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The map label for this gene is ptsI

Identifier: 15835575

GI number: 15835575

Start: 51790

End: 53505

Strand: Direct

Name: ptsI

Synonym: CPj0038

Alternate gene names: 15835575

Gene position: 51790-53505 (Clockwise)

Preceding gene: 15835574

Following gene: 15835578

Centisome position: 4.22

GC content: 42.95

Gene sequence:

>1716_bases
ATGGATACACAGTCCTCTATAGGTAACGAAGAATGGCGTATTGCAGGAACCTCTGTAGTTTCTGGGATGGCCTTAGGTAA
AGTATTTTTTTTGGGAACATCCCCCTTGCATGTTCGTGAGCTGACTCTACCTCAAGAAGAAGTCGAACATGAAATACATC
GTTATTATAAAGCTTTGAATCGCTCGAAGTCTGATATCGTAGCTTTAGAACAGGAAGTTACGGGACAGCAAGGCCTTCAA
GAGGTTTCCTCTATCCTACAAGCACACTTGGAGATTATGAAAGACCCTCTCCTTACGGAGGAGGTGGTCAATACTATCCG
TAAGGATCGTAAAAATGCAGAATATGTCTTTTCTTCAGTCATGGGTAAAATAGAAGAGTCGTTAACAGCAGTCCGCGGGA
TGCCTTCTGTTGTAGATCGTGTTCAAGATATCCATGATATCTCCAATAGAGTTATCGGCCATCTGTGTTGCCAACATAAG
AGTTCTTTAGGAGAATCTGATCAGAATTTGATCATATTCTCTGAGGAATTGACCCCCTCAGAAGTCGCCAGTGCTAACTC
TGCCTATATCCGAGGGTTTGTCTCATTAGTGGGAGCAGCCACATCACATACAGCTATCGTCTCGCGAGCAAAGAGCATTC
CCTATCTTGCTAATATCTCCGAGGAGCTTTGGAACATCGCAAAGCGATATAATGGCAAGTTAGTCTTAATCGACGGTTAT
CGTGGAGAGCTAATCTTTAATCCTAAACCAGCGACTCTACAAAGCTGCTATAAAAAAGAGCTTTCCGTGGTTGCCCATAC
CTCTCAGAGATTAGTAAGAAAGTCCCTACACCCGATTGTTTCTTCGCATGCAGGCAGTGATAAGGACGTAGAAGATCTAT
TAGAGAACTTCCCTCAAACCTCCATAGGCCTCTTTCGTTCTGAGTTTTTAGCTGTAATTTTAGGACGCCTACCTACACTA
AGAGAGCAAGTAGATCTTTACGAGAAGCTCGCACGTTTTCCTGGAGATTCGCCCTCAGTACTGCGCCTCTTTGATTTTGG
TGAAGACAAACCTTGTCCTGGAATAAAAAATAAGAAAGAACGTTCTATACGATGGTTGCTAGACTATAGTGTGATTCTTG
AGGATCAGCTCCAAGCAATTGCTAAAGCCTCTTTGCAAGGCTCCATAAAGGTTCTCATTCCAGGAGTGTCTGACGTTTCT
GAGATTATAGAAGTCAAAAAGAAATGGGAGACCATCCAGACGAGGTTCCCTAAAGGCCATAAGGTTTCTTGGGGGACTAT
GATAGAATTTCCTTCTGCAGTTTGGATGATTGAAGAGATCCTTCCTGAATGTGATTTTCTCTCTATAGGGACGAATGACC
TTGTCCAATATACTTTGGGAATTTCCAGGGAATCCGCTCTTCCTAAACATCTAAATGTAACTTTGCCCCCAGCAGTGATC
CGCATGATTCACCATGTACTTCAAGCTGCGAAGCAAAATCAGGTTCCTGTTAGCATTTGTGGAGAGGCCGCAGGGCAGCT
CAGTCTGACTCCTTTATTTATAGGCCTAGGAGTTCAAGAGCTCTCAGTAGCTATGCCTGTAATCAATAGACTTCGCAATC
ATATCGCCCTGCTAGAGTTGAACTCCTGCCTTGAAATTACAGAAGCCCTTTTACAAGCTAAAACATGCTCTGAAGTTGAA
GAACTTTTAAATAGAAACAACAAAATCACATCATAA

Upstream 100 bases:

>100_bases
ACAAGGAGGAGAGATTCTTGTGACTATTAGAAGCAAAGAAGCTCATCGTATCTTACAAAAGATACAAGATGCGTTTAGTT
CCGGTTTTGGAGAACTATAA

Downstream 100 bases:

>100_bases
AAATTCCATTATACTTTTTTTATATAAAGATCTTTTATGATGTATAAAGTTTGTAAAAACTGTGTTTTCTAGTCGTGCCA
ATGTGCAATAGGAAAAAATA

Product: Pts PEP phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MDTQSSIGNEEWRIAGTSVVSGMALGKVFFLGTSPLHVRELTLPQEEVEHEIHRYYKALNRSKSDIVALEQEVTGQQGLQ
EVSSILQAHLEIMKDPLLTEEVVNTIRKDRKNAEYVFSSVMGKIEESLTAVRGMPSVVDRVQDIHDISNRVIGHLCCQHK
SSLGESDQNLIIFSEELTPSEVASANSAYIRGFVSLVGAATSHTAIVSRAKSIPYLANISEELWNIAKRYNGKLVLIDGY
RGELIFNPKPATLQSCYKKELSVVAHTSQRLVRKSLHPIVSSHAGSDKDVEDLLENFPQTSIGLFRSEFLAVILGRLPTL
REQVDLYEKLARFPGDSPSVLRLFDFGEDKPCPGIKNKKERSIRWLLDYSVILEDQLQAIAKASLQGSIKVLIPGVSDVS
EIIEVKKKWETIQTRFPKGHKVSWGTMIEFPSAVWMIEEILPECDFLSIGTNDLVQYTLGISRESALPKHLNVTLPPAVI
RMIHHVLQAAKQNQVPVSICGEAAGQLSLTPLFIGLGVQELSVAMPVINRLRNHIALLELNSCLEITEALLQAKTCSEVE
ELLNRNNKITS

Sequences:

>Translated_571_residues
MDTQSSIGNEEWRIAGTSVVSGMALGKVFFLGTSPLHVRELTLPQEEVEHEIHRYYKALNRSKSDIVALEQEVTGQQGLQ
EVSSILQAHLEIMKDPLLTEEVVNTIRKDRKNAEYVFSSVMGKIEESLTAVRGMPSVVDRVQDIHDISNRVIGHLCCQHK
SSLGESDQNLIIFSEELTPSEVASANSAYIRGFVSLVGAATSHTAIVSRAKSIPYLANISEELWNIAKRYNGKLVLIDGY
RGELIFNPKPATLQSCYKKELSVVAHTSQRLVRKSLHPIVSSHAGSDKDVEDLLENFPQTSIGLFRSEFLAVILGRLPTL
REQVDLYEKLARFPGDSPSVLRLFDFGEDKPCPGIKNKKERSIRWLLDYSVILEDQLQAIAKASLQGSIKVLIPGVSDVS
EIIEVKKKWETIQTRFPKGHKVSWGTMIEFPSAVWMIEEILPECDFLSIGTNDLVQYTLGISRESALPKHLNVTLPPAVI
RMIHHVLQAAKQNQVPVSICGEAAGQLSLTPLFIGLGVQELSVAMPVINRLRNHIALLELNSCLEITEALLQAKTCSEVE
ELLNRNNKITS
>Mature_571_residues
MDTQSSIGNEEWRIAGTSVVSGMALGKVFFLGTSPLHVRELTLPQEEVEHEIHRYYKALNRSKSDIVALEQEVTGQQGLQ
EVSSILQAHLEIMKDPLLTEEVVNTIRKDRKNAEYVFSSVMGKIEESLTAVRGMPSVVDRVQDIHDISNRVIGHLCCQHK
SSLGESDQNLIIFSEELTPSEVASANSAYIRGFVSLVGAATSHTAIVSRAKSIPYLANISEELWNIAKRYNGKLVLIDGY
RGELIFNPKPATLQSCYKKELSVVAHTSQRLVRKSLHPIVSSHAGSDKDVEDLLENFPQTSIGLFRSEFLAVILGRLPTL
REQVDLYEKLARFPGDSPSVLRLFDFGEDKPCPGIKNKKERSIRWLLDYSVILEDQLQAIAKASLQGSIKVLIPGVSDVS
EIIEVKKKWETIQTRFPKGHKVSWGTMIEFPSAVWMIEEILPECDFLSIGTNDLVQYTLGISRESALPKHLNVTLPPAVI
RMIHHVLQAAKQNQVPVSICGEAAGQLSLTPLFIGLGVQELSVAMPVINRLRNHIALLELNSCLEITEALLQAKTCSEVE
ELLNRNNKITS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1788756, Length=574, Percent_Identity=26.1324041811847, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI48994992, Length=506, Percent_Identity=27.0750988142292, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788726, Length=548, Percent_Identity=26.0948905109489, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1789193, Length=543, Percent_Identity=26.8876611418048, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI226510935, Length=179, Percent_Identity=24.0223463687151, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PT1_CHLPN (Q9Z9E3)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A72127
- PIR:   E81544
- PIR:   H86495
- RefSeq:   NP_224246.1
- RefSeq:   NP_300099.1
- RefSeq:   NP_445279.1
- RefSeq:   NP_876318.1
- ProteinModelPortal:   Q9Z9E3
- GeneID:   1466725
- GeneID:   895213
- GeneID:   918830
- GeneID:   963169
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0737
- KEGG:   cpn:CPn0038
- KEGG:   cpt:CpB0042
- TIGR:   CP_0737
- HOGENOM:   HBG414040
- ProtClustDB:   CLSK871320
- BioCyc:   CPNE115711:CP_0737-MONOMER
- BioCyc:   CPNE115713:CPN0038-MONOMER
- BioCyc:   CPNE138677:CPJ0038-MONOMER
- BioCyc:   CPNE182082:CPB0042-MONOMER
- BRENDA:   2.7.3.9
- GO:   GO:0005737
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:1.10.274.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01417

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; SSF47831 PEP-utilisers_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.3.9

Molecular weight: Translated: 63480; Mature: 63480

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 203-203 ACT_SITE 500-500 BINDING 306-306 BINDING 342-342 BINDING 429-429 BINDING 450-450 BINDING 451-451 BINDING 452-452 BINDING 453-453

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQSSIGNEEWRIAGTSVVSGMALGKVFFLGTSPLHVRELTLPQEEVEHEIHRYYKALN
CCCCCCCCCCCEEEEHHHHHHHHHHHHEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHC
RSKSDIVALEQEVTGQQGLQEVSSILQAHLEIMKDPLLTEEVVNTIRKDRKNAEYVFSSV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
MGKIEESLTAVRGMPSVVDRVQDIHDISNRVIGHLCCQHKSSLGESDQNLIIFSEELTPS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHH
EVASANSAYIRGFVSLVGAATSHTAIVSRAKSIPYLANISEELWNIAKRYNGKLVLIDGY
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECC
RGELIFNPKPATLQSCYKKELSVVAHTSQRLVRKSLHPIVSSHAGSDKDVEDLLENFPQT
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCH
SIGLFRSEFLAVILGRLPTLREQVDLYEKLARFPGDSPSVLRLFDFGEDKPCPGIKNKKE
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHH
RSIRWLLDYSVILEDQLQAIAKASLQGSIKVLIPGVSDVSEIIEVKKKWETIQTRFPKGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
KVSWGTMIEFPSAVWMIEEILPECDFLSIGTNDLVQYTLGISRESALPKHLNVTLPPAVI
CCCCCHHHHCCHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCHHHCCCCCCCCCCCHHHH
RMIHHVLQAAKQNQVPVSICGEAAGQLSLTPLFIGLGVQELSVAMPVINRLRNHIALLEL
HHHHHHHHHHHCCCCCHHHHCCCCCCCEECHHHHHCCHHHHHHHHHHHHHHHHHEEHHHH
NSCLEITEALLQAKTCSEVEELLNRNNKITS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDTQSSIGNEEWRIAGTSVVSGMALGKVFFLGTSPLHVRELTLPQEEVEHEIHRYYKALN
CCCCCCCCCCCEEEEHHHHHHHHHHHHEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHC
RSKSDIVALEQEVTGQQGLQEVSSILQAHLEIMKDPLLTEEVVNTIRKDRKNAEYVFSSV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
MGKIEESLTAVRGMPSVVDRVQDIHDISNRVIGHLCCQHKSSLGESDQNLIIFSEELTPS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHH
EVASANSAYIRGFVSLVGAATSHTAIVSRAKSIPYLANISEELWNIAKRYNGKLVLIDGY
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECC
RGELIFNPKPATLQSCYKKELSVVAHTSQRLVRKSLHPIVSSHAGSDKDVEDLLENFPQT
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCH
SIGLFRSEFLAVILGRLPTLREQVDLYEKLARFPGDSPSVLRLFDFGEDKPCPGIKNKKE
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHH
RSIRWLLDYSVILEDQLQAIAKASLQGSIKVLIPGVSDVSEIIEVKKKWETIQTRFPKGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
KVSWGTMIEFPSAVWMIEEILPECDFLSIGTNDLVQYTLGISRESALPKHLNVTLPPAVI
CCCCCHHHHCCHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCHHHCCCCCCCCCCCHHHH
RMIHHVLQAAKQNQVPVSICGEAAGQLSLTPLFIGLGVQELSVAMPVINRLRNHIALLEL
HHHHHHHHHHHCCCCCHHHHCCCCCCCEECHHHHHCCHHHHHHHHHHHHHHHHHEEHHHH
NSCLEITEALLQAKTCSEVEELLNRNNKITS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362