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Identifier: 15835456

GI number: 15835456

Start: 976270

End: 977511

Strand: Direct

Name: Not Available

Synonym: TC0842

Alternate gene names: 15835456

Gene position: 976270-977511 (Clockwise)

Preceding gene: 15835455

Following gene: 15835457

Centisome position: 90.99

GC content: 41.87

Gene sequence:

>1242_bases
TTGATCATGCATAAGAAAACACAATCACCTTCTCAAGAAGGCTTTTCCGTTTGGTCTATTGGAGGCTCTATTTTTGCTAT
GTTCTTTGGAGCAGGCAATGTTGTCTTCCCTTTGGCCTTGGGACACCATTTCTATCATCATACCTCTTACGCTTGTTTAG
GCATGATGCTGACAGCCGTATTGACGCCTTTGTTAGGGCTCTTTGCGATGATGCTATACTCCGGGGACTACCGTTCTTTC
TTCTCTTCCATTGGTAGAATGCCTGGTATGATCGTGATGGTGGCTATTCTATGCATTATTGGACCTTTTGGAGGAGTCCC
TAGAACCATTGCTGTATCTTACGATACGTTGGCTTCGTTAGGTAACACTCAATCTACGCTGTTACCCTCTTTACCGTGGT
TCAGCCTAGTATTTTGTGTACTCGTCTATCTTTTTGTATGTAAGCTTAGCAAACTCATTCAATGGTTAGGATCCGTCTTT
TTCCCTGTCATGCTTGCGACTTTAGCTTGGCTAATTATTAAAGGCTTGTCGATACCAAGTCACGCGCCATCCTTAGAAAA
CGAGCCTTTCTCTAAGCAACAAGCCTTTGTTGCAGGACTAACGGAAGGATTTAACACGATGGACCTGCTGGCAGCGTTTT
TCTTCTGCTCTATCGTTCTTGTGTCCATTCAACAATTTGTTGTTCAGCAAGATCACAAGTCTTCTGAAGAAAAACCCTTA
GAATTTCACCATATTGGTAAGGCTGAAAAATACAAATTAGGGATGAGCTTCCTTCTAGCAGCAGCTCTTCTTAGCCTTGT
CTATCTAGGCTTTGCATACTGCGCAGCTCGTCATGCGGGCGCTCTTGTAAATATTCAACGAGGACAAATTTTAGGTAGAA
TTTCAGCCATTATTTTCGGCCCTAATAGTTTCTTAACGGGGATATGCGTCTTTCTAGCTTGCTTAACCACAGCAATCGCA
GCGACAGGAATTTTTGCAGACTTCATTGCAAGGGTAGTATCCTCACAAAAAATGAGCTACTCCAACGCGATAATAGTTAC
TTTAGTTCCCTCATACTTAGTATCTATTCTAAGTTTTGAGAACATTAGTAAGATCCTCCTTCCTATCTTAGAAGTGAGTT
ATCCCGCACTCATCGCTTTAACTTGTGGCGTCATAGCGAAAAAACTTTGGGGGTTCCGTCAGGCAAAAACTTTATTTTAC
CTTGTTTTTGTGTTGACTCTACTCTATAAACTATCCGTTTAA

Upstream 100 bases:

>100_bases
GCGGACGTTTCTTCTAATTCATCTTTTTTCTCTCCAATACCAGTGATTTTTATCACTCAGGGAGGGATTCGCCTGCTCTT
ATAAACAACATCATTATTTT

Downstream 100 bases:

>100_bases
TGAGCGAATTCGCAATGATTTTTAAGAAACAATCCCTTGCTGAATAAGCAAGGGATCTTCTTTTGCTCAAAGTACGATGC
AAAACAACGCTTTACTTCTC

Product: branched-chain amino acid transport system carrier protein, putative

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MIMHKKTQSPSQEGFSVWSIGGSIFAMFFGAGNVVFPLALGHHFYHHTSYACLGMMLTAVLTPLLGLFAMMLYSGDYRSF
FSSIGRMPGMIVMVAILCIIGPFGGVPRTIAVSYDTLASLGNTQSTLLPSLPWFSLVFCVLVYLFVCKLSKLIQWLGSVF
FPVMLATLAWLIIKGLSIPSHAPSLENEPFSKQQAFVAGLTEGFNTMDLLAAFFFCSIVLVSIQQFVVQQDHKSSEEKPL
EFHHIGKAEKYKLGMSFLLAAALLSLVYLGFAYCAARHAGALVNIQRGQILGRISAIIFGPNSFLTGICVFLACLTTAIA
ATGIFADFIARVVSSQKMSYSNAIIVTLVPSYLVSILSFENISKILLPILEVSYPALIALTCGVIAKKLWGFRQAKTLFY
LVFVLTLLYKLSV

Sequences:

>Translated_413_residues
MIMHKKTQSPSQEGFSVWSIGGSIFAMFFGAGNVVFPLALGHHFYHHTSYACLGMMLTAVLTPLLGLFAMMLYSGDYRSF
FSSIGRMPGMIVMVAILCIIGPFGGVPRTIAVSYDTLASLGNTQSTLLPSLPWFSLVFCVLVYLFVCKLSKLIQWLGSVF
FPVMLATLAWLIIKGLSIPSHAPSLENEPFSKQQAFVAGLTEGFNTMDLLAAFFFCSIVLVSIQQFVVQQDHKSSEEKPL
EFHHIGKAEKYKLGMSFLLAAALLSLVYLGFAYCAARHAGALVNIQRGQILGRISAIIFGPNSFLTGICVFLACLTTAIA
ATGIFADFIARVVSSQKMSYSNAIIVTLVPSYLVSILSFENISKILLPILEVSYPALIALTCGVIAKKLWGFRQAKTLFY
LVFVLTLLYKLSV
>Mature_413_residues
MIMHKKTQSPSQEGFSVWSIGGSIFAMFFGAGNVVFPLALGHHFYHHTSYACLGMMLTAVLTPLLGLFAMMLYSGDYRSF
FSSIGRMPGMIVMVAILCIIGPFGGVPRTIAVSYDTLASLGNTQSTLLPSLPWFSLVFCVLVYLFVCKLSKLIQWLGSVF
FPVMLATLAWLIIKGLSIPSHAPSLENEPFSKQQAFVAGLTEGFNTMDLLAAFFFCSIVLVSIQQFVVQQDHKSSEEKPL
EFHHIGKAEKYKLGMSFLLAAALLSLVYLGFAYCAARHAGALVNIQRGQILGRISAIIFGPNSFLTGICVFLACLTTAIA
ATGIFADFIARVVSSQKMSYSNAIIVTLVPSYLVSILSFENISKILLPILEVSYPALIALTCGVIAKKLWGFRQAKTLFY
LVFVLTLLYKLSV

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=375, Percent_Identity=28, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45155; Mature: 45155

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]