAEMQKRHNEASAMRSAQVGSGDRSERIRTYNFSQNRVTDH
RIGLTLYNLDKVMEGDLDPITTAMVSHAYHQLLEHGN

Sequences:

>Translated_357_residues
MKKKVAEYLNRLAEVEIKISNPEIFSNSKEYSALSKEHSYLLELKNAYDKILNLEKVLADDKQALAIEKDPEMVVMLEEG
INENKVELEKLNKILESLLVPPDPDDDLNVIMELRAGTGGEEAALFVGDCVRMYHLYASSKGWKYEVLSASESDLKGYKE
YVMGISGTGVKRLLQYEAGTHRVQRVPETETQGRVHTSAITIAVLPEPSEEDTELLINEKDLKIDTFRASGAGGQHVNVT
DSAVRITHLPTGVVVTCQDERSQHKNKDKAMRILKARIRDAEMQKRHNEASAMRSAQVGSGDRSERIRTYNFSQNRVTDH
RIGLTLYNLDKVMEGDLDPITTAMVSHAYHQLLEHGN
>Mature_357_residues
MKKKVAEYLNRLAEVEIKISNPEIFSNSKEYSALSKEHSYLLELKNAYDKILNLEKVLADDKQALAIEKDPEMVVMLEEG
INENKVELEKLNKILESLLVPPDPDDDLNVIMELRAGTGGEEAALFVGDCVRMYHLYASSKGWKYEVLSASESDLKGYKE
YVMGISGTGVKRLLQYEAGTHRVQRVPETETQGRVHTSAITIAVLPEPSEEDTELLINEKDLKIDTFRASGAGGQHVNVT
DSAVRITHLPTGVVVTCQDERSQHKNKDKAMRILKARIRDAEMQKRHNEASAMRSAQVGSGDRSERIRTYNFSQNRVTDH
RIGLTLYNLDKVMEGDLDPITTAMVSHAYHQLLEHGN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=311, Percent_Identity=46.3022508038585, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI34577120, Length=333, Percent_Identity=39.3393393393393, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI166795305, Length=205, Percent_Identity=45.3658536585366, Blast_Score=174, Evalue=8e-44,
Organism=Escherichia coli, GI1787462, Length=344, Percent_Identity=52.3255813953488, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=38.0530973451327, Blast_Score=206, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=331, Percent_Identity=35.6495468277946, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=324, Percent_Identity=40.4320987654321, Blast_Score=236, Evalue=3e-63,
Organism=Drosophila melanogaster, GI19921226, Length=314, Percent_Identity=43.9490445859873, Blast_Score=241, Evalue=7e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CHLPN (Q9Z968)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86505
- PIR:   G72116
- RefSeq:   NP_224321.1
- RefSeq:   NP_300173.1
- RefSeq:   NP_445202.1
- RefSeq:   NP_876390.1
- ProteinModelPortal:   Q9Z968
- SMR:   Q9Z968
- GeneID:   1466797
- GeneID:   895574
- GeneID:   918901
- GeneID:   963234
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0660
- KEGG:   cpn:CPn0113
- KEGG:   cpt:CpB0114
- TIGR:   CP_0660
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q9Z968
- ProtClustDB:   PRK00591
- BioCyc:   CPNE115711:CP_0660-MONOMER
- BioCyc:   CPNE115713:CPN0113-MONOMER
- BioCyc:   CPNE138677:CPJ0113-MONOMER
- BioCyc:   CPNE182082:CPB0114-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40151; Mature: 40151

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVAEYLNRLAEVEIKISNPEIFSNSKEYSALSKEHSYLLELKNAYDKILNLEKVLAD
CCHHHHHHHHHHHEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKQALAIEKDPEMVVMLEEGINENKVELEKLNKILESLLVPPDPDDDLNVIMELRAGTGG
CCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEECCCCC
EEAALFVGDCVRMYHLYASSKGWKYEVLSASESDLKGYKEYVMGISGTGVKRLLQYEAGT
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
HRVQRVPETETQGRVHTSAITIAVLPEPSEEDTELLINEKDLKIDTFRASGAGGQHVNVT
HHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCEEEEC
DSAVRITHLPTGVVVTCQDERSQHKNKDKAMRILKARIRDAEMQKRHNEASAMRSAQVGS
CCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDRSERIRTYNFSQNRVTDHRIGLTLYNLDKVMEGDLDPITTAMVSHAYHQLLEHGN
CCCCCCEEEEECCCCCCCHHEEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKVAEYLNRLAEVEIKISNPEIFSNSKEYSALSKEHSYLLELKNAYDKILNLEKVLAD
CCHHHHHHHHHHHEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKQALAIEKDPEMVVMLEEGINENKVELEKLNKILESLLVPPDPDDDLNVIMELRAGTGG
CCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEECCCCC
EEAALFVGDCVRMYHLYASSKGWKYEVLSASESDLKGYKEYVMGISGTGVKRLLQYEAGT
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
HRVQRVPETETQGRVHTSAITIAVLPEPSEEDTELLINEKDLKIDTFRASGAGGQHVNVT
HHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCEEEEC
DSAVRITHLPTGVVVTCQDERSQHKNKDKAMRILKARIRDAEMQKRHNEASAMRSAQVGS
CCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDRSERIRTYNFSQNRVTDHRIGLTLYNLDKVMEGDLDPITTAMVSHAYHQLLEHGN
CCCCCCEEEEECCCCCCCHHEEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362