BacMap

Definition	Chlamydophila pneumoniae J138, complete genome.
Accession	NC_002491
Length	1,226,565

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The map label for this gene is tuf

Identifier: 15835609

GI number: 15835609

Start: 89444

End: 90628

Strand: Direct

Name: tuf

Synonym: CPj0074

Alternate gene names: 15835609

Gene position: 89444-90628 (Clockwise)

Preceding gene: 15835608

Following gene: 15835610

Centisome position: 7.29

GC content: 43.12

Gene sequence:

>1185_bases
ATGTCAAAAGAAACTTTTCAACGTAATAAGCCCCATATCAATATTGGGACGATCGGGCACGTTGACCATGGTAAAACTAC
GCTAACAGCGGCAATTACACGCGCGCTATCAGGGGATGGATTGGCGTCTTTCCGTGACTATAGTTCAATTGACAATACTC
CAGAAGAAAAGGCTCGTGGAATTACTATCAACGCTTCTCACGTTGAATACGAAACCCCAAATCGTCACTACGCTCACGTA
GACTGCCCTGGTCACGCTGACTATGTTAAAAATATGATTACAGGCGCCGCTCAAATGGACGGAGCTATCCTAGTCGTTTC
AGCTACAGACGGAGCTATGCCACAAACTAAAGAACATATCTTGCTAGCTCGCCAGGTTGGAGTTCCTTATATCGTTGTTT
TCTTGAATAAAGTAGATATGATCTCTCAAGAAGATGCTGAACTTATTGACCTTGTTGAGATGGAACTTAGTGAGCTTCTT
GAAGAAAAAGGCTACAAAGGATGCCCTATTATCCGTGGTTCTGCTTTGAAAGCTCTTGAAGGTGATGCAAATTATATCGA
AAAAGTTCGAGAACTTATGCAAGCTGTGGATGACAACATCCCTACACCAGAAAGAGAAATTGATAAGCCTTTCTTAATGC
CTATCGAAGACGTATTCTCAATCTCTGGTCGTGGTACTGTGGTTACAGGAAGAATCGAGCGTGGAATCGTTAAAGTTTCT
GATAAAGTTCAGCTCGTGGGATTAGGAGAGACTAAAGAAACAATCGTTACTGGAGTCGAAATGTTCAGGAAAGAACTTCC
TGAAGGTCGTGCAGGAGAAAACGTTGGTTTACTCCTCAGAGGTATTGGAAAGAACGATGTTGAAAGAGGTATGGTGGTTT
GTCAGCCTAACAGCGTGAAGCCTCATACGAAATTTAAGTCAGCTGTTTACGTTCTTCAGAAAGAAGAAGGCGGACGTCAT
AAGCCTTTCTTCAGCGGATACAGACCTCAGTTCTTCTTCCGTACTACAGACGTGACAGGAGTCGTAACTCTTCCTGAAGG
AACTGAAATGGTAATGCCTGGAGATAACGTTGAGCTTGATGTTGAGCTCATTGGAACAGTTGCTCTTGAAGAAGGAATGA
GATTTGCAATTCGTGAAGGTGGTCGTACTATCGGCGCTGGAACGATTTCAAAGATCAATGCTTAA

Upstream 100 bases:

>100_bases
GTAGAGCACTTGCATGGTAAGCAAGCGGTCGTAGGTTCAATTCCTATTCTGGGCAGAAAGAATGGTTGGAGTAATCAATA
ATTTTTAAGAGGATTTTGAG

Downstream 100 bases:

>100_bases
AAATGAATTTCGCGATGATTTTCATCATCGCGATTTTCTGGGTGTGTAGCTTAGCTGGTAGAGCAGTGGCCTCCAAAGCC
GCCGGTCGGGGGTTCGATTC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLASFRDYSSIDNTPEEKARGITINASHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKVDMISQEDAELIDLVEMELSELL
EEKGYKGCPIIRGSALKALEGDANYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVS
DKVQLVGLGETKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCQPNSVKPHTKFKSAVYVLQKEEGGRH
KPFFSGYRPQFFFRTTDVTGVVTLPEGTEMVMPGDNVELDVELIGTVALEEGMRFAIREGGRTIGAGTISKINA

Sequences:

>Translated_394_residues
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLASFRDYSSIDNTPEEKARGITINASHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKVDMISQEDAELIDLVEMELSELL
EEKGYKGCPIIRGSALKALEGDANYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVS
DKVQLVGLGETKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCQPNSVKPHTKFKSAVYVLQKEEGGRH
KPFFSGYRPQFFFRTTDVTGVVTLPEGTEMVMPGDNVELDVELIGTVALEEGMRFAIREGGRTIGAGTISKINA
>Mature_393_residues
SKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLASFRDYSSIDNTPEEKARGITINASHVEYETPNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKVDMISQEDAELIDLVEMELSELLE
EKGYKGCPIIRGSALKALEGDANYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVSD
KVQLVGLGETKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCQPNSVKPHTKFKSAVYVLQKEEGGRHK
PFFSGYRPQFFFRTTDVTGVVTLPEGTEMVMPGDNVELDVELIGTVALEEGMRFAIREGGRTIGAGTISKINA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=55.1637279596977, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=28.7981859410431, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=28.8636363636364, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503475, Length=353, Percent_Identity=32.5779036827195, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=29.6213808463252, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI54607086, Length=330, Percent_Identity=28.7878787878788, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=26.9585253456221, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=26.9585253456221, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=26.9585253456221, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI46094014, Length=434, Percent_Identity=27.1889400921659, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI82546879, Length=426, Percent_Identity=24.8826291079812, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4503507, Length=299, Percent_Identity=27.7591973244147, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI94966754, Length=146, Percent_Identity=35.6164383561644, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI157426893, Length=305, Percent_Identity=26.8852459016393, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI310132016, Length=121, Percent_Identity=35.5371900826446, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310110807, Length=121, Percent_Identity=35.5371900826446, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310123363, Length=121, Percent_Identity=35.5371900826446, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=68.7817258883249, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=68.7817258883249, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI2367247, Length=335, Percent_Identity=31.3432835820896, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994988, Length=299, Percent_Identity=32.4414715719064, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789108, Length=343, Percent_Identity=27.1137026239067, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=270, Percent_Identity=27.4074074074074, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=51.75, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.3298969072165, Blast_Score=333, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=339, Percent_Identity=33.0383480825959, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=339, Percent_Identity=33.0383480825959, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=310, Percent_Identity=32.9032258064516, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=444, Percent_Identity=27.2522522522523, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=444, Percent_Identity=27.2522522522523, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=436, Percent_Identity=26.605504587156, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=27.2425249169435, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=37.4149659863946, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=269, Percent_Identity=30.1115241635688, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17565438, Length=426, Percent_Identity=24.8826291079812, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=290, Percent_Identity=26.551724137931, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=62.1212121212121, Blast_Score=498, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6325337, Length=352, Percent_Identity=32.3863636363636, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=352, Percent_Identity=32.3863636363636, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6320377, Length=353, Percent_Identity=29.1784702549575, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=372, Percent_Identity=24.7311827956989, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=294, Percent_Identity=28.9115646258503, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=255, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=152, Percent_Identity=31.5789473684211, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=57.1428571428571, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=57.1428571428571, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=48.9690721649485, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=28.1879194630872, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=28.1879194630872, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=28.125, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=28.125, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=28.125, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=28.125, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=31.3609467455621, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19922690, Length=301, Percent_Identity=31.8936877076412, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=28.7790697674419, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24647046, Length=282, Percent_Identity=26.9503546099291, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647048, Length=282, Percent_Identity=26.9503546099291, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922512, Length=427, Percent_Identity=22.9508196721311, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24654852, Length=427, Percent_Identity=22.9508196721311, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI78706572, Length=274, Percent_Identity=28.1021897810219, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28572034, Length=138, Percent_Identity=38.4057971014493, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43137; Mature: 43006

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLASFRDYSSIDNTPEEKARG
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCC
ITINASHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKVDMISQEDAELIDLVEMELSELLEEKGYKGCPIIRGSALKALE
EEEHHCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHC
GDANYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVS
CCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEECCCCEEEEC
DKVQLVGLGETKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCQPNSVK
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHCCEEEECCCCCC
PHTKFKSAVYVLQKEEGGRHKPFFSGYRPQFFFRTTDVTGVVTLPEGTEMVMPGDNVELD
CCHHHCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEE
VELIGTVALEEGMRFAIREGGRTIGAGTISKINA
EEEEEHHHHHHCCEEEECCCCCEECCCCCCCCCC
>Mature Secondary Structure 
SKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLASFRDYSSIDNTPEEKARG
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCC
ITINASHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKVDMISQEDAELIDLVEMELSELLEEKGYKGCPIIRGSALKALE
EEEHHCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHC
GDANYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVS
CCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEECCCCEEEEC
DKVQLVGLGETKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCQPNSVK
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHCCEEEECCCCCC
PHTKFKSAVYVLQKEEGGRHKPFFSGYRPQFFFRTTDVTGVVTLPEGTEMVMPGDNVELD
CCHHHCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEE
VELIGTVALEEGMRFAIREGGRTIGAGTISKINA
EEEEEHHHHHHCCEEEECCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA