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The map label for this gene is nusG

Identifier: 15835611

GI number: 15835611

Start: 91028

End: 91576

Strand: Direct

Name: nusG

Synonym: CPj0076

Alternate gene names: 15835611

Gene position: 91028-91576 (Clockwise)

Preceding gene: 15835610

Following gene: 15835612

Centisome position: 7.42

GC content: 38.25

Gene sequence:

>549_bases
ATGTATAAATGGTATGTCGTTCAAGTTTTTACAGCTCAAGAAAAGAAAGTAAAAAAGGCTTTAGAAGATTTTAAAGAGTC
TTCAGGAATGACTGATTTTATACAGGAAATTATCTTGCCTATTGAAAATGTCATGGAAGTGAAAAAAGGAGAACATAAGG
TCGTTGAAAAATACATCTGGCCTGGATACCTCTTAGTTAAAATGCATCTGACTGACGAGTCTTGGCTCTATGTTAAAAGT
ACAGCAGGTATAGTCGAGTTTCTTGGAGGCGGAGTCCCTGTAGCTCTTTCTGAAGATGAAGTAAGAAGTATCTTAACAGA
TATAGAAGAGAAGAAATCGGGAGTGGTGCAAAAACATCAGTTCGAGGTTGGTTCTAGAGTGAAAATTAATGACGGAGTCT
TTGTCAATTTTATCGGCACGGTTTCCGAAGTTTTCCATGATAAAGGACGCCTGAGTGTTATGGTTTCTATCTTTGGAAGA
GAAACTAGGGTAGATGATTTAGAATTTTGGCAAGTGGAAGAGGTAGCCCCAGGGCAAGAAAGTGAGTAG

Upstream 100 bases:

>100_bases
ATTTTTGGTTTTGGATTTGCTATTTATTTCGTAGATCTTGTGTTGCGTAAGTCAATCACATGTTTAGATGGTATAACAAC
CTTTTTGTTCGGTTAATTGC

Downstream 100 bases:

>100_bases
ATAGGTTAAAATCAGTGTATTCTTATTCCTTATCTTCTTATAATTTTAGTTTTTCGTTTCTTACCCTCTGTTTGTAGAGG
TGTCTCAGTGCAAAGTAAGG

Product: transcription antitermination protein NusG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MYKWYVVQVFTAQEKKVKKALEDFKESSGMTDFIQEIILPIENVMEVKKGEHKVVEKYIWPGYLLVKMHLTDESWLYVKS
TAGIVEFLGGGVPVALSEDEVRSILTDIEEKKSGVVQKHQFEVGSRVKINDGVFVNFIGTVSEVFHDKGRLSVMVSIFGR
ETRVDDLEFWQVEEVAPGQESE

Sequences:

>Translated_182_residues
MYKWYVVQVFTAQEKKVKKALEDFKESSGMTDFIQEIILPIENVMEVKKGEHKVVEKYIWPGYLLVKMHLTDESWLYVKS
TAGIVEFLGGGVPVALSEDEVRSILTDIEEKKSGVVQKHQFEVGSRVKINDGVFVNFIGTVSEVFHDKGRLSVMVSIFGR
ETRVDDLEFWQVEEVAPGQESE
>Mature_182_residues
MYKWYVVQVFTAQEKKVKKALEDFKESSGMTDFIQEIILPIENVMEVKKGEHKVVEKYIWPGYLLVKMHLTDESWLYVKS
TAGIVEFLGGGVPVALSEDEVRSILTDIEEKKSGVVQKHQFEVGSRVKINDGVFVNFIGTVSEVFHDKGRLSVMVSIFGR
ETRVDDLEFWQVEEVAPGQESE

Specific function: Influences transcription termination and antitermination. Acts as a component of the transcription complex, and interacts with the termination factor rho and RNA polymerase

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KOW domain

Homologues:

Organism=Escherichia coli, GI1790414, Length=175, Percent_Identity=38.8571428571429, Blast_Score=127, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUSG_CHLPN (Q9Z9A5)

Other databases:

- EMBL:   AE001363
- EMBL:   BA000008
- EMBL:   AE002161
- EMBL:   AE009440
- PIR:   C81547
- PIR:   D86500
- PIR:   H72123
- RefSeq:   NP_224284.1
- RefSeq:   NP_300135.1
- RefSeq:   NP_445241.1
- RefSeq:   NP_876352.1
- ProteinModelPortal:   Q9Z9A5
- SMR:   Q9Z9A5
- PHCI-2DPAGE:   Q9Z9A5
- GeneID:   1466759
- GeneID:   894692
- GeneID:   918867
- GeneID:   963204
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0699
- KEGG:   cpn:CPn0076
- KEGG:   cpt:CpB0076
- TIGR:   CP_0699
- HOGENOM:   HBG751162
- OMA:   VASGCEK
- ProtClustDB:   PRK05609
- BioCyc:   CPNE115711:CP_0699-MONOMER
- BioCyc:   CPNE115713:CPN0076-MONOMER
- BioCyc:   CPNE138677:CPJ0076-MONOMER
- BioCyc:   CPNE182082:CPB0076-MONOMER
- InterPro:   IPR005824
- InterPro:   IPR001062
- InterPro:   IPR006645
- InterPro:   IPR014722
- InterPro:   IPR008991
- Gene3D:   G3DSA:2.30.30.30
- Gene3D:   G3DSA:3.30.70.940
- PRINTS:   PR00338
- SMART:   SM00739
- SMART:   SM00738
- TIGRFAMs:   TIGR00922

Pfam domain/function: PF02357 NusG; SSF82679 Transcrpt_antiterm_NusG_N; SSF50104 Transl_SH3_like

EC number: NA

Molecular weight: Translated: 20792; Mature: 20792

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01014 NUSG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKWYVVQVFTAQEKKVKKALEDFKESSGMTDFIQEIILPIENVMEVKKGEHKVVEKYIW
CCEEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
PGYLLVKMHLTDESWLYVKSTAGIVEFLGGGVPVALSEDEVRSILTDIEEKKSGVVQKHQ
CCEEEEEEEECCCCEEEEECHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCHHHHH
FEVGSRVKINDGVFVNFIGTVSEVFHDKGRLSVMVSIFGRETRVDDLEFWQVEEVAPGQE
CCCCCEEEECCCEEEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHEEECCCCCCCC
SE
CC
>Mature Secondary Structure
MYKWYVVQVFTAQEKKVKKALEDFKESSGMTDFIQEIILPIENVMEVKKGEHKVVEKYIW
CCEEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
PGYLLVKMHLTDESWLYVKSTAGIVEFLGGGVPVALSEDEVRSILTDIEEKKSGVVQKHQ
CCEEEEEEEECCCCEEEEECHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCHHHHH
FEVGSRVKINDGVFVNFIGTVSEVFHDKGRLSVMVSIFGRETRVDDLEFWQVEEVAPGQE
CCCCCEEEECCCEEEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHEEECCCCCCCC
SE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10871362; 10684935